FastQCFastQC Report
Wed 25 May 2016
SRR1295289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822590
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105140.37249476544592025No Hit
GGTATCAACGCAGAGTACTTTTTTT89180.31595095284827057No Hit
TATCAACGCAGAGTACTTTTTTTTT62660.22199469281758952No Hit
GTGGTATCAACGCAGAGTACATGGG48400.17147371740139375No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42020.1488703637439373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC1352.7284841E-1112.6742083
GGACCGT658.048986E-411.6884926
GGTATCA32050.011.583171
TGGTATC12950.011.3040242
AGCGAGC600.00589196711.0797178
GTGGTAT13900.010.8886281
GTCGTGC700.001496123610.85368
CAGCGTC2300.010.7455232
GACTGCG909.552021E-510.5521117
AGCGTCA2400.010.2977943
TAATACG856.560499E-410.0648134
GCAGCGT2201.6370905E-119.518991
TGGACCG800.004495259.5056565
TAGGGCT2500.09.5056564
GTCTTAG2550.09.3323431
TACACTG5200.09.3228555
ACTGCGG950.00182984358.9970648
CCCGAGC850.0074578348.9382598
GTAACCC1606.687369E-78.9115523
CAGGACC3350.08.7962794
Produced by FastQC (version 0.11.5)