##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295284_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7905283
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.197145124342796	31.0	30.0	33.0	26.0	34.0
2	30.177681305020958	31.0	30.0	34.0	26.0	34.0
3	30.511581305817895	31.0	30.0	34.0	26.0	34.0
4	33.92282287680277	35.0	35.0	37.0	28.0	37.0
5	34.07501198881811	35.0	35.0	37.0	30.0	37.0
6	34.314199377808485	36.0	35.0	37.0	30.0	37.0
7	34.284806628681096	37.0	35.0	37.0	30.0	37.0
8	34.36594553287972	37.0	35.0	37.0	30.0	37.0
9	35.367714856001996	39.0	35.0	39.0	28.0	39.0
10	35.28549098115779	39.0	35.0	39.0	28.0	39.0
11	35.67309139976393	39.0	35.0	39.0	30.0	39.0
12	35.66554606583977	39.0	35.0	39.0	30.0	39.0
13	35.57185251938482	39.0	35.0	39.0	29.0	39.0
14	35.79113385314606	39.0	35.0	41.0	26.0	41.0
15	36.398572448323485	39.0	36.0	41.0	27.0	41.0
16	36.48461389680799	39.0	36.0	41.0	28.0	41.0
17	36.29526216834995	39.0	36.0	41.0	27.0	41.0
18	36.273610065572605	39.0	36.0	41.0	27.0	41.0
19	36.34058477096898	39.0	36.0	41.0	27.0	41.0
20	36.3186943465528	39.0	36.0	41.0	27.0	41.0
21	36.3125665456885	39.0	36.0	41.0	27.0	41.0
22	36.04129529581673	39.0	35.0	41.0	27.0	41.0
23	35.721973394247875	39.0	34.0	41.0	26.0	41.0
24	36.05431620854054	39.0	34.0	41.0	27.0	41.0
25	34.76833593433657	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29968093691524444
1101	2	0.62630365141721
1101	3	0.3795562023765733
1101	4	0.398103639985095
1101	5	0.2626653081310337
1101	6	0.27645008484471845
1101	7	0.2685347547206476
1101	8	0.3290680844736116
1101	9	0.2015385787391324
1101	10	0.4930582259945311
1101	11	0.46261428233626134
1101	12	0.48228815827496163
1101	13	0.4661544561755875
1101	14	0.9664745648978936
1101	15	0.7324454055689174
1101	16	0.5534836282706053
1101	17	0.8095082554609334
1101	18	0.784695194587826
1101	19	0.7715372059738144
1101	20	0.8851605523505057
1101	21	0.8783690475629697
1101	22	0.6902860117314304
1101	23	-1.51664810503501
1101	24	0.11561082886012031
1101	25	0.504110193375773
1102	1	0.26644828913530816
1102	2	0.37418385531382015
1102	3	0.2786536354306861
1102	4	0.45423280925137277
1102	5	0.18166437022662763
1102	6	0.1851030159725724
1102	7	0.19793176253216416
1102	8	0.2897448073925659
1102	9	0.585036003938221
1102	10	0.9714815880443268
1102	11	0.6484661453569913
1102	12	0.5555238534245674
1102	13	0.5961859698851484
1102	14	1.1308891827948884
1102	15	0.8218976567574856
1102	16	0.6498062311515298
1102	17	0.8918217863331819
1102	18	0.9380583249503189
1102	19	0.8967068671645109
1102	20	1.0058590367877116
1102	21	0.9604769418220798
1102	22	0.8405909295256322
1102	23	0.8602652373505251
1102	24	0.8885174682491837
1102	25	0.7727785587414715
1103	1	-0.3719546296828753
1103	2	-1.6116596017020584
1103	3	-0.4590901510888905
1103	4	-0.026191323709241487
1103	5	-0.24401830697216553
1103	6	-0.08329385736460893
1103	7	-0.017513317270683615
1103	8	0.23151408485766467
1103	9	0.7999882021700415
1103	10	0.8187380138660245
1103	11	0.5471075359175472
1103	12	0.3742725887449154
1103	13	0.1678545226419672
1103	14	-0.9571300228488937
