FastQCFastQC Report
Wed 25 May 2016
SRR1295287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550354
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG51480.1449996253894682No Hit
GTATCAACGCAGAGTACTTTTTTTT48220.1358174424296845No Hit
ATTCCATTCCATTCCATTCCATTCC42270.11905855021780926No Hit
GGTATCAACGCAGAGTACTTTTTTT40850.11505894905127771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1502.1282176E-910.7690394
GGTATCA35250.09.4400251
GTCTTAT3700.09.2504961
GTATAGA4850.09.21340751
TTACACG950.00182100519.0019834
CCAGCGG950.00182685228.9988128
ACTGCGT850.00744674178.9399968
TAGACAG4200.08.5971325
CGCACTG1000.00290051528.5518845
CCAATAC3900.08.52743
GTGTAGG3600.08.451071
TAGACTG5300.08.4263855
GTCTTAA4200.08.1492461
GTCTTAC2803.274181E-118.1492451
GTCTAGA3300.08.0669311
GTCCAAC2252.1556843E-88.0285171
TTAGACT3350.07.94204764
CAGGACT4700.07.88471564
CGCAGAT1451.5654982E-47.8610318
GTGGTAT18450.07.83269931
Produced by FastQC (version 0.11.5)