FastQCFastQC Report
Wed 25 May 2016
SRR1295283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718129
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA33380.12280506186424558No Hit
GTGGTATCAACGCAGAGTACATGGG28680.10551375596963941No Hit
GTATCAACGCAGAGTACTTTTTTTT28330.10422610553067937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGTA855.3168398E-511.1783652
TAGACTG2950.010.6293125
TATTCCG951.6414285E-410.0020655
CTAATAC3600.09.7655443
GTATTAG4300.09.5091381
GTCTAGG2401.8189894E-129.5091381
TACACCG800.0045096039.5019615
AGGACGG900.00111197779.501965
ACTGCGT800.0045219459.4988138
ACACCGT900.00111535259.4988136
GTGTAGA4250.09.1735211
TCTATAC3450.09.0884973
TGATCCG1901.05483196E-89.0018585
GTCCTAT2252.564775E-108.87519551
ACTGATC3150.08.7449398
CTAGACT2950.08.696714
GTCTTAA4100.08.5814171
CCAGGAC5550.08.560013
TATACTG8000.08.5517655
CTAAGAC2901.8189894E-128.5186853
Produced by FastQC (version 0.11.5)