FastQCFastQC Report
Wed 25 May 2016
SRR1295283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718129
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67910.24984097517078843No Hit
GGTATCAACGCAGAGTACTTTTTTT57510.21157936212740455No Hit
TATCAACGCAGAGTACTTTTTTTTT44550.16389950587334157No Hit
GTACATAAGCAGTGGTATCAACGCA35900.13207614502475784No Hit
GTGGTATCAACGCAGAGTACATGGG32700.12030334101140894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC759.71193E-713.9293148
ACGGATC604.1037565E-412.6630128
GTATTAG4550.010.8774211
CAGCGCT700.001495620510.8540119
AGCGTCA2250.010.5602813
TAGGACA4000.010.4546784
GCAGCGT2250.010.1522591
AAGGGCG750.002638847310.137875
CAGCGTC2350.010.1109072
CTAGGAC3250.09.942913
TAGACAG4900.09.892185
TAAGACA4050.09.6215894
GTATTAT5150.09.4253391
CTAAGAC2850.09.3375113
CTACACT4100.09.2724414
GTCCTAT1856.491973E-99.2605081
CTTACAC4650.09.1976643
GTTCTAG3650.09.1266031
CAGCGTT850.0074553718.9385979
CAATACT3300.08.9282384
Produced by FastQC (version 0.11.5)