FastQCFastQC Report
Wed 25 May 2016
SRR1295280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4340318
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG64170.14784630987867708No Hit
GTATCAACGCAGAGTACTTTTTTTT62750.144574660197709No Hit
GGTATCAACGCAGAGTACTTTTTTT51030.11757203043647954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAC550.003065983812.0930073
GGTATCA41000.010.2024181
CTAGGAC4750.010.201773
ATATCGT1106.852597E-59.4986926
GTCTTAG4500.08.8730131
GCACTAG2002.5112968E-88.556121
CGTTATA1000.0029020118.5514842
GTCTAGG4250.08.5001961
GTCCTAC2502.3283064E-108.3659831
GTACTAG3750.08.3659831
AGTACCG1252.739764E-48.3615475
ATAGGAC2752.1827873E-118.2923483
GTGTAGA6600.08.2104181
GTCCTGG7650.08.2019441
AGTGCGG1050.00452269758.1413610
TAGGACT5150.08.1180074
GTCTTAC4000.08.080781
CTAAGAC4000.08.0764023
TTTATAC8000.08.0764023
TCCGTCA1304.1967217E-48.03698410
Produced by FastQC (version 0.11.5)