##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295279_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3104375
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.65898449768472	31.0	30.0	33.0	25.0	34.0
2	29.468866357962554	31.0	30.0	33.0	23.0	34.0
3	29.94218095429837	31.0	30.0	34.0	25.0	34.0
4	33.18803076303604	35.0	33.0	37.0	26.0	37.0
5	33.493314757398835	35.0	33.0	37.0	27.0	37.0
6	33.5083200322126	35.0	33.0	37.0	27.0	37.0
7	33.480825609019526	35.0	33.0	37.0	27.0	37.0
8	33.43675200322126	35.0	33.0	37.0	27.0	37.0
9	34.40630014093014	37.0	34.0	39.0	25.0	39.0
10	34.32376275417757	37.0	33.0	39.0	25.0	39.0
11	34.725127602174354	38.0	34.0	39.0	27.0	39.0
12	34.72875087578015	38.0	34.0	39.0	27.0	39.0
13	34.622075941211996	38.0	34.0	39.0	26.0	39.0
14	34.715101107308236	38.0	33.0	40.0	19.0	41.0
15	35.34645814374874	38.0	34.0	40.0	25.0	41.0
16	35.41585149989933	38.0	34.0	40.0	25.0	41.0
17	35.22615212401852	38.0	33.0	40.0	25.0	41.0
18	35.20148210187236	38.0	33.0	40.0	25.0	41.0
19	35.28496782766257	39.0	34.0	40.0	25.0	41.0
20	35.27021381115362	39.0	34.0	40.0	25.0	41.0
21	35.252371894503725	39.0	34.0	40.0	25.0	41.0
22	34.95685347292128	38.0	33.0	40.0	23.0	41.0
23	34.63106164686934	38.0	32.0	40.0	21.0	41.0
24	34.98668234346688	38.0	33.0	40.0	24.0	41.0
25	33.52083430642239	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14703646953820027
1101	2	0.2920331116949093
1101	3	0.17488801679110466
1101	4	0.022201476150598864
1101	5	0.027982903086396504
1101	6	-0.03287373830666951
1101	7	0.031324284434916194
1101	8	0.09474371967766615
1101	9	-0.02134556217539796
1101	10	0.23395226817026327
1101	11	0.23547426727456156
1101	12	0.3679377031231823
1101	13	0.23561790777108627
1101	14	0.7797172682577411
1101	15	0.5151464741945446
1101	16	0.302472733135275
1101	17	0.5461880711968803
1101	18	0.6287994113286217
1101	19	0.5903365815289163
1101	20	0.6546128977031103
1101	21	0.6083889431572587
1101	22	0.30243868586796196
1101	23	-1.9038851354468918
1101	24	-0.25883881584269375
1101	25	0.1663663341907693
1102	1	0.15845133146988033
1102	2	0.05937331757046138
1102	3	0.16165451883031068
1102	4	0.26005815678428235
1102	5	-0.007468877455856671
1102	6	-0.09569638615926124
1102	7	-0.05847120512219561
1102	8	-0.08892356022025893
1102	9	0.19131867133265246
1102	10	0.7371641856023246
1102	11	0.3306146995072794
1102	12	0.2500928194207219
1102	13	0.24361650791154688
1102	14	0.6771484055511863
1102	15	0.43730118851576094
1102	16	0.29778303488716773
1102	17	0.5839766843505529
1102	18	0.45674230476112854
1102	19	0.5254801175712203
1102	20	0.5955512216850565
1102	21	0.5111001622513456
1102	22	0.44298959365746526
1102	23	0.45403938099342156
1102	24	0.5388157366715376
1102	25	0.2500533945459438
1103	1	-0.6707454812463034
1103	2	-1.8997653727615358
1103	3	-0.717319771134342
1103	4	-0.38417194939251686
1103	5	-0.6287020379649135
1103	6	-0.563752259911773
1103	7	-0.5033492340036858
1103	8	-0.1592636718359799
1103	9	0.36093057700995246
1103	10	0.4470154080582702
1103	11	0.08740836888767234
