##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295286_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4451398 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.772898985891622 31.0 30.0 33.0 25.0 34.0 2 29.76732949963135 31.0 30.0 33.0 25.0 34.0 3 30.150239767371957 31.0 30.0 34.0 26.0 34.0 4 33.53626254044235 35.0 33.0 37.0 27.0 37.0 5 33.728230322249324 35.0 35.0 37.0 28.0 37.0 6 33.8916913293307 35.0 35.0 37.0 28.0 37.0 7 33.801498540458525 35.0 35.0 37.0 28.0 37.0 8 33.809230268782976 35.0 35.0 37.0 28.0 37.0 9 34.81775231062242 38.0 34.0 39.0 27.0 39.0 10 34.71679773410511 38.0 34.0 39.0 27.0 39.0 11 35.110062501712946 38.0 35.0 39.0 27.0 39.0 12 35.114592314594205 38.0 35.0 39.0 27.0 39.0 13 35.013957862226654 38.0 35.0 39.0 27.0 39.0 14 35.150157546011386 38.0 33.0 40.0 24.0 41.0 15 35.7657989692227 39.0 34.0 41.0 27.0 41.0 16 35.86442012149891 39.0 34.0 41.0 27.0 41.0 17 35.67398826166521 39.0 34.0 41.0 27.0 41.0 18 35.65481114023055 39.0 34.0 41.0 27.0 41.0 19 35.71245954641665 39.0 34.0 41.0 26.0 41.0 20 35.684739940126676 39.0 34.0 41.0 26.0 41.0 21 35.67093191846696 39.0 34.0 41.0 26.0 41.0 22 35.40996581298729 39.0 34.0 40.0 25.0 41.0 23 35.086509900934495 39.0 33.0 40.0 24.0 41.0 24 35.40843011566254 39.0 34.0 40.0 25.0 41.0 25 33.982615573804004 38.0 32.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17543870415139295 1101 2 0.34084412815018084 1101 3 0.20457700838979775 1101 4 0.23113358038187926 1101 5 0.13072771381982307 1101 6 0.16122293963817214 1101 7 0.1793788888620611 1101 8 0.09583277304838589 1101 9 0.07412772892740804 1101 10 0.40543405168163815 1101 11 0.2766392431269509 1101 12 0.3148842620940968 1101 13 0.31879630696917616 1101 14 0.8182206904961262 1101 15 0.46683643841796396 1101 16 0.38906677925972843 1101 17 0.7359146686374345 1101 18 0.6404591867384042 1101 19 0.7199141949691281 1101 20 0.692065338059507 1101 21 0.749031342054117 1101 22 0.4769950157569127 1101 23 -1.7986332962811389 1101 24 -0.12079049319472546 1101 25 0.2508109173718793 1102 1 0.10317669886996228 1102 2 0.1828511571083986 1102 3 0.1310610543139319 1102 4 0.25765261811617535 1102 5 0.01023628547318367 1102 6 -0.017082476187702866 1102 7 0.010369084928768757 1102 8 0.08227427130648834 1102 9 0.2124988341865759 1102 10 0.7219375929839558 1102 11 0.41375029684844833 1102 12 0.38192361806665787 1102 13 0.3004869845629443 1102 14 0.766897630196361 1102 15 0.5145827345167149 1102 16 0.2849169715930415 1102 17 0.706791285230409 1102 18 0.601236319957458 1102 19 0.6655922961286151 1102 20 0.7406694819293733 1102 21 0.7174505942462304 1102 22 0.5667961195204967 1102 23 0.6177371433666679 1102 24 0.660179990599012 1102 25 0.510540741506901 1103 1 -0.4188310636504937 1103 2 -1.5984035020272493 1103 3 -0.3408666898699302 1103 4 -0.09091493217454172 1103 5 -0.23648623097503219 1103 6 -0.11495981322734394 1103 7 -0.11348517354539922 1103 8 0.1809688210074114 1103 9 0.7689033005923136 1103 10 0.7147876326222189 1103 11 0.43395088990656205 1103 12 0.22227438284757994 1103 13 -0.046583363843112124 1103 14 -1.1834106495029317 1103 15 -0.34009906385428224 1103 16 -0.22656283761864415 1103 17 0.18231193120833922 1103 18 0.4244601504781258 1103 19 0.4663468960272539 1103 20 0.4546613917239881 1103 21 0.6251958918922895 1103 22 0.17854936503111674 1103 23 0.8202005212435068 1103 24 0.651702669771602 1103 25 0.5805455944260416 1104 1 -0.12615963599554192 1104 2 -2.3968944449137553E-4 1104 3 -0.4324817994237691 1104 4 -0.2553290890118589 1104 5 -0.2211864629389524 1104 6 -0.061989339678710564 1104 7 -0.08808132694468185 1104 8 -0.07445144418728944 1104 9 -2.094360909796954 1104 10 -0.7383312720428634 