##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295281_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3350755
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05860619472328	31.0	30.0	33.0	26.0	34.0
2	29.888215044072158	31.0	30.0	34.0	25.0	34.0
3	30.24999500112661	31.0	30.0	34.0	26.0	34.0
4	33.62752573673694	35.0	35.0	37.0	28.0	37.0
5	33.815645727604675	35.0	35.0	37.0	28.0	37.0
6	34.08846095879884	36.0	35.0	37.0	29.0	37.0
7	33.94687018298861	36.0	35.0	37.0	28.0	37.0
8	33.941670757784436	36.0	35.0	37.0	28.0	37.0
9	35.03319251929789	38.0	35.0	39.0	27.0	39.0
10	34.92344650683204	38.0	34.0	39.0	27.0	39.0
11	35.346852873456875	39.0	35.0	39.0	28.0	39.0
12	35.35392142964795	39.0	35.0	39.0	27.0	39.0
13	35.256071542085294	38.0	35.0	39.0	27.0	39.0
14	35.45059814877543	39.0	34.0	41.0	25.0	41.0
15	36.0851085800066	39.0	35.0	41.0	27.0	41.0
16	36.151724909759146	39.0	36.0	41.0	27.0	41.0
17	35.94887779022937	39.0	35.0	41.0	27.0	41.0
18	35.92271294081483	39.0	35.0	41.0	27.0	41.0
19	36.00087681731431	39.0	35.0	41.0	27.0	41.0
20	36.00512272607218	39.0	35.0	41.0	27.0	41.0
21	35.98993450729761	39.0	35.0	41.0	27.0	41.0
22	35.715327142688736	39.0	34.0	41.0	26.0	41.0
23	35.39674789711572	39.0	34.0	40.0	25.0	41.0
24	35.74421108078627	39.0	34.0	41.0	26.0	41.0
25	34.39849078789705	38.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.38793846767520535
1101	2	0.6066213865377748
1101	3	0.3393592311647815
1101	4	0.2934427707037983
1101	5	0.27566051290101257
1101	6	0.3189535100607799
1101	7	0.2656010918632603
1101	8	0.35986460530482844
1101	9	0.2525962464208007
1101	10	0.5925813232071988
1101	11	0.4323509212764378
1101	12	0.6165793681594849
1101	13	0.5197206590522114
1101	14	1.0796332210904112
1101	15	0.8343334035957852
1101	16	0.6321356959106126
1101	17	0.9000514242485238
1101	18	0.9622073854547679
1101	19	0.8674274261303339
1101	20	0.9010410772845958
1101	21	0.9108457546121329
1101	22	0.7384888982856239
1101	23	-1.4451780466390218
1101	24	0.25997356987482334
1101	25	0.5633757252086156
1102	1	0.24924781552343234
1102	2	0.26167801945441127
1102	3	0.2593145707855413
1102	4	0.3762971807215081
1102	5	0.15391316132578226
1102	6	0.14298277227754852
1102	7	0.15056988209628486
1102	8	0.17012198016530533
1102	9	0.5146329995576835
1102	10	0.8819828848607258
1102	11	0.4806177246573924
1102	12	0.41013302411026586
1102	13	0.4735421764708505
1102	14	1.0594690526626707
1102	15	0.6593625592445278
1102	16	0.4365158248238785
1102	17	0.8955033001467925
1102	18	0.7146756984882501
1102	19	0.7888807075743856
1102	20	0.876780487840378
1102	21	0.9034081185055243
1102	22	0.8026198373907718
1102	23	0.6935874584187687
1102	24	0.8022233386065096
1102	25	0.5753138963809477
1103	1	-0.45708642443166525
1103	2	-1.653603122307537
1103	3	-0.5948766034681796
1103	4	-0.2048356977447341
1103	5	-0.4641042581676871
1103	6	-0.2823076492462846
1103	7	-0.17592537579380263
1103	8	-0.1551987285599168
1103	9	0.46626604303892094
1103	10	0.518784654539445
1103	11	0.22554063547826786
1103	12	0.03508265327774751
1103	13	-0.048945255483360484
1103	14	-1.11874445688116
