FastQCFastQC Report
Wed 25 May 2016
SRR1295290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101761
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1047124.982107860979436No Hit
GCTTATGTACTCTGCGTTGATACCA637453.032932859635325No Hit
CTTATGTACTCTGCGTTGATACCAC453012.1553830335609043No Hit
GTATCAACGCAGAGTACTTTTTTTT282991.3464423404944712No Hit
GAGTACATAAGCAGTGGTATCAACG278371.3244607736084169No Hit
CCCATGTACTCTGCGTTGATACCAC239551.1397585167866375No Hit
TATGTACTCTGCGTTGATACCACTG224681.06900832206897No Hit
GCGTTGATACCACTGCTTATGTACT218671.040413253457458No Hit
CATAAGCAGTGGTATCAACGCAGAG212171.0094868065398492No Hit
GGTATCAACGCAGAGTACTTTTTTT204810.9744685527992955No Hit
TATCAACGCAGAGTACTTTTTTTTT192340.915137353866591No Hit
ACTCTGCGTTGATACCACTGCTTAT173880.8273062446205826No Hit
ACGCAGAGTACATAAGCAGTGGTAT171430.8156493530900992No Hit
CTGCTTATGTACTCTGCGTTGATAC166790.793572627905837No Hit
CATGTACTCTGCGTTGATACCACTG150010.7137348157093028No Hit
GTACTTTTTTTTTTTTTTTTTTTTT138640.6596373231780398No Hit
GTATCAACGCAGAGTACATAAGCAG137410.6537850878382461No Hit
ATCATGTACTCTGCGTTGATACCAC128200.6099646915134499No Hit
TATCAACGCAGAGTACATAAGCAGT123720.5886492327148519No Hit
GTACATGATAAGCAGTGGTATCAAC117010.5567236236660591No Hit
GGTATCAACGCAGAGTACATAAGCA114100.5428780912767912No Hit
ACGCAGAGTACTTTTTTTTTTTTTT109230.5197070456631367No Hit
AAGCAGTGGTATCAACGCAGAGTAC96590.459567001195664No Hit
ACATAAGCAGTGGTATCAACGCAGA83250.39609641629091036No Hit
GTACATGGGAAGCAGTGGTATCAAC82340.3917667137224451No Hit
GTGGTATCAACGCAGAGTACATAAG79050.37611317366722474No Hit
GTGGTATCAACGCAGAGTACATGGG74150.35279939060625826No Hit
GTACTGGTTCACTATCGGTCAGTCA70780.3367652173582058No Hit
GCAGTGGTATCAACGCAGAGTACAT69750.33186456500049244No Hit
CCACTGCTTATGTACTCTGCGTTGA65770.3129280636570952No Hit
GATACCACTGCTTATGTACTCTGCG63340.3013663304248199No Hit
GTACTCTGCGTTGATACCACTGCTT59240.2818588792921745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA57550.2738180030935963No Hit
GAGTACTTTTTTTTTTTTTTTTTTT57160.27196241627853973No Hit
ATACAGGGTGACAGCCCCGTACACA53670.2553572932412391No Hit
ATACCACTGCTTATGTACTCTGCGT52830.251360644716502No Hit
GGATACCACGTGTCCCGCCCTACTC51620.24560356767491642No Hit
GTACATGGGATAAGCAGTGGTATCA49210.23413699274084923No Hit
GCTTATCCCATGTACTCTGCGTTGA48420.23037823996163218No Hit
GAGTACATGATAAGCAGTGGTATCA48280.229712131874176No Hit
GTTGATACCACTGCTTATGTACTCT48280.229712131874176No Hit
CAGTGGTATCAACGCAGAGTACATA46790.22262283865767798No Hit
TACCACTGCTTATGTACTCTGCGTT42810.20368633731428074No Hit
GCAGAGTACTTTTTTTTTTTTTTTT42140.20049853432431183No Hit
TGATACCACTGCTTATGTACTCTGC40470.19255281642394165No Hit
GCAGAGTACATAAGCAGTGGTATCA39460.1877473223644363No Hit
GCTTATCATGTACTCTGCGTTGATA38980.18546352320744366No Hit
GAGTACATGGGAAGCAGTGGTATCA38460.18298940745403497No Hit
GATAAGCAGTGGTATCAACGCAGAG36600.1741396857206885No Hit
GGATAAGCAGTGGTATCAACGCAGA35800.17033335379236747No Hit
CCCCATGTACTCTGCGTTGATACCA35160.16728828824971062No Hit