1104 11 -0.6682902865306701 1104 12 -0.11673512686164855 1104 13 0.01793476148634454 1104 14 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1107 12 0.0059740471198992395 1107 13 0.10444274115369012 1107 14 -1.0458237532855819 1107 15 -0.5067979240774534 1107 16 -0.21211771661172918 1107 17 0.008095222192501694 1107 18 0.36808781864296947 1107 19 0.3506675714199261 1107 20 0.3717370651770793 1107 21 0.44695684784276324 1107 22 -0.2865702375493413 1107 23 0.633992710400328 1107 24 0.5147938844415236 1107 25 0.23729063632038105 1108 1 -0.1496137263806787 1108 2 0.22990490031231303 1108 3 -0.0784647521692321 1108 4 -0.2418194722600333 1108 5 -0.28366823832347166 1108 6 -0.2434104795445151 1108 7 -0.17796598031156208 1108 8 -0.2519950159938702 1108 9 0.2858084451239975 1108 10 0.2940857225805118 1108 11 0.12948098549753695 1108 12 0.009950440204640643 1108 13 -0.11459408263382187 1108 14 0.1699754274800256 1108 15 -0.09501386877568052 1108 16 -0.02946395195778706 1108 17 0.2656950562447733 1108 18 0.366955239869597 1108 19 0.3280286042533902 1108 20 0.39357118908567656 1108 21 0.3521890036036268 1108 22 0.3406792399289458 1108 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22 -0.04989825043656282 2211 23 0.5471073248027238 2211 24 -0.07025437122377554 2211 25 0.4585290811218954 2212 1 0.15389476168295957 2212 2 -1.0431758869535912 2212 3 -0.30011660957126196 2212 4 0.18871180126945575 2212 5 0.2216388512330738 2212 6 0.21446247001469487 2212 7 0.24624527717592315 2212 8 0.23462842579955634 2212 9 -2.100069816551269 2212 10 0.06984785329208165 2212 11 0.1393268358187285 2212 12 0.09875627902183481 2212 13 0.24008512972720553 2212 14 0.8022073334621638 2212 15 0.5211722909013972 2212 16 0.5272154954066153 2212 17 0.5047558936044751 2212 18 0.21109914908888072 2212 19 -0.4305769037395919 2212 20 0.431559057131814 2212 21 0.46260064711129445 2212 22 -1.5860450782497324 2212 23 -0.20074653863597547 2212 24 0.33283872088553323 2212 25 -0.35348854406740315 2213 1 0.24600164736026287 2213 2 0.5426832205080778 2213 3 0.2433901243999479 2213 4 0.4828806362756666 2213 5 0.3812859040173393 2213 6 0.2941686131849792 2213 7 0.3004255066275263 2213 8 0.2996879907317407 2213 9 0.6673484820671831 2213 10 0.7746500374968619 2213 11 0.21462260655919607 2213 12 0.2245519587512277 2213 13 0.2591101326393854 2213 14 -1.5486773077095464 2213 15 -0.29886868876700134 2213 16 -0.021490144891508578 2213 17 -2.1055210017499846 2213 18 -0.8085004559407878 2213 19 -0.519802791890541 2213 20 0.2474213237232945 2213 21 -0.11211528901827705 2213 22 0.491665435777648 2213 23 0.9270716284629472 2213 24 0.6667130883185379 2213 25 -0.7683298282459532 2214 1 0.4771048394084936 2214 2 0.3507267420592868 2214 3 0.3239631227556572 2214 4 0.6472566118028595 2214 5 0.5678928426589351 2214 6 0.5002927903283876 2214 7 0.49511609737739803 2214 8 0.3880728306362329 2214 9 0.8707681706950297 2214 10 0.8899194679122573 2214 11 0.28875193846887015 2214 12 0.1649137406541641 2214 13 0.2531383795447226 2214 14 1.191420591125798 2214 15 0.8317160301389279 2214 16 0.793024163472758 2214 17 0.7275610667564436 2214 18 0.6800238547182715 2214 19 -0.5966943426634685 2214 20 0.43490150025223073 2214 21 0.807204786311857 2214 22 0.9649404950916036 2214 23 1.4930084854210293 2214 24 1.2626529038621115 2214 25 1.2324352505832863 2215 1 -0.07611896556427666 2215 2 0.33458488614521187 2215 3 0.1933642059031584 2215 4 0.42543134139523886 2215 5 0.41532689050553273 2215 6 0.3604514699200081 2215 7 0.3318363904643675 2215 8 0.23336902748388155 2215 9 0.4201870787488531 2215 10 0.758526773848331 2215 11 0.4677653488664788 2215 12 0.30507295120044375 