FastQCFastQC Report
Wed 25 May 2016
SRR1295290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101761
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA985854.690590414419147No Hit
GCTTATGTACTCTGCGTTGATACCA566672.696167642277119No Hit
CTTATGTACTCTGCGTTGATACCAC390201.8565383980385972No Hit
GAGTACATAAGCAGTGGTATCAACG252971.2036097348842232No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA252421.2009928816835027No Hit
TATGTACTCTGCGTTGATACCACTG237951.1321458529299955No Hit
CCCATGTACTCTGCGTTGATACCAC197740.940830094382758No Hit
GCGTTGATACCACTGCTTATGTACT192080.9139002959898865No Hit
CATAAGCAGTGGTATCAACGCAGAG166150.7905275623631802No Hit
ACTCTGCGTTGATACCACTGCTTAT147850.703457719502836No Hit
CTGCTTATGTACTCTGCGTTGATAC142070.6759569713207163No Hit
ACGCAGAGTACATAAGCAGTGGTAT140240.6672499870346819No Hit
CATGTACTCTGCGTTGATACCACTG129090.614199235783707No Hit
GTATCAACGCAGAGTACATAAGCAG123170.5860323795141312No Hit
GTACATGATAAGCAGTGGTATCAAC117820.5605775347434842No Hit
ATCATGTACTCTGCGTTGATACCAC113570.5403563963742786No Hit
GTATCAACGCAGAGTACTTTTTTTT109630.5216102116272974No Hit
TATCAACGCAGAGTACATAAGCAGT108870.5179941962953922No Hit
GGTATCAACGCAGAGTACATAAGCA102410.48725806597419974No Hit
GTACATGGGAAGCAGTGGTATCAAC83310.3963818911855344No Hit
AAGCAGTGGTATCAACGCAGAGTAC82950.39466904181778995No Hit
GTGGTATCAACGCAGAGTACATAAG77890.3705939923711592No Hit
GGTATCAACGCAGAGTACTTTTTTT77320.36788198087223045No Hit
GTGGTATCAACGCAGAGTACATGGG72370.34433030206574394No Hit
TATCAACGCAGAGTACTTTTTTTTT71850.3418561863123352No Hit
GTACTGGTTCACTATCGGTCAGTCA68930.32796307477396336No Hit
GTACTTTTTTTTTTTTTTTTTTTTT67580.3215398896449216No Hit
ACATAAGCAGTGGTATCAACGCAGA66920.3183996658040567No Hit
GTACTCTGCGTTGATACCACTGCTT59520.2831910954670869No Hit
GCAGTGGTATCAACGCAGAGTACAT58430.27800496821474946No Hit
CCACTGCTTATGTACTCTGCGTTGA57360.27291399926062No Hit
GATACCACTGCTTATGTACTCTGCG54510.25935394176597626No Hit
GTACATGGGATAAGCAGTGGTATCA49210.23413699274084923No Hit
GTTGATACCACTGCTTATGTACTCT48120.2289508654885118No Hit
ATACCACTGCTTATGTACTCTGCGT47710.22700012037524722No Hit
GGATACCACGTGTCCCGCCCTACTC46270.2201487229042693No Hit
ATACAGGGTGACAGCCCCGTACACA45070.21443922501178775No Hit
GAGTACATGATAAGCAGTGGTATCA43040.20478065774367304No Hit
GCTTATCCCATGTACTCTGCGTTGA42530.20235412113936835No Hit
CAGTGGTATCAACGCAGAGTACATA38670.18398856958521925No Hit
TACCACTGCTTATGTACTCTGCGTT37210.17704201381603332No Hit
TGATACCACTGCTTATGTACTCTGC36710.17466305636083265No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35420.16852534612641495No Hit
GCTTATCATGTACTCTGCGTTGATA35090.16695523420598252No Hit
GAGTACATGGGAAGCAGTGGTATCA34690.16505206824182198No Hit
GCAGAGTACATAAGCAGTGGTATCA32970.15686845459593168No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA32140.1529193852202986No Hit
