FastQCFastQC Report
Wed 25 May 2016
SRR1295287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550354
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT122320.3445290244296766No Hit
GGTATCAACGCAGAGTACTTTTTTT102840.28966125631415907No Hit
TATCAACGCAGAGTACTTTTTTTTT73530.20710610829229986No Hit
GTGGTATCAACGCAGAGTACATGGG58550.1649131326059317No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46890.13207133711173588No Hit
GAATGGAATGGAATGGAATGGAATG38900.10956653899864632No Hit
ATTCCATTCCATTCCATTCCATTCC36910.10396146412442252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3650.011.9802423
GCAGCGT3450.011.5861541
CAGCGTC3900.011.2122772
TAGACAG4200.010.1850665
CATACCG750.002635023810.1397995
CGTCCTG750.00265636110.12994610
GGTATCA42700.010.074411
TCACGAC800.004494159.50606253
CTAGGAC3000.09.50606253
GCGTCAG4550.09.4015994
GTACTAG2451.8189894E-129.32296851
GTGGTAT20800.09.2426631
TAGGACT3850.09.1356954
TGGTATC20700.09.0009092
TCTAGAC3400.08.9468813
GCCCTAG2054.0145096E-98.8208171
CTACACT4450.08.7583934
ATAGGAC2401.0004442E-108.7138893
CTGCGCA1201.7712798E-48.7054229
CGTCAGA5250.08.5101885
Produced by FastQC (version 0.11.5)