##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295278_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2320147
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.77950966037928	31.0	30.0	33.0	25.0	34.0
2	29.446730745939806	31.0	30.0	34.0	22.0	34.0
3	29.94497633124108	31.0	30.0	34.0	25.0	34.0
4	32.866968773961304	35.0	33.0	37.0	23.0	37.0
5	33.69037953198655	35.0	33.0	37.0	28.0	37.0
6	33.631169921561	36.0	35.0	37.0	27.0	37.0
7	33.73662142959045	36.0	35.0	37.0	28.0	37.0
8	33.613278382792124	36.0	35.0	37.0	27.0	37.0
9	34.46817162878042	38.0	34.0	39.0	23.0	39.0
10	34.32505785193783	38.0	34.0	39.0	24.0	39.0
11	34.87874130389152	38.0	34.0	39.0	27.0	39.0
12	34.878475803472796	38.0	35.0	39.0	27.0	39.0
13	34.51572292617666	38.0	34.0	39.0	25.0	39.0
14	34.73424399402279	38.0	33.0	40.0	19.0	41.0
15	35.26480348012432	39.0	34.0	41.0	25.0	41.0
16	35.12768285802581	38.0	34.0	40.0	25.0	41.0
17	35.32946533129151	38.0	34.0	40.0	25.0	41.0
18	35.248256252728815	39.0	34.0	40.0	25.0	41.0
19	35.3260961482182	39.0	34.0	41.0	25.0	41.0
20	35.38855124265833	39.0	34.0	41.0	25.0	41.0
21	35.38858744726088	39.0	34.0	41.0	25.0	41.0
22	35.15959075006885	39.0	33.0	41.0	24.0	41.0
23	34.793772118749374	39.0	33.0	40.0	21.0	41.0
24	35.103806784656314	39.0	33.0	41.0	24.0	41.0
25	34.0047600432214	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4334444811912519
1101	2	0.6435371077941525
1101	3	0.3212145048230006
1101	4	0.27415112239260964
1101	5	0.23172092288171342
1101	6	0.11717282151267483
1101	7	0.07219045790279921
1101	8	0.23667777272600432
1101	9	0.1736065630888035
1101	10	0.29991464514640853
1101	11	0.42577521208243496
1101	12	0.4276989564499303
1101	13	0.44407573444624404
1101	14	0.9969802598899378
1101	15	0.7259009089711199
1101	16	0.5528335989133168
1101	17	0.9210751831588766
1101	18	0.8273632849398993
1101	19	0.7547734401403545
1101	20	0.8770659909125271
1101	21	0.8282867416554396
1101	22	0.6300809070140545
1101	23	-1.6313404874478863
1101	24	-0.02486471735330298
1101	25	0.462823640461977
1102	1	0.6120210272419442
1102	2	0.599819084513836
1102	3	0.555601226108223
1102	4	0.7455627760367989
1102	5	0.5728408771976703
1102	6	0.38375751820269244
1102	7	0.42937463116498975
1102	8	0.33515500656591257
1102	9	0.6336514674641833
1102	10	1.0449500882057166
1102	11	0.7461703537282389
1102	12	0.5837537772960104
1102	13	0.7396970837268455
1102	14	1.3460691077587157
1102	15	0.9890793211557778
1102	16	0.8693140946947082
1102	17	1.165971024394203
1102	18	1.1140769842512697
1102	19	1.0438988268110663
1102	20	1.0834285679305893
1102	21	1.1392532287888955
1102	22	1.0501965289322754
1102	23	1.1518167895474178
1102	24	1.2797122206360925
1102	25	0.9531621406431938
1103	1	-0.14822514823579525
1103	2	-1.1359836657142104
1103	3	-0.260979305286849
1103	4	0.10993585506046344
1103	5	-0.05542895860786956
1103	6	-0.0707146163833059
1103	7	-0.01311282620313392
1103	8	0.07827834704571046
1103	9	0.7236886087608951
1103	10	0.8275594785481104
1103	11	0.4913789884624009
1103	12	0.33502438149209013
1103	13	0.1423613217565105
1103	14	-0.8682312356203425
1103	15	-0.07145340298475844
1103	16	0.010302319000828675
1103	17	0.4562394454315424
1103	18	0.47466802763555194
1103	19	0.5332045445662672
1103	20	0.5491126315980637
1103	21	0.5305521138237239
1103	22	0.15983670718726017
1103	23	0.9452027118775135
1103	24	0.7842352302286244
1103	25	0.7087583913621955
1104	1	-0.031904765696417314
1104	2	-0.0049086234454698285
1104	3	-0.3579236093196627
1104	4	-0.2131445848249598
1104	5	-0.24104302465750038
1104	6	-0.17159855600436913
1104	7	-0.16308497386194176
1104	8	-0.18010531659387397
1104	9	-2.377120525354485
1104	10	-0.7602563749899716
