FastQCFastQC Report
Wed 25 May 2016
SRR1295282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2878613
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1647465.723103452947653No Hit
GCTTATGTACTCTGCGTTGATACCA1032153.5855809725030765No Hit
CTTATGTACTCTGCGTTGATACCAC669892.3271276826721756No Hit
GAGTACATAAGCAGTGGTATCAACG427961.486688207133088No Hit
GCGTTGATACCACTGCTTATGTACT336961.1705637402457363No Hit
TATGTACTCTGCGTTGATACCACTG331471.1514920553752797No Hit
CCCATGTACTCTGCGTTGATACCAC323101.1224155522121244No Hit
CATAAGCAGTGGTATCAACGCAGAG309491.0751358379886424No Hit
GTATCAACGCAGAGTACTTTTTTTT297531.033588050912019No Hit
CTGCTTATGTACTCTGCGTTGATAC246840.8574963011700427No Hit
ACGCAGAGTACATAAGCAGTGGTAT241190.8378687930611026No Hit
ACTCTGCGTTGATACCACTGCTTAT231430.8039635755136242No Hit
GTATCAACGCAGAGTACATAAGCAG215540.7487633801417558No Hit
CATGTACTCTGCGTTGATACCACTG194490.6756378853288025No Hit
GGTATCAACGCAGAGTACTTTTTTT194180.6745609778042412No Hit
TATCAACGCAGAGTACTTTTTTTTT186850.649097325691227No Hit
TATCAACGCAGAGTACATAAGCAGT174980.6078621891862505No Hit
GTACTTTTTTTTTTTTTTTTTTTTT166290.5776740395461287No Hit
ATCATGTACTCTGCGTTGATACCAC156010.5419623964735795No Hit
GGTATCAACGCAGAGTACATAAGCA155760.5410939226634495No Hit
GTACATGATAAGCAGTGGTATCAAC146470.5088214358790154No Hit
GTACATGGGAAGCAGTGGTATCAAC133060.4622365007036375No Hit
AAGCAGTGGTATCAACGCAGAGTAC131470.4567130072712101No Hit
GTGGTATCAACGCAGAGTACATAAG121400.42173088219917026No Hit
ACGCAGAGTACTTTTTTTTTTTTTT116500.40470879552062056No Hit
ACATAAGCAGTGGTATCAACGCAGA110900.3852549821737066No Hit
GTACTGGTTCACTATCGGTCAGTCA97130.3374194447117414No Hit
GCAGTGGTATCAACGCAGAGTACAT95100.33036743737348506No Hit
CCACTGCTTATGTACTCTGCGTTGA91340.31730559126912855No Hit
GATACCACTGCTTATGTACTCTGCG87230.30302788183058993No Hit
GTGGTATCAACGCAGAGTACATGGG84260.2927104129662445No Hit
GTACTCTGCGTTGATACCACTGCTT82550.2867700521049547No Hit
ATACCACTGCTTATGTACTCTGCGT77960.2708248729509663No Hit
GGATACCACGTGTCCCGCCCTACTC76210.2647455562800557No Hit
GTTGATACCACTGCTTATGTACTCT73850.2565471635124277No Hit
ATACAGGGTGACAGCCCCGTACACA71560.2485919434116361No Hit
CAGTGGTATCAACGCAGAGTACATA69820.24254736569313068No Hit
GAGTACTTTTTTTTTTTTTTTTTTT66010.22931182482674814No Hit
TACCACTGCTTATGTACTCTGCGTT61240.21274134452946608No Hit
GAGTACATGGGAAGCAGTGGTATCA59670.20728732900184918No Hit
ACCCTGTATCGCGCGCCTTTCCAGA58750.20409134538057044No Hit
TGATACCACTGCTTATGTACTCTGC58050.2016596187122062No Hit
GAGTACATGATAAGCAGTGGTATCA57180.1986373298529535No Hit
GCAGAGTACATAAGCAGTGGTATCA56320.195649779946106No Hit
GTACATGGGATAAGCAGTGGTATCA56210.19526765146964875No Hit
ATCATTAACTGAATCCATAGGTTAA53750.1867218691779687No Hit
CCCCATGTACTCTGCGTTGATACCA51040.17730761307615855No Hit
GCTTATCCCATGTACTCTGCGTTGA50660.17598753288476082No Hit
GCTTATCATGTACTCTGCGTTGATA48960.17008191097587624No Hit
GCGTTGATACCACTGCTTCCCATGT48050.16692066630700272No Hit
