##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295280_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4340318
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.377423267143097	31.0	30.0	33.0	27.0	34.0
2	30.22605624749154	31.0	30.0	34.0	26.0	34.0
3	30.741383235053284	31.0	31.0	34.0	27.0	34.0
4	34.04341916882588	35.0	35.0	37.0	29.0	37.0
5	34.50629608245294	37.0	35.0	37.0	31.0	37.0
6	34.71233928020942	37.0	35.0	37.0	32.0	37.0
7	34.576511214155275	37.0	35.0	37.0	31.0	37.0
8	34.56540903224142	37.0	35.0	37.0	31.0	37.0
9	35.66805450660527	39.0	35.0	39.0	29.0	39.0
10	35.58839882239043	39.0	35.0	39.0	29.0	39.0
11	35.99575929689944	39.0	35.0	39.0	30.0	39.0
12	36.00235282299592	39.0	35.0	39.0	30.0	39.0
13	35.93381613973907	39.0	35.0	39.0	30.0	39.0
14	36.23657091485002	39.0	36.0	41.0	27.0	41.0
15	36.84583088151606	40.0	36.0	41.0	30.0	41.0
16	36.952617296704986	40.0	36.0	41.0	30.0	41.0
17	36.74837696224102	40.0	36.0	41.0	29.0	41.0
18	36.70164375052703	40.0	36.0	41.0	29.0	41.0
19	36.755226229967484	40.0	36.0	41.0	29.0	41.0
20	36.74487583628665	40.0	36.0	41.0	29.0	41.0
21	36.744881365835404	40.0	36.0	41.0	29.0	41.0
22	36.51201870462026	39.0	36.0	41.0	27.0	41.0
23	36.19317317302557	39.0	35.0	41.0	27.0	41.0
24	36.5128280923195	39.0	36.0	41.0	27.0	41.0
25	35.40189105959517	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3703027405802821
1101	2	0.5802130956843357
1101	3	0.4221983656227728
1101	4	0.4243861657979693
1101	5	0.42661922906774663
1101	6	0.3253697046346744
1101	7	0.3460183527708054
1101	8	0.4323080293441137
1101	9	0.4654033625230838
1101	10	0.7203574267021509
1101	11	0.55049754079932
1101	12	0.6885476461747118
1101	13	0.605643113939152
1101	14	1.156000820527602
1101	15	0.8865554056353915
1101	16	0.7807731505230748
1101	17	1.0149260713575359
1101	18	0.9368439545362435
1101	19	1.0475451082362852
1101	20	1.1210916599170488
1101	21	1.1713426351911878
1101	22	0.9255626729535393
1101	23	-1.3447574393329376
1101	24	0.3549737521378731
1101	25	0.8071774045608962
1102	1	0.1781187474861916
1102	2	0.22761915244666397
1102	3	0.20429374465489047
1102	4	0.3693504911507475
1102	5	0.12336746295249412
1102	6	0.17620933608473877
1102	7	0.08942197837199473
1102	8	0.14659990961823155
1102	9	0.4114388081478708
1102	10	0.7989787116941329
1102	11	0.5260407643780596
1102	12	0.45442154290321213
1102	13	0.2937297196506208
1102	14	0.9975870650883181
1102	15	0.6818484662558646
1102	16	0.504460059248828
1102	17	0.8227778409962099
1102	18	0.8112461179173209
1102	19	0.7672350471049185
1102	20	0.8390996933190848
1102	21	0.8629195426837413
1102	22	0.7635775770283786
1102	23	0.8854382867562407
1102	24	0.8845793050508632
1102	25	0.8020601626955965
1103	1	-0.3345984485905227
1103	2	-1.5775853555641284
1103	3	-0.3572460678827518
1103	4	-0.08662883121500187
1103	5	-0.058505409657470864
1103	6	0.03494817018877683
1103	7	0.08882488979563163
1103	8	0.2290317469138543
1103	9	0.8325042342626077
1103	10	0.8542550634739072
1103	11	0.6216235399140331
1103	12	0.4571729894621157
1103	13	0.2701301027466414
