##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295289_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2822590
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59665661679521	31.0	30.0	33.0	27.0	34.0
2	30.43399891588931	31.0	30.0	34.0	26.0	34.0
3	30.919452701242477	31.0	31.0	34.0	27.0	34.0
4	34.16128839115847	35.0	35.0	37.0	30.0	37.0
5	34.409296072047304	37.0	35.0	37.0	30.0	37.0
6	34.55903655862169	37.0	35.0	37.0	31.0	37.0
7	34.59710974672198	37.0	35.0	37.0	31.0	37.0
8	34.6685076472318	37.0	35.0	37.0	31.0	37.0
9	35.71309010518708	39.0	35.0	39.0	29.0	39.0
10	35.66375208585023	39.0	35.0	39.0	29.0	39.0
11	36.02940774253434	39.0	35.0	39.0	30.0	39.0
12	36.04935892212472	39.0	35.0	39.0	30.0	39.0
13	35.95927640925533	39.0	35.0	39.0	30.0	39.0
14	36.25227432960508	39.0	36.0	41.0	27.0	41.0
15	36.86160264154553	40.0	36.0	41.0	30.0	41.0
16	36.96075271293387	40.0	36.0	41.0	30.0	41.0
17	36.75493004651756	40.0	36.0	41.0	29.0	41.0
18	36.72569058913976	40.0	36.0	41.0	29.0	41.0
19	36.79454756092808	40.0	36.0	41.0	29.0	41.0
20	36.780011974817455	40.0	36.0	41.0	29.0	41.0
21	36.77844214002034	40.0	36.0	41.0	29.0	41.0
22	36.53228240729259	39.0	36.0	41.0	27.0	41.0
23	36.215803216195056	39.0	35.0	41.0	27.0	41.0
24	36.54271821270535	39.0	36.0	41.0	27.0	41.0
25	35.40610786547107	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5151569448083535
1101	2	0.6757509063572975
1101	3	0.5413501558837552
1101	4	0.505956875031309
1101	5	0.4066165843372218
1101	6	0.41572951510960365
1101	7	0.3814580131384844
1101	8	0.4416349034549327
1101	9	0.3895824828823464
1101	10	0.5916782278420456
1101	11	0.4843830986007518
1101	12	0.6188279622872415
1101	13	0.5977584325535261
1101	14	1.134840332505533
1101	15	0.8934232692404862
1101	16	0.6491469843688975
1101	17	1.007759134131419
1101	18	1.0490253576242523
1101	19	0.8888244819669708
1101	20	1.09732975460539
1101	21	1.1066080450974027
1101	22	0.7365479390955514
1101	23	-1.4544058746295008
1101	24	0.309615069969972
1101	25	0.7111983027310202
1102	1	0.4790840975410937
1102	2	0.5448774702964805
1102	3	0.5466881831535737
1102	4	0.7059143430591419
1102	5	0.4114950225256351
1102	6	0.3612575185217537
1102	7	0.3749006760535707
1102	8	0.4762283234780895
1102	9	0.7438941927825198
1102	10	1.2503465125287079
1102	11	0.813204499386309
1102	12	0.7967991882141376
1102	13	0.7624724617400318
1102	14	1.4583045731279896
1102	15	1.1716849754489616
1102	16	0.9244760126846785
1102	17	1.1883041588248417
1102	18	1.1997345085723907
1102	19	1.24161969776452
1102	20	1.251293295803201
1102	21	1.3101802811611378
1102	22	1.1293378456401086
1102	23	1.2576260975841436
1102	24	1.329059234942072
1102	25	1.3269156898152303
1103	1	-0.17409771060820134
1103	2	-1.6294473947668777
1103	3	-0.28723490653387884
1103	4	0.007789315398234464
1103	5	-0.060221603034200655
1103	6	0.04750833891435491
1103	7	0.16679158908263503
1103	8	0.3093650713600553
1103	9	0.8956017815870538
1103	10	0.948517913313836
1103	11	0.6310671638064775
1103	12	0.5219930261579648
1103	13	0.3367209798299484
1103	14	-0.9841151593396802
1103	15	0.01993570229479502
1103	16	0.15438401320936634
1103	17	0.6210363112045272
1103	18	0.7895064728796086
1103	19	0.7824206708339148
1103	20	0.7720348335085561
1103	21	0.8872444655676688
1103	22	0.3985042695769181
1103	23	1.155600878609789
1103	24	0.9931746936145842
1103	25	0.9840124531998811
1104	1	0.14044505655454387
1104	2	0.19350623469611605
1104	3	-0.20490603446374678