1103	15	-0.131412618811531
1103	16	-0.04399889951329783
1103	17	0.45162883056761416
1103	18	0.5949324365518933
1103	19	0.6022863215223779
1103	20	0.6052297571742216
1103	21	0.6845462000515496
1103	22	0.2979359391309515
1103	23	1.0802903935462709
1103	24	0.8799613639371557
1103	25	0.7371500660397601
1104	1	0.02100259312640773
1104	2	0.14358820334144795
1104	3	-0.2797554870835661
1104	4	-0.17718423983776432
1104	5	-0.1521955472628278
1104	6	-0.02982119484650525
1104	7	-0.015368195749019264
1104	8	0.10032469892365015
1104	9	-2.028314016921186
1104	10	-0.5208523478941203
1104	11	-0.3861233878558181
1104	12	0.049072270427302556
1104	13	0.19972372541289474
1104	14	-1.6831728391770255
1104	15	-0.4363529342590624
1104	16	-0.07676865412172873
1104	17	0.256605543807801
1104	18	0.4067478906111006
1104	19	0.402268448771153
1104	20	0.5509255551208128
1104	21	0.6696541540781382
1104	22	0.7513682901152521
1104	23	1.1036101592436367
1104	24	0.7845949725369508
1104	25	0.6489608236095918
1105	1	-0.24307921820663836
1105	2	-0.08109691057950741
1105	3	-1.4696154019737193
1105	4	-0.5822549950349654
1105	5	-0.4556750740683526
1105	6	-0.16746581032509766
1105	7	-0.17022597271235185
1105	8	-0.14883519764141795
1105	9	0.2610004212112855
1105	10	0.4483822743036683
1105	11	-0.16392763520376974
1105	12	-0.13808937720163783
1105	13	-0.2673067960376372
1105	14	0.5516134699468367
1105	15	0.21855798545669103
1105	16	-0.22665463135790276
1105	17	0.2691230035435126
1105	18	0.26334614361946507
1105	19	0.21597093080238494
1105	20	-0.29982312649097764
1105	21	0.08605449614643135
1105	22	0.16983285143303561
1105	23	0.39124011465258945
1105	24	0.2926380568527094
1105	25	0.5602902068686078
1106	1	-1.5990792464830008
1106	2	-0.973596096491324
1106	3	-1.1201997169718574
1106	4	-1.3462633931794485
1106	5	-0.9440283397872307
1106	6	-0.7406696842910208
1106	7	-0.5817882338142439
1106	8	-0.7035256222632142
1106	9	-0.29480718904146386
1106	10	-1.8060274094813948
1106	11	-1.2340921432184402
1106	12	-0.8596060862616213
1106	13	-1.0127054386874192
1106	14	-0.1992510732565549
1106	15	-0.8469543265312183
1106	16	-1.531332134074603
1106	17	-0.6763505159547165
1106	18	-0.642013390798823
1106	19	-0.5707884852666041
1106	20	-0.30967105485082413
1106	21	-0.32385232442853606
1106	22	-0.24459719100534727
1106	23	-0.7028470027908611
1106	24	-0.9363452848770066
1106	25	-1.6603132100194173
1107	1	0.08853952772434326
1107	2	0.24080445710580278
1107	3	0.04984837591337765
1107	4	0.23949037133617423
1107	5	0.03373548572116647
1107	6	0.10909093869272368
1107	7	0.03191814248508962
1107	8	-0.021076226340674964
1107	9	0.600282423666151
1107	10	0.07965095495635666
1107	11	0.11228667665890413
1107	12	-0.0015797137103277237
1107	13	0.21051862305178304
1107	14	-0.9863642807712267
1107	15	-0.438606398216713
1107	16	-0.151794877305079
1107	17	0.11593977201548
1107	18	0.4584804048522386
1107	19	0.3400667879783015
1107	20	0.4107158572837406
1107	21	0.43677534891240555
1107	22	-0.16752791234717535
1107	23	0.597640204658596
1107	24	0.4819478304652165
1107	25	0.13729809414468974
1108	1	-0.17238759812844506
