FastQCFastQC Report
Wed 25 May 2016
SRR1295282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2878613
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1542005.356746460882376No Hit
GCTTATGTACTCTGCGTTGATACCA919613.194628802134917No Hit
CTTATGTACTCTGCGTTGATACCAC577452.006000806638475No Hit
GAGTACATAAGCAGTGGTATCAACG391251.359161512853586No Hit
TATGTACTCTGCGTTGATACCACTG366001.2714456580304474No Hit
GCGTTGATACCACTGCTTATGTACT297491.0334490951023985No Hit
CCCATGTACTCTGCGTTGATACCAC273870.9513956895213076No Hit
CATAAGCAGTGGTATCAACGCAGAG247340.8592332487903028No Hit
CTGCTTATGTACTCTGCGTTGATAC212530.7383069554677896No Hit
ACGCAGAGTACATAAGCAGTGGTAT203020.705270211730441No Hit
ACTCTGCGTTGATACCACTGCTTAT198540.6897071610529099No Hit
GTATCAACGCAGAGTACATAAGCAG193820.6733103755176538No Hit
CATGTACTCTGCGTTGATACCACTG170910.5937234355573326No Hit
TATCAACGCAGAGTACATAAGCAGT159760.5549895036255308No Hit
GTACATGATAAGCAGTGGTATCAAC147090.510975250928138No Hit
GGTATCAACGCAGAGTACATAAGCA146780.5098983434035766No Hit
ATCATGTACTCTGCGTTGATACCAC138400.48078710128801616No Hit
GTACATGGGAAGCAGTGGTATCAAC129950.45143268650561924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA127070.4414278682129206No Hit
GTATCAACGCAGAGTACTTTTTTTT115530.4013391171373158No Hit
GTGGTATCAACGCAGAGTACATAAG114030.3961282742765353No Hit
AAGCAGTGGTATCAACGCAGAGTAC112470.3907089977013235No Hit
GTACTGGTTCACTATCGGTCAGTCA98400.3418312916672022No Hit
ACATAAGCAGTGGTATCAACGCAGA85390.2966359145880325No Hit
GCAGTGGTATCAACGCAGAGTACAT83950.2916335054416832No Hit
GTGGTATCAACGCAGAGTACATGGG81470.28301824524519276No Hit
GTACTCTGCGTTGATACCACTGCTT81210.28211503248265746No Hit
CCACTGCTTATGTACTCTGCGTTGA78090.271276479332234No Hit
GTTGATACCACTGCTTATGTACTCT76300.2650582068517025No Hit
TATCAACGCAGAGTACTTTTTTTTT75470.2621748738020706No Hit
GGTATCAACGCAGAGTACTTTTTTT75390.26189696218282904No Hit
GATACCACTGCTTATGTACTCTGCG75190.261202183134725No Hit
GGATACCACGTGTCCCGCCCTACTC70380.2444927470278221No Hit
ATACCACTGCTTATGTACTCTGCGT68740.2387955588333687No Hit
ATACAGGGTGACAGCCCCGTACACA62030.21548572176947717No Hit
CAGTGGTATCAACGCAGAGTACATA56890.1976299002332026No Hit
GAGTACATGGGAAGCAGTGGTATCA55320.19217588470558564No Hit
TGATACCACTGCTTATGTACTCTGC54230.18838933889341847No Hit
TACCACTGCTTATGTACTCTGCGTT54180.18821564413139244No Hit
GTACATGGGATAAGCAGTGGTATCA54020.1876598208929092No Hit
GAGTACATGATAAGCAGTGGTATCA52610.18276162860377548No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51910.18032990193541124No Hit
ATCATTAACTGAATCCATAGGTTAA49820.17306946088272374No Hit
ACCCTGTATCGCGCGCCTTTCCAGA48840.16966504354701378No Hit
GCAGAGTACATAAGCAGTGGTATCA47410.1646973733530697No Hit
