FastQCFastQC Report
Wed 25 May 2016
SRR1295286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4451398
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT148060.3326146078153425No Hit
GGTATCAACGCAGAGTACTTTTTTT124530.2797548096126206No Hit
TATCAACGCAGAGTACTTTTTTTTT89520.20110536060806067No Hit
GTGGTATCAACGCAGAGTACATGGG68770.15449079143226466No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58360.1311048798602147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG551.9511956E-413.8267185
AGCGTCA2450.013.1921153
GGACCGA701.09511355E-412.21029956
TACGTTG951.3523744E-511.0067965
GGTATCA52150.010.5137131
GTCTCGC2250.010.1535221
CGTGCGT750.00265666110.12991210
CGCGCAC750.00265666110.12991210
TCTACAC5900.09.3449623
TGGACCG1252.736623E-59.1256345
GGACCGT1252.767245E-59.1170246
GACCGTC1054.5118437E-49.0443617
GAACCGG850.00745924338.9382586
TCTCGCT3150.08.7516322
GTGGTAT24400.08.7386871
GTCCTAG4500.08.67281
CGTAGAC1451.6771706E-58.5226953
GTCTTAG4500.08.4612681
GAAGCGT1252.7549738E-48.3572726
TGGTATC23850.08.3302772
Produced by FastQC (version 0.11.5)