1108	2	0.1191512153441181
1108	3	-0.11009585078132034
1108	4	-0.39217386914582875
1108	5	-0.3552837021639803
1108	6	-0.2698427803564769
1108	7	-0.24450393122402403
1108	8	-0.2370662279267961
1108	9	0.24697901542090506
1108	10	0.29830416916124847
1108	11	-0.03881092453510604
1108	12	-0.023704140670147922
1108	13	-0.21928738087463984
1108	14	0.06800134021182913
1108	15	-0.1584813378960419
1108	16	-0.1886373683971101
1108	17	0.1666297219907591
1108	18	0.1267066892186932
1108	19	0.13376287351931637
1108	20	0.27298078106522894
1108	21	0.24649382639643136
1108	22	0.2047496180461792
1108	23	0.031122720643757873
1108	24	0.09847972118835457
1108	25	-0.6449645033887776
1109	1	-0.8907474768481869
1109	2	-0.838367445940893
1109	3	-0.8449982188063103
1109	4	-0.6720589158714176
1109	5	-0.9991167576488351
1109	6	-1.2196816302487363
1109	7	-0.8771969363985974
1109	8	-1.3276222143954541
1109	9	-0.9861174128791106
1109	10	-1.1431985729338408
1109	11	-1.234077387660676
1109	12	-1.1761982730812264
1109	13	-1.1642463660561901
1109	14	-0.7771385932895001
1109	15	-1.3553628204408454
1109	16	-1.4681887004011145
1109	17	-1.4543505976113877
1109	18	-2.094816606024068
1109	19	-2.220216692755585
1109	20	-1.9070194209881137
1109	21	-1.6975189007527405
1109	22	-1.6667241489961668
1109	23	-1.3008085032124512
1109	24	-2.161169381513851
1109	25	-1.3380777161897512
1110	1	0.12268791680763158
1110	2	0.23634315590655675
1110	3	0.04062454471725374
1110	4	0.28668691090452114
1110	5	0.2505490447331695
1110	6	0.1769670819964233
1110	7	0.17687903566188368
1110	8	0.09240809218315604
1110	9	0.42291297142917017
1110	10	0.32823252620765686
1110	11	0.2930338570850779
1110	12	0.27280381982510704
1110	13	0.24115590097260764
1110	14	-2.3475544809943045
1110	15	-0.5946159941692528
1110	16	-0.124436327548068
1110	17	-0.14202056882143665
1110	18	-2.6319322487904913
1110	19	-0.3855389591689331
1110	20	-1.8570724380183066
1110	21	-0.19751777676972182
1110	22	-1.7182094900595715
1110	23	-0.4494344764318825
1110	24	0.1560649348766816
1110	25	0.12102358894935605
1111	1	-0.19150350383974057
1111	2	-1.2964266044696728
1111	3	-0.4281480641679778
1111	4	-0.005282361596997021
1111	5	-0.026297936273024902
1111	6	0.11519099908579022
1111	7	-0.04925667192019745
1111	8	-0.09531430064862434
1111	9	-1.69496267408784
1111	10	-0.3214422590722634
1111	11	-0.19996618993391735
1111	12	-0.19045147835017673
1111	13	-0.0837917289893042
1111	14	0.8371543334065166
1111	15	0.19782039217062675
1111	16	0.2782436148501546
1111	17	-0.12394629728837714
1111	18	0.05706663374810006
1111	19	-0.14029135152350847
1111	20	0.272498784999577
1111	21	-0.5456175017202938
1111	22	-0.25722824455678506
1111	23	-0.05915029689698059
1111	24	-0.5352578498268485
1111	25	-1.914918222587282
1112	1	0.25555006234430877
1112	2	0.42690683133881535
1112	3	0.09549215921367704
1112	4	0.4254938091321634
1112	5	0.3356812540651788
1112	6	0.25174981316417444
1112	7	0.24363563079896267
1112	8	0.11643535863625942
1112	9	-0.2098748672046611
1112	10	0.5526723548679513
1112	11	0.25500125176089483
1112	12	0.12772911694894873