1103	14	-0.6655688077683735
1103	15	0.00563554552012846
1103	16	0.0834573770460807
1103	17	0.5270445066016691
1103	18	0.7048699303594503
1103	19	0.6547441535944643
1103	20	0.6633900238587884
1103	21	0.7295858379276723
1103	22	0.31879641134465686
1103	23	1.1012566504268548
1103	24	0.9228411527728468
1103	25	0.8228094550026341
1104	1	-9.262351054459828E-4
1104	2	0.1244533116110631
1104	3	-0.25090402831523306
1104	4	-0.15440064291303912
1104	5	-0.0014101054596622475
1104	6	0.08548524120333667
1104	7	0.10937731370592729
1104	8	0.12078241440650572
1104	9	-2.063620085626212
1104	10	-0.4407112720994206
1104	11	-0.3260673923514972
1104	12	0.199328142572881
1104	13	0.339492237000826
1104	14	-1.2901830359499797
1104	15	-0.15858654898129743
1104	16	0.02698175185356888
1104	17	0.37050754222371296
1104	18	0.5566541870234829
1104	19	0.5862832864951173
1104	20	0.6864672695630674
1104	21	0.7371213556349403
1104	22	0.8115511504342905
1104	23	1.1885128596891406
1104	24	0.8766311656909025
1104	25	0.6654691778241997
1105	1	-0.23042670519251018
1105	2	-0.02596059251730054
1105	3	-1.343470891169254
1105	4	-0.460388928549861
1105	5	-0.36146970002826606
1105	6	-0.11510732339492336
1105	7	-0.09573295294076445
1105	8	-0.013732951426142392
1105	9	0.41316872046640185
1105	10	0.5564929316986493
1105	11	-0.018887878920573087
1105	12	-0.05968090273222515
1105	13	-0.12586883473447585
1105	14	0.6725915826348796
1105	15	0.3102199933919465
1105	16	-0.1713848246296905
1105	17	0.31668028252478564
1105	18	0.23085699288395034
1105	19	0.2721970586451903
1105	20	-0.2569247630352507
1105	21	0.1698629090773025
1105	22	0.24871803165783746
1105	23	0.4999262639220987
1105	24	0.31494748509562953
1105	25	0.6132818510259526
1106	1	-1.3702734515482113
1106	2	-0.875413411319844
1106	3	-0.9243746919034557
1106	4	-1.1782984653972903
1106	5	-0.6761289020484611
1106	6	-0.5244109101318415
1106	7	-0.5249874042942722
1106	8	-0.5764170835822497
1106	9	-0.08727600405317304
1106	10	-1.6315755556491283
1106	11	-0.9711942839579066
1106	12	-0.5582880716412646
1106	13	-0.7145441878893379
1106	14	0.11176109696877035
1106	15	-0.6905371909004359
1106	16	-1.2197211418131104
1106	17	-0.44696854920889706
1106	18	-0.4017035521883088
1106	19	-0.4596938547781164
1106	20	-0.06947845635998107
1106	21	-0.12822999075937247
1106	22	-0.12402399571080736
1106	23	-0.47884518716026037
1106	24	-0.5423930599949003
1106	25	-1.2909544781172144
1107	1	0.018219954181400766
1107	2	0.12400901447685087
1107	3	-0.004882329044345823
1107	4	0.14026567648010513
1107	5	-0.043292283598262316
1107	6	0.08946698065028613
1107	7	0.03869147363603531
1107	8	-0.12652625274498774
1107	9	0.4628738671851096
1107	10	0.058104182045759956
1107	11	0.039819580173222846
1107	12	-0.04681542781604975
1107	13	0.06817200155884251
1107	14	-0.9345015123953573
1107	15	-0.38340563175027853
1107	16	-0.055934387358149706
1107	17	0.09235830896904673
1107	18	0.3668782272551496
1107	19	0.33935909018264
1107	20	0.47069305000781014
1107	21	0.4636219344488026
1107	22	-0.13441904941511496
