FastQCFastQC Report
Wed 25 May 2016
SRR1295280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4340318
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT152690.351794499850011No Hit
GGTATCAACGCAGAGTACTTTTTTT127450.2936420787601277No Hit
TATCAACGCAGAGTACTTTTTTTTT92850.21392441751963798No Hit
GTGGTATCAACGCAGAGTACATGGG70920.1633981657565183No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59650.1374323263871449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC701.0957017E-412.2095828
GAACCGT550.003077507512.086956
AGCGTCA3800.011.0079383
ATACCGT1053.483925E-610.8535886
GGTATCA53100.010.3062671
TATACCG856.5544003E-410.0660835
CAGCGTC4250.09.8423922
AAACCGT1054.5103166E-49.0446576
CGCGGGG850.0074620658.93783812
TAGACTG6000.08.8730665
GTGGTAT24500.08.70181751
ACACCGC1107.172156E-48.6335366
GTCTTAG4300.08.6322541
CTAGGAC4200.08.6014413
CTACACT5500.08.4697454
TAATACT6750.08.450544
TGGTATC24850.08.4165332
CTAGACT3750.08.3660344
GACCGTG1606.4895685E-68.3092997
TATACTG9300.08.1779415
Produced by FastQC (version 0.11.5)