1112	13	0.13505921032836454
1112	14	0.5764616137759049
1112	15	0.2846164982447519
1112	16	0.28971175392234016
1112	17	-3.5226353299988276
1112	18	-0.8375158061392156
1112	19	-1.0381133351725467
1112	20	-0.02592471893775894
1112	21	-0.4360193215608845
1112	22	-1.6657132344718448
1112	23	-1.333221463734887
1112	24	-0.26107357050167224
1112	25	0.1827205461197181
1113	1	-0.1814430652359107
1113	2	-0.06508150043024585
1113	3	-0.05442820947757809
1113	4	-0.4077468897828638
1113	5	-0.056576153753091774
1113	6	-0.14601456226063192
1113	7	-0.24549838992637518
1113	8	-0.20501519313073402
1113	9	0.26566461376756934
1113	10	0.44295862812820275
1113	11	-0.03061539226922605
1113	12	-0.07827252020486952
1113	13	-0.386778438549193
1113	14	-1.5271983928413988
1113	15	-1.7990635356434836
1113	16	-0.4848149691482746
1113	17	-0.30529622785879695
1113	18	-0.6422496105927493
1113	19	-0.16571139451528438
1113	20	0.25588253166658603
1113	21	-1.5520285842935166
1113	22	-2.0302276851429966
1113	23	-0.6995519152951459
1113	24	-2.4090106176901855
1113	25	-0.6590258978847103
1114	1	0.1637510819077299
1114	2	0.1847237706671656
1114	3	-0.06022960636753538
1114	4	0.3334926809007115
1114	5	0.15269996331667812
1114	6	0.17992034875960883
1114	7	0.0830159987158865
1114	8	0.012994557564780962
1114	9	0.3703600859936813
1114	10	0.2561814737840251
1114	11	0.18912146659560136
1114	12	0.2250548451308063
1114	13	0.18490437012158623
1114	14	0.9373474643528539
1114	15	0.400741608984589
1114	16	0.3589209526691093
1114	17	-0.3598041279917439
1114	18	-0.09486000319241583
1114	19	-0.5141928636409148
1114	20	-1.7651450619552733
1114	21	-0.7837492935660748
1114	22	-1.465980324941981
1114	23	-2.9218152814554017
1114	24	-1.9466049604276918
1114	25	-1.4413802772513478
1115	1	0.3666179807255645
1115	2	0.32591441242712804
1115	3	0.23982316732577402
1115	4	0.5066159191971593
1115	5	0.42605309673812997
1115	6	0.2965741427148316
1115	7	0.3996841694106976
1115	8	0.3687159248502212
1115	9	0.6069626456726382
1115	10	0.8648356074726635
1115	11	0.48990484407658386
1115	12	0.3727578402701397
1115	13	0.2985510367528619
1115	14	1.0053983677429343
1115	15	0.531061002631219
1115	16	0.5122419352772312
1115	17	0.528341254907275
1115	18	0.7562176772069122
1115	19	0.12598304572104269
1115	20	0.658959598939461
1115	21	-0.41535489669020365
1115	22	0.6570445764557036
1115	23	0.7239830832236436
1115	24	0.7608153393516588
1115	25	0.7808135158899319
1116	1	0.36206778844301724
1116	2	0.4381412460287528
1116	3	0.26765106183970744
1116	4	0.6070080265308562
1116	5	0.5452655416161676
1116	6	0.31740320471428873
1116	7	0.2907903597170858
1116	8	0.2416771653125238
1116	9	0.4585623820065621
1116	10	0.4426996400372971
1116	11	0.3068364524530267
1116	12	0.25776853775794706
1116	13	-0.1639767278470643
1116	14	0.8755377831879301
1116	15	0.4587751944978251
1116	16	0.4386433641974037
1116	17	0.5668324293682616
1116	18	0.5701387259980777
1116	19	0.6554198031281686
1116	20	0.4994437885701757
1116	21	-0.001931613565595569
1116	22	0.039010640382677764
1116	23	-0.875164364486082
1116	24	-0.32870309741377923
1116	25	-0.8949521667298441
