FastQCFastQC Report
Wed 25 May 2016
SRR1295281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350755
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38790.11576495446548613No Hit
GTGGTATCAACGCAGAGTACATGGG36570.10913958197480866No Hit
GGTATCAACGCAGAGTACTTTTTTT34030.10155920083682632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA600.00588394411.0818437
ATACCGT803.7778125E-410.6860656
TATACCG750.002641072710.1369665
ACTCGCG750.00265090610.1324258
AAGCCCG856.57548E-410.0624295
TCTATAC4250.010.0621293
GTCTCGC2550.09.694381
AGGACCG900.00111057419.5034065
CTAGGAC3200.09.5031213
ACACCGT1701.6705599E-89.4987236
TCGCGTT900.00111559099.49872310
ATAGTAC4150.09.1596353
CTACACT4800.09.10742954
GTCCGTA850.0073876388.9486591
TAGGACT4400.08.8554474
GGTATCA28450.08.7560031
CTATACT5800.08.6841464
GTAGGAC2304.0199666E-108.6767643
TAGGACC2201.5952537E-98.639464
TTCCGTA1107.1286707E-48.6386862
Produced by FastQC (version 0.11.5)