FastQCFastQC Report
Wed 25 May 2016
SRR1295279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104375
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48200.15526474733239382No Hit
GTGGTATCAACGCAGAGTACATGGG41720.1343909804711093No Hit
GGTATCAACGCAGAGTACTTTTTTT39970.12875377491443527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCT658.0436724E-411.6894826
GGTATCA28250.011.1426461
GTGGTAT12300.010.6697141
CTAGACT2100.010.4076144
TGGTATC12200.010.1255622
GTCTTAG3400.010.0693721
TTATACG1151.0238557E-59.9157614
TAGACAG4700.09.7047865
TAGGACG900.00111138349.5026034
GTCCTAG3600.09.2457971
CTACACT4350.09.1749274
TCGCCCA2800.09.158648512
CCAGTAC2700.09.1506563
GTATTAA4900.09.1218011
TAGACTG3550.08.8334065
CTAAGAC3250.08.7716343
CTTAGAC2607.2759576E-128.7716343
GTCGCCC2607.2759576E-128.76725311
GTCCTAA3150.08.7552041
GTCCTAC2201.5752448E-98.645421