1201	1	0.4043758775670696
1201	2	0.6411751707112359
1201	3	0.5420817293905067
1201	4	0.4176159912640216
1201	5	0.4101874378589727
1201	6	0.23730908638256665
1201	7	0.2750500482515008
1201	8	0.3798949265567728
1201	9	0.6021237563564128
1201	10	0.8605597496040431
1201	11	0.5410466609816282
1201	12	0.5279820871047178
1201	13	0.545608130691889
1201	14	1.1231070152566431
1201	15	0.7931536762462343
1201	16	0.5519300835301735
1201	17	0.6814552287686055
1201	18	0.505416867583051
1201	19	0.8770849346735332
1201	20	0.9057168571278496
1201	21	0.8530115186483371
1201	22	0.9465483961206402
1201	23	0.49295105059604083
1201	24	0.8812102790933309
1201	25	0.9791192045858423
1202	1	0.3870304262588249
1202	2	0.7553087348553227
1202	3	0.4969243826895209
1202	4	0.4498658909631956
1202	5	0.3013679520250818
1202	6	0.2269646278054296
1202	7	0.22029692098776366
1202	8	0.32985307740176495
1202	9	0.8853524283269252
1202	10	0.030332022689471216
1202	11	0.3763037968755967
1202	12	0.4316104924636406
1202	13	0.39751249211154516
1202	14	1.0820965944015128
1202	15	0.7822665620547298
1202	16	0.5672198321134161
1202	17	-1.422430711430387
1202	18	0.0995276228859936
1202	19	0.5417400511580936
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2210	22	1.1641753565283963
2210	23	1.4938831295943586
2210	24	1.171258234643659
2210	25	1.0475017947300387
2211	1	0.13256409320981533
2211	2	-0.5852097989736826
2211	3	-0.06271865224135453
2211	4	0.3272417037802029
2211	5	0.30654649763453534
2211	6	0.21113933166881793
2211	7	0.2574965510777858
2211	8	0.25609650184435395
2211	9	0.7230940172901796
2211	10	0.8113080532604187
2211	11	0.4157575295021019
2211	12	0.23932920950094427
2211	13	0.1643959423275021
2211	14	-0.8087910245795698
2211	15	-0.020223246498424885
2211	16	0.2157066654724673
2211	17	0.29622946143363293
2211	18	0.22007596687898712
2211	19	0.5537672684588628
2211	20	0.5770746030359319
2211	21	0.6406986419944687
2211	22	-0.04063541005003657
2211	23	0.5075375915210074
2211	24	-0.14637591646314974
2211	25	0.3806738687141973
2212	1	-0.004768227318823648
2212	2	-1.1183924846813582
2212	3	-0.40629340723562635
2212	4	0.09254454971188864
2212	5	0.15358674919485082
2212	6	0.17276754252315385
2212	7	0.08492838744759013
2212	8	0.05686593636055193
2212	9	-2.2451667205083368
2212	10	-0.12312352248191871
2212	11	-0.0058881296007129436
2212	12	-0.03697253981503934
2212	13	-0.01219232038356921
2212	14	0.6291938194205713
2212	15	0.28048865194103456
2212	16	0.32443991343880896
2212	17	0.3333915430726222
2212	18	0.11194562768489646
2212	19	-0.6739674191471181
2212	20	0.27232580795171657
2212	21	0.22211728290534438
2212	22	-1.8385731642900112
2212	23	-0.42283505624539686
2212	24	0.14560952376210423
2212	25	-0.37990511325440934
2213	1	0.21740178342630756
2213	2	0.46661867113228084
2213	3	0.1772529066219235
2213	4	0.36720467067029006
2213	5	0.3936440179416749
2213	6	0.26085088307395665
2213	7	0.24593369585394242
2213	8	0.24262435256961368
2213	9	0.6929927949070347
2213	10	0.6909924002789793
2213	11	0.08406602756343773
2213	12	0.07494192686801426
2213	13	0.10969547139210079
