FastQCFastQC Report
Wed 25 May 2016
SRR1295285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190298
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105130.32953034481418353No Hit
GGTATCAACGCAGAGTACTTTTTTT90010.28213665306501146No Hit
TATCAACGCAGAGTACTTTTTTTTT64900.20342927212442224No Hit
GTGGTATCAACGCAGAGTACATGGG46400.14544095880698293No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42910.13450154186223356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG604.0725444E-412.6746045
CAACCGT751.4845284E-512.6626856
AATATCG550.00305529412.0984865
TATACCG550.00305529412.0984865
ACGTCGC752.081187E-411.3964176
TACGTCG909.461077E-510.5621715
GGTATCA35850.010.1657991
TGCGAAA856.616087E-410.05566212
GTCTAGC1106.719771E-59.5155071
ATAGCGA900.00110782389.5059543
CTACGAC900.00110782389.5059543
TCTATAC3950.09.3856253
AGCGTCA2850.09.3391823
TAGGGCG950.00181418289.005645
GTGGTAT17900.08.8244361
TGGTATC17300.08.7366862
ATAGAAC3700.08.73523
TAGGACA3400.08.6671924
AGACCGT1107.16991E-48.6336496
GTCTACG1000.00286354648.5639561
Produced by FastQC (version 0.11.5)