1104	4	0.013791348333221265
1104	5	-0.012734997957402072
1104	6	-0.01861097595406136
1104	7	0.1783174518312407
1104	8	0.19896544737478905
1104	9	-1.9323186366511038
1104	10	-0.24881730490771048
1104	11	-0.13055544088997806
1104	12	0.23494471175475695
1104	13	0.3548869210072354
1104	14	-1.4402618387889206
1104	15	-0.34302473972714154
1104	16	0.10628782854328023
1104	17	0.43386414303542864
1104	18	0.5191808241368321
1104	19	0.5530566159892061
1104	20	0.7870315506013981
1104	21	0.8165289001343083
1104	22	0.8793402162969315
1104	23	1.2197456948361989
1104	24	0.9524729639347527
1104	25	0.8868633165352549
1105	1	-0.11204008211939609
1105	2	0.005529735959719062
1105	3	-1.2092995143383014
1105	4	-0.33604419385258666
1105	5	-0.2681931937048745
1105	6	-0.0015385684705577773
1105	7	0.012499220315113746
1105	8	0.054709534298844176
1105	9	0.43345560258303095
1105	10	0.5580999828566533
1105	11	0.08604806752384064
1105	12	0.03804294153466259
1105	13	-0.1099186375055794
1105	14	0.679157967533591
1105	15	0.31733255385594106
1105	16	-0.15783455915835276
1105	17	0.4861493807799846
1105	18	0.3336038404295181
1105	19	0.15850966463968774
1105	20	-0.23200983411140186
1105	21	0.23675144829045536
1105	22	0.30128878697302497
1105	23	0.4666477436740806
1105	24	0.38500068127202525
1105	25	0.7705630322571295
1106	1	-1.3717028173675807
1106	2	-1.0584370210727236
1106	3	-1.057789931182743
1106	4	-1.305358304025333
1106	5	-0.9586149000197395
1106	6	-0.7590162359118722
1106	7	-0.5328434011116272
1106	8	-0.67539214586143
1106	9	-0.22178738296744172
1106	10	-1.7532862005780245
1106	11	-1.1068490791495194
1106	12	-0.83592063231022
1106	13	-0.8780263904144263
1106	14	-0.16175156275387081
1106	15	-0.9138642921277409
1106	16	-1.3283444989151434
1106	17	-0.7271672537389762
1106	18	-0.5085684372033867
1106	19	-0.4713206502164198
1106	20	-0.312030345860002
1106	21	-0.3030476797496746
1106	22	-0.2653685579116569
1106	23	-0.5930620024499618
1106	24	-0.6260880282124432
1106	25	-1.260447492934368
1107	1	0.10950956215080865
1107	2	0.32831334952796
1107	3	0.1832789126246439
1107	4	0.34033574109420783
1107	5	0.054340170251741426
1107	6	0.15368817671079427
1107	7	0.0424804741895386
1107	8	0.03747075459400406
1107	9	0.535523843583988
1107	10	0.1965004056280435
1107	11	0.20077749277380263
1107	12	0.03670120593903192
1107	13	0.11632487498457067
1107	14	-0.8933029851268586
1107	15	-0.25487485293711387
1107	16	-0.07174943707460812
1107	17	0.10573748703851038
1107	18	0.43546218436935646
1107	19	0.36803137792710316
1107	20	0.4818899979922193
1107	21	0.6437343534826994
1107	22	-0.24693814641389622
1107	23	0.7601402436288964
1107	24	0.6616689322680784
1107	25	0.1758372541546862
1108	1	-0.1870350499900617
1108	2	0.19586579155290096
1108	3	-0.18637516256393383
1108	4	-0.3431871736557355
1108	5	-0.3943653575416377
1108	6	-0.2989632211151516
1108	7	-0.2582253091159572
1108	8	-0.3090538669401539
1108	9	0.1252281756173943
1108	10	0.24499467636611172
1108	11	-0.07551020100871142
1108	12	-0.20845500112137216
1108	13	-0.31225737280520605
1108	14	0.02399215251421083
1108	15	-0.17290118722969083
1108	16	-0.2756652982193941
1108	17	0.15484733059707878
1108	18	0.02116876270358148
1108	19	0.07676502124505191
1108	20	0.19952019658396125
1108	21	0.12462558528090995
1108	22	0.03403102577278361
1108	23	-0.11341503292621269
1108	24	-0.13601574187584475
1108	25	-0.8948730603550032
1109	1	-0.8296353197326987