1107	23	0.640522409415297
1107	24	0.49525790891747334
1107	25	0.11095640420764141
1108	1	-0.10398058687120937
1108	2	0.19183318214456335
1108	3	0.011073058530978841
1108	4	-0.19232385600746937
1108	5	-0.22565455445661797
1108	6	-0.15185518004429355
1108	7	-0.11617966013287173
1108	8	-0.1571840125527828
1108	9	0.2941269801908817
1108	10	0.2895573087243406
1108	11	0.06400287395307203
1108	12	-0.011907182135189487
1108	13	-0.12045358808106954
1108	14	0.12336279473501577
1108	15	-0.043364027343727685
1108	16	-0.0630770631270039
1108	17	0.27247062133415056
1108	18	0.2386757134114319
1108	19	0.24352792984941374
1108	20	0.277425346160868
1108	21	0.3174715140099096
1108	22	0.29768920017225753
1108	23	0.044659996945689784
1108	24	0.0773084397154804
1108	25	-0.6777094225578608
1109	1	-0.7246244475642314
1109	2	-0.6553706095300953
1109	3	-0.7307601333623985
1109	4	-0.5522759289969912
1109	5	-0.8554611881531429
1109	6	-1.0589218670869869
1109	7	-0.8457815581378654
1109	8	-1.0811034708973608
1109	9	-0.8009829907299277
1109	10	-1.042643724600211
1109	11	-1.0818008030522606
1109	12	-1.0755178353276662
1109	13	-1.05719986214077
1109	14	-0.5841381834225388
1109	15	-1.1508610820327547
1109	16	-1.3458165545717833
1109	17	-1.3816160613081863
1109	18	-1.9379070972443628
1109	19	-2.0611920027796486
1109	20	-1.7706388123039076
1109	21	-1.44010703393824
1109	22	-1.6494648870089463
1109	23	-1.1958860805070444
1109	24	-2.070295155568985
1109	25	-1.1225548194730592
1110	1	0.10706846709459228
1110	2	0.23312528304261448
1110	3	0.08012684036822648
1110	4	0.200148894344359
1110	5	0.1845460581430629
1110	6	0.23109290228566692
1110	7	0.15387022473640855
1110	8	0.12265256620981546
1110	9	0.4965761525067762
1110	10	0.4212807742357185
1110	11	0.3100993139455923
1110	12	0.2942561593845383
1110	13	0.3003241498378699
1110	14	-2.3256540716857543
1110	15	-0.5810771111607309
1110	16	-0.059828506476861776
1110	17	-0.08288987119844649
1110	18	-2.6584079073654436
1110	19	-0.39053702779290944
1110	20	-1.7093206089335524
1110	21	-0.11813358914783123
1110	22	-1.647845291520241
1110	23	-0.39373669661036104
1110	24	0.20755240646707307
1110	25	0.18686103713718438
1111	1	-0.17994560925520986
1111	2	-1.3380173122559498
1111	3	-0.37706688779523745
1111	4	0.058355493930072555
1111	5	-0.0392315117865607
1111	6	-0.0038116632358722313
1111	7	0.014828685802044106
1111	8	0.028620785413430383
1111	9	-1.601016877109366
1111	10	-0.26114481372122356
1111	11	-0.17520190130040447
1111	12	-0.11963811987240547
1111	13	0.017803587849002156
1111	14	0.8232945569047558
1111	15	0.22633058595199174
1111	16	0.2090811450372314
1111	17	-0.11559100589403926
1111	18	0.08565288711008634
1111	19	-0.08793572678181505
1111	20	0.23584603387865855
1111	21	-0.5566567651415966
1111	22	-0.2418532178576669
1111	23	-0.11457406578529117
1111	24	-0.4684563320314652
1111	25	-2.0033693969363
1112	1	0.18381899554293568
1112	2	0.3544371380864
1112	3	0.03106041873274279
1112	4	0.34374959657704096
1112	5	0.18777145104228055
1112	6	0.13691213446125516
