##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295291_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3631120
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.91669897992906	31.0	30.0	33.0	25.0	34.0
2	29.735949514199476	31.0	30.0	34.0	25.0	34.0
3	30.197735960254686	31.0	30.0	34.0	26.0	34.0
4	33.45734373967261	35.0	33.0	37.0	27.0	37.0
5	33.75625206547842	35.0	35.0	37.0	28.0	37.0
6	33.78949359977087	35.0	35.0	37.0	28.0	37.0
7	33.765714710612706	36.0	35.0	37.0	28.0	37.0
8	33.731583919011214	36.0	35.0	37.0	28.0	37.0
9	34.753338363920776	38.0	34.0	39.0	26.0	39.0
10	34.66584029170063	38.0	34.0	39.0	26.0	39.0
11	35.05980303597788	38.0	35.0	39.0	27.0	39.0
12	35.061726684879595	38.0	35.0	39.0	27.0	39.0
13	34.95736769922228	38.0	35.0	39.0	27.0	39.0
14	35.117667276212295	39.0	33.0	41.0	23.0	41.0
15	35.735313897640395	39.0	34.0	41.0	27.0	41.0
16	35.822178005684194	39.0	34.0	41.0	27.0	41.0
17	35.629418196038685	39.0	34.0	41.0	26.0	41.0
18	35.607884619621494	39.0	34.0	41.0	26.0	41.0
19	35.679745367820395	39.0	34.0	41.0	26.0	41.0
20	35.66365033378131	39.0	34.0	41.0	25.0	41.0
21	35.652759754566084	39.0	34.0	41.0	26.0	41.0
22	35.37900289717773	39.0	34.0	40.0	25.0	41.0
23	35.065073861508296	39.0	33.0	40.0	24.0	41.0
24	35.401141521073384	39.0	34.0	41.0	25.0	41.0
25	34.03122314878054	38.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3122730684697963
1101	2	0.5275275589512276
1101	3	0.37534712986108687
1101	4	0.31181803529983654
1101	5	0.24687350380137474
1101	6	0.20902529871912634
1101	7	0.14522968733472652
1101	8	0.16630870002512665
1101	9	0.15004800707281163
1101	10	0.3951144345517221
1101	11	0.34806203179435613
1101	12	0.4149862205754786
1101	13	0.3334025115021859
1101	14	0.8858323608916621
1101	15	0.6149852042543031
1101	16	0.37532985473502833
1101	17	0.7657998616238686
1101	18	0.6948827380536997
1101	19	0.6193926108658232
1101	20	0.8428301456830596
1101	21	0.852964127361588
1101	22	0.5585374560301659
1101	23	-1.62514944422729
1101	24	0.01631919312004726
1101	25	0.34096500395700957
1102	1	0.29821400025193867
1102	2	0.33286076129464703
1102	3	0.23000623712211166
1102	4	0.35578037990909905
1102	5	0.1050941005353323
1102	6	0.1644476543702993
1102	7	0.11686969003252301
1102	8	0.08791296990012398
1102	9	0.34988915394522024
1102	10	0.7963197027110098
1102	11	0.6137565935865084
1102	12	0.42637448121111277
1102	13	0.4974804095201648
1102	14	0.8718932945396034
1102	15	0.6571305280923525
1102	16	0.48268562244347635
1102	17	0.8039725572777456
1102	18	0.8809876486557187
1102	19	0.7195699951043366
1102	20	0.8229503834049865
1102	21	0.7714407963772203
1102	22	0.7197130546025363
1102	23	0.7518988633486643
1102	24	0.7601202858025005
1102	25	0.568353589715052
1103	1	-0.617969809281032
1103	2	-1.670144856589289
1103	3	-0.5636391410818717
1103	4	-0.1943117846192095
1103	5	-0.31139809758850845
1103	6	-0.35280280689240584
1103	7	-0.27269085795429504
1103	8	-0.08958586566654958
1103	9	0.4491218192593891
1103	10	0.5530703512826136
1103	11	0.2139583050598901
1103	12	0.03506176675593764
1103	13	-0.1520766154625477