1109	2	-0.7746883228124837
1109	3	-0.8621656478993813
1109	4	-0.6581085056177187
1109	5	-0.9372778687801286
1109	6	-1.1385497405302019
1109	7	-0.9055512486389006
1109	8	-1.1965756737078763
1109	9	-0.8299882064034989
1109	10	-1.0623661765609356
1109	11	-1.1902081552879125
1109	12	-1.1055587575952046
1109	13	-1.1264211000772946
1109	14	-0.6441556404141977
1109	15	-1.2257042305166266
1109	16	-1.2935991012162162
1109	17	-1.384373856673129
1109	18	-1.9880198985485436
1109	19	-2.160336838865419
1109	20	-1.9013395257681225
1109	21	-1.682020086561316
1109	22	-1.7722629816625428
1109	23	-1.2342687369584553
1109	24	-2.2297624084246124
1109	25	-1.456521143099657
1110	1	0.06361355235961952
1110	2	0.12730073251838903
1110	3	0.046824325999200056
1110	4	0.22923307209701704
1110	5	0.17765188262434606
1110	6	0.11236269828621204
1110	7	0.023769984218397155
1110	8	-0.011760846692027371
1110	9	0.22346320259049435
1110	10	0.1927910946417697
1110	11	0.11213392422986601
1110	12	0.17364273203202885
1110	13	0.0496175364587117
1110	14	-2.325408240405153
1110	15	-0.633092265233735
1110	16	-0.22014452052054168
1110	17	-0.3242047988447325
1110	18	-2.7486941303110655
1110	19	-0.5496754777763755
1110	20	-1.9828650912924957
1110	21	-0.3496977650595525
1110	22	-1.714650054909697
1110	23	-0.44021981460921467
1110	24	0.03945760577909141
1110	25	-0.13969802369275186
1111	1	-0.3020537722105203
1111	2	-1.3481747196060816
1111	3	-0.49695435723710446
1111	4	-0.10815386154349227
1111	5	-0.08951572378749262
1111	6	-0.1060010483039946
1111	7	-0.15320855088503293
1111	8	-0.11414164056706966
1111	9	-1.846623570821329
1111	10	-0.5021094440678553
1111	11	-0.3501175225848172
1111	12	-0.30627315927439014
1111	13	-0.12903608782183795
1111	14	0.662167124283819
1111	15	0.1436736471812523
1111	16	0.1307240684902382
1111	17	-0.24219586685471484
1111	18	-0.050459101434796594
1111	19	-0.1828982025662782
1111	20	0.11193917196565906
1111	21	-0.6676898243810925
1111	22	-0.3357107964798658
1111	23	-0.18548380087855065
1111	24	-0.614558743040341
1111	25	-1.9997519328525968
1112	1	0.1100771681860806
1112	2	0.27629569787638175
1112	3	-0.10321427543560802
1112	4	0.293380355462439
1112	5	0.0715863276618407
1112	6	0.10605188310002944
1112	7	0.02590473825123496
1112	8	-0.05996148111979949
1112	9	-0.3684506379773964
1112	10	0.3337831800146489
1112	11	0.08306945238739871
1112	12	0.024735100236945584
1112	13	-0.06344283934772221
1112	14	0.4075962805316422
1112	15	0.060222047156024416
1112	16	0.0948564714048743
1112	17	-3.8254444757877764
1112	18	-1.0334654368046188
1112	19	-1.2884716388503534
1112	20	-0.17535722713498103
1112	21	-0.5941204598717746
1112	22	-1.730598404553568
1112	23	-1.4113863630231762
1112	24	-0.43873069898819494
1112	25	-0.11413392701688707
1113	1	-0.4631564814710032
1113	2	-0.1633671436560178
1113	3	-0.18642958785007124
1113	4	-0.6129946999732425
1113	5	-0.20304644146806794
1113	6	-0.2984331795451425
1113	7	-0.37531086276191417
1113	8	-0.3748502980669173
1113	9	0.09340495845030006
1113	10	0.24477015556161774
1113	11	-0.25824804896866027
1113	12	-0.24612904654277656
1113	13	-0.5280683716055563
1113	14	-1.6396489756845725
1113	15	-1.9077699677328184
1113	16	-0.711212516092921
1113	17	-0.47809793622013785
1113	18	-0.7357078084030775
1113	19	-0.31947477665367074
1113	20	0.05312585639133971
1113	21	-1.8514327445465355
1113	22	-2.2752634315683977
1113	23	-0.94200173627884
1113	24	-2.580368944647738