GATAAGCAGTGGTATCAACGCAGAG31980.1521581188346344No Hit
GCGTTGATACCACTGCTTATCATGT31560.15015979457226583No Hit
GGTATCAACGCAGAGTACATGGGGG30490.14506882561813642No Hit
GTTAATGATAGTGTGTCGAAACACA29890.14221407667189562No Hit
GGATAAGCAGTGGTATCAACGCAGA29500.1403584898568391No Hit
CCCCATGTACTCTGCGTTGATACCA29390.13983511921669495No Hit
GTATCAACGCAGAGTACATGGGAAG28710.13659973707762205No Hit
GTATTTAGCCTTGGAGGATGGTCCC28230.13431593792062943No Hit
CCCATATTCAGACAGGATACCACGT28020.13331677578944512No Hit
GCGTTGATACCACTGCTTCCCATGT27400.1303668685449963No Hit
ACCCTGTATCGCGCGCCTTTCCAGA27340.13008139365037222No Hit
GTATCAACGCAGAGTACATGGGGGT26940.1281782276862117No Hit
GAACAAAAAAAAAAAAAAAAAAAAA26870.12784517364248363No Hit
ACGCAGAGTACATGATAAGCAGTGG26760.12732180300233945No Hit
GGTATCAACGCAGAGTACATGGGAA26730.12717906555502742No Hit
GGGATAAGCAGTGGTATCAACGCAG25470.12118409276792176No Hit
TATCAACGCAGAGTACATGGGAAGC25130.11956640169838531No Hit
GTACACAAGCAGTGGTATCAACGCA25110.11947124340017728No Hit
ATCAACGCAGAGTACATAAGCAGTG24850.11823418552347294No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24510.11661649445393649No Hit
TATCAACGCAGAGTACATGATAAGC24510.11661649445393649No Hit
GTTCACTATCGGTCAGTCAGGAGTA24100.11466574934067195No Hit
GAGTACATGGGATAAGCAGTGGTAT24070.1145230118933599No Hit
GTACATGGGGGTTAAGCGACTAAGC23610.11233437103457529No Hit
GCCTTGGAGGATGGTCCCCCCATAT23200.11038362592131075No Hit
CAGTGGTATCAACGCAGAGTACATG23120.11000299272847865No Hit
CTATCGGTCAGTCAGGAGTATTTAG23100.10990783443027062No Hit
ATCATTAACTGAATCCATAGGTTAA22910.10900383059729436No Hit
TATTCAGACAGGATACCACGTGTCC22680.10790951016790207No Hit
GTATCAACGCAGAGTACATGATAAG22400.1065772939929897No Hit
AAAAAGTACTCTGCGTTGATACCAC22220.10572086930911745No Hit
ACGCAGAGTACATGGGAAGCAGTGG22170.10548297356359737No Hit
ATGTACTCTGCGTTGATACCACTGC21790.10367496589764487No Hit
GGTATCAACGCAGAGTACATGATAA21550.10253306631914855No Hit
ACTTAGATGTTTCAGTTCCCCCGGT21470.10215243312631644No Hit
ACTCTGCGTTGATACCACTGCTTCC21040.10010652971484389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACC307.7346456E-418.99609614
CGGTAGG453.512206E-516.904331
TCCTATA350.002166620716.2901232
GCACCGA551.1303751E-515.5430016
CTTCGTT2750.015.196877512
AGTCGTA508.731552E-515.196877511
AGTTGTA3550.014.98283711
TCGCCTA456.774273E-414.77474212
TTCGTTG2900.014.41083213
GTACAAA7600.014.3881431
CAGTTGT3700.014.37542410
GCAGTTG3700.014.3754249
CTATAGA400.00524800714.2630281
TAATACT602.5625595E-514.2538584
TCGTTAG602.5729361E-514.2477518
ATTTCGT400.005285454414.247756
CTCTTCG2800.014.24707210
GTTGTAT3850.013.81534312
CTACGCA903.615787E-813.72953419
ACTCTTC3050.013.7021029
Produced by FastQC (version 0.11.5)