2213	14	-1.6068500266768666
2213	15	-0.33500084641863026
2213	16	-0.005582238714566756
2213	17	-2.05697774690843
2213	18	-0.8326362297180623
2213	19	-0.4465000354134716
2213	20	0.29852681940334236
2213	21	-0.21146565605749146
2213	22	0.37371157969834456
2213	23	0.8746012002920622
2213	24	0.6623128560979126
2213	25	-0.812512543641823
2214	1	0.29018754784937073
2214	2	0.19508266297101073
2214	3	0.13589024169950292
2214	4	0.4600556039557162
2214	5	0.5141363437285449
2214	6	0.39531703854336797
2214	7	0.3234110380422095
2214	8	0.24407119438422598
2214	9	0.6628927750739635
2214	10	0.628592794772743
2214	11	0.024734703077150755
2214	12	-0.014023169252631362
2214	13	0.10269311397513547
2214	14	0.9613933596317992
2214	15	0.5038026863337137
2214	16	0.518412439382459
2214	17	0.4421951706640712
2214	18	0.3651263935636493
2214	19	-0.8284731024935112
2214	20	0.16686654897938524
2214	21	0.4858179150653754
2214	22	0.6735412949813266
2214	23	1.246848739401031
2214	24	0.9096081899360655
2214	25	0.9466193469521897
2215	1	-0.027602634440157203
2215	2	0.3148953531638021
2215	3	0.268347300673895
2215	4	0.4969110050666359
2215	5	0.4779790624372282
2215	6	0.3547244167740189
2215	7	0.31530065155288867
2215	8	0.2303305973632419
2215	9	0.3738147835506922
2215	10	0.7425247371645582
2215	11	0.4067876608946861
2215	12	0.3629532252198331
2215	13	0.16015270283795502
2215	14	1.0175493919822287
2215	15	0.52304751020921
2215	16	0.5015527929621157
2215	17	0.7106551502805942
2215	18	0.5573106454405732
2215	19	-0.1419370393594832
2215	20	-1.4173007622470095
2215	21	-1.666612868480911
2215	22	-0.017701245933743337
2215	23	0.64916521829754
2215	24	0.7537491521871331
2215	25	0.13420533081184516
2216	1	0.060937458080911
2216	2	-0.2653676825442126
2216	3	-0.07263212699299615
2216	4	0.26011170817389484
2216	5	0.42085277452890324
2216	6	0.13056851820518744
2216	7	0.19307222887562148
2216	8	0.03962731504831396
2216	9	0.3530716239433289
2216	10	-1.4622556107974845
2216	11	-0.4027484515486819
2216	12	-0.14832877808401435
2216	13	0.04720567736956127
2216	14	-0.49876046351838
2216	15	-0.428377033228422
2216	16	0.029327656970430382
2216	17	0.37874851672887644
2216	18	0.22415952897029
2216	19	-1.2726338943781599
2216	20	-0.8610417709592184
2216	21	-0.7454212009186634
2216	22	-0.6744632914045496
2216	23	-4.587128578237028
2216	24	-2.067101639127344
2216	25	-1.0620434247979844
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6.0
3	28.0
4	61.0
5	133.0
6	180.0
7	598.0
8	317.0
9	817.0
10	2333.0
11	5194.0
12	10128.0
13	16519.0
14	23919.0
15	30494.0
16	36346.0
17	40963.0
18	44023.0
19	46623.0
20	48653.0
21	50627.0
22	53525.0
23	57569.0
24	63545.0
25	70409.0
26	79657.0
27	92405.0
28	108418.0
29	134049.0
30	164071.0
31	203053.0
32	267966.0
33	358096.0
34	485279.0
35	671622.0
36	952586.0
37	1425100.0
38	2359971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.52656052122265	21.94437055707811	13.424380011847582	22.10468890985166
2	14.533744059915103	21.70430697354169	42.25423303997347	21.50771592656974
3	17.396786744485855	28.448110053647053	30.666561724480196	23.4885414773869