1112	7	0.07570858199998298
1112	8	0.024533976222997467
1112	9	-0.40764416661485825
1112	10	0.4981217170607053
1112	11	0.1689647822869702
1112	12	0.09953343000905335
1112	13	0.05516687933551623
1112	14	0.5000093893233313
1112	15	0.147244681319755
1112	16	0.1857278951390171
1112	17	-3.546232460551856
1112	18	-0.9172245621243533
1112	19	-1.0076879419570446
1112	20	-0.09210222661395306
1112	21	-0.5384732370776177
1112	22	-1.549121446002971
1112	23	-1.369775046402708
1112	24	-0.3212750956675876
1112	25	-0.05650490097521299
1113	1	-0.237466689692166
1113	2	-0.1527398101246895
1113	3	-0.12398831743248095
1113	4	-0.5306345680378541
1113	5	-0.28296335470575684
1113	6	-0.27755753617771006
1113	7	-0.37701559585319444
1113	8	-0.2529141947631288
1113	9	0.14021417240996215
1113	10	0.2448273370117846
1113	11	-0.21565150598677718
1113	12	-0.11489232906552616
1113	13	-0.5471823083410499
1113	14	-1.4979534219565949
1113	15	-1.7680546100188934
1113	16	-0.5592046536194246
1113	17	-0.36611321877980174
1113	18	-0.7361707818004604
1113	19	-0.35661636205622926
1113	20	0.08882233056385047
1113	21	-1.649686160364439
1113	22	-2.044603091860253
1113	23	-0.8117587148655403
1113	24	-2.4423243714103364
1113	25	-0.7529755292427538
1114	1	0.06121033673243659
1114	2	0.16381634084645924
1114	3	-0.16260958489367638
1114	4	0.15383230231810785
1114	5	-0.16932735317269731
1114	6	-0.05299088640095562
1114	7	0.011964713816851713
1114	8	-0.10347453108326476
1114	9	0.20552932469524876
1114	10	0.10061414881369757
1114	11	-0.007987962205007193
1114	12	0.013780056431137666
1114	13	-0.07051795878227551
1114	14	0.6731837721327949
1114	15	0.1379503663756836
1114	16	0.08509702909407224
1114	17	-0.6451513692423134
1114	18	-0.3418127257843864
1114	19	-0.682546042865873
1114	20	-1.9221472093413325
1114	21	-1.0615632512681614
1114	22	-1.6721030221774456
1114	23	-3.1163717096262786
1114	24	-2.143647521621496
1114	25	-1.7188516731668386
1115	1	0.31103191753562953
1115	2	0.38089564542090315
1115	3	0.2487218444467203
1115	4	0.45521261058868845
1115	5	0.27039989204434534
1115	6	0.19382317943628635
1115	7	0.2807610201362394
1115	8	0.26167154961302685
1115	9	0.496854905597786
1115	10	0.7234045346189291
1115	11	0.3646554534230759
1115	12	0.2636843258852579
1115	13	0.19042118790459028
1115	14	0.8541491585049172
1115	15	0.37805187179841226
1115	16	0.39950640975624196
1115	17	0.38415014128244707
1115	18	0.5159902956430571
1115	19	-0.01028877868223077
1115	20	0.48364350674609824
1115	21	-0.5438159992106151
1115	22	0.5297902526724059
1115	23	0.6629847185803399
1115	24	0.4970077463727449
1115	25	0.5832161606138868
1116	1	0.5144856868471699
1116	2	0.6852613712010971
1116	3	0.3380312330299162
1116	4	0.6767018885120777
1116	5	0.3746656770411718
1116	6	0.28063557463709543
1116	7	0.28571672467170117
1116	8	0.2347278552685239
1116	9	0.3863715015165141
1116	10	0.3566293634808346
1116	11	0.2508430215934254
1116	12	0.2079527766479501
1116	13	-0.3137759943198404
1116	14	0.7062853832106697
1116	15	0.3802487121536302
1116	16	0.40377036331086913