GCTTATCCCATGTACTCTGCGTTGA46480.16146665077938577No Hit
GTTAATGATAGTGTGTCGAAACACA45370.1576106270624082No Hit
GCGTTGATACCACTGCTTCCCATGT43210.15010701334288423No Hit
GCTTATCATGTACTCTGCGTTGATA41960.1457646442922338No Hit
CCCCATGTACTCTGCGTTGATACCA41220.14319396181424873No Hit
GTATCAACGCAGAGTACATGGGAAG40560.14090119095550532No Hit
GAACAAAAAAAAAAAAAAAAAAAAA40140.13944215495448675No Hit
GTATTTAGCCTTGGAGGATGGTCCC39320.13659356085726007No Hit
GCGTTGATACCACTGCTTATCATGT39200.13617669342839764No Hit
CCCATATTCAGACAGGATACCACGT38140.13249436447344606No Hit
ATCAACGCAGAGTACATAAGCAGTG37830.13141745694888476No Hit
GATAAGCAGTGGTATCAACGCAGAG36870.12808251751798522No Hit
GTTCACTATCGGTCAGTCAGGAGTA36850.12801303961317484No Hit
ACGCAGAGTACTTTTTTTTTTTTTT36780.12776986694633838No Hit
GGTATCAACGCAGAGTACATGGGGG35620.12374014846733479No Hit
TATCAACGCAGAGTACATGGGAAGC35510.12335801999087755No Hit
TATTCAGACAGGATACCACGTGTCC35260.12248954618074746No Hit
GTACACAAGCAGTGGTATCAACGCA34630.12030099217921965No Hit
CTATCGGTCAGTCAGGAGTATTTAG34250.11898091198782192No Hit
GGTTAATGAGGCGAACCGGGGGAAC34170.1187030003685803No Hit
GGTATCAACGCAGAGTACATGGGAA34130.11856404455895947No Hit
ACGCAGAGTACATGGGAAGCAGTGG33410.11606283998578482No Hit
GTACATAAGCAGTTGTATCAACGCA33390.11599336208097441No Hit
GTATCAACGCAGAGTACATGGGGGT32110.11154677617310838No Hit
TCTAAGTACCCCGAGGAAAAGAAAT31700.11012247912449502No Hit
GCCCAGAGCCTGAATCAGTGTGTGT31690.11008774017208982No Hit
GGATAAGCAGTGGTATCAACGCAGA31300.1087329210282869No Hit
CAGTGGTATCAACGCAGAGTACATG31260.10859396521866607No Hit
GCCTTGGAGGATGGTCCCCCCATAT31220.10845500940904526No Hit
GTCAGGAGTATTTAGCCTTGGAGGA31040.1078297082657516No Hit
ACGCAGAGTACATGATAAGCAGTGG30620.10637067226473304No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG29740.10331364445307514No Hit
ACTCTGCGTTGATACCACTGCTTCC29700.10317468864345432No Hit
GTACATGGGGGTTAAGCGACTAAGC29550.10265360435737629No Hit
TATCAACGCAGAGTACATGATAAGC29070.10098613464192652No Hit
GTACATGGTAAGCAGTGGTATCAAC28960.10060400616546927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG250.006025941619.0040153
TAGCGAG250.006025941619.0040155
GTTTTCG350.002174547216.28151516
TTCGTTG4100.015.75367513
TCGTTGA4000.015.67259114
CTAGGGC551.1267164E-515.5487394
CGAGTCT508.728194E-515.1979279
TAGAACC953.0377123E-1015.003174
CAGTACG456.772688E-414.77550814
AGTTGTA4800.014.44569711
CTTCGTT4350.014.41157912
TCTTCGT4650.014.29887811
CAGTTGT5000.014.058084510
CCTTATA1700.013.9732982
TAACTCG759.657906E-713.9362785
GGTATAG1101.8371793E-1013.827111
TCTATAC1101.8553692E-1013.8211023
CCTAGCT551.9578422E-413.8208612
AGTTGAC551.9633339E-413.816298510
GTTGTAT4950.013.81605812
Produced by FastQC (version 0.11.5)