1103	12	-0.06044948499928893
1103	13	-0.20883924956513056
1103	14	-1.337483913929951
1103	15	-0.7128110896959825
1103	16	-0.5850149567133869
1103	17	-0.04694639516498711
1103	18	0.0971165609520881
1103	19	0.1105247529856257
1103	20	0.06682463933789506
1103	21	0.18869212894039578
1103	22	-0.3874263067968968
1103	23	0.6072847489519972
1103	24	0.4894811258496219
1103	25	0.12409914726490001
1104	1	-0.11566856680083504
1104	2	-0.05461528959419937
1104	3	-0.43981412499764616
1104	4	-0.27908279966801786
1104	5	-0.23844084418740152
1104	6	-0.19591719297590515
1104	7	-0.26628300094428425
1104	8	-0.18516213830593387
1104	9	-2.5300120103520705
1104	10	-1.0256073724032504
1104	11	-0.8427228028212568
1104	12	-0.2529901976711102
1104	13	-0.07677037032921419
1104	14	-1.8167635878629014
1104	15	-0.7497745313835438
1104	16	-0.31405675027254887
1104	17	0.05204477664914009
1104	18	0.0264736665434242
1104	19	0.008329240490233758
1104	20	0.24413487166292924
1104	21	0.3115090333121131
1104	22	0.532963353653443
1104	23	0.8888473230743728
1104	24	0.5216118201697739
1104	25	0.27209881063818386
1105	1	-0.30358701729903004
1105	2	-0.19023601190875183
1105	3	-1.6100448806784655
1105	4	-0.6524919027613549
1105	5	-0.6227050886725252
1105	6	-0.35610871074528916
1105	7	-0.2709149636312489
1105	8	-0.3942389368803987
1105	9	0.10384083599206662
1105	10	0.19880514390332849
1105	11	-0.5069205700641106
1105	12	-0.5439843694244075
1105	13	-0.48876665332211644
1105	14	0.3011515694078568
1105	15	0.08159026886561804
1105	16	-0.4861595398148921
1105	17	0.09048873944843194
1105	18	0.11163984688464268
1105	19	-0.012857444659353234
1105	20	-0.4320035255819974
1105	21	-0.10526625880226703
1105	22	-0.15881021526053019
1105	23	0.24276026443705234
1105	24	0.10430935637612748
1105	25	0.3008361770263335
1106	1	-1.8509985539648
1106	2	-1.2519507126449412
1106	3	-1.3903464318228416
1106	4	-1.8590714148276426
1106	5	-1.1892269692876951
1106	6	-1.0444182982229506
1106	7	-0.9854506342925049
1106	8	-1.113612442447824
1106	9	-0.5339704893898229
1106	10	-2.2228085367278823
1106	11	-1.7035669100896698
1106	12	-1.318105867087553
1106	13	-1.282239962225752
1106	14	-0.7536379248785181
1106	15	-1.5258174005505722
1106	16	-1.8421893836628982
1106	17	-1.0704954284090604
1106	18	-0.970009110608629
1106	19	-0.8240036125451411
1106	20	-0.6143330246788494
1106	21	-0.5834526130706266
1106	22	-0.5548547735215905
1106	23	-0.838423208258753
1106	24	-1.152949989870045
1106	25	-1.7564160949828
1107	1	-0.14376613858419773
1107	2	-0.025793991720949094
1107	3	-0.12284671529333124
1107	4	-1.4144909300028985E-4
1107	5	-0.2639381750835881
1107	6	-0.17483316074206812
1107	7	-0.3144624331178747
1107	8	-0.39121956433275784
1107	9	0.11617991129455874
1107	10	-0.18465986175652915
1107	11	-0.19041314186804925
1107	12	-0.40269263164295666
1107	13	-0.26439348984315103
1107	14	-1.4781708899614046
1107	15	-0.8479882037353974
1107	16	-0.46701162692390596
1107	17	-0.38066358402560496
1107	18	0.06681495055093478