1116	17	0.5670913764070633
1116	18	0.5309686947997818
1116	19	0.6229187532255622
1116	20	0.4806086544010384
1116	21	-0.06843376196152207
1116	22	0.0496968847315955
1116	23	-0.6691588980296217
1116	24	-0.30947698346918173
1116	25	-0.9435788562353835
1201	1	0.3090311931438592
1201	2	0.5438793890253706
1201	3	0.5423626962270269
1201	4	0.3792640489305512
1201	5	0.45928243616045705
1201	6	0.3134457125886314
1201	7	0.3394659577471799
1201	8	0.4436376206686816
1201	9	0.7055223184097272
1201	10	0.9139761841438343
1201	11	0.6035435446833901
1201	12	0.6105710727788534
1201	13	0.5445314586052277
1201	14	1.1310877222513938
1201	15	0.8165906561254417
1201	16	0.6072322764159495
1201	17	0.765576344686437
1201	18	0.7373627896390857
1201	19	0.9409085887083961
1201	20	0.9984612341137478
1201	21	0.9469393586780797
1201	22	1.0493646561912513
1201	23	0.655567697331513
1201	24	0.9666185326314931
1201	25	1.158736939563454
1202	1	0.3805307724054465
1202	2	0.742171661975064
1202	3	0.5685471618687714
1202	4	0.4831728725623279
1202	5	0.48047603315653475
1202	6	0.3884551305773982
1202	7	0.3884861286222545
1202	8	0.36359566967088597
1202	9	0.9947228540066533
1202	10	0.23998129852121508
1202	11	0.6168151287686285
1202	12	0.5470789337283932
1202	13	0.6043430578820903
1202	14	1.2238805551619407
1202	15	0.9098468131356015
1202	16	0.7397055016880998
1202	17	-1.1945926263163997
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2210	19	0.6330121761770613
2210	20	0.790181815397375
2210	21	0.8047926801890597
2210	22	1.0440024792157345
2210	23	1.310425616724416
2210	24	1.0441000737670336
2210	25	0.9672167642889775
2211	1	0.08276017166393501
2211	2	-0.5488763613131482
2211	3	-0.08952846953826565
2211	4	0.3125422453007687
2211	5	0.17444159575769191
2211	6	0.07153804824498167
2211	7	0.12478345357890674
2211	8	0.21922737660428027
2211	9	0.6380306929932331
2211	10	0.7156119016194395
2211	11	0.2732926606871402
2211	12	0.0761809787275638
2211	13	0.08273119387620653
2211	14	-1.0052149002484256
2211	15	-0.10018636650539747
2211	16	0.09201393762187138
2211	17	0.25076821057854204
2211	18	0.16829701996049806
2211	19	0.4650412834582909
2211	20	0.5429790030871118
2211	21	0.5339360446430561
2211	22	-0.11171337622239008
2211	23	0.4781657379071831
2211	24	-0.19130651272057975
2211	25	0.14892021831917646
2212	1	-0.12258155418952299
2212	2	-1.1301464849861382
2212	3	-0.44953365543524626
2212	4	0.01382251552632141
2212	5	-0.062010472671332195
2212	6	-0.02643266605868888
2212	7	-0.05665897594968783
2212	8	-0.031467144763183796
2212	9	-2.3613974610788304
2212	10	-0.15037163968583656
2212	11	-0.08862589366314211
2212	12	-0.22460175232711777
2212	13	-0.20254830503115073
2212	14	0.4391537086818573
2212	15	0.11641584517047932
2212	16	0.1835559377850018
2212	17	0.26339152141834177
2212	18	-0.036223471920152406
2212	19	-0.6036500336402355
2212	20	0.13083522355889698
2212	21	0.2052863708622823
2212	22	-1.919442199011371
2212	23	-0.3887083849994539
2212	24	0.14209909510699248
2212	25	-0.6436054969477851
2213	1	0.10479784676488535