1104	11	-0.6592754870856439
1104	12	-0.1032599869170383
1104	13	-0.08047048060588935
1104	14	-2.0459117420318265
1104	15	-0.6817764538292579
1104	16	-0.18215209321672177
1104	17	0.23778430985201027
1104	18	0.3444779417617383
1104	19	0.1791471810899452
1104	20	0.37816226033034894
1104	21	0.5414076273526263
1104	22	0.5184789945217432
1104	23	0.8789263048077416
1104	24	0.7554752367940836
1104	25	0.5426295254427131
1105	1	-0.19568713408524374
1105	2	-0.1603603565497025
1105	3	-1.4298648416536466
1105	4	-0.6097955842017058
1105	5	-0.4494569671809998
1105	6	-0.2170142920878888
1105	7	-0.3584589677201322
1105	8	-0.33962521100403364
1105	9	0.1635230757865429
1105	10	0.35756471097420217
1105	11	-0.1428504397656809
1105	12	-0.3409966243986986
1105	13	-0.3619518360701335
1105	14	0.4454027561745235
1105	15	0.15712823939329468
1105	16	-0.23633281055921174
1105	17	0.1460448933358407
1105	18	0.23035730274329325
1105	19	0.17388744804524947
1105	20	-0.5269299544566621
1105	21	0.013670711648529732
1105	22	0.07257881470498972
1105	23	0.2311771685820645
1105	24	0.10858159024254377
1105	25	0.4174500764046485
1106	1	-1.2226588051937242
1106	2	-0.8779203405999141
1106	3	-0.8234311468649018
1106	4	-1.1607546082877676
1106	5	-0.5123127234406653
1106	6	-0.4405391913049712
1106	7	-0.3453658056041746
1106	8	-0.33035870540201984
1106	9	-0.036678294203099426
1106	10	-1.7593343674476785
1106	11	-0.9079586727077853
1106	12	-0.6376794249495816
1106	13	-0.7377466815310996
1106	14	0.19436829178501114
1106	15	-0.5987833074384454
1106	16	-1.2739046081065837
1106	17	-0.1918011489075937
1106	18	-0.21792231756401037
1106	19	-0.009492712330022357
1106	20	0.0689394539127477
1106	21	0.10705658292528142
1106	22	0.02788905218135085
1106	23	-0.14547157024094304
1106	24	-0.5574118520131179
1106	25	-1.321973102961529
1107	1	0.10648299645447423
1107	2	0.21597385959223914
1107	3	0.04221148700023747
1107	4	0.10518606749945292
1107	5	-0.09980206700417682
1107	6	-0.03030275373477309
1107	7	-0.018179375476563564
1107	8	0.018988038126309448
1107	9	0.4968725657544084
1107	10	-0.13187915831088048
1107	11	-0.03143294164241439
1107	12	-0.10234318900592854
1107	13	0.023905201444307522
1107	14	-0.9807152682090319
1107	15	-0.5207350591536226
1107	16	-0.11444118505620082
1107	17	0.06354398431477648
1107	18	0.3668851561501114
1107	19	0.3195450622000777
1107	20	0.40910559283208414
1107	21	0.5073503961027299
1107	22	-2.760786052604658E-4
1107	23	0.5860310549637262
1107	24	0.6149069547366395
1107	25	0.42415928719260165
1108	1	-0.2180416274996233
1108	2	0.13025506661395525
1108	3	-0.21403306631297525
1108	4	-0.6228687050172468
1108	5	-0.4760850622130022
1108	6	-0.3385832768490573
1108	7	-0.37078127332733146
1108	8	-0.23729969888147906
1108	9	0.1215676621197801
1108	10	0.06838083045983012
1108	11	-0.3013661911153065
1108	12	-0.2654560286877228
1108	13	-0.3575512003740826
1108	14	-0.08430744842919324
1108	15	-0.26402565056540794
1108	16	-0.2881697233332332
1108	17	0.008695647743046209
1108	18	-0.03630212152074108
1108	19	0.03059914026182753
1108	20	0.2452593946557684
1108	21	0.05679221054552386
1108	22	0.004703192457029104
1108	23	-0.16475125778985955
1108	24	-0.06453169086372412
1108	25	-0.7552330410291574
1109	1	-0.8750262294361484
1109	2	-0.7055252588298337
1109	3	-0.8719926548014101
1109	4	-0.7110041272872891
1109	5	-1.0184364608144065
1109	6	-1.173941668457445
1109	7	-0.9360028306161965
1109	8	-1.104775492318808
1109	9	-0.8417543524041591
1109	10	-1.1123346079479148
1109	11	-1.2703128694369852
1109	12	-1.2068307597259107
1109	13	-1.2870462245806493
1109	14	-0.5607648852231648
1109	15	-1.3036218716520125
1109	16	-1.403648475350451