1103	14	-1.3962796764121492
1103	15	-0.4562238892009418
1103	16	-0.3480620371068355
1103	17	0.09495191909976342
1103	18	0.17955627421702047
1103	19	0.24386495579398826
1103	20	0.30291486921015576
1103	21	0.4103993878142944
1103	22	0.07639735452082874
1103	23	0.7748931917772595
1103	24	0.5286725894945121
1103	25	0.2360125000518991
1104	1	-0.09125257615195181
1104	2	0.018027261816712326
1104	3	-0.3221301691802587
1104	4	-0.29127690092772696
1104	5	-0.2401494807064921
1104	6	-0.19795277054488025
1104	7	-0.03918058160597582
1104	8	-0.11210149750483822
1104	9	-2.258015351959756
1104	10	-0.6822724285769439
1104	11	-0.5688848836565796
1104	12	-0.06313621789597335
1104	13	0.0670212192740749
1104	14	-1.7879336326441049
1104	15	-0.6109608609485591
1104	16	-0.23900098878016962
1104	17	0.14402718181833052
1104	18	0.31225061564380496
1104	19	0.22991087540846422
1104	20	0.461843379077159
1104	21	0.5240954355004845
1104	22	0.6481916225254167
1104	23	0.9171108641475385
1104	24	0.6869650744674374
1104	25	0.5365379793850948
1105	1	-0.4483554824219915
1105	2	-0.38439249632113004
1105	3	-1.673863977241055
1105	4	-0.864850684677883
1105	5	-0.7700463638718489
1105	6	-0.5295336919564306
1105	7	-0.38253427749326363
1105	8	-0.3925807233927969
1105	9	0.013550171915397868
1105	10	0.14578153578020903
1105	11	-0.4273065105552476
1105	12	-0.4570122575586879
1105	13	-0.5644913284618767
1105	14	0.09828282058662552
1105	15	-0.1478895250118839
1105	16	-0.6916816795277754
1105	17	-0.1446377429048269
1105	18	-0.06741470628075064
1105	19	-0.13698717304053787
1105	20	-0.6206692308710586
1105	21	-0.29558679141158706
1105	22	-0.23364783871633676
1105	23	-0.12507922304534702
1105	24	-0.12918391434392618
1105	25	0.01411434494438879
1106	1	-1.7684045351843665
1106	2	-1.1447805513862015
1106	3	-1.1476944726477107
1106	4	-1.4020137131535222
1106	5	-0.9749788964245383
1106	6	-0.9179633969084264
1106	7	-0.7379447958924601
1106	8	-0.8689264582419867
1106	9	-0.43269594293028035
1106	10	-1.9848488186766602
1106	11	-1.3608527314144396
1106	12	-1.0043395037199971
1106	13	-1.00293959868619
1106	14	-0.1952878083350953
1106	15	-0.9920536402981384
1106	16	-1.4655440779458289
1106	17	-0.7274435901696421
1106	18	-0.7353555572801369
1106	19	-0.6346556501977219
1106	20	-0.36511814991184366
1106	21	-0.29070528646715843
1106	22	-0.33541575603268114
1106	23	-0.7562001696634155
1106	24	-0.9205952278444727
1106	25	-1.6373134170002075
1107	1	-0.07338003460062126
1107	2	0.1175116255426687
1107	3	-0.021433687289544423
1107	4	0.08530476494701134
1107	5	-0.16747895745258035
1107	6	-0.034635686500273266
1107	7	-0.08624234314955004
1107	8	-0.12360337928812015
1107	9	0.4064854412301244
1107	10	-0.038049306316757736
1107	11	-0.13789449830792222
1107	12	-0.1883516948616233
1107	13	0.0918485234424935
1107	14	-1.0480123398744894
1107	15	-0.5530911016374844
1107	16	-0.32692732310321304
1107	17	-0.07241728273869796
1107	18	0.2832524651631587
1107	19	0.2754834679954712
1107	20	0.21574459111152322
1107	21	0.3139064023876301
1107	22	-0.32522960278324575