4	11.979789975395512	16.838768158353535	39.43874231750406	31.742699548746888
5	13.910299602052662	37.174943441462354	34.69790982728954	14.216847129195443
6	30.585385331484797	38.69477709926794	18.298763575855126	12.421073993392142
7	24.503905418697272	33.183659567231985	25.857839571012526	16.454595443058217
8	21.98347866702082	38.8879109848933	22.546700750874198	16.581909597211677
9	23.867400756074293	17.158804200952673	23.072692618114147	35.901102424858884
10	15.322525442457021	29.177547500620932	33.11827040005324	22.381656656868802
11	32.05767046982201	27.09740274118194	23.127705675632075	17.717221113363973
12	20.387987030239664	27.62859308869873	32.84168724294414	19.141732638117475
13	25.114546624132707	25.273606119545526	28.5090642782057	21.102782978116068
14	20.990029797853698	22.074885788814242	28.814778885043708	28.12030552828835
15	23.237975854143905	29.92783019596142	24.209671553490715	22.62452239640396
16	22.92782821646537	31.56700852224381	25.408205419542263	20.096957841748555
17	19.517768472435822	30.913010938873015	29.872151012245894	19.697069576445266
18	19.61177382364571	30.855541320680114	30.464670620798735	19.068014234875445
19	20.603590393060614	27.96651513752124	29.879628587008643	21.5502658824095
20	22.288034819428365	28.497273501210067	28.436330392367598	20.778361286993967
21	23.181479127405517	30.08995315988592	26.82160778590384	19.90695992680473
22	20.51328248072404	29.16950761517592	30.035589403874642	20.2816205002254
23	21.11705556557193	29.644958548026406	28.991190862536335	20.24679502386533
24	20.54323045738431	27.233222639616862	29.18948641227988	23.034060490718947
25	22.469917334167796	28.254727321287394	27.733571785239796	21.541783559305014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4462.0
1	4462.0
2	5162.5
3	5863.0
4	5863.0
5	5863.0
6	8810.5
7	11758.0
8	11758.0
9	11758.0
10	18833.0
11	25908.0
12	25908.0
13	25908.0
14	45830.5
15	65753.0
16	65753.0
17	65753.0
18	105564.5
19	145376.0
20	145376.0
21	145376.0
22	210452.5
23	275529.0
24	275529.0
25	275529.0
26	365887.0
27	456245.0
28	456245.0
29	456245.0
30	559238.0
31	662231.0
32	662231.0
33	662231.0
34	774358.5
35	886486.0
36	886486.0
37	886486.0
38	998381.5
39	1110277.0
40	1110277.0
41	1110277.0
42	1133171.5
43	1156066.0
44	1156066.0
45	1156066.0
46	1038037.5
47	920009.0
48	920009.0
49	920009.0
50	831677.0
51	743345.0
52	743345.0
53	743345.0
54	654128.5
55	564912.0
56	564912.0
57	564912.0
58	478904.0
59	392896.0
60	392896.0
61	392896.0
62	321182.0
63	249468.0
64	249468.0
65	249468.0
66	190111.5
67	130755.0
68	130755.0
69	130755.0
70	95844.5
71	60934.0
72	60934.0
73	60934.0
74	42765.5
75	24597.0
76	24597.0
77	24597.0
78	16664.5
79	8732.0
80	8732.0
81	8732.0
82	5757.0
83	2782.0
84	2782.0
85	2782.0
86	1719.5
87	657.0
88	657.0
89	657.0
90	425.0
91	193.0
92	193.0
93	193.0
94	114.0
95	35.0
96	35.0
97	35.0
98	24.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08379206664707639
2	0.019809537495368602
3	0.03186476689069828
4	0.038784190268710175
5	0.04724688540562052
6	0.01721633494967859
7	0.020859468282160173