1109	17	-1.4888399896964373
1109	18	-1.9830107014023994
1109	19	-1.9923029576402698
1109	20	-1.7828958635475658
1109	21	-1.5045731068294046
1109	22	-1.5773267967844973
1109	23	-1.0569900286344023
1109	24	-2.0409992137254775
1109	25	-1.2080857171991468
1110	1	-0.07554111103839745
1110	2	0.13475355513042686
1110	3	-0.11089127369652729
1110	4	0.10902841389717821
1110	5	0.055196839765450534
1110	6	0.006300555624846993
1110	7	-0.08286903570147786
1110	8	-0.18748620634457325
1110	9	0.1618260118872783
1110	10	0.3001129258366859
1110	11	0.1480506850429606
1110	12	0.17613432446265165
1110	13	-0.04339569809885546
1110	14	-3.2002564026795532
1110	15	-1.0341164946231132
1110	16	-0.36989965811483927
1110	17	-0.30269091892466804
1110	18	-2.7402457318509974
1110	19	-0.4303206445170389
1110	20	-1.8430131240226189
1110	21	-0.4535053586915012
1110	22	-1.9759431140807564
1110	23	-0.49056378157614944
1110	24	0.0872702958215541
1110	25	0.023158293160662424
1111	1	-0.2546763490533728
1111	2	-1.3214493018920344
1111	3	-0.44663081269614935
1111	4	-0.177457476569586
1111	5	-0.10049985794358918
1111	6	0.06519110666216932
1111	7	-0.17884673013772812
1111	8	-0.09969176026139337
1111	9	-1.7961951418778384
1111	10	-0.40190398593834686
1111	11	-0.18588404168578876
1111	12	-0.1288552812570174
1111	13	-0.09037756429356136
1111	14	0.6351591578390341
1111	15	0.2645270517929674
1111	16	0.194581189721319
1111	17	-0.2643603246246613
1111	18	0.06030867658803629
1111	19	-0.162853196874714
1111	20	0.19845282066295766
1111	21	-0.5175904513270169
1111	22	-0.2943303853012438
1111	23	0.0460273158632063
1111	24	-0.7598060319072957
1111	25	-2.2874666980603884
1112	1	0.04150914946882267
1112	2	0.23510998659192595
1112	3	-0.14879423903634148
1112	4	0.25660716726566335
1112	5	-0.027232009032289284
1112	6	-0.04165248996464044
1112	7	-0.027735799797966365
1112	8	-0.14254948416930802
1112	9	-0.3995547774427948
1112	10	0.3831246211280046
1112	11	0.07979412203882674
1112	12	-0.05881881063687899
1112	13	-0.14154923380738182
1112	14	0.46281934385275747
1112	15	-0.003850344557264407
1112	16	-0.007925792572123669
1112	17	-3.6711666371602547
1112	18	-1.278640589932209
1112	19	-1.3705440236324904
1112	20	-0.17790648951356758
1112	21	-0.6406081578808767
1112	22	-1.8656355996508722
1112	23	-1.696457295975506
1112	24	-0.5287472992952402
1112	25	-0.08289295690688903
1113	1	-0.32892560626899225
1113	2	-0.17740484094126785
1113	3	-0.12244628383367129
1113	4	-0.46469272699403064
1113	5	-0.22386626286719036
1113	6	-0.2713390291612612
1113	7	-0.2612777573024161
1113	8	-0.27077622545663615
1113	9	0.23500976049906797
1113	10	0.2894340870667449
1113	11	-0.11282111723757282
1113	12	-0.09566873626550176
1113	13	-0.3665962612375395
1113	14	-1.5169598998859186
1113	15	-1.7074662631476087
1113	16	-0.6437602788695145
1113	17	-0.3121675190219477
1113	18	-0.7161302028686762
1113	19	-0.21234969517692548
1113	20	0.25727550742017513
1113	21	-1.5609101669467051
1113	22	-2.036344392092417
1113	23	-0.6997457104525822
1113	24	-2.3922959512621347
1113	25	-0.4814819781962143
1114	1	-0.04261665214573185
1114	2	0.08698557420872532
1114	3	-0.10495714896483577
1114	4	0.2560214298404091
1114	5	-0.053520029968986194
1114	6	-0.04662693552501196
1114	7	0.14403674313690118
1114	8	-0.009962085880886207
1114	9	0.3620759005141352
1114	10	0.29601788586177236
1114	11	0.10441188295070702
1114	12	0.10238063928363772
1114	13	0.17259960138327557
1114	14	0.7246912078083199
1114	15	0.24638144458428002
1114	16	0.1682121542922843
1114	17	-0.6300873367073976
1114	18	-0.4092863469630643
1114	19	-0.7330042310750073
1114	20	-1.7217698907849197
1114	21	-0.8673196137271759
