FastQCFastQC Report
Wed 25 May 2016
SRR1295281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350755
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93410.27877299295233465No Hit
GGTATCAACGCAGAGTACTTTTTTT78710.23490228321676757No Hit
TATCAACGCAGAGTACTTTTTTTTT57380.17124498806985292No Hit
GTGGTATCAACGCAGAGTACATGGG42370.1264491137072093No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38150.11385493717087643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG551.9671062E-413.8132989
TCCGTAC1001.4311263E-712.3587293
TATACCG752.0616059E-411.4080585
TCGGAAC600.005893548511.07941613
ATCGCTA600.005893548511.07941610
GGACCGT951.3667286E-510.9961126
AGCGTCA2700.010.9151163
CCGATCT750.00265671610.1297529
CGACCGC750.00265671610.12975210
CCGGTCG750.00265671610.1297529
GGTATCA35200.09.8146481
GTCTTAG3600.09.7816021
CTTAGAC2800.09.1671893
TAGACAG5500.09.16101555
TACATCG1151.1027881E-49.093385
CAGCGTC3150.09.0540142
TACACCG950.0018129119.0063615
CTAGACA4200.08.8276634
TAGGACC1856.506525E-88.73594
GTACTAG2409.822543E-118.7241321
Produced by FastQC (version 0.11.5)