2213	2	0.39526745957505227
2213	3	0.12140594897843826
2213	4	0.30315101203431993
2213	5	0.14454663189668793
2213	6	0.060624521019839506
2213	7	0.04154763349562529
2213	8	0.06754189433469548
2213	9	0.4368398134746059
2213	10	0.535484719003648
2213	11	-0.17147978694397636
2213	12	-0.10580160104714054
2213	13	-0.14626749372943948
2213	14	-1.9712816743948736
2213	15	-0.6399270393548875
2213	16	-0.37128284290866276
2213	17	-2.3435350321161224
2213	18	-1.0150133807054473
2213	19	-0.7291537078118608
2213	20	0.056429361175425186
2213	21	-0.38000490776265394
2213	22	0.08868995283622638
2213	23	0.6388074174379952
2213	24	0.38185806350450235
2213	25	-1.1222330540284133
2214	1	0.2514937519226166
2214	2	0.17437757487390115
2214	3	0.14792034150784517
2214	4	0.3588345294063231
2214	5	0.2595802778605574
2214	6	0.20980586961682235
2214	7	0.20530468131506296
2214	8	0.11490805448168828
2214	9	0.48415148004639263
2214	10	0.3812231056154971
2214	11	-0.08046226596292883
2214	12	-0.17607706979979554
2214	13	-0.06534236759981837
2214	14	0.807691514251033
2214	15	0.3711238775909891
2214	16	0.2923029533445174
2214	17	0.1893284924725478
2214	18	0.19471615585138835
2214	19	-0.8308565165844399
2214	20	0.01754247592995739
2214	21	0.3461583226974767
2214	22	0.5713775056279928
2214	23	0.9872155718295943
2214	24	0.6988902808770305
2214	25	0.6403871597665045
2215	1	-0.1682499717903312
2215	2	0.20216491401370007
2215	3	0.03616961241728234
2215	4	0.288381974508475
2215	5	0.1813823978468605
2215	6	0.073322548588024
2215	7	-0.0030715485896166683
2215	8	0.022371983617254898
2215	9	0.11131009787732182
2215	10	0.41879784186787106
2215	11	0.1507929126763088
2215	12	-0.06133514590312217
2215	13	-0.08856530080042546
2215	14	0.6877975795541502
2215	15	0.13948064757599354
2215	16	0.17044302296228864
2215	17	0.31823470616938465
2215	18	0.21017778514686114
2215	19	-0.5072210300461961
2215	20	-1.7623363830815322
2215	21	-2.036796769372998
2215	22	-0.3814192333585069
2215	23	0.2628333109597776
2215	24	0.394579680299465
2215	25	-0.21579003806240848
2216	1	0.027880403454741298
2216	2	-0.11173149690398532
2216	3	-0.11700595181721951
2216	4	0.1351767338735428
2216	5	0.050172458723004354
2216	6	-0.1346962717174236
2216	7	-0.08435619747515233
2216	8	-0.10628211257413511
2216	9	0.1439187802830162
2216	10	-1.7598201140012435
2216	11	-0.6405532546297508
2216	12	-0.3357471389896105
2216	13	-0.20227963497941914
2216	14	-0.6767603499487507
2216	15	-0.7244718800644776
2216	16	-0.3255145638292447
2216	17	0.10702573869238563
2216	18	-0.07857375569565761
2216	19	-1.4504889913087808
2216	20	-1.1300792886185178
2216	21	-1.1024374757767248
2216	22	-0.8108066594248271
2216	23	-4.712814188737614
2216	24	-2.244327496959386
2216	25	-1.3952941687966671
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	20.0
5	33.0
6	76.0
7	391.0
8	222.0
9	515.0
10	1263.0
11	2680.0
12	5049.0
13	8226.0
14	11883.0
15	15202.0
16	18033.0
17	19419.0
18	21132.0
19	21807.0
20	22543.0
21	23027.0
22	24188.0
23	25915.0
24	28570.0
25	31790.0
26	36428.0
27	42517.0
28	50787.0