1107	23	0.47605748452175334
1107	24	0.2898069820450999
1107	25	0.1119144826957097
1108	1	-0.04585435263573956
1108	2	0.21943654382443967
1108	3	-0.09855886013234993
1108	4	-0.41243226871302596
1108	5	-0.23865046432256776
1108	6	-0.27669519752006266
1108	7	-0.19692723061985618
1108	8	-0.13599055298212193
1108	9	0.2829492004488614
1108	10	0.41785520233315054
1108	11	0.08550421807458264
1108	12	0.015146741525512653
1108	13	-0.05186054260948936
1108	14	0.07054189179758197
1108	15	-0.02009480044030454
1108	16	0.00464580435593831
1108	17	0.2974543518834949
1108	18	0.37025064654176987
1108	19	0.21521359072490043
1108	20	0.4268835473556365
1108	21	0.3794053150486931
1108	22	0.24854586949653168
1108	23	0.11493039884287981
1108	24	0.03491304974831877
1108	25	-0.6526853147729739
1109	1	-0.7491676761947126
1109	2	-0.6518286825073183
1109	3	-0.7688031269170459
1109	4	-0.5963794502855961
1109	5	-0.9261529309652587
1109	6	-1.2713665116181971
1109	7	-0.9478665834834672
1109	8	-1.3185219277550573
1109	9	-1.0119915540210656
1109	10	-1.1084564522077756
1109	11	-1.251050781141629
1109	12	-1.2010556430494361
1109	13	-1.2318411783117256
1109	14	-0.6823160019200927
1109	15	-1.2330378970681508
1109	16	-1.3939926088781718
1109	17	-1.4340956218363914
1109	18	-1.9980599567944282
1109	19	-2.061433988276825
1109	20	-1.8132186223678204
1109	21	-1.7018280985081589
1109	22	-1.7521840723183644
1109	23	-1.1562887220180045
1109	24	-2.1993123322355075
1109	25	-1.1432831183083394
1110	1	0.1828376624772936
1110	2	0.39917231849368306
1110	3	0.11245109026309663
1110	4	0.4888467017924114
1110	5	0.3314693209162485
1110	6	0.31691918148231224
1110	7	0.3144866957216621
1110	8	0.18333437299013156
1110	9	0.4672222894520388
1110	10	0.5214245398954418
1110	11	0.329461953815553
1110	12	0.4583954747350134
1110	13	0.43113624355458313
1110	14	-2.3939537507800637
1110	15	-0.45957054082301596
1110	16	0.019889235929596794
1110	17	-0.13733682600351926
1110	18	-2.764363482542393
1110	19	-0.32566488584105713
1110	20	-1.7999974382207071
1110	21	-0.2565891558618105
1110	22	-1.6614937523687843
1110	23	-0.318715964074876
1110	24	0.23462104558676344
1110	25	0.22903790642008914
1111	1	-0.11401806726549424
1111	2	-1.4268279866518334
1111	3	-0.40440643362230944
1111	4	0.13434266214609636
1111	5	-0.025652471700638557
1111	6	0.152750540552411
1111	7	0.06738434200433119
1111	8	0.12452499146075979
1111	9	-1.4242569811371624
1111	10	-0.13236456217149595
1111	11	-0.060133643612751086
1111	12	-0.011212349704599944
1111	13	0.08480349932162312
1111	14	0.907149117163982
1111	15	0.38192596483700925
1111	16	0.4898294005669044
1111	17	0.09847690950030596
1111	18	0.4017845426999216
1111	19	0.09590635927979463
1111	20	0.39090622338421355
1111	21	-0.38852185714836196
1111	22	0.012428243395909533
1111	23	0.029789529250770386
1111	24	-0.2976931229128965
1111	25	-1.9051309393528655
1112	1	0.3311677380406337
1112	2	0.46814396320213447
1112	3	0.1165305892528572
1112	4	0.46900897446541023
1112	5	0.41744048424570224
1112	6	0.2877264126974879
1112	7	0.2942237659475282