1113	25	-0.9646287660564923
1114	1	-0.04180709351281564
1114	2	0.028531500885371486
1114	3	-0.19224089895568852
1114	4	0.10898622655845003
1114	5	-0.11159652359541639
1114	6	-0.02971851545538584
1114	7	-0.019137564664504225
1114	8	-0.1097388707359741
1114	9	0.22716563210583018
1114	10	-0.014892203896053502
1114	11	0.07966231017181968
1114	12	-0.0032323719967877196
1114	13	0.05125754669541749
1114	14	0.7078607392042002
1114	15	0.13609571869832848
1114	16	0.08103267357498822
1114	17	-0.5377241702544424
1114	18	-0.2967202589070652
1114	19	-0.7746394220018971
1114	20	-1.8839170070798232
1114	21	-1.0299122957856994
1114	22	-1.6985097189349005
1114	23	-2.9932522306657745
1114	24	-2.156746028613995
1114	25	-1.7048417478145907
1115	1	0.07690202302566362
1115	2	0.16730491786347912
1115	3	-0.022633395002891632
1115	4	0.2197514569819461
1115	5	0.12400435806773658
1115	6	0.1508802372442517
1115	7	0.13445316983439426
1115	8	0.13434036041464026
1115	9	0.3190614301300698
1115	10	0.40614515047737854
1115	11	0.17064146855429385
1115	12	-0.006596902964545848
1115	13	-0.07847948973180507
1115	14	0.5766939912621822
1115	15	0.158842198315007
1115	16	0.11400878121479252
1115	17	0.1078438722159305
1115	18	0.33629310327916784
1115	19	-0.26498454004500616
1115	20	0.3354796748485782
1115	21	-0.7807063741741587
1115	22	0.18239968278653151
1115	23	0.3901373348386059
1115	24	0.3860910972500733
1115	25	0.288851602910988
1116	1	0.29646429080391457
1116	2	0.417205466877288
1116	3	0.1984808996899723
1116	4	0.6484367814916183
1116	5	0.5641986387117655
1116	6	0.4062797972937915
1116	7	0.3244178628857668
1116	8	0.1817195126781712
1116	9	0.4310595076574728
1116	10	0.411502748865459
1116	11	0.33857203771709266
1116	12	0.24768817753899697
1116	13	-0.11350453733148669
1116	14	0.7434342910939691
1116	15	0.37882371300158724
1116	16	0.3596499443107106
1116	17	0.5812150388240553
1116	18	0.5268587538697744
1116	19	0.6099434596592275
1116	20	0.5115319513149288
1116	21	0.012178443837932207
1116	22	-0.006368271074130405
1116	23	-0.8813987947064703
1116	24	-0.47096955306325583
1116	25	-1.0538871811250985
1201	1	0.37644833220402774
1201	2	0.5660733287477662
1201	3	0.48524997505976586
1201	4	0.45461395884886
1201	5	0.42361781024826684
1201	6	0.27803538165067465
1201	7	0.39010636577537383
1201	8	0.3512616023322934
1201	9	0.6191858942338015
1201	10	0.8692422547023355
1201	11	0.4305714288918949
1201	12	0.46605795582514986
1201	13	0.475343583843177
1201	14	1.1562249244105445
1201	15	0.6790279461816411
1201	16	0.4409553208829351
1201	17	0.5936891291608006
1201	18	0.5760772437065498
1201	19	0.8626878314853812
1201	20	0.9202695153684104
1201	21	0.7852124688643372
1201	22	0.9721539902502485
1201	23	0.5454073636526502
1201	24	0.8318452487687509
1201	25	0.9514449745312135
1202	1	0.49085935620939125
1202	2	0.7436384544879608
1202	3	0.6353393711762614
1202	4	0.4456708720840865
1202	5	0.4281249368531235
1202	6	0.37939509636230895
1202	7	0.39968902058429734
1202	8	0.4783835211715157
1202	9	0.921279719702838
1202	10	0.30301891648410617
1202	11	0.5546519366424576
1202	12	0.5656553955364316
1202	13	0.5254310564099569
1202	14	1.2574889021439617
1202	15	1.0115064664207836
1202	16	0.8149503579288861
1202	17	-1.2991135498300252
1202	18	0.2875301027646344
1202	19	0.7133724071667089
1202	20	0.9350577460046665
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2210	23	1.4013025203053502
2210	24	0.9746582173268123
