FastQCFastQC Report
Wed 25 May 2016
SRR1295284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7905283
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA342170.43283712929695245No Hit
GCTTATGTACTCTGCGTTGATACCA241560.30556780826189267No Hit
ATTCCATTCCATTCCATTCCATTCC219740.2779660133609385No Hit
GAATGGAATGGAATGGAATGGAATG218980.2770046309537559No Hit
GTATCAACGCAGAGTACTTTTTTTT197120.2493522369787394No Hit
GGTATCAACGCAGAGTACTTTTTTT148680.18807675828936168No Hit
CTTATGTACTCTGCGTTGATACCAC124940.15804620783341974No Hit
TATCAACGCAGAGTACTTTTTTTTT118260.14959616246502497No Hit
GTGGTATCAACGCAGAGTACATGGG99860.12632058839639265No Hit
GAGTACATAAGCAGTGGTATCAACG93380.11812353839830908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG1001.0004442E-813.3085795
TAGGCGA909.462765E-510.5624324
GCTTATG50800.010.005651
GGACGGT900.00111793859.4968696
ACCGTCC1106.870818E-59.4966298
TAGACTG10850.09.3747075
TCGGAAC2650.09.31744813
GTATTAG11300.09.1815451
ACATAAG81950.09.152393
TATTCCG6850.09.0204145
TCGCGCG850.0074620548.9380049
CTTAGAC6300.08.9026223
CCGTGCG1401.0914555E-58.8182989
CATAAGC84250.08.7558494
GTACATA95700.08.643221
CCAGGAC10600.08.6093793
TACATAA93750.08.5986642
CTAATAC11000.08.555573
TCGGCGT1000.00291647138.54696711
AACGATT3450.08.53320217
Produced by FastQC (version 0.11.5)