29	63161.0
30	78209.0
31	98734.0
32	133140.0
33	182316.0
34	251902.0
35	358402.0
36	527885.0
37	827628.0
38	1407217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46017517504246	20.830634533948377	13.510153211869106	23.19903707914006
2	16.03484057519487	20.875378899781495	40.32364735600887	22.766133169014765
3	18.169737554125025	25.96563101229123	30.20077307636641	25.66385835721733
4	12.978490224662774	17.265901849045516	38.051478256861174	31.704129669430536
5	13.53669302882011	37.41923661166035	35.40779892538304	13.636271434136491
6	30.940319246749116	38.44453047407704	17.518406116706878	13.096744162466962
7	26.45472346271967	32.66781627922358	23.37722462819451	17.500235629862242
8	23.70604424921744	37.044976862634556	21.11952718966002	18.129451698487987
9	24.518895013158446	17.121768989397182	22.057742278833686	36.30159371861069
10	15.103405936340971	29.81218655702087	33.768763945202295	21.31564356143586
11	32.1524322655132	25.43256279137294	23.75409333612944	18.660911606984424
12	21.083972121822427	27.252404890318104	31.392307346653315	20.27131564120615
13	26.417147126482345	24.04028257443805	26.986209124811083	22.556361174268524
14	21.06910932833258	23.235987356132675	28.007194115263502	27.687709200271243
15	22.94266621000125	30.72516224913557	24.19022941905613	22.14194212180705
16	22.278603823675816	30.830829077635	25.65361073726517	21.236956361424014
17	20.40594718128339	30.085729765380993	28.323546498966547	21.18477655436907
18	20.506014167032337	29.724909934990944	29.1604825714528	20.608593326523913
19	21.32543763471932	28.643752917910454	29.29386221425217	20.73694723311806
20	22.47523301429056	28.28033517925878	28.579852559470257	20.6645792469804
21	22.84998323661262	28.651543902217032	27.6845211143793	20.81395174679105
22	21.870400477480416	28.23181305443438	29.009562282734315	20.888224185350893
23	22.120589929715408	28.511072704228596	28.303352920843416	21.06498444521258
24	21.758412386328914	27.794922633837032	28.60679148306231	21.839873496771744
25	22.377479308772415	28.070435630405765	28.09905956199618	21.453025498825635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2476.0
1	2476.0
2	2924.5
3	3373.0
4	3373.0
5	3373.0
6	5220.0
7	7067.0
8	7067.0
9	7067.0
10	11540.0
11	16013.0
12	16013.0
13	16013.0
14	26905.0
15	37797.0
16	37797.0
17	37797.0
18	60303.0
19	82809.0
20	82809.0
21	82809.0
22	119070.0
23	155331.0
24	155331.0
25	155331.0
26	202613.0
27	249895.0
28	249895.0
29	249895.0
30	302186.5
31	354478.0
32	354478.0
33	354478.0
34	400426.5
35	446375.0
36	446375.0
37	446375.0
38	481015.0
39	515655.0
40	515655.0
41	515655.0
42	521725.0
43	527795.0
44	527795.0
45	527795.0
46	508531.5
47	489268.0
48	489268.0
49	489268.0
50	468725.5
51	448183.0
52	448183.0
53	448183.0
54	409019.0
55	369855.0
56	369855.0
57	369855.0
58	320922.0
59	271989.0
60	271989.0
61	271989.0
62	226046.0
63	180103.0
64	180103.0
65	180103.0
66	140057.5
67	100012.0
68	100012.0
69	100012.0
70	74179.5
71	48347.0
72	48347.0
73	48347.0
74	34536.0
75	20725.0
76	20725.0
77	20725.0
78	14481.0