1112	8	0.2231588482917246
1112	9	-0.11191202281062118
1112	10	0.615550117761785
1112	11	0.272834577829407
1112	12	0.19073899600359567
1112	13	0.2401646383342566
1112	14	0.6883257433206964
1112	15	0.3515947959401444
1112	16	0.38190986659250115
1112	17	-3.2903355492209343
1112	18	-0.6802851634952987
1112	19	-0.9305968111408092
1112	20	0.20763281851789372
1112	21	-0.23355512368274844
1112	22	-1.3787395311356434
1112	23	-1.046256860135557
1112	24	-0.04339330834526578
1112	25	0.45575343023472215
1113	1	-0.18924779372084188
1113	2	0.05196977672389025
1113	3	0.012875756523442305
1113	4	-0.3195564548705221
1113	5	-0.03978311275391633
1113	6	-0.006583663815455054
1113	7	-0.13069560532966307
1113	8	-0.1363235824960043
1113	9	0.3376284928239386
1113	10	0.5057183142948247
1113	11	0.015043022234948467
1113	12	0.07478225912752379
1113	13	-0.23890304037783494
1113	14	-1.3056195707499327
1113	15	-1.4581641319580285
1113	16	-0.35313968657992234
1113	17	-0.14418629404234906
1113	18	-0.47047756059104273
1113	19	-0.0037703562720921013
1113	20	0.33781835577678976
1113	21	-1.4758161590053192
1113	22	-1.9301330942871076
1113	23	-0.5397457549734241
1113	24	-2.26504298722309
1113	25	-0.5784575003080903
1114	1	0.3351627934290704
1114	2	0.33251954703190734
1114	3	0.053867128604505154
1114	4	0.41906774315614115
1114	5	0.22809648215864087
1114	6	0.34830526793762573
1114	7	0.30697720300425146
1114	8	0.3399382445591428
1114	9	0.7396263189678862
1114	10	0.5377163167412533
1114	11	0.5230467201796145
1114	12	0.4329272484368616
1114	13	0.3037347591537056
1114	14	1.1364802568215993
1114	15	0.6166039548792952
1114	16	0.6454041070737944
1114	17	-0.12935667415747076
1114	18	0.0975820426402052
1114	19	-0.26121429382111216
1114	20	-1.4346765946648077
1114	21	-0.5568257018413689
1114	22	-1.3718912487990735
1114	23	-2.6733253357121853
1114	24	-1.8552381215992213
1114	25	-1.2784835165237425
1115	1	0.5439597238865659
1115	2	0.6209205492383667
1115	3	0.4873578389144697
1115	4	0.7349926313753912
1115	5	0.6413320806555873
1115	6	0.5943265610794413
1115	7	0.720715034119948
1115	8	0.7072472169598569
1115	9	0.954465800444396
1115	10	1.2039962539195344
1115	11	0.8911898274345091
1115	12	0.7147834093836352
1115	13	0.6989435141354079
1115	14	1.3987026142632502
1115	15	0.9398887751692158
1115	16	0.9055734244638316
1115	17	0.7146082771969589
1115	18	1.0093843247907586
1115	19	0.4509740609279831
1115	20	1.0017480219247261
1115	21	0.0126757210742241
1115	22	1.0523247243647873
1115	23	1.1516846847389814
1115	24	1.2100905526564816
1115	25	1.3251152141345912
1116	1	0.6532911347124042
1116	2	0.7555014637404831
1116	3	0.5700773302897844
1116	4	0.9182432532597744
1116	5	0.7394280199539196
1116	6	0.8010850914798056
1116	7	0.7519578409075223
1116	8	0.7181775276716422
1116	9	1.006878369853034
1116	10	0.8381990190309594
1116	11	0.7758878862713985
1116	12	0.8041154709407863
1116	13	0.22159496034906567
1116	14	1.2572649219133822
1116	15	0.9609631291794116
1116	16	0.9335614459282624
1116	17	1.2403738367653574
1116	18	1.1936107050148692
1116	19	1.24614399224253