1114	22	-1.5349519294339515
1114	23	-3.084564533409779
1114	24	-2.0512651758761677
1114	25	-1.4838981950909016
1115	1	0.11755302826135505
1115	2	0.22522261896196483
1115	3	0.16586268364514467
1115	4	0.4349115211621424
1115	5	0.39746529782384243
1115	6	0.07510748819306201
1115	7	0.2909819320949154
1115	8	0.2744531527882188
1115	9	0.5135635499576665
1115	10	0.7917947056408536
1115	11	0.39342234151106226
1115	12	0.36705189903850055
1115	13	0.0906635337034345
1115	14	1.0267122187235316
1115	15	0.45520625161868367
1115	16	0.4308032368039676
1115	17	0.3814757959909727
1115	18	0.5693565153229301
1115	19	-0.2031132150721433
1115	20	0.4904461440005221
1115	21	-0.5113468108159509
1115	22	0.42132254358782006
1115	23	0.5387172034748602
1115	24	0.5573585651221649
1115	25	0.6125156811894144
1116	1	0.033920673291785164
1116	2	0.21139476637988253
1116	3	0.15915668523249593
1116	4	0.5303286574503758
1116	5	0.34103823589842364
1116	6	0.2132240826599272
1116	7	0.17980491228875906
1116	8	0.1081010020998292
1116	9	0.27630616467019564
1116	10	0.2527317739627364
1116	11	0.12308850168523122
1116	12	0.09341562669826686
1116	13	-0.5563568379803243
1116	14	0.6185732634591048
1116	15	0.2776268210756996
1116	16	0.29343149118337664
1116	17	0.4152347092181188
1116	18	0.3767215967547628
1116	19	0.4883345508914019
1116	20	0.4239936678159566
1116	21	0.025116078863803182
1116	22	-0.1749677895408297
1116	23	-1.102482072162438
1116	24	-0.5400276352010707
1116	25	-1.2097650116574101
1201	1	0.47833027068463707
1201	2	0.7053008107678274
1201	3	0.5747808542036061
1201	4	0.27424780523546133
1201	5	0.4919560493179205
1201	6	0.2833628313680876
1201	7	0.30909850940044237
1201	8	0.23052827610055004
1201	9	0.5814935439643136
1201	10	0.9330219693395705
1201	11	0.47112674258223564
1201	12	0.6012602533722955
1201	13	0.4574660482808284
1201	14	1.215403435495091
1201	15	0.7271328355171107
1201	16	0.6562335602401745
1201	17	0.6406593325785721
1201	18	0.7001037133384571
1201	19	0.8876182652171849
1201	20	0.9714193490299365
1201	21	0.7863986400906384
1201	22	0.8877799281262568
1201	23	0.44145363631447054
1201	24	0.7947835754148187
1201	25	1.017403704251855
1202	1	0.490555515648218
1202	2	0.8311985836820384
1202	3	0.5665004478837474
1202	4	0.5344080015251791
1202	5	0.37204762540035574
1202	6	0.30789948205624285
1202	7	0.4858079576291985
1202	8	0.4799474750354662
1202	9	0.9435169296849537
1202	10	0.018729798286152288
1202	11	0.5080934551693659
1202	12	0.5735235449764673
1202	13	0.5073435457994577
1202	14	1.2865331076146234
1202	15	0.8723028279107439
1202	16	0.7696709538187889
1202	17	-1.0497913631238305
1202	18	0.3380696990911787
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2210	20	0.8658157081851954
2210	21	0.9278897050127313
2210	22	1.1429207108981316
2210	23	1.409203001950928
2210	24	1.1722794086037496
2210	25	1.0859706049389288
2211	1	0.06678273058289008
2211	2	-0.5970486405528206
2211	3	-0.07678650296895029
2211	4	0.2379517270677809
2211	5	0.08985423409278326
2211	6	0.1397750140746794
2211	7	0.10032659584739179
2211	8	0.17738181372592976
2211	9	0.6413771920984601
2211	10	0.6730533025708851
2211	11	0.31331546230980223
2211	12	0.10533457820840653
2211	13	-0.01804634357160495
2211	14	-1.0370425506205976
2211	15	-0.16765536760631505
2211	16	0.16935247657720254
2211	17	0.22247902505844763
2211	18	0.10990636190079073
2211	19	0.518441925748597
2211	20	0.5393718066095019
2211	21	0.4860965147793479
2211	22	-0.16147422657983412
2211	23	0.3499843288144362
2211	24	-0.30674320468216365
2211	25	0.16258152459047892
2212	1	-0.054953716927887086
2212	2	-1.1436851875563505
2212	3	-0.3125462633164702
2212	4	0.08948027278823645
2212	5	0.15837782692278068