1107	19	-0.05571810659871801
1107	20	0.02427832944354691
1107	21	0.11297197133397674
1107	22	-0.555348611380154
1107	23	0.3142128829274853
1107	24	0.089821881376281
1107	25	-0.13194876599386163
1108	1	-0.17773946545272423
1108	2	0.058693461302137706
1108	3	-0.21070404649276142
1108	4	-0.4355969492834362
1108	5	-0.46666611191133
1108	6	-0.4764934382587924
1108	7	-0.326411141556612
1108	8	-0.3260165104188957
1108	9	0.1651368330668319
1108	10	0.17067225330805513
1108	11	-0.12020122942757183
1108	12	-0.1893946680255354
1108	13	-0.28396225361266403
1108	14	-0.04675283672524699
1108	15	-0.12362376237311423
1108	16	-0.3410746240388889
1108	17	0.05153174334709831
1108	18	0.10650808784087218
1108	19	0.04750835777867479
1108	20	0.05059232276045833
1108	21	0.1464555277643882
1108	22	0.041554944812006056
1108	23	-0.06516973749004507
1108	24	-0.03406259300546566
1108	25	-0.8077752264434324
1109	1	-0.8608671481649104
1109	2	-0.8148822021287998
1109	3	-0.7459743457754691
1109	4	-0.7784764466605054
1109	5	-1.108799416819238
1109	6	-1.3306666628748829
1109	7	-0.9663139929905498
1109	8	-1.3445920846033346
1109	9	-1.0519453469431852
1109	10	-1.246114187162945
1109	11	-1.2345606906322857
1109	12	-1.2214420010315763
1109	13	-1.3738241350656253
1109	14	-0.7356877139729505
1109	15	-1.449311339911219
1109	16	-1.5815998988972453
1109	17	-1.6390657461072209
1109	18	-2.247691618796715
1109	19	-2.311497731501518
1109	20	-1.9504179142298668
1109	21	-1.6474294356687338
1109	22	-1.7918883201885336
1109	23	-1.4073203328642734
1109	24	-2.3704711771506553
1109	25	-1.3683700535162444
1110	1	-0.011717492218505043
1110	2	0.12112815657243914
1110	3	-0.09984794245244544
1110	4	0.28200740655006484
1110	5	0.14816884142912556
1110	6	0.14234317685810538
1110	7	0.0757562686040032
1110	8	0.04466823658110286
1110	9	0.28051462695257356
1110	10	0.21488765192233927
1110	11	0.18819887056175588
1110	12	0.22229004298858968
1110	13	0.14061102455946184
1110	14	-2.2926745443065712
1110	15	-0.5727434256995139
1110	16	-0.13169464786668073
1110	17	-0.4219323665229311
1110	18	-2.617499183876923
1110	19	-0.49015327343547455
1110	20	-2.0387005290304216
1110	21	-0.37603516410267446
1110	22	-1.7784994388462891
1110	23	-0.5762080842988055
1110	24	-0.0420039051791079
1110	25	-0.08145602270809604
1111	1	-0.10292709536638966
1111	2	-1.3556839291338605
1111	3	-0.36439283440989456
1111	4	0.12453413415853731
1111	5	0.02029322897330843
1111	6	0.21756410583215313
1111	7	0.19014938705407047
1111	8	0.2135226130298662
1111	9	-1.610982006241919
1111	10	-0.09917909455697327
1111	11	0.03376712730615594
1111	12	0.18063084630751547
1111	13	0.07509974886519899
1111	14	1.077811011397273
1111	15	0.4705296198700495
1111	16	0.49482117821080607
1111	17	-0.0322133409012082
1111	18	0.25931378162768226
1111	19	-0.02825111298284355
1111	20	0.41470618280143867
1111	21	-0.3444712379579258
1111	22	-0.14767783007035717
1111	23	0.08120244021370837
1111	24	-0.296719458087523
1111	25	-1.7334498946639094
1112	1	0.23383355812754303
