FastQCFastQC Report
Wed 25 May 2016
SRR1295291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631120
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT132320.3644054726916213No Hit
GGTATCAACGCAGAGTACTTTTTTT107880.29709841591575054No Hit
TATCAACGCAGAGTACTTTTTTTTT76320.2101830840071383No Hit
GTGGTATCAACGCAGAGTACATGGG60410.16636740179338605No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52120.14353698032562956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC752.0623537E-411.4076662
AGCGTCA3050.010.9089713
CAGCGTC3250.010.5301542
TACACCG1002.38305E-510.4570285
GGTATCA45050.010.4164281
GTCTTAC3300.010.3837731
GTGGTAT21650.09.6723381
GCAGCGT3350.09.6605261
TGCACGG1002.738562E-49.506395
TGTGCGA800.00453086069.49670210
TCTGTCG2500.09.4967028
TGGTATC21450.09.439912
TATTCCG1752.644083E-89.2347775
CCTATAC3400.09.2267893
CTAGACT3950.08.9047184
ATCCGAA1502.7168717E-68.86358812
TATACTG7300.08.8552675
GTCTCGC2605.456968E-128.786271
CGCGATC1201.7715544E-48.70531117
ACACCGT1752.6420093E-78.6826996
Produced by FastQC (version 0.11.5)