CCCATATTCAGACAGGATACCACGT46080.16007709268317763No Hit
GTTAATGATAGTGTGTCGAAACACA45770.1590001851586163No Hit
ATCAACGCAGAGTACATAAGCAGTG44180.15347669172618897No Hit
GTATCAACGCAGAGTACATGGGAAG44010.1528861295353005No Hit
GCAGAGTACTTTTTTTTTTTTTTTT43560.15132287667706637No Hit
GCGTTGATACCACTGCTTATCATGT42590.1479531982937616No Hit
GATAAGCAGTGGTATCAACGCAGAG41660.14472247572007768No Hit
CTATCGGTCAGTCAGGAGTATTTAG40950.14225601009930824No Hit
ACGCAGAGTACATGGGAAGCAGTGG39680.1378441631438474No Hit
GTATTTAGCCTTGGAGGATGGTCCC37830.13141745694888476No Hit
CAGTGGTATCAACGCAGAGTACATG37460.13013211570989222No Hit
GTACACAAGCAGTGGTATCAACGCA37380.1298542040906506No Hit
GGATAAGCAGTGGTATCAACGCAGA37270.12947207561419335No Hit
TATCAACGCAGAGTACATGGGAAGC37100.1288815134233049No Hit
ACGCAGAGTACATGATAAGCAGTGG37010.12856886285165806No Hit
GCCCAGAGCCTGAATCAGTGTGTGT36760.12770038904152797No Hit
GGTATCAACGCAGAGTACATGGGGG36630.12724878266026032No Hit
GCCTTGGAGGATGGTCCCCCCATAT36610.12717930475544995No Hit
TATTCAGACAGGATACCACGTGTCC36560.12700560999342392No Hit
GGTATCAACGCAGAGTACATGGGAA36530.1269013931362083No Hit
GTATCAACGCAGAGTACATGGGGGT35670.12391384322936082No Hit
ACTCTGCGTTGATACCACTGCTTCC35070.1218295060850486No Hit
GTCAGGAGTATTTAGCCTTGGAGGA34340.11929356255946874No Hit
GTTCACTATCGGTCAGTCAGGAGTA33810.11745239808199297No Hit
GGTTAATGAGGCGAACCGGGGGAAC33790.11738292017718255No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG33330.1157849283665432No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA33130.11509014931843912No Hit
GGGATAAGCAGTGGTATCAACGCAG32210.11189416569716042No Hit
CACACACACTGATTCAGGCTCTGGG31950.11099095293462512No Hit
AGCGTACACGGTGGATGCCCTGGCA31820.11053934655335747No Hit
TCTAAGTACCCCGAGGAAAAGAAAT31770.11036565179133144No Hit
TACATAAGCAGTGGTATCAACGCAG31100.10803814198018281No Hit
ATGTACTCTGCGTTGATACCACTGC30310.10529376474017174No Hit
GAGTACATGGGATAAGCAGTGGTAT30010.10425159616801564No Hit
TATCAACGCAGAGTACATGATAAGC29870.10376525083434279No Hit
AAAAAGTACTCTGCGTTGATACCAC29810.10355681711991156No Hit
GTACATGGGGGTTAAGCGACTAAGC29760.10338312235788555No Hit
ACTTAGATGTTTCAGTTCCCCCGGT29220.10150721892800457No Hit
GTATCAACGCAGAGTACATGATAAG29140.10122930730876294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG654.8567017E-1019.0111625
AGGATCG250.006012867719.0111625
TAAGACC250.006012867719.0111625
TCGGTAC350.00216205416.2955653
CTCGTGT807.75799E-915.4323498
CCTATAC953.0195224E-1015.0090732
TCTAGAG1400.014.93763
GGTCTGG400.005219497314.2751
TTAAGCT400.005259407714.2586194
GTCTTAG759.4914867E-713.9577771
TAACTCG903.6150595E-813.7302835
CCGTATA500.001480404313.3233331
GCAGGAC500.001493268813.3080443
CGCGGTA500.001503865613.29556216
CGTTCCA500.001503865613.2955629
GTACAAA5200.013.1769221
CTCTAGT655.3666485E-513.1769221
ATCGGAC655.472746E-513.14945816
CTAGGAC957.337076E-813.0078633
TAGGACA1650.012.6743284
Produced by FastQC (version 0.11.5)