1103	15	-0.4354528170543688
1103	16	-0.32470974101589434
1103	17	0.20825597988852707
1103	18	0.39716801887944797
1103	19	0.33621463558240094
1103	20	0.32128995533854265
1103	21	0.4674808762215932
1103	22	-0.02214707367197377
1103	23	0.7241991103162064
1103	24	0.4894178217983267
1103	25	0.3318002933067703
1104	1	-0.20234981676105335
1104	2	-0.27036601643551705
1104	3	-0.5238786103472997
1104	4	-0.4209117001991558
1104	5	-0.37407581565224746
1104	6	-0.2729757317056496
1104	7	-0.39989495032417466
1104	8	-0.29988109701503873
1104	9	-2.398212583557715
1104	10	-0.8315544812922084
1104	11	-0.6672486379920812
1104	12	-0.2887573748129313
1104	13	-0.10407884101356757
1104	14	-1.9156215151285068
1104	15	-0.7590068367234721
1104	16	-0.4412307471012724
1104	17	-0.12572424123408865
1104	18	0.046356400935188447
1104	19	0.18396664246152028
1104	20	0.17606135970357428
1104	21	0.30653930370655047
1104	22	0.38318997928373477
1104	23	0.7863224686440518
1104	24	0.4068654262095208
1104	25	0.11538395877437324
1105	1	-0.26261806519214304
1105	2	-0.19065600171055763
1105	3	-1.5771734659609002
1105	4	-0.5925535915383051
1105	5	-0.48870187766068796
1105	6	-0.32988404546414074
1105	7	-0.26446133826840423
1105	8	-0.2542814201460786
1105	9	0.13589359099147913
1105	10	0.3750093005680384
1105	11	-0.18555250679970925
1105	12	-0.15663673240738518
1105	13	-0.31678875932510664
1105	14	0.4153453800087519
1105	15	0.23775820084854615
1105	16	-0.2942683541785982
1105	17	0.1946872103430053
1105	18	0.03918178162211916
1105	19	0.04585434419929868
1105	20	-0.3371369071921464
1105	21	0.015960940046809924
1105	22	-0.002258971270784116
1105	23	0.24924456952170715
1105	24	0.1660571311493939
1105	25	0.3670658616831375
1106	1	-1.491257393808393
1106	2	-0.9441659303436971
1106	3	-1.096109726574479
1106	4	-1.370908637007112
1106	5	-0.8435394184819955
1106	6	-0.653451671138285
1106	7	-0.6070408533854277
1106	8	-0.7238784255891417
1106	9	-0.2767196563069092
1106	10	-1.833659256969824
1106	11	-1.2516571920364612
1106	12	-0.8147877761878206
1106	13	-1.0015400996952977
1106	14	-0.2295518242851884
1106	15	-0.9319865003527426
1106	16	-1.426464388043577
1106	17	-0.613881860198795
1106	18	-0.5800842666472121
1106	19	-0.44899616555049704
1106	20	-0.2673314156773898
1106	21	-0.48229713473618574
1106	22	-0.48188572186479917
1106	23	-0.6526033263348907
1106	24	-0.843240495746798
1106	25	-1.4271959824768246
1107	1	0.10488270856702187
1107	2	0.18452748665824714
1107	3	0.0837064965179124
1107	4	0.13516647119315195
1107	5	0.06706474099878079
1107	6	0.0670372439748661
1107	7	0.06347892492797769
1107	8	-0.017724782561600705
1107	9	0.5820435586421482
1107	10	0.06921513521693612
1107	11	0.1344287095295087
1107	12	-0.024073182478211663
1107	13	0.17882215851047079
1107	14	-1.09454864166689
1107	15	-0.45652190891131994
1107	16	-0.12335887203044393
1107	17	0.10522219017045842
1107	18	0.40847251344396796
1107	19	0.3754699798745449
1107	20	0.4307923834791012
1107	21	0.45022371702447117
1107	22	-0.002478551574583321
1107	23	0.6476137188423721
1107	24	0.5504756118354095
