FastQCFastQC Report
Wed 25 May 2016
SRR1295278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320147
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1699217.323716988621842No Hit
GCTTATGTACTCTGCGTTGATACCA981124.2286975782137946No Hit
CTTATGTACTCTGCGTTGATACCAC651712.808916848803115No Hit
GAGTACATAAGCAGTGGTATCAACG404481.7433378143712444No Hit
TATGTACTCTGCGTTGATACCACTG370141.5953299510763759No Hit
CCCATGTACTCTGCGTTGATACCAC306541.3212093888878593No Hit
GCGTTGATACCACTGCTTATGTACT287381.2386284144927024No Hit
CATAAGCAGTGGTATCAACGCAGAG258731.1151448593558944No Hit
CTGCTTATGTACTCTGCGTTGATAC220150.9488622919151244No Hit
ACTCTGCGTTGATACCACTGCTTAT212720.9168384589424723No Hit
ACGCAGAGTACATAAGCAGTGGTAT202140.8712379000123699No Hit
CATGTACTCTGCGTTGATACCACTG183530.7910274650701012No Hit
GTATCAACGCAGAGTACATAAGCAG173890.7494783735685714No Hit
TATCAACGCAGAGTACATAAGCAGT162050.698447124255489No Hit
GGTATCAACGCAGAGTACATAAGCA153600.6620270181156624No Hit
GTACATGATAAGCAGTGGTATCAAC149770.6455194433800961No Hit
ATCATGTACTCTGCGTTGATACCAC138110.5952640069788682No Hit
GTACATGGGAAGCAGTGGTATCAAC125040.5389313694347815No Hit
AAGCAGTGGTATCAACGCAGAGTAC115430.4975115800852274No Hit
GTGGTATCAACGCAGAGTACATAAG114770.49466693274176166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA103290.44518730925238786No Hit
GTACATGGGATAAGCAGTGGTATCA96310.41510300855937143No Hit
ACATAAGCAGTGGTATCAACGCAGA95040.40962921745906616No Hit
GCAGTGGTATCAACGCAGAGTACAT87310.3763123629666569No Hit
GTACTCTGCGTTGATACCACTGCTT81780.3524776662858No Hit
CCACTGCTTATGTACTCTGCGTTGA79310.3418317891064661No Hit
GTTGATACCACTGCTTATGTACTCT79200.34135768121588844No Hit
GATACCACTGCTTATGTACTCTGCG75760.32653103445600645No Hit
GCTTATCCCATGTACTCTGCGTTGA74580.3214451498116283No Hit
GTACTTTTTTTTTTTTTTTTTTTTT73310.31597135871132304No Hit
GTATCAACGCAGAGTACTTTTTTTT73160.31532484795144444No Hit
GTGGTATCAACGCAGAGTACATGGG65100.28058566978730226No Hit
ATACCACTGCTTATGTACTCTGCGT64470.27787032459581223No Hit
GGTATCAACGCAGAGTACTTTTTTT57450.24761362103349485No Hit
GTACTGGTTCACTATCGGTCAGTCA55250.23813146322194242No Hit
TATCAACGCAGAGTACTTTTTTTTT54970.23692464313683576No Hit
GAGTACATGATAAGCAGTGGTATCA53880.23222666494838473No Hit
CAGTGGTATCAACGCAGAGTACATA53840.23205426207908378No Hit
TACCACTGCTTATGTACTCTGCGTT53350.2299423269301471No Hit
GGATAAGCAGTGGTATCAACGCAGA53080.22877860756236568No Hit
GAGTACATGGGAAGCAGTGGTATCA51870.2235634207660118No Hit
TGATACCACTGCTTATGTACTCTGC51640.22257210426753132No Hit
GCAGAGTACATAAGCAGTGGTATCA48290.20813336396357643No Hit
GATAAGCAGTGGTATCAACGCAGAG47890.20640933527056693No Hit
GAGTACATGGGATAAGCAGTGGTAT43240.18636750171433103No Hit
GGGATAAGCAGTGGTATCAACGCAG42240.18205742998180718No Hit
GGATACCACGTGTCCCGCCCTACTC41930.1807213077447248No Hit
CCCCATGTACTCTGCGTTGATACCA41810.18020409913682192No Hit
GAACAAAAAAAAAAAAAAAAAAAAA41350.17822146613986095No Hit
GTACACAAGCAGTGGTATCAACGCA40620.17507511377511856No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA39750.17132535136782281No Hit
GCGTTGATACCACTGCTTCCCATGT39750.17132535136782281No Hit
GCTTATCATGTACTCTGCGTTGATA38570.16623946672344467No Hit
GCGTTGATACCACTGCTTATCATGT35300.1521455321580917No Hit
GTACATAAGCAGTTGTATCAACGCA33500.1443874030395488No Hit
GTATCAACGCAGAGTACATGGGAAG33010.1422754678906121No Hit
ATCAACGCAGAGTACATAAGCAGTG31640.13637066961705444No Hit
GGTATCAACGCAGAGTACATGGGAA31490.13572415885717584No Hit
TCCATGTACTCTGCGTTGATACCAC31430.13546555455322443No Hit
GCGTTGATACCACTGCTTATCCCAT31410.13537935311857396No Hit
TATCAACGCAGAGTACATGGGAAGC31240.1346466409240449No Hit
ACGCAGAGTACATGGGAAGCAGTGG31210.13451733877206917No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30090.12969005843164247No Hit
CAGTGGTATCAACGCAGAGTACATG29160.1256816917203953No Hit
ATGTACTCTGCGTTGATACCACTGC29040.12516448311249245No Hit
ATACAGGGTGACAGCCCCGTACACA29000.12499208024319149No Hit
ACGCAGAGTACATGATAAGCAGTGG28730.12382836087541005No Hit
TACATAAGCAGTGGTATCAACGCAG27750.11960449057753669No Hit
ACTCTGCGTTGATACCACTGCTTCC27690.11934588627358525No Hit
TATCAACGCAGAGTACATGATAAGC27300.11766495829790095No Hit
GTATCAACGCAGAGTACATGGGATA26790.11546682171431379No Hit
GTATTTAGCCTTGGAGGATGGTCCC26430.11391519589060521No Hit
ACGCAGAGTACATGGGATAAGCAGT25860.11145845500306663No Hit
AAAAAGTACTCTGCGTTGATACCAC25630.11046713850458613No Hit
GGTATCAACGCAGAGTACATGGGAT24740.10663117466263991No Hit
GGACATAAGCAGTGGTATCAACGCA23950.10322621799394609No Hit
GTATCAACGCAGAGTACATGATAAG23670.10201939790883939No Hit
CCCATATTCAGACAGGATACCACGT23590.10167459217023748No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23480.10120048427965987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATG250.00602453519.0045953
TCTAACC250.00602453519.0045953
AGCGTAA402.7649393E-416.62256613
TTGTATC5650.016.47546613
TAGATAG350.002168379316.2882462
GTTGCCT651.9176514E-716.0745711
GTTGTAT6150.015.44489312
GTACAAA7700.015.3109881
GTGTTAC508.657403E-515.2122071
GCAGTTG6500.015.1977769
AGTTGTA6150.015.13599511
AGCAGTT6900.015.005058
CAGTTGT6450.014.57926110
GTACGCT1850.014.3762736
TTCGTTG3900.014.36969213
CTTCGTT3850.014.30959312
CAAGTAA400.00525660714.25959819
TAGGGTC400.00527167814.2534464
TCGTTGA3950.013.94762514
TAGAACG759.694613E-713.931293510
Produced by FastQC (version 0.11.5)