2212	6	0.16860942660532885
2212	7	0.17082862647811226
2212	8	0.0585363645678143
2212	9	-2.2634930595692637
2212	10	-0.07545648088287749
2212	11	0.14237386944718367
2212	12	0.08323549865225743
2212	13	0.0836602561141504
2212	14	0.7861697060163308
2212	15	0.23352087591713655
2212	16	0.20974952332792185
2212	17	0.29474881895078653
2212	18	0.05485805626776141
2212	19	-0.5150278703184483
2212	20	0.28905701807236994
2212	21	0.34555050074163063
2212	22	-1.7668310684733441
2212	23	-0.4071136362054375
2212	24	0.2444566887572961
2212	25	-0.2768543025857184
2213	1	0.0451471019792713
2213	2	0.3145200518721225
2213	3	0.10489816798340001
2213	4	0.3741814308254874
2213	5	0.1575114678392211
2213	6	0.15919843510987164
2213	7	0.07566359631130837
2213	8	0.16174061815299723
2213	9	0.49695609063381596
2213	10	0.5535457142615172
2213	11	-0.07384206966713691
2213	12	-0.07150351576733982
2213	13	0.08349318853787935
2213	14	-1.990050702254237
2213	15	-0.6618087243562698
2213	16	-0.19169795751385266
2213	17	-2.3614622566699666
2213	18	-1.1090650742447465
2213	19	-0.5502949024043673
2213	20	0.31511837695875755
2213	21	-0.36743055792261714
2213	22	0.3090440317540555
2213	23	0.8799180553702044
2213	24	0.6745610567002984
2213	25	-0.8061042730104191
2214	1	0.15499551579393156
2214	2	0.10475602624311975
2214	3	0.19770641927266297
2214	4	0.5621201712536745
2214	5	0.38416875804081485
2214	6	0.41380384220472877
2214	7	0.2980733478349791
2214	8	0.24345963674965532
2214	9	0.6639478080896737
2214	10	0.616789971176658
2214	11	0.12554220912642933
2214	12	-0.08229582706598393
2214	13	-0.07869465331241088
2214	14	0.8293242000742538
2214	15	0.4401540823322776
2214	16	0.35565006561476054
2214	17	0.3944246006514902
2214	18	0.27544187996684855
2214	19	-0.8183242819949257
2214	20	0.1096581487981112
2214	21	0.5879780996801358
2214	22	0.6560107453030426
2214	23	1.0728044438684066
2214	24	0.8276631038951763
2214	25	0.8553240751689799
2215	1	-0.5024031513097498
2215	2	0.002695499639820298
2215	3	-0.10459774329698845
2215	4	0.19534643586636946
2215	5	0.014604680689124905
2215	6	0.026646625991467943
2215	7	0.11690651728761736
2215	8	-0.07480432042285656
2215	9	0.19211671445916778
2215	10	0.39576407802687186
2215	11	0.03501669836492738
2215	12	-0.07779566318528452
2215	13	-0.0909944457301961
2215	14	0.632538446769324
2215	15	0.15942535950915726
2215	16	0.1684240195096578
2215	17	0.2798709024104724
2215	18	0.22043805608397093
2215	19	-0.679732563432708
2215	20	-2.0033758291326222
2215	21	-2.1966640941361035
2215	22	-0.40980701936427977
2215	23	0.17271157814132465
2215	24	0.35297174587048374
2215	25	-0.38282844313103226
2216	1	-0.22948670910948366
2216	2	-0.5211401628054233
2216	3	-0.20883557187939772
2216	4	0.02784257019094838
2216	5	0.03960950975007904
2216	6	-0.12348790824924549
2216	7	-0.062269601234518746
2216	8	-0.19132422246329384
2216	9	0.20682351037965674
2216	10	-1.627756846124349
2216	11	-0.49465242117349817
2216	12	-0.28111927045013374
2216	13	-0.15369288616190602
2216	14	-0.702060149065936
2216	15	-0.6144966921671298
2216	16	-0.16531723600736115
2216	17	0.2208202229931473
2216	18	-0.046395225318754285
2216	19	-1.5705785566710517
2216	20	-1.2215588694705701
2216	21	-0.9362328371533764
2216	22	-0.9875602393044645
2216	23	-4.417778472051793
2216	24	-2.120265064483803
2216	25	-1.4541199427218956
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	13.0
4	30.0
5	57.0
6	56.0
7	301.0
8	254.0
9	687.0
10	1401.0
11	2577.0
12	4700.0
13	7727.0
14	11916.0
15	16607.0
16	22262.0
17	25987.0
18	25675.0
19	24193.0
20	23872.0
21	22873.0
22	21742.0
23	21805.0
24	22307.0
25	22934.0
26	24555.0
27	27739.0
28	31789.0
29	38549.0