1107	25	0.2413504769856587
1108	1	-0.17480685029789456
1108	2	0.1768725258409738
1108	3	-0.03356366233915864
1108	4	-0.4357056841973659
1108	5	-0.4388708450631924
1108	6	-0.33530498095992556
1108	7	-0.2936090578204755
1108	8	-0.3129590360186896
1108	9	0.3259676386273398
1108	10	0.18036165569867535
1108	11	-0.039283324786431706
1108	12	-0.05659298714601135
1108	13	-0.23083095274728294
1108	14	0.031131955641754416
1108	15	-0.08046672075235506
1108	16	-0.156045639354069
1108	17	0.21159037224197874
1108	18	0.136262592566311
1108	19	0.09983269721327304
1108	20	0.14792059139491442
1108	21	0.15978287093355448
1108	22	0.24741403910922344
1108	23	0.017378718818285677
1108	24	0.055621385062075035
1108	25	-0.7454832807784655
1109	1	-0.7532620575053848
1109	2	-0.6605300501629827
1109	3	-0.7197846321861583
1109	4	-0.5585627307606345
1109	5	-0.8464986043063831
1109	6	-1.2200673838346177
1109	7	-0.8512618980530604
1109	8	-1.1654433022227693
1109	9	-0.7487096921704932
1109	10	-1.0814296993577273
1109	11	-1.2551157149449779
1109	12	-1.1924301634670584
1109	13	-1.2394006087980216
1109	14	-0.6497863242000292
1109	15	-1.157828475283921
1109	16	-1.373580362659844
1109	17	-1.396853174489884
1109	18	-1.8233551064628841
1109	19	-1.9837309895085014
1109	20	-1.6935653444380563
1109	21	-1.4242547778338022
1109	22	-1.491850807044365
1109	23	-0.9745222525551469
1109	24	-1.8056709180432406
1109	25	-0.9837344911836254
1110	1	0.16725519018708468
1110	2	0.27788847840913533
1110	3	0.09909392020449204
1110	4	0.29465398686587463
1110	5	0.23422756634663955
1110	6	0.3040127080800161
1110	7	0.11914955921736237
1110	8	0.04187724646886437
1110	9	0.3453427685098589
1110	10	0.32189567654202733
1110	11	0.21522929523717238
1110	12	0.27356595520360116
1110	13	0.2898055110940305
1110	14	-2.320337563611062
1110	15	-0.6349293235277287
1110	16	-0.07021291640708682
1110	17	-0.13381975096434218
1110	18	-2.7257805660168373
1110	19	-0.4292788169447732
1110	20	-1.8287599473469243
1110	21	-0.2572405975898633
1110	22	-1.7862441835371499
1110	23	-0.49572706757490437
1110	24	0.06565653602440591
1110	25	0.07459299335494052
1111	1	0.013187024568509287
1111	2	-1.168329426523428
1111	3	-0.24007025692946726
1111	4	0.17535243842146286
1111	5	0.10651699496392553
1111	6	0.20943104153890602
1111	7	0.10012589276242778
1111	8	0.09558186514518496
1111	9	-1.5032718906280351
1111	10	-0.18485661140508824
1111	11	-0.15860323633098972
1111	12	0.014777171970948189
1111	13	0.18859060569459984
1111	14	0.9696776066442467
1111	15	0.40877567560175976
1111	16	0.4977769630346103
1111	17	0.09036568241591425
1111	18	0.2723970380906451
1111	19	0.0792530344184712
1111	20	0.4718670949118433
1111	21	-0.2704869188470411
1111	22	0.01881211471395261
1111	23	0.2747780356627061
1111	24	-0.31524587370552126
1111	25	-1.5434433593236392
1112	1	0.31846720825373254
1112	2	0.46031427963657023
1112	3	0.16047192717487846
1112	4	0.46121351289620804
1112	5	0.2722011039455694
1112	6	0.21710489662124388
1112	7	0.2660213162450802
1112	8	0.10179692024133402
1112	9	-0.13475648120235917
1112	10	0.6612331104692615