79	8237.0
80	8237.0
81	8237.0
82	5670.5
83	3104.0
84	3104.0
85	3104.0
86	2082.5
87	1061.0
88	1061.0
89	1061.0
90	670.0
91	279.0
92	279.0
93	279.0
94	175.0
95	71.0
96	71.0
97	71.0
98	45.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08093876992423137
2	0.01797103345883873
3	0.029721324566540978
4	0.03670237987170525
5	0.0467246869929807
6	0.016772964561582813
7	0.02039021103983625
8	0.038361244498674986
9	0.03824604556624653
10	0.02331626392351897
11	0.021841717588434763
12	0.016657765629154362
13	0.024698651112660407
14	0.040826501652643885
15	0.0373244541068189
16	0.023915298372146926
17	0.02269418968840532
18	0.028338937377399537
19	0.017049441999411104
20	0.015436656945412757
21	0.011589012602302412
22	0.020113733602007963
23	0.018846545345294976
24	0.018984784064209118
25	0.1102453783340299
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4340318.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.65033083145217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.85816505795984	61.76181599437264
2	11.476405238533822	16.904820851476533
3	2.4397484946778865	5.390648513356912
4	0.8179147644506847	2.4095877197488877
5	0.3874835661881373	1.42691464207536
6	0.23680442084317144	1.046443436247
7	0.15396553064914392	0.7937728588264676
8	0.10396682735668145	0.6125752984252847
9	0.07500474343575404	0.4971711751174327
>10	0.4174970367868404	5.356397173661276
>50	0.0221804667139614	1.130628151742328
>100	0.009606819218107342	1.1978593258489512
>500	6.913684815223923E-4	0.3605795507720559
>1k	4.713872537694127E-4	0.6989161517673883
>5k	9.42774507537458E-5	0.41186915656162015
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6417	0.14784630987867708	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6275	0.144574660197709	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5103	0.11757203043647954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.303978648569068E-5	0.0
3	0.0	0.0	0.0	2.303978648569068E-5	0.0
4	0.0	0.0	0.0	2.303978648569068E-5	0.0
5	0.0	0.0	0.0	2.303978648569068E-5	0.0
6	0.0	0.0	0.0	2.303978648569068E-5	0.0
7	0.0	0.0	0.0	2.303978648569068E-5	0.0
8	0.0	0.0	0.0	2.303978648569068E-5	0.0
9	0.0	0.0	0.0	4.607957297138136E-5	0.0
10	0.0	0.0	0.0	4.607957297138136E-5	0.0
11	0.0	0.0	0.0	9.215914594276271E-5	0.0
12	0.0	0.0	0.0	1.382387189141441E-4	0.0
13	0.0	0.0	0.0	1.382387189141441E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAC	55	0.0030659838	12.093007	3
GGTATCA	4100	0.0	10.202418	1
CTAGGAC	475	0.0	10.20177	3
ATATCGT	110	6.852597E-5	9.498692	6
GTCTTAG	450	0.0	8.873013	1
GCACTAG	200	2.5112968E-8	8.55612	1
CGTTATA	100	0.002902011	8.551484	2
GTCTAGG	425	0.0	8.500196	1
GTCCTAC	250	2.3283064E-10	8.365983	1
GTACTAG	375	0.0	8.365983	1
AGTACCG	125	2.739764E-4	8.361547	5
ATAGGAC	275	2.1827873E-11	8.292348	3
GTGTAGA	660	0.0	8.210418	1
GTCCTGG	765	0.0	8.201944	1
AGTGCGG	105	0.0045226975	8.14136	10
TAGGACT	515	0.0	8.118007	4
GTCTTAC	400	0.0	8.08078	1
CTAAGAC	400	0.0	8.076402	3
TTTATAC	800	0.0	8.076402	3
TCCGTCA	130	4.1967217E-4	8.036984	10
>>END_MODULE