30	47129.0
31	58283.0
32	77499.0
33	103694.0
34	139859.0
35	193882.0
36	275858.0
37	408854.0
38	612478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.87234946569818	15.945888703548656	10.953737265767112	20.228024564986054
2	10.939024311385765	24.364496194542816	39.49829611220441	25.19818338186701
3	20.744951922869642	33.215084258932706	31.440528964843462	14.59943485335419
4	12.227593718281609	21.31286224182253	30.346001574655123	36.11354246524074
5	7.970705144624375	50.072702434884754	31.541084637535672	10.415507782955205
6	27.823464746836805	29.231263337141378	28.33022223180221	14.615049684219603
7	21.74273307949121	41.24399884115553	18.63821117181249	18.375056907540767
8	19.922393947090075	39.495392490952504	26.363506613848443	14.218706948108984
9	29.20619644022909	24.431318877962415	15.032913090984668	31.32957159082383
10	13.358223916084821	28.457825767357843	27.891794945860827	30.29215537069651
11	26.188335973960186	31.02096334378806	19.69873142130393	23.091969260947824
12	27.638407372184798	27.915347136061747	21.94915758407131	22.497087907682147
13	28.32945597400761	20.74342688432765	34.30915459195464	16.617962549710104
14	32.21985825389078	25.97280123635448	21.34889067887383	20.45844983088092
15	34.98395330396362	21.801904044077858	22.91225865816592	20.301883993792604
16	24.752671177154564	23.467169192445038	32.65727750537512	19.122882125025274
17	18.843736904640778	36.16858458890703	26.903019757501973	18.084658748950215
18	19.826237372115113	22.6519172655	41.490904214978976	16.030941147405915
19	22.541592804375867	35.44907123198852	19.68622609103513	22.32310987260048
20	18.638174230928403	33.853505655952546	29.23722237550007	18.271097737618984
21	20.693360945230417	35.39863598722806	23.189294814621945	20.718708252919573
22	17.594226841101896	32.31075522016656	19.053855857269607	31.04116208146193
23	25.5670964429321	24.169299687572614	21.812966922014727	28.450636947480557
24	16.639211995614726	31.690464786528256	18.669923576714933	33.00039964114209
25	23.06166950419848	34.71956757913521	20.663430183342236	21.55533273332407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12114.0
1	12114.0
2	12522.0
3	12930.0
4	12930.0
5	12930.0
6	19065.5
7	25201.0
8	25201.0
9	25201.0
10	22361.0
11	19521.0
12	19521.0
13	19521.0
14	18643.0
15	17765.0
16	17765.0
17	17765.0
18	20808.0
19	23851.0
20	23851.0
21	23851.0
22	29422.0
23	34993.0
24	34993.0
25	34993.0
26	42728.5
27	50464.0
28	50464.0
29	50464.0
30	56452.5
31	62441.0
32	62441.0
33	62441.0
34	70773.5
35	79106.0
36	79106.0
37	79106.0
38	135095.5
39	191085.0
40	191085.0
41	191085.0
42	506486.0
43	821887.0
44	821887.0
45	821887.0
46	616978.0
47	412069.0
48	412069.0
49	412069.0
50	319999.5
51	227930.0
52	227930.0
53	227930.0
54	173744.5
55	119559.0
56	119559.0
57	119559.0
58	99776.0
59	79993.0
60	79993.0
61	79993.0
62	71794.0
63	63595.0
64	63595.0
65	63595.0
66	48365.0
67	33135.0
68	33135.0
69	33135.0
70	26276.5
71	19418.0
72	19418.0
73	19418.0
74	13962.0
75	8506.0
76	8506.0
77	8506.0
78	5869.5
79	3233.0
80	3233.0
81	3233.0
82	2056.5
83	880.0
84	880.0
85	880.0
86	590.0
87	300.0
88	300.0
89	300.0
90	203.0
91	106.0
92	106.0
93	106.0
94	76.5
95	47.0
96	47.0
97	47.0
98	32.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08137415431005018
2	0.01948152423100778
3	0.0322824372766036
4	0.03917855204864174
5	0.04762629264438848
6	0.021421056510643507
7	0.025644926808516877
8	0.04301451589058797
9	0.04348862378116559
10	0.02853267486930785
11	0.02680864617629831
12	0.02193826511854637
13	0.030127401410341672
14	0.04616086825533037
15	0.0421525015440832
16	0.02861887630395833
17	0.02685174689362355
18	0.03344615664438503