8	0.03660843008403368
9	0.03649458216739363
10	0.023528569438943554
11	0.022238293050356326
12	0.01719103541264747
13	0.02467969837385961
14	0.03840469721324334
15	0.035533199760210986
16	0.023376772216756818
17	0.022997279161289987
18	0.027298200456580744
19	0.01787412291248776
20	0.015053224533517647
21	0.011979330784236315
22	0.019303546754746163
23	0.019708339347244114
24	0.018873454625217084
25	0.11121676478881276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	7905283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.93697687437921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.43212213089896	28.09457514775965
2	14.733792654577954	12.652490289630238
3	6.996833449726227	9.012686280743337
4	3.882003146979512	6.667259173925066
5	2.4339013000041785	5.225218191640046
6	1.6174751521783504	4.16696959223789
7	1.0820839763008259	3.2522990266595406
8	0.7676894572821436	2.6369811579222504
9	0.5898001109296234	2.2791810351142376
>10	2.3562508193788876	16.148385158150997
>50	0.06489407417379578	1.9136662726873717
>100	0.03676667281933154	3.020572457827997
>500	0.003951333339713073	1.190330262565178
>1k	0.002108978306215742	1.8115094099550273
>5k	1.7822351151958386E-4	0.5544531054168933
>10k+	1.4851959293298654E-4	1.3734234377642116
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	32634	0.41281254573681925	No Hit
ATTCCATTCCATTCCATTCCATTCC	24595	0.31112105664022405	No Hit
GCTTATGTACTCTGCGTTGATACCA	21745	0.275069216370875	No Hit
GAATGGAATGGAATGGAATGGAATG	18256	0.2309341740200825	No Hit
CTTATGTACTCTGCGTTGATACCAC	10456	0.13226597959870634	No Hit
GTGGTATCAACGCAGAGTACATGGG	9058	0.11458160321395199	No Hit
GAGTACATAAGCAGTGGTATCAACG	8388	0.10610625830852609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.05990740622442E-5	0.0	0.0	0.0
3	0.0	5.05990740622442E-5	0.0	0.0	0.0
4	0.0	5.05990740622442E-5	0.0	0.0	0.0
5	0.0	1.3914745367117154E-4	0.0	0.0	0.0
6	2.52995370311221E-5	1.3914745367117154E-4	0.0	0.0	0.0
7	2.52995370311221E-5	1.3914745367117154E-4	0.0	0.0	0.0
8	2.52995370311221E-5	1.3914745367117154E-4	0.0	0.0	0.0
9	2.52995370311221E-5	1.3914745367117154E-4	0.0	1.264976851556105E-5	0.0
10	2.52995370311221E-5	1.8974652773341575E-4	0.0	1.264976851556105E-5	0.0
11	2.52995370311221E-5	2.023962962489768E-4	0.0	2.52995370311221E-5	0.0
12	2.52995370311221E-5	2.023962962489768E-4	0.0	2.52995370311221E-5	0.0
13	2.52995370311221E-5	2.276958332800989E-4	0.0	5.05990740622442E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGC	460	0.0	10.324312	9
TCTGTCG	465	0.0	9.80489	8
GCTTATG	5135	0.0	9.665931	1
TGTCGCC	445	0.0	9.60509	10
CGTTCGT	80	0.0045253052	9.498367	10
GCGACTT	110	6.8589725E-5	9.498127	13
TAGACAG	1225	0.0	9.38604	5
GTCTTAG	690	0.0	9.232925	1
TCTATAC	1140	0.0	9.085509	3
ACATAAG	7885	0.0	9.038309	3
TATTCCG	850	0.0	8.943432	5
CATAAGC	7880	0.0	8.81504	4
GTACATA	8925	0.0	8.757441	1
TATACTG	1995	0.0	8.716483	5
TCGGAAC	240	1.0186341E-10	8.706616	13
CGTAGTA	230	4.0381565E-10	8.675827	2
TAGAGCG	110	7.1622484E-4	8.634934	7
CGACAAC	100	0.002913144	8.548044	15
GTCTAGG	670	0.0	8.515107	1
TACATAA	9145	0.0	8.322776	2
>>END_MODULE