2215 13 0.11884788666710477 2215 14 1.0261305381820307 2215 15 0.5472759597495127 2215 16 0.4508494490505086 2215 17 0.720539494827257 2215 18 0.4829113378415002 2215 19 -0.2540495816689159 2215 20 -1.4424928776964663 2215 21 -1.808346041218897 2215 22 -0.17928006347269587 2215 23 0.6270981257371773 2215 24 0.7108613111498485 2215 25 -0.1538780278341676 2216 1 0.16427859672248601 2216 2 -0.21847657495387196 2216 3 -0.06366102167692134 2216 4 0.2738041787725578 2216 5 0.4266870346384266 2216 6 0.13449456956477235 2216 7 0.22868715890184177 2216 8 0.14456121707858216 2216 9 0.4655198436242358 2216 10 -1.552686213647604 2216 11 -0.4057322759182256 2216 12 -0.027093526057456074 2216 13 0.11746073576259874 2216 14 -0.3777691628872262 2216 15 -0.3483101789261269 2216 16 0.14523363350330243 2216 17 0.46748507575004794 2216 18 0.2498934206831649 2216 19 -1.1274812068023223 2216 20 -0.7110377566099828 2216 21 -0.6055346722761001 2216 22 -0.44163049193185344 2216 23 -4.1718760284982395 2216 24 -1.799496869865017 2216 25 -0.7640891255664641 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 19.0 4 47.0 5 61.0 6 118.0 7 376.0 8 213.0 9 529.0 10 1607.0 11 3853.0 12 7540.0 13 12639.0 14 18001.0 15 23066.0 16 27064.0 17 29997.0 18 31629.0 19 33127.0 20 33664.0 21 35355.0 22 37044.0 23 39688.0 24 43450.0 25 47841.0 26 53077.0 27 61067.0 28 71108.0 29 86317.0 30 105091.0 31 128742.0 32 167266.0 33 217592.0 34 288961.0 35 387987.0 36 531240.0 37 763307.0 38 1162711.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97758417177224 21.057973132482715 13.768219886459446 23.196222809285594 2 15.79408819886718 21.056275177014296 40.40819793245145 22.74143869166707 3 18.202130741094216 26.173072770096006 30.0654089740654 25.55938751474438 4 12.802983414934962 17.467917816752987 38.32501136040323 31.404087407908822 5 13.850532809618892 37.376776818713466 34.97151426174874 13.801176109918897 6 30.897557056172474 38.25526095949907 17.573934742487214 13.273247241841238 7 26.049666197868902 32.661736912554424 23.493171723563165 17.795425166013505 8 23.502640525178983 37.04495353938697 21.400760458666383 18.05164547676767 9 24.400879342551658 17.47048332377461 22.18971307651233 35.9389242571614 10 15.362110353781677 29.815290564406293 33.50381672013513 21.3187823616769 11 31.971646351465346 25.760668457981623 23.697712680451612 18.569972510101426 12 21.147540173253123 27.386859024337852 31.218164390493143 20.24743641191588 13 26.33195415894893 24.26629353381958 26.910641139694043 22.491111167537444 14 21.239095570626183 23.365976202633604 27.921025142216056 27.47390308452416 15 22.766531616849257 30.80347150245094 24.24060322052014 22.18939366017966 16 22.26979212379949 31.015530887516846 25.582787470398017 21.13188951828565 17 20.45012953004043 30.139412478071797 28.276377491141645 21.134080500746126 18 20.519523361270664 29.905154492762936 29.060540273554697 20.514781872411703 19 21.217387826526178 28.703094126090146 29.335081726757206 20.744436320626473 20 22.373605988883412 28.36368023877882 28.68893639260458 20.573777379733187 21 22.823285333362094 28.723457795344515 27.648064318703273 20.80519255259012 22 21.757120702199362 28.281481927543002 29.15716393354955 20.80423343670809 23 22.06519128287297 28.564344299958904 28.44247043536427 20.927993981803855 24 21.700266239568876 27.802902278686254 28.779713214438907 21.717118267305967 25 22.3347989979415 28.11393006596053 28.16066400881423 21.39060692728374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3715.0 1 3715.0 2 4053.0 3 4391.0 4 4391.0 5 4391.0 6 6307.5 7 8224.0 8 8224.0 9 8224.0 10 12762.5 11 17301.0 12 17301.0 13 17301.0 14 28635.0 15 39969.0 