FastQCFastQC Report
Wed 25 May 2016
SRR1295279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104375
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT112110.36113549426213004No Hit
GGTATCAACGCAGAGTACTTTTTTT94730.3051499899335615No Hit
TATCAACGCAGAGTACTTTTTTTTT66380.21382725991544194No Hit
GTGGTATCAACGCAGAGTACATGGG50900.16396215019126234No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45810.14756593517213612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC550.00308021312.0853558
GTGCGGA550.00308021312.08535511
TAGACCG657.9673814E-411.7027685
TGGTCGG658.0583856E-411.68693510
AGCGTCA2850.011.3436563
GGTATCA33550.011.1550351
TGACCGT600.005896623711.0785976
ACCGTCC600.005898037511.078248
CACCGTC600.005898037511.078247
GTCCCCG700.001462408610.8833561
CAGCGTC3150.010.565172
GTGGTAT15300.09.7719031
TGGTATC15050.09.6034242
GCAGCGT3350.09.0965371
GACCGTG1054.5153513E-49.0434637
GCCCTAA1701.6252307E-78.9627641
TAATACT5550.08.7376814
GTCTCGC1651.0149342E-68.6572151
GTATCAA61150.08.5651921
TAAGACG1000.00288224038.5577884
Produced by FastQC (version 0.11.5)