1112	11	0.3352839351561627
1112	12	0.2656913170424815
1112	13	0.1967204840394885
1112	14	0.7281019566733278
1112	15	0.37120965076913137
1112	16	0.32679886010743786
1112	17	-3.5162362789476163
1112	18	-0.7443569901513385
1112	19	-0.9112885066182557
1112	20	0.08346359676998816
1112	21	-0.25822429090779053
1112	22	-1.4265678313136263
1112	23	-1.1344245393231063
1112	24	-0.22598692044879698
1112	25	0.26020813335601645
1113	1	-0.30071114745934224
1113	2	-0.14348637910107342
1113	3	-0.12660987031540216
1113	4	-0.32213387619249545
1113	5	-0.08435509586860235
1113	6	-0.1632425301246272
1113	7	-0.3556439448460509
1113	8	-0.19313442549434257
1113	9	0.2965905680961214
1113	10	0.3619120778923133
1113	11	-0.07110889386893149
1113	12	-0.061380199166301
1113	13	-0.36927369573931657
1113	14	-1.4116043699929364
1113	15	-1.484151590901405
1113	16	-0.42435778406125735
1113	17	-0.1461051094124386
1113	18	-0.5089154248743952
1113	19	-0.10688047285641744
1113	20	0.22997492325672653
1113	21	-1.7279069898930643
1113	22	-1.9275517341896702
1113	23	-0.6534593599392622
1113	24	-2.201866631206748
1113	25	-0.5567681978028389
1114	1	0.24511961348774847
1114	2	0.3210212932495473
1114	3	0.051390689340887974
1114	4	0.39465993720189374
1114	5	0.21198109168213364
1114	6	0.26245990751086623
1114	7	0.22846996954167054
1114	8	0.17725454898002369
1114	9	0.6971111434026085
1114	10	0.45976297993747295
1114	11	0.3845452073864237
1114	12	0.34538676793727063
1114	13	0.29536369757991565
1114	14	1.1877585090974137
1114	15	0.5264725682973648
1114	16	0.4244080862821633
1114	17	-0.2655205460444918
1114	18	0.015377137379580574
1114	19	-0.29624211453069194
1114	20	-1.5687436982676957
1114	21	-0.712520188315402
1114	22	-1.2298425415087024
1114	23	-2.5871079673046182
1114	24	-1.7078146855480085
1114	25	-0.9742357723827482
1115	1	0.5538728911430901
1115	2	0.5765562495127519
1115	3	0.5486375888321753
1115	4	0.6602369448803458
1115	5	0.7469701319117092
1115	6	0.5334707053552563
1115	7	0.6462351845516068
1115	8	0.6972201082391294
1115	9	0.9200675081789456
1115	10	1.1090809183168986
1115	11	0.7672874183881078
1115	12	0.711763486032595
1115	13	0.6465088194375426
1115	14	1.3212977574931202
1115	15	0.8555792478291977
1115	16	0.9031554448590029
1115	17	0.7475856297007084
1115	18	1.015028156257145
1115	19	0.4646353648392818
1115	20	1.005975917835741
1115	21	-0.034409404761042595
1115	22	1.043131675443476
1115	23	1.1686676740390851
1115	24	1.0833867404559925
1115	25	1.3184656545819564
1116	1	0.6389477094392859
1116	2	0.7847889659815301
1116	3	0.69774827706377
1116	4	0.8709064235894814
1116	5	0.8504078664386725
1116	6	0.7037444091900937
1116	7	0.7751924566834774
1116	8	0.7015935063529994
1116	9	0.8889047841059039
1116	10	0.8342251311864644
1116	11	0.7469450269220914
1116	12	0.7916355287535666
1116	13	0.3184734726245395
1116	14	1.3144298845442108
1116	15	0.915233411410874
1116	16	0.9455301015228486
1116	17	1.1386440200845769
1116	18	1.1504617013541605
1116	19	1.3404108963756158
1116	20	1.0618964919529148
1116	21	0.5816631967929098
1116	22	0.7425525391992451
