FastQCFastQC Report
Wed 25 May 2016
SRR1295286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4451398
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG59320.1332615057112395No Hit
GTATCAACGCAGAGTACTTTTTTTT58010.13031861001869524No Hit
GGTATCAACGCAGAGTACTTTTTTT45940.10320353291258162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC3000.010.1364943
GGTATCA40000.09.27039051
GTCCTAG4650.09.201381
CGGATAG850.00741961748.9440655
TAGACTG5500.08.8119365
TTAGACT4700.08.6942984
CTAGGAC5250.08.6884233
CTTAGAC2850.08.669373
GTCTTAG4900.08.537881
TATACTG9250.08.4243425
GTGTTAG5100.08.3894941
AATACCG1252.7357263E-48.3627015
GTATTAG5750.08.2679071
GTACTAG3001.8189894E-128.2403481
TTAAGAC4850.08.229373
CTATACT7200.08.1831994
CTACACT7000.08.1454884
CTAGCAC3400.08.1054683
GTGGTAT20650.08.103751
GTGTAAG7400.08.09472751
Produced by FastQC (version 0.11.5)