1112	2	0.5167122996497575
1112	3	0.1277219501918161
1112	4	0.5775662824317678
1112	5	0.29101305225701424
1112	6	0.2915870842143704
1112	7	0.33485179584615565
1112	8	0.24658529386137218
1112	9	-0.2100718505218211
1112	10	0.5686416542195971
1112	11	0.2994869404663376
1112	12	0.23946676215265938
1112	13	0.2579635530032931
1112	14	0.608231957785101
1112	15	0.3453441062326448
1112	16	0.42411567971819863
1112	17	-3.3366280592950517
1112	18	-0.6893293113933652
1112	19	-1.0174872726025228
1112	20	0.14621986272385357
1112	21	-0.39278678425382907
1112	22	-1.6470357453526248
1112	23	-1.4859610891718589
1112	24	-0.3612025305375539
1112	25	0.1853171508251492
1113	1	-0.16842177143240278
1113	2	-0.03687206760173822
1113	3	-0.03987159646506555
1113	4	-0.425423140785405
1113	5	-0.11707409328196405
1113	6	-0.06764536975921231
1113	7	-0.11405982591153219
1113	8	-0.04129974949496784
1113	9	0.43754731472159136
1113	10	0.5576790634724063
1113	11	0.07414571218567545
1113	12	0.07260013406678212
1113	13	-0.3443896400276998
1113	14	-1.206463206912538
1113	15	-1.5764098141874143
1113	16	-0.3069097459643757
1113	17	0.021267005657122695
1113	18	-0.4239234602869857
1113	19	0.054189486234598405
1113	20	0.4422233397000568
1113	21	-1.4609218734836702
1113	22	-1.7578373083451808
1113	23	-0.4781649463100095
1113	24	-2.2009093400472466
1113	25	-0.4524203915784355
1114	1	0.3597734259420804
1114	2	0.43891513552321726
1114	3	0.1705446693911945
1114	4	0.5545540585770681
1114	5	0.40916214816941476
1114	6	0.4927835568954322
1114	7	0.42796368586740385
1114	8	0.35772527856427416
1114	9	0.8380483015229103
1114	10	0.6489578196176709
1114	11	0.5544120119835654
1114	12	0.5000517777003353
1114	13	0.5121329284592946
1114	14	1.4894700360878588
1114	15	0.8289783527952039
1114	16	0.7688644215784421
1114	17	0.04066529593670509
1114	18	0.2783537085844543
1114	19	-0.0661646921691883
1114	20	-1.2378050284147832
1114	21	-0.4540539826899703
1114	22	-1.2162159352288242
1114	23	-2.597623693086547
1114	24	-1.6369083364883537
1114	25	-1.1943283148775166
1115	1	0.6310228040886336
1115	2	0.7089790275683505
1115	3	0.5597177767554271
1115	4	0.8572742889583793
1115	5	0.7500233145502264
1115	6	0.7319851092722942
1115	7	0.7391200083453526
1115	8	0.8607355704466997
1115	9	1.0800841083044261
1115	10	1.26663245258829
1115	11	0.9517854449849636
1115	12	0.8644918079618336
1115	13	0.7830938863764203
1115	14	1.5183971390060194
1115	15	0.9897906846896163
1115	16	1.0418088785049306
1115	17	0.9661754708230745
1115	18	1.2633702288711248
1115	19	0.7096690461220518
1115	20	1.2520053581290895
1115	21	0.15874598466203338
1115	22	1.2557462427741868
1115	23	1.2656318935028423
1115	24	1.2021054455361266
1115	25	1.432226307642189
1116	1	0.8288405563856998
1116	2	1.0016271323925743
1116	3	0.8356308136224122
1116	4	1.21215710413896
1116	5	0.9724859657899074
1116	6	1.0430909968228832
1116	7	0.9648818681575264
1116	8	0.8713559791203593
1116	9	1.1911751820503795
1116	10	1.181295788182183
1116	11	1.0222578942493215
1116	12	1.0671028717389675
