FastQCFastQC Report
Wed 25 May 2016
SRR1295288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647227
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT139350.38207109127016226No Hit
GGTATCAACGCAGAGTACTTTTTTT119150.3266865484380325No Hit
TATCAACGCAGAGTACTTTTTTTTT84830.2325876618044339No Hit
GTGGTATCAACGCAGAGTACATGGG63710.17468065464529628No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56090.15378806967594832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2450.011.641033
GGTATCA49400.011.0981521
CCCTACG750.0026332610.1406322
AGGACCG750.0026332610.1406325
CAGCGTC2850.010.0072022
GCCTTAC2750.09.6912651
TAGGACA4150.09.3923024
GTCCTAA2700.09.1656811
AGCACCG1151.10269866E-49.0935015
TAGACAG5350.09.0625965
GTGGTAT21000.09.0196331
CGTAGGA950.00181277119.0064812
GGACCGT950.00183050498.996856
TACACTG8250.08.9882865
CTCGCCG850.0074609878.93792513
GCAGCGT3050.08.7380261
ACACCGT1201.7716156E-48.7052866
CTGTCGC2401.03682396E-108.7051669
GTCTTAG2850.08.6832761
CTAGGAC3200.08.6155753
Produced by FastQC (version 0.11.5)