1116	20	1.1063904706068968
1116	21	0.538639039750862
1116	22	0.6080407992878349
1116	23	-0.24096182003668076
1116	24	0.17623519792368825
1116	25	-0.28547985989710156
1201	1	0.39563749080272004
1201	2	0.5549637076124938
1201	3	0.5604493394549657
1201	4	0.3510299882412653
1201	5	0.30259491585282206
1201	6	0.21722007425820777
1201	7	0.30461461712118165
1201	8	0.3475489107315326
1201	9	0.7027935112854635
1201	10	0.935069748700478
1201	11	0.6704786731086472
1201	12	0.6442939633856568
1201	13	0.5968419851639197
1201	14	1.1345347757820576
1201	15	0.7661116320281707
1201	16	0.5276062585241661
1201	17	0.6659935414666833
1201	18	0.6339990763430023
1201	19	0.8643070418629293
1201	20	0.8951579983110349
1201	21	0.9179956841251311
1201	22	0.8750526642408261
1201	23	0.47678606969460446
1201	24	0.8151195952577908
1201	25	0.8344082232638073
1202	1	0.23723818751092196
1202	2	0.5643150620117119
1202	3	0.39092312988014655
1202	4	0.33155570895391406
1202	5	0.2565316300259326
1202	6	0.1266234057767548
1202	7	0.1281824879431781
1202	8	0.14853586406245967
1202	9	0.655965679748526
1202	10	-0.04171785302066411
1202	11	0.20250729374367893
1202	12	0.21884806632795772
1202	13	0.16991698189037407
1202	14	0.8954912297086537
1202	15	0.5630004542562475
1202	16	0.4354141222676873
1202	17	-1.546288469498343
1202	18	-0.10967423609749005
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2210	17	0.7644848425611954
2210	18	0.6562914705757734
2210	19	0.7481498162957863
2210	20	1.01448273836953
2210	21	1.0414707160632588
2210	22	1.2735484002954607
2210	23	1.5096470777390465
2210	24	1.2581398263118828
2210	25	1.196069294204051
2211	1	0.18593943228574616
2211	2	-0.5092275493899798
2211	3	0.010804376315906694
2211	4	0.3876536378531341
2211	5	0.39699291424540917
2211	6	0.29789881613452707
2211	7	0.32856037339967514
2211	8	0.40952132043224765
2211	9	0.8438655687508216
2211	10	0.8946316710724247
2211	11	0.5238315887947635
2211	12	0.3061935886895881
2211	13	0.2914308022951886
2211	14	-0.6922380556836814
2211	15	0.11207407822389825
2211	16	0.3858319387014717
2211	17	0.4377746706821597
2211	18	0.43265756573582337
2211	19	0.8458915674280831
2211	20	0.7909590796009951
2211	21	0.7983573965392807
2211	22	0.01810007222748311
2211	23	0.5104269217684703
2211	24	-0.05030041858973533
2211	25	0.4804908391510878
2212	1	0.11091066301406016
2212	2	-0.94937466124642
2212	3	-0.20696197332665278
2212	4	0.32753714578469584
2212	5	0.3697329852846565
2212	6	0.30252594504473507
2212	7	0.2286274636475909
2212	8	0.1940520618057917
2212	9	-1.963365860582428
2212	10	0.07258400179526348
2212	11	0.1768376128135003
2212	12	0.11661895773941922
2212	13	0.22646614822613742
2212	14	0.9245783950046444
2212	15	0.5169213847617087
2212	16	0.5158761274010786
2212	17	0.5761003172776498
2212	18	0.2468207580964048
2212	19	-0.20420537442535647
2212	20	0.6080779826294958
2212	21	0.551108619807728
2212	22	-1.5486832206071952
2212	23	-0.12081927886403321
2212	24	0.4429897473972133
2212	25	-0.02823360975023803
2213	1	0.32170291874938783
2213	2	0.564491926003182
2213	3	0.2675624576568083