1116	23	-0.10626084728041718
1116	24	0.40157226092976117
1116	25	-0.06577493483425201
1201	1	0.32615667088698785
1201	2	0.6118591812286027
1201	3	0.41508924394444335
1201	4	0.3697494220299973
1201	5	0.3430669672835691
1201	6	0.29410034300755683
1201	7	0.25403215453896877
1201	8	0.3163613299613317
1201	9	0.5297741814215655
1201	10	0.8794246942597752
1201	11	0.5121436473660097
1201	12	0.5917517264851497
1201	13	0.48348800390621705
1201	14	1.0724630703509774
1201	15	0.7754907464704317
1201	16	0.5321130298964221
1201	17	0.5240003139532945
1201	18	0.5324901126463075
1201	19	0.8643216860405829
1201	20	0.9194885493189631
1201	21	0.8167478489205067
1201	22	0.8306354507796385
1201	23	0.34056766783918135
1201	24	0.7445995884745358
1201	25	0.7508979538314051
1202	1	0.28801863306548015
1202	2	0.5332811785200064
1202	3	0.40038416115502784
1202	4	0.27554869868935583
1202	5	0.17164488840405312
1202	6	0.16080380265038485
1202	7	0.13461492734563762
1202	8	0.11618717523541022
1202	9	0.7106759833375023
1202	10	0.03271110594204174
1202	11	0.32369282355776363
1202	12	0.3118278658191116
1202	13	0.2564232224863261
1202	14	0.8487501244906923
1202	15	0.5285030286146579
1202	16	0.3388079743509067
1202	17	-1.4388638439898074
1202	18	-0.05893429769517411
1202	19	0.4277688465424845
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2210	20	0.8961418422840595
2210	21	0.9445724417396875
2210	22	1.2427514200951748
2210	23	1.5251104261820103
2210	24	1.2385087828528754
2210	25	1.2390133136537287
2211	1	0.17280152542796046
2211	2	-0.44066576045101513
2211	3	-0.024731315480828187
2211	4	0.3536939452969037
2211	5	0.29275516539106405
2211	6	0.24921362764123955
2211	7	0.2797793612850725
2211	8	0.3337160041334357
2211	9	0.7583764229456733
2211	10	0.8070222102810547
2211	11	0.42656881738358976
2211	12	0.30210588103320646
2211	13	0.23164137484363323
2211	14	-0.7456486702412732
2211	15	0.12136364895523144
2211	16	0.2561921116229584
2211	17	0.37923805294053636
2211	18	0.26599802263160655
2211	19	0.5666781756011403
2211	20	0.700506150073025
2211	21	0.6628766497699203
2211	22	-0.019908643934748227
2211	23	0.5506913284752386
2211	24	-0.12150734257421192
2211	25	0.2985694091687279
2212	1	0.0729752581072809
2212	2	-0.9922974903951065
2212	3	-0.25323488609225464
2212	4	0.20698607727265284
2212	5	0.16418520232763
2212	6	0.21270476695762142
2212	7	0.1830065992495662
2212	8	0.11733481924716926
2212	9	-2.1686125554462308
2212	10	-0.030190715845236582
2212	11	0.0500285211418543
2212	12	0.14268309482093144
2212	13	0.15315412859160915
2212	14	0.5408126437644754
2212	15	0.28270357485157405
2212	16	0.3188087810847904
2212	17	0.48281921108539905
2212	18	0.11622519489523597
2212	19	-0.6468507849362766
2212	20	0.2751954973695163
2212	21	0.39086801298793006
2212	22	-1.7056386468106055
2212	23	-0.30632745447964993
2212	24	0.3460318920798855
2212	25	-0.2899679080575197
2213	1	0.35874211794436306
2213	2	0.6690966563676
2213	3	0.31957598681890076
2213	4	0.5347826149661543
2213	5	0.6516571970688219
2213	6	0.5300024287383778
2213	7	0.4171968292462793
2213	8	0.44505568548573393
