FastQCFastQC Report
Wed 25 May 2016
SRR1295288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647227
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG59680.16363116416938128No Hit
GTATCAACGCAGAGTACTTTTTTTT54640.1498124465518598No Hit
GGTATCAACGCAGAGTACTTTTTTT45870.125766781173752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1001.927494E-611.4020825
CTTACAC4600.010.1214143
GGTATCA36350.010.0173661
GTCTTAC3600.09.5073441
TATCGTG800.0045243989.4983477
CAGGACG950.00182165389.0016444
TGGACGG850.00742762778.9428095
GTCCTAA2607.2759576E-128.77601051
ATACCGT1304.3808024E-58.7677056
GTATTAG4800.08.7150661
TTACACC3850.08.6379414
GTGGTAT18450.08.3994431
TCGCCCA3750.08.3586612
TGGTATC17800.08.3270222
CTCGCTC2658.913048E-118.246793
TGTCGCC4150.08.23964310
CAAGACT6000.08.2348374
GTGTAAG5000.08.1763161
CTAGGAC3500.08.1443453
TCTGTCG3650.08.067098
Produced by FastQC (version 0.11.5)