2213	4	0.4416379239033006
2213	5	0.3724658716045397
2213	6	0.35109457121042453
2213	7	0.30957557536757463
2213	8	0.35530577437280186
2213	9	0.8050964988097107
2213	10	0.742509245496052
2213	11	0.16311511522947342
2213	12	0.2261364801610597
2213	13	0.20534234518113692
2213	14	-1.4997372823122745
2213	15	-0.2633292297484573
2213	16	0.11627591385589398
2213	17	-1.8352066869597081
2213	18	-0.6604563807835007
2213	19	-0.3395419584215489
2213	20	0.37287384069248475
2213	21	0.029662227959192933
2213	22	0.5103589607606551
2213	23	1.0250057424311336
2213	24	0.7539281057522516
2213	25	-0.684862431158713
2214	1	0.43552471869635667
2214	2	0.3696492906262847
2214	3	0.3437320164790236
2214	4	0.6362800660825343
2214	5	0.6106542174975189
2214	6	0.5659838392420866
2214	7	0.5398855700965015
2214	8	0.38131753473341234
2214	9	0.9532476230061917
2214	10	0.8731422223710297
2214	11	0.2404735909125435
2214	12	0.2433987451135593
2214	13	0.34444553122001764
2214	14	1.1455027898722108
2214	15	0.846965866246876
2214	16	0.8761843128219269
2214	17	0.7575378863534112
2214	18	0.7777837830228265
2214	19	-0.5073092573309026
2214	20	0.36616915052988475
2214	21	0.8110928305717096
2214	22	0.9908138211431066
2214	23	1.4089570637575832
2214	24	1.1912337614894
2214	25	1.264246448956328
2215	1	0.11866675416239403
2215	2	0.5360328226740556
2215	3	0.39393695014443963
2215	4	0.6867971235156602
2215	5	0.6162975256525769
2215	6	0.5727783726122624
2215	7	0.46651480457924777
2215	8	0.5412229583256192
2215	9	0.5852649768551785
2215	10	1.0379608053715543
2215	11	0.6251897037382577
2215	12	0.5972592729276371
2215	13	0.3840451444306652
2215	14	1.3899335675192788
2215	15	0.7370994043612953
2215	16	0.670251909472114
2215	17	0.855949754705648
2215	18	0.69548127911888
2215	19	0.13303741278365777
2215	20	-1.0446563479592328
2215	21	-1.4657842298919803
2215	22	0.2584399283723826
2215	23	0.9330290571679924
2215	24	1.0296146505405304
2215	25	0.4959483020560569
2216	1	0.07166543414334825
2216	2	-0.2374151908319604
2216	3	-0.10481560234912024
2216	4	0.37762711346491074
2216	5	0.4830961747467981
2216	6	0.2647856049914168
2216	7	0.31505788417299385
2216	8	0.26010146860135563
2216	9	0.5106874650457982
2216	10	-1.3279165883425463
2216	11	-0.2590670459554332
2216	12	-0.02970299986340308
2216	13	0.170669041023352
2216	14	-0.1971817587660425
2216	15	-0.199030102998897
2216	16	0.20508735876444462
2216	17	0.5425277068524892
2216	18	0.3529849221575887
2216	19	-1.0663592617920585
2216	20	-0.7821677763698958
2216	21	-0.5965050014267845
2216	22	-0.35959914187750996
2216	23	-4.070685296810616
2216	24	-1.7886286133510367
2216	25	-0.8216232952896902
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	17.0
4	52.0
5	55.0
6	80.0
7	307.0
8	220.0
9	634.0
10	1809.0
11	4043.0
12	7401.0
13	11855.0
14	17001.0
15	21275.0
16	24660.0
17	26850.0
18	28331.0
19	28772.0
20	29645.0
21	29903.0
22	31362.0
23	32774.0
24	34443.0
25	37666.0
26	42381.0
27	47525.0
28	55038.0
29	66950.0
30	80773.0
31	99640.0
32	128967.0
33	168583.0
34	224632.0
35	305143.0
36	424631.0
37	621749.0
