FastQCFastQC Report
Wed 25 May 2016
SRR1295284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7905283
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA326340.41281254573681925No Hit
ATTCCATTCCATTCCATTCCATTCC245950.31112105664022405No Hit
GCTTATGTACTCTGCGTTGATACCA217450.275069216370875No Hit
GAATGGAATGGAATGGAATGGAATG182560.2309341740200825No Hit
CTTATGTACTCTGCGTTGATACCAC104560.13226597959870634No Hit
GTGGTATCAACGCAGAGTACATGGG90580.11458160321395199No Hit
GAGTACATAAGCAGTGGTATCAACG83880.10610625830852609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGC4600.010.3243129
TCTGTCG4650.09.804898
GCTTATG51350.09.6659311
TGTCGCC4450.09.6050910
CGTTCGT800.00452530529.49836710
GCGACTT1106.8589725E-59.49812713
TAGACAG12250.09.386045
GTCTTAG6900.09.2329251
TCTATAC11400.09.0855093
ACATAAG78850.09.0383093
TATTCCG8500.08.9434325
CATAAGC78800.08.815044
GTACATA89250.08.7574411
TATACTG19950.08.7164835
TCGGAAC2401.0186341E-108.70661613
CGTAGTA2304.0381565E-108.6758272
TAGAGCG1107.1622484E-48.6349347
CGACAAC1000.0029131448.54804415
GTCTAGG6700.08.5151071
TACATAA91450.08.3227762
Produced by FastQC (version 0.11.5)