FastQCFastQC Report
Wed 25 May 2016
SRR1295291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631120
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53780.14810857256163387No Hit
GTGGTATCAACGCAGAGTACATGGG53430.14714468263235586No Hit
GGTATCAACGCAGAGTACTTTTTTT44780.12332283152305624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC802.8633242E-511.8772313
TACACCG1002.3958248E-510.4519635
ACCGAGC750.002652169410.1318868
GGTATCA36500.09.7944831
GCTACGA800.0045124479.5013912
ACCGTCA1106.852152E-59.4986438
ATACCGT950.00182728188.998596
CTTGGAC4100.08.8065333
GACTGCG1201.7678985E-48.7069697
GCTTATG6050.08.6435841
GGACCGT1107.159271E-48.6350116
TCTATAC3750.08.6149513
GTGGTAT19000.08.607191
AGGACCG1000.00290140048.5516065
TACACTG6700.08.3672445
TAACACG2053.853347E-88.3430314
GTAAGAC2752.1827873E-118.2924663
TGGTATC18650.08.253222
GTCCTAA3001.8189894E-128.2402171
TTGGACT3700.08.217764
Produced by FastQC (version 0.11.5)