2210	25	0.9232114816999584
2211	1	-0.028366563940988243
2211	2	-0.7868864126780366
2211	3	-0.19998538169116387
2211	4	0.13109359515576102
2211	5	0.18069738714731187
2211	6	0.15341132199377938
2211	7	0.1104855577985191
2211	8	0.15503321362788824
2211	9	0.6334713459847947
2211	10	0.7144001205801018
2211	11	0.31871283871834777
2211	12	0.17475491537305032
2211	13	0.0691732584426461
2211	14	-0.9728176414687937
2211	15	-0.13913605288223607
2211	16	0.11989804855866737
2211	17	0.22109558803998652
2211	18	0.16956599821933338
2211	19	0.4696140037216594
2211	20	0.48231528342123653
2211	21	0.42973365991685597
2211	22	-0.15178110329087247
2211	23	0.29654863958639766
2211	24	-0.2950104497723771
2211	25	0.08959805777647034
2212	1	-0.05523551477908484
2212	2	-1.125231666314292
2212	3	-0.29876452559152256
2212	4	0.10923548477121159
2212	5	0.18789533908648792
2212	6	0.15594719397276435
2212	7	0.1198692791248206
2212	8	0.047609983513346776
2212	9	-2.068704778804822
2212	10	-0.008496431578102204
2212	11	0.07393762818474414
2212	12	0.014603458168778616
2212	13	-0.01363361747044678
2212	14	0.6448623681869066
2212	15	0.226264101653463
2212	16	0.3712097878664977
2212	17	0.34393032036445703
2212	18	0.13370072972928426
2212	19	-0.4470295541685161
2212	20	0.27803030537241824
2212	21	0.21925249076209496
2212	22	-1.8292885783167279
2212	23	-0.3738262298135382
2212	24	0.18237255255935736
2212	25	-0.3018828192636107
2213	1	0.18717800400706253
2213	2	0.504973063787812
2213	3	0.09948625527143662
2213	4	0.3702130493435618
2213	5	0.4151227497581047
2213	6	0.35622970256921604
2213	7	0.23624914385341356
2213	8	0.18560850276852392
2213	9	0.6101630853316209
2213	10	0.5288894437066105
2213	11	-0.006516311160787325
2213	12	0.09916952408519109
2213	13	0.0587074635428948
2213	14	-1.7813115920304838
2213	15	-0.36639214972064593
2213	16	-0.009067266415023312
2213	17	-2.1307909361103654
2213	18	-0.9489416687475369
2213	19	-0.42961115269869055
2213	20	0.25022511657287083
2213	21	-0.0688369306884411
2213	22	0.3513087910069004
2213	23	0.9294429788289449
2213	24	0.5611754119615213
2213	25	-1.1426091280793358
2214	1	0.11036997111974856
2214	2	0.00397989653551889
2214	3	-0.04165675087247678
2214	4	0.32409637425821813
2214	5	0.27827207064358817
2214	6	0.26814615410563647
2214	7	0.111051393995524
2214	8	-0.007423384982821801
2214	9	0.4876819224207125
2214	10	0.36619069060299125
2214	11	-0.1837225957865698
2214	12	-0.1905054390248182
2214	13	-0.10898823564390625
2214	14	0.6954183073430471
2214	15	0.21388393798180516
2214	16	0.20556868976467513
2214	17	0.15348748538096402
2214	18	0.09665143171988433
2214	19	-1.1059560266100306
2214	20	0.01595149961613629
2214	21	0.36395619010160374
2214	22	0.3808855796191608
2214	23	0.9599132544067359
2214	24	0.7837213164363277
2214	25	0.6413094135216753
2215	1	-0.3586348129733885
2215	2	0.13137890660132712
2215	3	0.03305557641461121
2215	4	0.18640333425656053
2215	5	0.17546188152645215
2215	6	0.07476827343701586
2215	7	8.989485531571972E-4
2215	8	0.06335260383509933
2215	9	0.23557404983279184
2215	10	0.4635044365274439
2215	11	0.12144523588292344
2215	12	0.04419235264783339
2215	13	-0.0678692763234281
2215	14	0.7561898165642162
2215	15	0.24897917263447766
2215	16	0.13848601391286053
2215	17	0.3562624927263158
2215	18	0.2102219767477962
2215	19	-0.4446724349639055
2215	20	-1.7551121513728134
2215	21	-1.9666523402294231
2215	22	-0.25267572228725044
2215	23	0.3466781051882819