38	995952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92845283218564	21.241805745735018	13.59915461094243	23.23058681113691
2	15.941748744150456	21.506766038335602	39.823094409971766	22.728390807542176
3	18.075646739456825	26.49798428530421	30.265472683305518	25.160896291933447
4	12.685376533147167	17.696028908118482	38.13594320423109	31.482651354503272
5	13.364769520160976	37.34721492449226	35.484904651312085	13.803110904034673
6	30.44076941489574	38.41504916612197	17.762220464862864	13.381960954119423
7	25.585484931923432	32.68399735120061	24.170129167534355	17.560388549341603
8	23.852796262192022	36.61630304674877	21.507685399325332	18.023215291733873
9	24.5883560883408	17.274374451947256	22.250493609047307	35.886775850664634
10	15.177969832148005	29.801786226847838	33.73666954803315	21.28357439297101
11	31.64289659294845	25.889983238138264	23.790604818105564	18.676515350807723
12	21.07653308704743	27.524735784825598	31.47397760454641	19.924753523580563
13	26.3256454578636	24.438133147850998	26.937268864448683	22.298952529836715
14	21.27997086354491	23.518155322981848	28.1197922650095	27.082081548463737
15	22.821680901273485	30.73449138623086	24.475892631338837	21.96793508115682
16	22.21536010331009	31.11968478082417	25.65205127159777	21.012903844267978
17	20.35056606403483	30.06834528707858	28.498119822832795	21.082968826053794
18	20.357393238617973	29.87632827618503	29.334811682632377	20.431466802564614
19	21.09922558129606	28.64393404150708	29.632319826799048	20.624520550397815
20	22.409532293152335	28.29986982588179	28.85596065799904	20.434637222966835
21	22.899900265765158	28.77474523149993	27.680946299132746	20.644408203602165
22	21.816518603316194	28.259780940429064	29.295333773318188	20.62836668293656
23	22.12688478892017	28.604691519895987	28.51134041064572	20.757083280538126
24	21.73330648624394	27.75828364464113	28.93384460328054	21.574565265834387
25	22.331377886783336	28.182388629700473	28.304689046503384	21.181544437012803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2753.0
1	2753.0
2	3126.0
3	3499.0
4	3499.0
5	3499.0
6	5136.5
7	6774.0
8	6774.0
9	6774.0
10	10379.5
11	13985.0
12	13985.0
13	13985.0
14	22639.0
15	31293.0
16	31293.0
17	31293.0
18	50398.5
19	69504.0
20	69504.0
21	69504.0
22	100786.0
23	132068.0
24	132068.0
25	132068.0
26	173865.0
27	215662.0
28	215662.0
29	215662.0
30	260833.5
31	306005.0
32	306005.0
33	306005.0
34	344940.0
35	383875.0
36	383875.0
37	383875.0
38	411597.0
39	439319.0
40	439319.0
41	439319.0
42	440219.0
43	441119.0
44	441119.0
45	441119.0
46	423572.0
47	406025.0
48	406025.0
49	406025.0
50	387942.5
51	369860.0
52	369860.0
53	369860.0
54	334931.5
55	300003.0
56	300003.0
57	300003.0
58	260919.0
59	221835.0
60	221835.0
61	221835.0
62	182917.0
63	143999.0
64	143999.0
65	143999.0
66	112021.0
67	80043.0
68	80043.0
69	80043.0
70	58812.5
71	37582.0
72	37582.0
73	37582.0
74	26933.0
75	16284.0
76	16284.0
77	16284.0
78	11285.5
79	6287.0
80	6287.0
81	6287.0
82	4313.0
83	2339.0
84	2339.0
85	2339.0
86	1548.0
87	757.0
88	757.0
89	757.0
90	473.0