1116	13	0.44573371071955137
1116	14	1.6050277892470817
1116	15	1.385704688810904
1116	16	1.3189834851167248
1116	17	1.4892581887118084
1116	18	1.4463059023208444
1116	19	1.5815345158623515
1116	20	1.3286299368887526
1116	21	0.6809717675225855
1116	22	0.7970289831886177
1116	23	-0.19698649439357752
1116	24	0.32290304691973404
1116	25	-0.16366691358258834
1201	1	0.25429810999603575
1201	2	0.5457524302908716
1201	3	0.4701879816972543
1201	4	0.21008175546447916
1201	5	0.27067932194791666
1201	6	0.1304104119539531
1201	7	0.2724273565744255
1201	8	0.2352624623414954
1201	9	0.52349876605372
1201	10	0.7619282526786151
1201	11	0.42109685764275895
1201	12	0.4590575162261743
1201	13	0.449572026631472
1201	14	0.8681202632399092
1201	15	0.5849370256285198
1201	16	0.27190930645271294
1201	17	0.30854552738099983
1201	18	0.25462041703912064
1201	19	0.65194018007616
1201	20	0.7453893713761204
1201	21	0.6190796334007658
1201	22	0.8162642961493844
1201	23	0.34321302079042937
1201	24	0.6643234623529679
1201	25	0.7010608488348566
1202	1	0.31390766432459216
1202	2	0.6308595024287946
1202	3	0.40766652300267836
1202	4	0.36349711589497247
1202	5	0.11825538019895987
1202	6	0.014371238904566042
1202	7	0.04925072479590398
1202	8	0.06759178069459182
1202	9	0.5440335221209978
1202	10	-0.19176901291686477
1202	11	0.1031286694151845
1202	12	0.12641697932154017
1202	13	0.047212056085214726
1202	14	0.6132803154101794
1202	15	0.4767668503528242
1202	16	0.36717943227894523
1202	17	-1.7194209549478359
1202	18	-0.2075094407234701
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2210	18	0.8507993192070842
2210	19	0.9910711834176027
2210	20	1.143714293312712
2210	21	1.1161845103988028
2210	22	1.4061905694244317
2210	23	1.672845169867827
2210	24	1.2999234290085866
2210	25	1.3986892453963122
2211	1	0.2891915230041917
2211	2	-0.5181266813331362
2211	3	-0.01232563847863588
2211	4	0.40139085295369625
2211	5	0.4071669122770203
2211	6	0.3219524430938989
2211	7	0.3594072710635601
2211	8	0.40205392676531915
2211	9	0.9356540569626759
2211	10	0.9636047526372806
2211	11	0.6501562062168205
2211	12	0.428187085579367
2211	13	0.3554195987439641
2211	14	-0.674436224067982
2211	15	0.22712301319539563
2211	16	0.32418043148833675
2211	17	0.5575829955904794
2211	18	0.5011032744947386
2211	19	0.7302836775452093
2211	20	0.8485523701988029
2211	21	0.8749552341453892
2211	22	0.2343034854908126
2211	23	0.7304638787234836
2211	24	0.09914145416570364
2211	25	0.5944403072064688
2212	1	0.09435315639348474
2212	2	-0.9524960862290648
2212	3	-0.2967755261341445
2212	4	0.2389752948482311
2212	5	0.27018143731432076
2212	6	0.29076817790914333
2212	7	0.23961150603687997
2212	8	0.3693764786747451
2212	9	-2.065547289046833
2212	10	0.06828323519861357
2212	11	0.14473513901324964
2212	12	0.022236016160775307
2212	13	0.2151049029312304
2212	14	0.7048766903339541
2212	15	0.5255276750724533
2212	16	0.44538186116103873
2212	17	0.446743211134752
2212	18	0.26792743985290457
2212	19	-0.3701188367305406
2212	20	0.37563863593040736