2215	24	0.375971281402137
2215	25	-0.37430784478426204
2216	1	-0.2117456646971938
2216	2	-0.4607823544785745
2216	3	-0.39465996542613624
2216	4	-0.0052112268676864915
2216	5	0.07592887093473166
2216	6	-0.15284067399812074
2216	7	-0.15723976588177635
2216	8	-0.256196786167024
2216	9	-0.05223647302965162
2216	10	-1.8651872181657296
2216	11	-0.797162914631258
2216	12	-0.4891487979776059
2216	13	-0.2974723251780631
2216	14	-0.85951488648724
2216	15	-0.831443280350598
2216	16	-0.31689895943115687
2216	17	-0.01852030987070208
2216	18	-0.1941211225422066
2216	19	-1.6153863185669408
2216	20	-1.332850739156541
2216	21	-1.3307808225608966
2216	22	-1.101578244590982
2216	23	-4.815519896549482
2216	24	-2.3005052872949747
2216	25	-1.3902530448900308
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	27.0
5	41.0
6	53.0
7	203.0
8	140.0
9	280.0
10	809.0
11	1760.0
12	3377.0
13	5541.0
14	7987.0
15	10111.0
16	11936.0
17	13144.0
18	13831.0
19	14376.0
20	14279.0
21	14946.0
22	15665.0
23	16638.0
24	18213.0
25	19924.0
26	22657.0
27	26797.0
28	32157.0
29	39629.0
30	50004.0
31	62838.0
32	84923.0
33	116055.0
34	162092.0
35	230689.0
36	338896.0
37	527975.0
38	944586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.004260612172246	21.26987233764926	13.764479202567713	22.96138784761078
2	15.741330342604945	21.608260646939907	40.38447159959675	22.2659374108584
3	17.870675674909418	26.657717017186727	30.630340729316103	24.84126657858775
4	12.948176953562768	17.627909548372735	38.69035678362395	30.733556714440546
5	13.578429565523148	37.25159788347786	35.421120985441824	13.748851565557175
6	30.354266340764408	38.56654975667177	17.92301799289469	13.156165909669134
7	25.522720314333824	32.661071989476845	24.569591150572933	17.24661654561639
8	23.151016803428874	37.21200728925898	21.804445994345226	17.83252991296693
9	24.311609110713476	17.42594594939414	22.587074620909675	35.67537031898271
10	15.012615613593677	29.871753074169888	34.02328218576137	21.09234912647507
11	31.43591986058026	26.007288494398615	24.095547030420462	18.461244614600663
12	20.68172516614371	27.66233006922138	31.802218556715644	19.853726207919266
13	25.917785888940077	24.66217796520075	27.171090400085053	22.248945745774122
14	20.7859269048781	23.604423146918553	28.396649998883618	27.212999949319734
15	22.598750322059107	30.89166161944964	24.53946876460784	21.97011929388341
16	21.97876497816043	31.24653164381125	25.82512326617627	20.94958011185205
17	20.18356881562642	30.256607247711592	28.666282297384882	20.893541639277107
18	20.2319566165717	30.11428087460005	29.292815106947177	20.36094740188107
19	20.967476174530532	28.837118655178706	29.615904552839478	20.57950061745128
20	22.256848798125134	28.502636619854055	28.780117739019634	20.460396843001174
21	22.753673307698957	28.98585951927751	27.672050546868242	20.588416626155283
22	21.697878537689885	28.4759630205768	29.286739354596385	20.539419087136928
23	21.86756973771772	28.95129610350402	28.40708641942061	20.77404773935765
24	21.515046500536123	28.11044754329904	28.830332395739656	21.544173560425183
25	22.170689614814155	28.366270933126774	28.2078072786882	21.25523217337087
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2203.0
1	2203.0
2	2428.0
3	2653.0
4	2653.0
5	2653.0
6	3726.0
7	4799.0
8	4799.0
9	4799.0
10	7962.5
11	11126.0
12	11126.0
13	11126.0
14	18686.5
15	26247.0
16	26247.0
17	26247.0
18	42306.5
19	58366.0
20	58366.0
21	58366.0
22	83103.5