91	189.0
92	189.0
93	189.0
94	115.5
95	42.0
96	42.0
97	42.0
98	30.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.081737866002776
2	0.019525655995946155
3	0.031450351406728506
4	0.03891361343056688
5	0.048387274449756545
6	0.01853422635440305
7	0.021921610963008657
8	0.03263455903412721
9	0.03368106810020049
10	0.024730661614047456
11	0.023546453986648747
12	0.01792835268457115
13	0.02602502809050651
14	0.03588424508140739
15	0.03398400493511644
16	0.02434510564233625
17	0.024152327656480645
18	0.02602502809050651
19	0.018038511533631497
20	0.01646874793452158
21	0.012971204476855624
22	0.019057480887439686
23	0.02037938707616383
24	0.01880962347705391
25	0.10977329308863382
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3631120.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.2053525178338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.78464432892378	72.0978834090075
2	8.473465075059169	13.761814369354655
3	1.5125120319735728	3.6847221823163725
4	0.46296425514237816	1.5038070216797268
5	0.21128135473618592	0.8578588445898736
6	0.12371780329484847	0.6027928697574113
7	0.09705533468035776	0.5516988866518355
8	0.05507471166593445	0.3577889100520208
9	0.04154259940460811	0.3036133286242519
>10	0.2152615920956591	3.095860510354074
>50	0.01408194387737148	0.7765336241922103
>100	0.007276139624398704	1.0542590256408204
>500	6.80502751691819E-4	0.38834291987607794
>1k	3.742764977684064E-4	0.6668003831611311
>5k	6.805027232152837E-5	0.29622371474207443
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5378	0.14810857256163387	No Hit
GTGGTATCAACGCAGAGTACATGGG	5343	0.14714468263235586	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4478	0.12332283152305624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.7539712265086255E-5	0.0	0.0	0.0
3	0.0	2.7539712265086255E-5	0.0	0.0	0.0
4	0.0	2.7539712265086255E-5	0.0	0.0	0.0
5	0.0	2.7539712265086255E-5	0.0	0.0	0.0
6	0.0	2.7539712265086255E-5	0.0	0.0	0.0
7	0.0	2.7539712265086255E-5	0.0	0.0	0.0
8	0.0	2.7539712265086255E-5	0.0	2.7539712265086255E-5	0.0
9	0.0	2.7539712265086255E-5	0.0	2.7539712265086255E-5	0.0
10	0.0	2.7539712265086255E-5	0.0	2.7539712265086255E-5	0.0
11	0.0	2.7539712265086255E-5	0.0	2.7539712265086255E-5	0.0
12	0.0	2.7539712265086255E-5	0.0	2.7539712265086255E-5	0.0
13	0.0	2.7539712265086255E-5	0.0	2.7539712265086255E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGAC	80	2.8633242E-5	11.877231	3
TACACCG	100	2.3958248E-5	10.451963	5
ACCGAGC	75	0.0026521694	10.131886	8
GGTATCA	3650	0.0	9.794483	1
GCTACGA	80	0.004512447	9.501391	2
ACCGTCA	110	6.852152E-5	9.498643	8
ATACCGT	95	0.0018272818	8.99859	6
CTTGGAC	410	0.0	8.806533	3
GACTGCG	120	1.7678985E-4	8.706969	7
GCTTATG	605	0.0	8.643584	1
GGACCGT	110	7.159271E-4	8.635011	6
TCTATAC	375	0.0	8.614951	3
GTGGTAT	1900	0.0	8.60719	1
AGGACCG	100	0.0029014004	8.551606	5
TACACTG	670	0.0	8.367244	5
TAACACG	205	3.853347E-8	8.343031	4
GTAAGAC	275	2.1827873E-11	8.292466	3
TGGTATC	1865	0.0	8.25322	2
GTCCTAA	300	1.8189894E-12	8.240217	1
TTGGACT	370	0.0	8.21776	4
>>END_MODULE