FastQCFastQC Report
Wed 25 May 2016
SRR1295285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190298
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43140.13522247764942336No Hit
GTGGTATCAACGCAGAGTACATGGG41210.12917288604387425No Hit
GGTATCAACGCAGAGTACTTTTTTT35410.1109927661930014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCG701.0882018E-412.218365
GCGCCAA600.005885326311.08146711
GTCTACG1105.997208E-610.3713241
AGCACCG1054.092866E-59.9557015
GTATTAG4700.09.7093251
TGAACCG1002.7476618E-49.5031685
GAACCGT800.00452334159.4985496
CCCCTAT1554.2186912E-79.2003681
GTCTAGG3000.09.19014551
GTATAGA4150.09.1634191
GGTATCA30300.09.130531
CTTACAC5100.09.1304963
TTAGACA3050.09.03584
GTTCTAG2950.09.0236381
GTATTGG3800.09.0066761
GTATTAA5200.08.7757361
AACAGCG1201.7676444E-48.7070057
TAGGACA4150.08.7016974
TAATACT5250.08.6886114
GACCCGC1107.158391E-48.6350456
Produced by FastQC (version 0.11.5)