GCGTTGATACCACTGCTTATCATGT34680.16500448909271798No Hit
CCCATATTCAGACAGGATACCACGT33600.15986594098948453No Hit
ACGCAGAGTACATGATAAGCAGTGG32820.1561547673593715No Hit
GGTATCAACGCAGAGTACATGGGGG32330.15382338905327483No Hit
GTTAATGATAGTGTGTCGAAACACA32230.1533475975622347No Hit
GTATCAACGCAGAGTACATGGGAAG32100.15272906862388255No Hit
ACCCTGTATCGCGCGCCTTTCCAGA32080.15263391032567453No Hit
GGGATAAGCAGTGGTATCAACGCAG31180.14835178690631332No Hit
GCGTTGATACCACTGCTTCCCATGT29970.14259470986472772No Hit
GGTATCAACGCAGAGTACATGGGAA29870.1421189183736876No Hit
ATCAACGCAGAGTACATAAGCAGTG29640.14102459794429528No Hit
GTATTTAGCCTTGGAGGATGGTCCC28820.1371231077177662No Hit
GTATCAACGCAGAGTACATGGGGGT28750.1367900536740381No Hit
CAGTGGTATCAACGCAGAGTACATG28540.13579089154285381No Hit
TATCAACGCAGAGTACATGATAAGC28300.1346489919643575No Hit
GTACACAAGCAGTGGTATCAACGCA27920.132840984298405No Hit
GTGGTATCAACGCAGAGTACTTTTT27780.1321748762109488No Hit
ACGCAGAGTACATGGGAAGCAGTGG27410.13041444769410032No Hit
ATCAACGCAGAGTACTTTTTTTTTT27340.13008139365037222No Hit
CTATCGGTCAGTCAGGAGTATTTAG27190.12936770641381204No Hit
TATTCAGACAGGATACCACGTGTCC26830.12765485704606755No Hit
TATCAACGCAGAGTACATGGGAAGC26680.12694116980950734No Hit
GCCTTGGAGGATGGTCCCCCCATAT26500.1260847451256351No Hit
AAAAAGTACTCTGCGTTGATACCAC26390.12556137448549098No Hit
GAGTACATGGGATAAGCAGTGGTAT25800.12275420468835418No Hit
GTCAGGAGTATTTAGCCTTGGAGGA25150.11966155999659334No Hit
ACTCTGCGTTGATACCACTGCTTCC25070.11928092680376122No Hit
ATCATTAACTGAATCCATAGGTTAA24950.11870997701451307No Hit
CGGTACTGGTTCACTATCGGTCAGT24490.11652133615572846No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG24140.114856065937088No Hit
ATGATAAGCAGTGGTATCAACGCAG24050.11442785359515187No Hit
TACATAAGCAGTGGTATCAACGCAG23840.1134286914639676No Hit
GTATCAACGCAGAGTACATGATAAG23820.11333353316575957No Hit
GTTCACTATCGGTCAGTCAGGAGTA23650.11252468763099134No Hit
GGTATCAACGCAGAGTACATGATAA23340.11104973400876694No Hit
ACTTAGATGTTTCAGTTCCCCCGGT23310.11090699656145489No Hit
ATGTACTCTGCGTTGATACCACTGC22490.1070055063349258No Hit
TCACGGTACTGGTTCACTATCGGTC21920.10429349483599705No Hit
GCCCAGAGCCTGAATCAGTGTGTGT21450.10205727482810842No Hit
GTACATGGGGGTTAAGCGACTAAGC21220.10096295439871612No Hit
GCGTTGATACCACTGCTTATCCCAT21130.10053474205678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT359.803736E-519.0346411
TGTAGGA250.00601250319.0110722
GGACTCG250.006043993418.99388717
TGGTACG250.006043993418.99388710
CGGACTC250.006043993418.99388716
TCCTAGG402.749451E-416.634692
TTACCCC1050.016.2952064
ACTCGCG350.002170723216.28551119
CGCTAAT350.002175212816.28047414
CCCTAAG551.1093674E-515.5737971
CCTAGGG456.73392E-414.786393
CCATACC456.73392E-414.786394
GAGATAC456.73392E-414.786393
TACCGCC456.780255E-414.7730237
CCCCGCT653.3847755E-614.6106827
CTTATAC2400.014.275981
GTGTAGG500.001479405613.3242481
CAACGGT500.001503495613.2957217
AGATTGC500.001503495613.295728
GTCCTAA655.361841E-513.1778291
Produced by FastQC (version 0.11.5)