FastQCFastQC Report
Wed 25 May 2016
SRR1295289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822590
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG46530.16484859650179445No Hit
GTATCAACGCAGAGTACTTTTTTTT31100.11018249196659805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGG600.00587183811.0848125
GGTATCA25250.010.6195991
TAGACTG3150.010.2553375
GTGGTAT11900.010.2278291
TGGTATC11750.010.1882322
TACCCCG856.588406E-410.0601665
TAGGACG900.00111274729.5012674
ACGGATC800.0045229759.4985738
TAGGACC1451.7042785E-69.1736374
TTAGGAC2501.8189894E-129.1210553
GGCCTAG2201.6552804E-109.0764711
GCACCGT1054.5010916E-49.046266
TTAGACT3050.09.0339924
CCGTAAT950.00182691258.99864914
AGCTTCG850.00739095458.9480819
GCCTTAC1502.6700236E-68.8747721
TGTCGCC2252.6193447E-108.8656510
TAGACAG4050.08.6801715
AGCACCG1000.00290241868.5511415
CTAGCAC2002.5349436E-88.5509893
Produced by FastQC (version 0.11.5)