2213	9	0.9161139152184461
2213	10	0.9302183235479973
2213	11	0.3149330336733769
2213	12	0.2560652111146098
2213	13	0.25938084113212057
2213	14	-1.4542365477068486
2213	15	-0.10636049273753656
2213	16	0.2266520053377974
2213	17	-1.8177946897465134
2213	18	-0.5943604949864891
2213	19	-0.16033882690793888
2213	20	0.5128305016348307
2213	21	0.10019255981714537
2213	22	0.6398411755467066
2213	23	1.1538102733118905
2213	24	0.973412294206895
2213	25	-0.42041321949293575
2214	1	0.4025018527821125
2214	2	0.38582938879853756
2214	3	0.277774055403583
2214	4	0.675212364938858
2214	5	0.5485148234000334
2214	6	0.4901976037410307
2214	7	0.46270227825765176
2214	8	0.34941496805252825
2214	9	0.7753454248487088
2214	10	0.7925444849294792
2214	11	0.18303820466932308
2214	12	0.16334547761682927
2214	13	0.23133995367489035
2214	14	1.1903009162758167
2214	15	0.7527038873322738
2214	16	0.771826501087709
2214	17	0.6431125673789708
2214	18	0.5789462474828042
2214	19	-0.4180710484734007
2214	20	0.40737395289625056
2214	21	0.7858050334565903
2214	22	1.0130573521601178
2214	23	1.4442528720582786
2214	24	1.140697450442552
2214	25	1.2814136131249327
2215	1	0.1803324912907911
2215	2	0.46890391992632274
2215	3	0.369491590574313
2215	4	0.5584237244447579
2215	5	0.5919003140449632
2215	6	0.4834747479774748
2215	7	0.4724033865146495
2215	8	0.45295786458034826
2215	9	0.563755113483893
2215	10	0.8107077070336004
2215	11	0.48533947119949516
2215	12	0.3631420700010395
2215	13	0.26312368653600515
2215	14	1.1837055877172347
2215	15	0.7657005146226936
2215	16	0.6291746615432103
2215	17	0.8146739317154967
2215	18	0.7119215341738467
2215	19	0.029548636517162663
2215	20	-1.2966213453377406
2215	21	-1.5057610780025712
2215	22	0.2381430221705827
2215	23	0.7993783662597167
2215	24	0.8923279713764316
2215	25	0.36417662390701366
2216	1	0.30458331513595027
2216	2	0.18760932950948117
2216	3	0.1878156443382828
2216	4	0.5417043446423264
2216	5	0.6590144803715745
2216	6	0.42444281795278016
2216	7	0.5633286877910848
2216	8	0.5159086647646092
2216	9	0.8007025066507296
2216	10	-1.1094260437374714
2216	11	0.09005233415715708
2216	12	0.1803976873695916
2216	13	0.3923172003246691
2216	14	-0.022333504969104467
2216	15	0.054837531817426566
2216	16	0.45167956994199443
2216	17	0.7209630069705
2216	18	0.6338104209036572
2216	19	-0.9757647027828327
2216	20	-0.50470803949068
2216	21	-0.33413331846896455
2216	22	-0.196255851860073
2216	23	-3.861662073307251
2216	24	-1.4884095781695308
2216	25	-0.6449079049495694
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	19.0
4	33.0
5	64.0
6	79.0
7	284.0
8	236.0
9	666.0
10	1754.0
11	3567.0
12	6224.0
13	9922.0
14	13389.0
15	16822.0
16	19656.0
17	21101.0
18	22559.0
19	23172.0
20	23921.0
21	24326.0
22	25370.0
23	27216.0
24	29201.0
25	32225.0
26	36008.0
27	41400.0
28	47605.0
29	58189.0
30	71285.0
31	88027.0
32	115541.0
33	153049.0
34	205698.0
35	283333.0
36	400500.0
37	600142.0
38	948169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.160643920919036	21.12825941059934	13.244236380313328	23.46686028816829