16 39969.0 17 39969.0 18 63427.0 19 86885.0 20 86885.0 21 86885.0 22 123707.5 23 160530.0 24 160530.0 25 160530.0 26 210918.5 27 261307.0 28 261307.0 29 261307.0 30 314637.5 31 367968.0 32 367968.0 33 367968.0 34 414517.0 35 461066.0 36 461066.0 37 461066.0 38 494454.0 39 527842.0 40 527842.0 41 527842.0 42 532182.5 43 536523.0 44 536523.0 45 536523.0 46 517655.0 47 498787.0 48 498787.0 49 498787.0 50 476363.0 51 453939.0 52 453939.0 53 453939.0 54 413997.5 55 374056.0 56 374056.0 57 374056.0 58 325329.5 59 276603.0 60 276603.0 61 276603.0 62 230497.5 63 184392.0 64 184392.0 65 184392.0 66 143716.5 67 103041.0 68 103041.0 69 103041.0 70 76737.0 71 50433.0 72 50433.0 73 50433.0 74 36142.5 75 21852.0 76 21852.0 77 21852.0 78 15118.5 79 8385.0 80 8385.0 81 8385.0 82 5722.5 83 3060.0 84 3060.0 85 3060.0 86 1966.5 87 873.0 88 873.0 89 873.0 90 534.0 91 195.0 92 195.0 93 195.0 94 117.0 95 39.0 96 39.0 97 39.0 98 30.5 99 22.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08195178233894161 2 0.02044301587950572 3 0.03138339910293351 4 0.03890912472890539 5 0.04838929253236848 6 0.017612444450035694 7 0.02262210658314534 8 0.03852722223445308 9 0.03841489797137888 10 0.024958451255088852 11 0.023273587308975743 12 0.01797188209187316 13 0.025857045359682508 14 0.04111068028515985 15 0.03778588209816332 16 0.024823662139399804 17 0.024239575971413924 18 0.029631140598975873 19 0.018780616786007454 20 0.01689356916636077 21 0.012625247169540894 22 0.0207575238161135 23 0.020106043090283097 24 0.020106043090283097 25 0.11122348529608002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4451398.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.45123981905846 #Duplication Level Percentage of deduplicated Percentage of total 1 87.62531191578103 68.74314359324737 2 9.01410233239507 14.143350076645195 3 1.7330854503686723 4.07888106881381 4 0.5801135986365025 1.8204252419571711 5 0.2869594174569358 1.1256161038625692 6 0.1673421457599234 0.7876919285308552 7 0.11379967466264916 0.6249407897803223 8 0.07666844142183248 0.48117874276300926 9 0.0604325577384597 0.4266908172017099 >10 0.3141329985956122 4.376227029525432 >50 0.018423270369586624 1.0017728374764985 >100 0.00865000856231497 1.1480663650637633 >500 5.466347364063513E-4 0.3054481285392167 >1k 3.7401304632844615E-4 0.6717463618741515 >5k 5.754046866582029E-5 0.2648209147188818 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 5932 0.1332615057112395 No Hit GTATCAACGCAGAGTACTTTTTTTT 5801 0.13031861001869524 No Hit GGTATCAACGCAGAGTACTTTTTTT 4594 0.10320353291258162 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.246485261484145E-5 0.0 4 0.0 0.0 0.0 2.246485261484145E-5 0.0 5 0.0 0.0 0.0 2.246485261484145E-5 0.0 6 0.0 0.0 0.0 2.246485261484145E-5 0.0 7 0.0 0.0 0.0 2.246485261484145E-5 0.0 8 0.0 0.0 0.0 2.246485261484145E-5 0.0 9 0.0 0.0 0.0 2.246485261484145E-5 0.0 10 0.0 0.0 0.0 2.246485261484145E-5 0.0 11 0.0 0.0 0.0 4.49297052296829E-5 0.0 12 0.0 0.0 0.0 4.49297052296829E-5 0.0 13 0.0 0.0 0.0 6.739455784452434E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGTAC 300 0.0 10.136494 3 GGTATCA 4000 0.0 9.2703905 1 GTCCTAG 465 0.0 9.20138 1 CGGATAG 85 0.0074196174 8.944065 5 TAGACTG 550 0.0 8.811936 5 TTAGACT 470 0.0 8.694298 4 CTAGGAC 525 0.0 8.688423 3 CTTAGAC 285 0.0 8.66937 3 GTCTTAG 490 0.0 8.53788 1 TATACTG 925 0.0 8.424342 5 GTGTTAG 510 0.0 8.389494 1 AATACCG 125 2.7357263E-4 8.362701 5 GTATTAG 575 0.0 8.267907 1 GTACTAG 300 1.8189894E-12 8.240348 1 TTAAGAC 485 0.0 8.22937 3 CTATACT 720 0.0 8.183199 4 CTACACT 700 0.0 8.145488 4 CTAGCAC 340 0.0 8.105468 3 GTGGTAT 2065 0.0 8.10375 1 GTGTAAG 740 0.0 8.0947275 1 >>END_MODULE