2212	21	0.5242398748999548
2212	22	-1.4611513845127888
2212	23	-0.2552942674773675
2212	24	0.3796742230076262
2212	25	-0.2138410199525751
2213	1	0.353923706282373
2213	2	0.6767314815097336
2213	3	0.2737160542653534
2213	4	0.5735205178952185
2213	5	0.5194794425373459
2213	6	0.3450344564667631
2213	7	0.3990244238031977
2213	8	0.458177111245071
2213	9	0.8510569640199748
2213	10	0.9096972189520045
2213	11	0.21864216506992307
2213	12	0.3948788436335917
2213	13	0.31772601682090595
2213	14	-1.3075025130492506
2213	15	-0.21490824914842221
2213	16	0.17710143410054258
2213	17	-1.7625314325121977
2213	18	-0.6811667180639986
2213	19	-0.302618772300697
2213	20	0.4405008326365234
2213	21	0.05102237473384719
2213	22	0.6702731682247887
2213	23	1.1168982358604254
2213	24	0.8981926877289368
2213	25	-0.5139533997079155
2214	1	0.39129236426107994
2214	2	0.3849460488021883
2214	3	0.2900261925269376
2214	4	0.6151625784557524
2214	5	0.5402706640339261
2214	6	0.5555658357767541
2214	7	0.5060840990264737
2214	8	0.3811076737517425
2214	9	0.9591589658397197
2214	10	0.8827116980889258
2214	11	0.21098343990674095
2214	12	0.2207344793956736
2214	13	0.34491998594531736
2214	14	1.1123309210966212
2214	15	0.7769820985371325
2214	16	0.7753643624131001
2214	17	0.5805063017404919
2214	18	0.5521255652905381
2214	19	-0.5939828734161878
2214	20	0.2956053245771457
2214	21	0.6484295478786137
2214	22	0.9487364200927217
2214	23	1.542136313796881
2214	24	1.1975033244240905
2214	25	1.3205237124341807
2215	1	-0.008996797275869994
2215	2	0.3539945211298168
2215	3	0.28309474805438484
2215	4	0.6074718653545901
2215	5	0.6112445376039304
2215	6	0.6830502358104837
2215	7	0.515058998540205
2215	8	0.5714363973653107
2215	9	0.661044664465237
2215	10	0.961099420493845
2215	11	0.6963486337117288
2215	12	0.6255922526761637
2215	13	0.33981661860124035
2215	14	1.3080093088190239
2215	15	0.8587343542079324
2215	16	0.7910665717056204
2215	17	0.9057048148247873
2215	18	0.7457267683149666
2215	19	0.10732625888839209
2215	20	-1.0573439122893689
2215	21	-1.547386920626323
2215	22	0.12468366128787522
2215	23	0.8345295734871314
2215	24	1.0075737350826586
2215	25	0.39197296335471776
2216	1	0.16288473821736105
2216	2	-0.05823032800371308
2216	3	0.025638740505780078
2216	4	0.5000715745361433
2216	5	0.5573641710177739
2216	6	0.35732046107155213
2216	7	0.43210039615873086
2216	8	0.39319884164858365
2216	9	0.6979716535437035
2216	10	-1.223205537502956
2216	11	-0.170585467391696
2216	12	0.2295255193489325
2216	13	0.3298619841663708
2216	14	-0.026986254967390266
2216	15	-0.11536572817888668
2216	16	0.48723267973798556
2216	17	0.8524162954638825
2216	18	0.7624655527924418
2216	19	-0.9712283452675692
2216	20	-0.5783904009911041
2216	21	-0.5815418043341509
2216	22	-0.29449504066194265
2216	23	-3.995951790895006
2216	24	-1.6242918335624594
2216	25	-0.4512908575630874
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	12.0
4	38.0
5	68.0
6	76.0
7	259.0
8	184.0
9	569.0
10	1639.0
11	3705.0
12	7050.0
13	11362.0