19	0.022196869422497798
20	0.020128034990886355
21	0.016119668279639177
22	0.025213919635264488
23	0.024481207440735436
24	0.02439500600608496
25	0.10365722516719846
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2320147.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.2973460889591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53902288807683	12.636802914067088
2	8.166692874082107	2.6619084034230434
3	3.4835742324254864	1.703190446772546
4	2.0992104164878445	1.368462346842015
5	1.3151446303346683	1.0716683598800134
6	0.9902599624610862	0.968316559576078
7	0.7708357368270298	0.8793803746565529
8	0.5851625395380016	0.7629277140116021
9	0.47915349513900024	0.7028037306013202
>10	3.753300623752836	12.183331960228221
>50	0.4540407426689214	5.080093464116553
>100	0.2875300722270809	9.113466855616577
>500	0.03245020241070658	3.6271023280537316
>1k	0.03245020241070658	10.635043932451884
>5k	0.005585690578892116	6.201702122134203
>10k+	0.005585690578892116	30.40379848756859
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	169921	7.323716988621842	No Hit
GCTTATGTACTCTGCGTTGATACCA	98112	4.2286975782137946	No Hit
CTTATGTACTCTGCGTTGATACCAC	65171	2.808916848803115	No Hit
GAGTACATAAGCAGTGGTATCAACG	40448	1.7433378143712444	No Hit
TATGTACTCTGCGTTGATACCACTG	37014	1.5953299510763759	No Hit
CCCATGTACTCTGCGTTGATACCAC	30654	1.3212093888878593	No Hit
GCGTTGATACCACTGCTTATGTACT	28738	1.2386284144927024	No Hit
CATAAGCAGTGGTATCAACGCAGAG	25873	1.1151448593558944	No Hit
CTGCTTATGTACTCTGCGTTGATAC	22015	0.9488622919151244	No Hit
ACTCTGCGTTGATACCACTGCTTAT	21272	0.9168384589424723	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	20214	0.8712379000123699	No Hit
CATGTACTCTGCGTTGATACCACTG	18353	0.7910274650701012	No Hit
GTATCAACGCAGAGTACATAAGCAG	17389	0.7494783735685714	No Hit
TATCAACGCAGAGTACATAAGCAGT	16205	0.698447124255489	No Hit
GGTATCAACGCAGAGTACATAAGCA	15360	0.6620270181156624	No Hit
GTACATGATAAGCAGTGGTATCAAC	14977	0.6455194433800961	No Hit
ATCATGTACTCTGCGTTGATACCAC	13811	0.5952640069788682	No Hit
GTACATGGGAAGCAGTGGTATCAAC	12504	0.5389313694347815	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	11543	0.4975115800852274	No Hit
GTGGTATCAACGCAGAGTACATAAG	11477	0.49466693274176166	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	10329	0.44518730925238786	No Hit
GTACATGGGATAAGCAGTGGTATCA	9631	0.41510300855937143	No Hit
ACATAAGCAGTGGTATCAACGCAGA	9504	0.40962921745906616	No Hit
GCAGTGGTATCAACGCAGAGTACAT	8731	0.3763123629666569	No Hit
GTACTCTGCGTTGATACCACTGCTT	8178	0.3524776662858	No Hit
CCACTGCTTATGTACTCTGCGTTGA	7931	0.3418317891064661	No Hit
GTTGATACCACTGCTTATGTACTCT	7920	0.34135768121588844	No Hit
GATACCACTGCTTATGTACTCTGCG	7576	0.32653103445600645	No Hit
GCTTATCCCATGTACTCTGCGTTGA	7458	0.3214451498116283	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7331	0.31597135871132304	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7316	0.31532484795144444	No Hit
GTGGTATCAACGCAGAGTACATGGG	6510	0.28058566978730226	No Hit
ATACCACTGCTTATGTACTCTGCGT	6447	0.27787032459581223	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5745	0.24761362103349485	No Hit
GTACTGGTTCACTATCGGTCAGTCA	5525	0.23813146322194242	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5497	0.23692464313683576	No Hit
GAGTACATGATAAGCAGTGGTATCA	5388	0.23222666494838473	No Hit
CAGTGGTATCAACGCAGAGTACATA	5384	0.23205426207908378	No Hit
TACCACTGCTTATGTACTCTGCGTT	5335	0.2299423269301471	No Hit
GGATAAGCAGTGGTATCAACGCAGA	5308	0.22877860756236568	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5187	0.2235634207660118	No Hit
TGATACCACTGCTTATGTACTCTGC	5164	0.22257210426753132	No Hit