2	15.643418298981612	21.257401646936405	40.46632651751486	22.63285353656713
3	18.267674646256303	26.14408997182111	30.14952811783605	25.438707264086535
4	12.617592413178889	17.18977233744563	38.14751773981329	32.045117509562196
5	13.336765159479889	37.65777268312171	35.23836510907743	13.767097048320966
6	31.004222517666456	38.369450296615	17.69179979069494	12.9345273950236
7	25.981394504680328	32.74917029305865	23.6189312288021	17.65050397345893
8	23.823785542432685	37.48144194137669	21.001856029768128	17.6929164864225
9	24.440434021324904	16.97841984353379	22.19258688711057	36.38855924803074
10	14.947199995223745	29.884101190929297	33.96373927021811	21.20495954362885
11	32.31256218374538	25.45673056113358	23.789959912846488	18.44074734227456
12	20.84383533184723	27.24141801157916	31.799832604810046	20.11491405176356
13	26.34438454144352	24.04697509746676	27.174747601392298	22.43389275969742
14	21.02885281668969	23.115064052518616	28.169345013027254	27.686738117764442
15	22.734353635483203	30.973735340023094	24.226354461851518	22.06555656264218
16	22.291387929166195	31.041402108583892	25.69707509956936	20.97013486268055
17	20.4298263446402	30.29408841011	28.460726270041203	20.815358975208596
18	20.537724767191413	29.926101279010588	29.221234948975404	20.314939004822598
19	21.154025825243355	28.775335188185064	29.40984731053961	20.660791676031966
20	22.203029212179175	28.51481324275904	28.78145579130422	20.50070175375756
21	22.597203973583465	28.83943234180793	27.850394260140654	20.712969424467946
22	21.657227254519068	28.421366522811898	29.20578197077255	20.71562425189649
23	22.002182618808252	28.650543386115775	28.56179987248157	20.7854741225944
24	21.476942382597084	27.984857995953018	28.815208627909605	21.722990993540293
25	22.208828034463195	28.276843221978336	28.27887475677599	21.235453986782478
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2146.0
1	2146.0
2	2484.5
3	2823.0
4	2823.0
5	2823.0
6	4316.0
7	5809.0
8	5809.0
9	5809.0
10	9421.0
11	13033.0
12	13033.0
13	13033.0
14	21765.0
15	30497.0
16	30497.0
17	30497.0
18	48210.0
19	65923.0
20	65923.0
21	65923.0
22	94081.5
23	122240.0
24	122240.0
25	122240.0
26	159663.0
27	197086.0
28	197086.0
29	197086.0
30	237742.5
31	278399.0
32	278399.0
33	278399.0
34	314298.5
35	350198.0
36	350198.0
37	350198.0
38	377636.5
39	405075.0
40	405075.0
41	405075.0
42	408118.0
43	411161.0
44	411161.0
45	411161.0
46	394381.5
47	377602.0
48	377602.0
49	377602.0
50	357314.5
51	337027.0
52	337027.0
53	337027.0
54	307407.5
55	277788.0
56	277788.0
57	277788.0
58	240469.5
59	203151.0
60	203151.0
61	203151.0
62	168525.0
63	133899.0
64	133899.0
65	133899.0
66	104099.5
67	74300.0
68	74300.0
69	74300.0
70	55121.0
71	35942.0
72	35942.0
73	35942.0
74	25930.5
75	15919.0
76	15919.0
77	15919.0
78	11243.5
79	6568.0
80	6568.0
81	6568.0
82	4671.0
83	2774.0
84	2774.0
85	2774.0
86	1888.0
87	1002.0
88	1002.0
89	1002.0
90	646.5
91	291.0
92	291.0
93	291.0
94	186.5
95	82.0
96	82.0
97	82.0
98	51.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07884790144310759
2	0.01957767726974965
3	0.03235091792745217