23	107841.0
24	107841.0
25	107841.0
26	140304.0
27	172767.0
28	172767.0
29	172767.0
30	208150.5
31	243534.0
32	243534.0
33	243534.0
34	274083.0
35	304632.0
36	304632.0
37	304632.0
38	325495.5
39	346359.0
40	346359.0
41	346359.0
42	344033.5
43	341708.0
44	341708.0
45	341708.0
46	327322.0
47	312936.0
48	312936.0
49	312936.0
50	296689.0
51	280442.0
52	280442.0
53	280442.0
54	253406.0
55	226370.0
56	226370.0
57	226370.0
58	195567.5
59	164765.0
60	164765.0
61	164765.0
62	136526.0
63	108287.0
64	108287.0
65	108287.0
66	83905.5
67	59524.0
68	59524.0
69	59524.0
70	44039.5
71	28555.0
72	28555.0
73	28555.0
74	20439.5
75	12324.0
76	12324.0
77	12324.0
78	8552.0
79	4780.0
80	4780.0
81	4780.0
82	3262.0
83	1744.0
84	1744.0
85	1744.0
86	1114.0
87	484.0
88	484.0
89	484.0
90	295.0
91	106.0
92	106.0
93	106.0
94	67.0
95	28.0
96	28.0
97	28.0
98	19.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08283172547199558
2	0.01895422289457555
3	0.031106182619509034
4	0.03911301322544188
5	0.0481118405436142
6	0.016863944108070954
7	0.02203649839331961
8	0.0320273224237314
9	0.03192103706170574
10	0.024445633265901177
11	0.021540500037199876
12	0.017182800194147925
13	0.024445633265901177
14	0.03468445647437283
15	0.031991893969722844
16	0.022957638197541975
17	0.023063923559567632
18	0.02419763408784131
19	0.017891369274318978
20	0.01473823686755781
21	0.011974817454890721
22	0.017005657924105168
23	0.018281082268413053
24	0.016722230292036747
25	0.10685221728979413
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2822590.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.28789179572655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.93316041748461	74.00410173281693
2	7.544559741473725	12.416518313055692
3	1.2618027970690788	3.1149327609829647
4	0.4363421522731758	1.4362270324867814
5	0.22688408731047804	0.9334906613388397
6	0.13880983856880685	0.685342138579935
7	0.0913748909733733	0.5263332998884278
8	0.06979706706972816	0.4594762802154288
9	0.04650661014542329	0.34442378130893675
>10	0.22918435790327119	3.2998963822349126
>50	0.014538675348910686	0.8181876995770002
>100	0.0059601533637201175	0.8406934308544953
>500	6.906950501887496E-4	0.3655610154887474
>1k	3.8851596547231486E-4	0.7548154711709516
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	4653	0.16484859650179445	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3110	0.11018249196659805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	7.085690801710485E-5	0.0	0.0	0.0	0.0
10	7.085690801710485E-5	0.0	0.0	0.0	0.0
11	7.085690801710485E-5	3.5428454008552426E-5	0.0	0.0	0.0
12	7.085690801710485E-5	3.5428454008552426E-5	0.0	0.0	0.0
13	7.085690801710485E-5	3.5428454008552426E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGG	60	0.005871838	11.084812	5
GGTATCA	2525	0.0	10.619599	1
TAGACTG	315	0.0	10.255337	5
GTGGTAT	1190	0.0	10.227829	1
TGGTATC	1175	0.0	10.188232	2
TACCCCG	85	6.588406E-4	10.060166	5
TAGGACG	90	0.0011127472	9.501267	4
ACGGATC	80	0.004522975	9.498573	8
TAGGACC	145	1.7042785E-6	9.173637	4
TTAGGAC	250	1.8189894E-12	9.121055	3
GGCCTAG	220	1.6552804E-10	9.076471	1
GCACCGT	105	4.5010916E-4	9.04626	6
TTAGACT	305	0.0	9.033992	4
CCGTAAT	95	0.0018269125	8.998649	14
AGCTTCG	85	0.0073909545	8.94808	19
GCCTTAC	150	2.6700236E-6	8.874772	1
TGTCGCC	225	2.6193447E-10	8.86565	10
TAGACAG	405	0.0	8.680171	5
AGCACCG	100	0.0029024186	8.551141	5
CTAGCAC	200	2.5349436E-8	8.550989	3
>>END_MODULE