14	15989.0
15	20088.0
16	23373.0
17	25605.0
18	26884.0
19	27504.0
20	28059.0
21	28852.0
22	30001.0
23	31167.0
24	33547.0
25	36050.0
26	39913.0
27	45518.0
28	52210.0
29	62808.0
30	75550.0
31	91742.0
32	117648.0
33	152441.0
34	199796.0
35	267140.0
36	363060.0
37	518441.0
38	766024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.69501000372037	21.298897495233486	13.65609382283969	23.34999867820646
2	15.889549342941473	21.330181454282666	40.1253062014842	22.654963001291662
3	17.93498277530432	26.392532302547565	30.572988983348225	25.099495938799887
4	12.729528495998427	17.65641742033511	38.01073423660758	31.60331984705888
5	13.24332181063805	37.48407115753133	35.61315327591108	13.659453755919534
6	30.405662501969367	38.57751836707867	17.60839258621806	13.408426544733901
7	25.503680500468157	32.91778013915211	24.20875522851452	17.369784131865217
8	23.792514310535726	37.14542675106692	21.320700362323045	17.741358576074305
9	24.631020150898113	17.31699881996052	22.264045273300624	35.78793575584074
10	15.291430044384299	29.92043047131131	33.769958837791904	21.01818064651249
11	31.787872490217023	25.837469652227007	23.801224704819074	18.5734331527369
12	20.836339044910243	27.663522254723468	31.55141756284931	19.948721137516984
13	26.175104308541265	24.476491791774084	27.061642328275546	22.2867615714091
14	21.31008816351837	23.555697630615512	28.0678948353818	27.066319370484315
15	22.686437884328903	30.936690277024546	24.464287843356377	21.912583995290174
16	22.068195995339583	31.252481002608594	25.734022343200635	20.94530065885119
17	20.361981036142684	30.253693467045238	28.465698411000428	20.918627085811647
18	20.379213958321586	30.014805745080192	29.301842260010798	20.304138036587428
19	21.065008724878922	28.786945416023364	29.70141453871552	20.446631320382195
20	22.241220219109515	28.478115467440933	28.959133150076227	20.321531163373322
21	22.67975441780867	28.861319902795486	27.94259353582878	20.516332143567062
22	21.74291404123812	28.329723218051285	29.415979681543046	20.51138305916755
23	22.09386470018146	28.596688386671065	28.642955089079514	20.666491824067965
24	21.609231324385107	27.925142890282174	29.013976506066797	21.45164927926593
25	22.305062772705725	28.22114438851032	28.416476666225932	21.057316172558025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2911.0
1	2911.0
2	3146.5
3	3382.0
4	3382.0
5	3382.0
6	4951.5
7	6521.0
8	6521.0
9	6521.0
10	9725.5
11	12930.0
12	12930.0
13	12930.0
14	21208.0
15	29486.0
16	29486.0
17	29486.0
18	46126.0
19	62766.0
20	62766.0
21	62766.0
22	89736.5
23	116707.0
24	116707.0
25	116707.0
26	151642.5
27	186578.0
28	186578.0
29	186578.0
30	224665.0
31	262752.0
32	262752.0
33	262752.0
34	295623.5
35	328495.0
36	328495.0
37	328495.0
38	351243.5
39	373992.0
40	373992.0
41	373992.0
42	373886.5
43	373781.0
44	373781.0
45	373781.0
46	359839.5
47	345898.0
48	345898.0
49	345898.0
50	328618.0
51	311338.0
52	311338.0
53	311338.0
54	282975.5
55	254613.0
56	254613.0
57	254613.0
58	220524.0
59	186435.0
60	186435.0