GCAGAGTACATAAGCAGTGGTATCA	4829	0.20813336396357643	No Hit
GATAAGCAGTGGTATCAACGCAGAG	4789	0.20640933527056693	No Hit
GAGTACATGGGATAAGCAGTGGTAT	4324	0.18636750171433103	No Hit
GGGATAAGCAGTGGTATCAACGCAG	4224	0.18205742998180718	No Hit
GGATACCACGTGTCCCGCCCTACTC	4193	0.1807213077447248	No Hit
CCCCATGTACTCTGCGTTGATACCA	4181	0.18020409913682192	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	4135	0.17822146613986095	No Hit
GTACACAAGCAGTGGTATCAACGCA	4062	0.17507511377511856	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3975	0.17132535136782281	No Hit
GCGTTGATACCACTGCTTCCCATGT	3975	0.17132535136782281	No Hit
GCTTATCATGTACTCTGCGTTGATA	3857	0.16623946672344467	No Hit
GCGTTGATACCACTGCTTATCATGT	3530	0.1521455321580917	No Hit
GTACATAAGCAGTTGTATCAACGCA	3350	0.1443874030395488	No Hit
GTATCAACGCAGAGTACATGGGAAG	3301	0.1422754678906121	No Hit
ATCAACGCAGAGTACATAAGCAGTG	3164	0.13637066961705444	No Hit
GGTATCAACGCAGAGTACATGGGAA	3149	0.13572415885717584	No Hit
TCCATGTACTCTGCGTTGATACCAC	3143	0.13546555455322443	No Hit
GCGTTGATACCACTGCTTATCCCAT	3141	0.13537935311857396	No Hit
TATCAACGCAGAGTACATGGGAAGC	3124	0.1346466409240449	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3121	0.13451733877206917	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3009	0.12969005843164247	No Hit
CAGTGGTATCAACGCAGAGTACATG	2916	0.1256816917203953	No Hit
ATGTACTCTGCGTTGATACCACTGC	2904	0.12516448311249245	No Hit
ATACAGGGTGACAGCCCCGTACACA	2900	0.12499208024319149	No Hit
ACGCAGAGTACATGATAAGCAGTGG	2873	0.12382836087541005	No Hit
TACATAAGCAGTGGTATCAACGCAG	2775	0.11960449057753669	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2769	0.11934588627358525	No Hit
TATCAACGCAGAGTACATGATAAGC	2730	0.11766495829790095	No Hit
GTATCAACGCAGAGTACATGGGATA	2679	0.11546682171431379	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	2643	0.11391519589060521	No Hit
ACGCAGAGTACATGGGATAAGCAGT	2586	0.11145845500306663	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2563	0.11046713850458613	No Hit
GGTATCAACGCAGAGTACATGGGAT	2474	0.10663117466263991	No Hit
GGACATAAGCAGTGGTATCAACGCA	2395	0.10322621799394609	No Hit
GTATCAACGCAGAGTACATGATAAG	2367	0.10201939790883939	No Hit
CCCATATTCAGACAGGATACCACGT	2359	0.10167459217023748	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2348	0.10120048427965987	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.3100717325238445E-5	0.0
4	0.0	0.0	0.0	4.3100717325238445E-5	0.0
5	4.3100717325238445E-5	0.0	0.0	4.3100717325238445E-5	0.0
6	1.2930215197571533E-4	0.0	0.0	4.3100717325238445E-5	0.0
7	1.2930215197571533E-4	0.0	0.0	4.3100717325238445E-5	0.0
8	1.7240286930095378E-4	0.0	0.0	4.3100717325238445E-5	0.0
9	1.7240286930095378E-4	0.0	0.0	4.3100717325238445E-5	0.0
10	1.7240286930095378E-4	0.0	0.0	4.3100717325238445E-5	0.0
11	1.7240286930095378E-4	0.0	0.0	4.3100717325238445E-5	0.0
12	1.7240286930095378E-4	4.3100717325238445E-5	0.0	8.620143465047689E-5	0.0
13	1.7240286930095378E-4	4.3100717325238445E-5	0.0	8.620143465047689E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATG	25	0.006024535	19.004595	3
TCTAACC	25	0.006024535	19.004595	3
AGCGTAA	40	2.7649393E-4	16.622566	13
TTGTATC	565	0.0	16.475466	13
TAGATAG	35	0.0021683793	16.288246	2
GTTGCCT	65	1.9176514E-7	16.07457	11
GTTGTAT	615	0.0	15.444893	12
GTACAAA	770	0.0	15.310988	1
GTGTTAC	50	8.657403E-5	15.212207	1
GCAGTTG	650	0.0	15.197776	9
AGTTGTA	615	0.0	15.135995	11
AGCAGTT	690	0.0	15.00505	8
CAGTTGT	645	0.0	14.579261	10
GTACGCT	185	0.0	14.376273	6
TTCGTTG	390	0.0	14.369692	13
CTTCGTT	385	0.0	14.309593	12
CAAGTAA	40	0.005256607	14.259598	19
TAGGGTC	40	0.005271678	14.253446	4
TCGTTGA	395	0.0	13.947625	14
TAGAACG	75	9.694613E-7	13.9312935	10
>>END_MODULE