4	0.040826619672282814
5	0.05109296263081007
6	0.018533136561759964
7	0.02318880371737116
8	0.03378343089840946
9	0.034320623262518446
10	0.025367417194035374
11	0.023994592263534637
12	0.01787656811673787
13	0.026710398104307836
14	0.03694689704260681
15	0.03458921944457294
16	0.02474069276924156
17	0.02518835307266571
18	0.026859618205449222
19	0.01856298058198824
20	0.017219999671715776
21	0.012803084677930794
22	0.018413760480846853
23	0.01942845716860827
24	0.018025788217879254
25	0.10540907944627405
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3350755.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.40487984002257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.00959226382469	69.8839109847838
2	8.765857361681185	13.921037009981433
3	1.6438129172528773	3.91580301521825
4	0.5753679344614006	1.8274808679883798
5	0.2763793202827177	1.0972933358658157
6	0.17933232278367517	0.8543916925241921
7	0.11427493189943796	0.6351791065341145
8	0.0716063446344486	0.4548714553185308
9	0.05506548614531989	0.3935221479631399
>10	0.2829386938134935	3.9193869863039716
>50	0.01678214613575998	0.9141779016088872
>100	0.008010134420857254	1.0702429939234914
>500	5.277691310960421E-4	0.2715901728917542
>1k	4.5237353305249684E-4	0.8411123290941408
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3879	0.11576495446548613	No Hit
GTGGTATCAACGCAGAGTACATGGG	3657	0.10913958197480866	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3403	0.10155920083682632	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.984402022827691E-5	0.0	2.984402022827691E-5	0.0
4	0.0	2.984402022827691E-5	0.0	2.984402022827691E-5	0.0
5	0.0	2.984402022827691E-5	0.0	2.984402022827691E-5	0.0
6	0.0	2.984402022827691E-5	0.0	2.984402022827691E-5	0.0
7	0.0	2.984402022827691E-5	0.0	5.968804045655382E-5	2.984402022827691E-5
8	0.0	5.968804045655382E-5	0.0	5.968804045655382E-5	2.984402022827691E-5
9	0.0	5.968804045655382E-5	0.0	8.953206068483073E-5	2.984402022827691E-5
10	0.0	5.968804045655382E-5	0.0	8.953206068483073E-5	2.984402022827691E-5
11	2.984402022827691E-5	5.968804045655382E-5	0.0	8.953206068483073E-5	2.984402022827691E-5
12	2.984402022827691E-5	8.953206068483073E-5	2.984402022827691E-5	8.953206068483073E-5	2.984402022827691E-5
13	2.984402022827691E-5	1.1937608091310764E-4	2.984402022827691E-5	8.953206068483073E-5	2.984402022827691E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	60	0.005883944	11.081843	7
ATACCGT	80	3.7778125E-4	10.686065	6
TATACCG	75	0.0026410727	10.136966	5
ACTCGCG	75	0.002650906	10.132425	8
AAGCCCG	85	6.57548E-4	10.062429	5
TCTATAC	425	0.0	10.062129	3
GTCTCGC	255	0.0	9.69438	1
AGGACCG	90	0.0011105741	9.503406	5
CTAGGAC	320	0.0	9.503121	3
ACACCGT	170	1.6705599E-8	9.498723	6
TCGCGTT	90	0.0011155909	9.498723	10
ATAGTAC	415	0.0	9.159635	3
CTACACT	480	0.0	9.1074295	4
GTCCGTA	85	0.007387638	8.948659	1
TAGGACT	440	0.0	8.855447	4
GGTATCA	2845	0.0	8.756003	1
CTATACT	580	0.0	8.684146	4
GTAGGAC	230	4.0199666E-10	8.676764	3
TAGGACC	220	1.5952537E-9	8.63946	4
TTCCGTA	110	7.1286707E-4	8.638686	2
>>END_MODULE