61	186435.0
62	154481.0
63	122527.0
64	122527.0
65	122527.0
66	95268.5
67	68010.0
68	68010.0
69	68010.0
70	50500.5
71	32991.0
72	32991.0
73	32991.0
74	23487.0
75	13983.0
76	13983.0
77	13983.0
78	9691.0
79	5399.0
80	5399.0
81	5399.0
82	3697.0
83	1995.0
84	1995.0
85	1995.0
86	1326.0
87	657.0
88	657.0
89	657.0
90	415.5
91	174.0
92	174.0
93	174.0
94	107.0
95	40.0
96	40.0
97	40.0
98	27.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08146567344473525
2	0.01990738876585464
3	0.031632776323736664
4	0.03929937588081337
5	0.04873766861284477
6	0.018941010670424804
7	0.02322528689349708
8	0.0343708475941212
9	0.03507952486410308
10	0.02577008254479565
11	0.024803704449365815
12	0.018103482987718945
13	0.02712301187839742
14	0.03749547010267767
15	0.034660761022750154
16	0.025480169116166698
17	0.02535131870344272
18	0.02738071270384538
19	0.0195530501308637
20	0.01736259311455607
21	0.013013891685121804
22	0.01919871149587276
23	0.02032615260720757
24	0.019166498892691765
25	0.11290517414938595
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3104375.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.58802354156748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.23873753326801	74.52638979758866
2	7.230721383721021	11.943419757225342
3	1.2736875658784936	3.1557401602612445
4	0.4507917577688684	1.4892000121183953
5	0.2286839644457938	0.94432783196141
6	0.1373995808694131	0.6808535891666755
7	0.0921394813857352	0.5326732360455043
8	0.06134166670459599	0.40528696111025275
9	0.043597326093215594	0.3240555294362295
>10	0.22319778861728265	3.238106910582767
>50	0.013223018683817887	0.7487662607256282
>100	0.005382748374937704	0.7593530750957007
>500	7.04689837557789E-4	0.3802007471747458
>1k	3.914943512294092E-4	0.8716261315073583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4820	0.15526474733239382	No Hit
GTGGTATCAACGCAGAGTACATGGG	4172	0.1343909804711093	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3997	0.12875377491443527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.442520636198912E-5	0.0
10	3.221260318099456E-5	0.0	0.0	1.2885041272397825E-4	0.0
11	3.221260318099456E-5	0.0	3.221260318099456E-5	1.2885041272397825E-4	0.0
12	3.221260318099456E-5	0.0	3.221260318099456E-5	1.610630159049728E-4	0.0
13	3.221260318099456E-5	0.0	3.221260318099456E-5	1.610630159049728E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCT	65	8.0436724E-4	11.689482	6
GGTATCA	2825	0.0	11.142646	1
GTGGTAT	1230	0.0	10.669714	1
CTAGACT	210	0.0	10.407614	4
TGGTATC	1220	0.0	10.125562	2
GTCTTAG	340	0.0	10.069372	1
TTATACG	115	1.0238557E-5	9.915761	4
TAGACAG	470	0.0	9.704786	5
TAGGACG	90	0.0011113834	9.502603	4
GTCCTAG	360	0.0	9.245797	1
CTACACT	435	0.0	9.174927	4
TCGCCCA	280	0.0	9.1586485	12
CCAGTAC	270	0.0	9.150656	3
GTATTAA	490	0.0	9.121801	1
TAGACTG	355	0.0	8.833406	5
CTAAGAC	325	0.0	8.771634	3
CTTAGAC	260	7.2759576E-12	8.771634	3
GTCGCCC	260	7.2759576E-12	8.767253	11
GTCCTAA	315	0.0	8.755204	1
GTCCTAC	220	1.5752448E-9	8.64542	1
>>END_MODULE