FastQCFastQC Report
Wed 25 May 2016
SRR1295278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320147
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1816877.830840028670597No Hit
GCTTATGTACTCTGCGTTGATACCA1122194.836719397520933No Hit
CTTATGTACTCTGCGTTGATACCAC772483.329444211940019No Hit
GAGTACATAAGCAGTGGTATCAACG433511.868459196766412No Hit
CCCATGTACTCTGCGTTGATACCAC375231.6172682161949221No Hit
TATGTACTCTGCGTTGATACCACTG342891.4778804963651009No Hit
GCGTTGATACCACTGCTTATGTACT328901.4175825928270924No Hit
CATAAGCAGTGGTATCAACGCAGAG324611.3990923850945651No Hit
CTGCTTATGTACTCTGCGTTGATAC260231.12160996695468No Hit
ACTCTGCGTTGATACCACTGCTTAT247391.0662686459090738No Hit
ACGCAGAGTACATAAGCAGTGGTAT243891.0511833948452403No Hit
CATGTACTCTGCGTTGATACCACTG211940.9134766029911037No Hit
GTATCAACGCAGAGTACATAAGCAG197070.8493858363284741No Hit
TATCAACGCAGAGTACATAAGCAGT184340.7945186231734455No Hit
GTATCAACGCAGAGTACTTTTTTTT181660.7829676309302815No Hit
GGTATCAACGCAGAGTACATAAGCA165280.712368655951541No Hit
ATCATGTACTCTGCGTTGATACCAC159920.6892666714652133No Hit
GTACATGATAAGCAGTGGTATCAAC150800.6499588172645957No Hit
GTACTTTTTTTTTTTTTTTTTTTTT144070.6209520345047103No Hit
GGTATCAACGCAGAGTACTTTTTTT140170.6041427547478673No Hit
AAGCAGTGGTATCAACGCAGAGTAC134310.5788857343952776No Hit
TATCAACGCAGAGTACTTTTTTTTT133410.575006669836006No Hit
GTACATGGGAAGCAGTGGTATCAAC128590.5542321240852411No Hit
ACATAAGCAGTGGTATCAACGCAGA124730.5375952471976991No Hit
GTGGTATCAACGCAGAGTACATAAG116990.5042352919879646No Hit
GCAGTGGTATCAACGCAGAGTACAT101530.4376015830031459No Hit
GTACATGGGATAAGCAGTGGTATCA96380.4154047135806482No Hit
CCACTGCTTATGTACTCTGCGTTGA93160.40152628260192136No Hit
GATACCACTGCTTATGTACTCTGCG87280.3761830608146811No Hit
GCTTATCCCATGTACTCTGCGTTGA84240.36308044274780865No Hit
GTACTCTGCGTTGATACCACTGCTT81810.35260696843777567No Hit
ACGCAGAGTACTTTTTTTTTTTTTT79090.3408835733253109No Hit
GTTGATACCACTGCTTATGTACTCT76790.330970408340506No Hit
ATACCACTGCTTATGTACTCTGCGT71890.3098510568511392No Hit
CAGTGGTATCAACGCAGAGTACATA67630.29149015127058764No Hit
GTGGTATCAACGCAGAGTACATGGG65840.28377512286936996No Hit
TACCACTGCTTATGTACTCTGCGTT63140.27213792919155555No Hit
GGATAAGCAGTGGTATCAACGCAGA61700.2659314258967212No Hit
GTACTGGTTCACTATCGGTCAGTCA57770.2489928439879025No Hit
GAGTACATGATAAGCAGTGGTATCA56290.2426139378237672No Hit
GCAGAGTACATAAGCAGTGGTATCA56230.24235533351981575No Hit
GAGTACATGGGAAGCAGTGGTATCA55860.24076060697878196No Hit
TGATACCACTGCTTATGTACTCTGC55370.23864867182984525No Hit
GATAAGCAGTGGTATCAACGCAGAG53530.23071813984200137No Hit
CCCCATGTACTCTGCGTTGATACCA51250.220891176291847No Hit
GGGATAAGCAGTGGTATCAACGCAG50260.21662420527664844No Hit
GAGTACTTTTTTTTTTTTTTTTTTT49880.21498637801828938No Hit
GGATACCACGTGTCCCGCCCTACTC47620.20524561590278545No Hit
GAGTACATGGGATAAGCAGTGGTAT46340.19972872408515496No Hit
GCTTATCATGTACTCTGCGTTGATA45470.19597896167785922No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43640.18809153040734056No Hit
GTACACAAGCAGTGGTATCAACGCA42850.18468657373864672No Hit
GCGTTGATACCACTGCTTCCCATGT42500.1831780486322634No Hit
GCGTTGATACCACTGCTTATCATGT42130.18158332209122957No Hit
ATCAACGCAGAGTACATAAGCAGTG37950.16356722224927991No Hit
TCCATGTACTCTGCGTTGATACCAC36910.15908474764745512No Hit
GTATCAACGCAGAGTACATGGGAAG36500.15731761823712032No Hit
GCGTTGATACCACTGCTTATCCCAT36300.15645560389061555No Hit
ACGCAGAGTACATGGGAAGCAGTGG36170.15589529456538745No Hit
CAGTGGTATCAACGCAGAGTACATG35690.15382646013377602No Hit
ATACAGGGTGACAGCCCCGTACACA35410.15261964004866935No Hit
TACATAAGCAGTGGTATCAACGCAG34590.14908538122799977No Hit
ACGCAGAGTACATGATAAGCAGTGG34510.14874057548939787No Hit
ACTCTGCGTTGATACCACTGCTTCC33320.1436115901276945No Hit
TATCAACGCAGAGTACATGGGAAGC33230.14322368367176735No Hit
ATGTACTCTGCGTTGATACCACTGC32740.14111174852283068No Hit
ACGCAGAGTACATGGGATAAGCAGT32040.138094698310064No Hit
GGTATCAACGCAGAGTACATGGGAA30770.1326209072097587No Hit
GCAGAGTACTTTTTTTTTTTTTTTT30570.13175889286325393No Hit
AAAAAGTACTCTGCGTTGATACCAC29870.12874184265048721No Hit
GTATCAACGCAGAGTACATGGGATA29460.12697471324015244No Hit
TATCAACGCAGAGTACATGATAAGC28690.12365595800610908No Hit
CTATCGGTCAGTCAGGAGTATTTAG27980.12059580707601716No Hit
CCCATATTCAGACAGGATACCACGT27740.11956138986021145No Hit
GTATTTAGCCTTGGAGGATGGTCCC26570.11451860593315855No Hit
ATGATAAGCAGTGGTATCAACGCAG26370.11365659158665377No Hit
GGTATCAACGCAGAGTACATGGGAT26300.11335488656537712No Hit
GTATCAACGCAGAGTACATGATAAG26110.11253597293619758No Hit
GCTTGTGTACTCTGCGTTGATACCA25570.11020853420063471No Hit
ACCCTGTATCGCGCGCCTTTCCAGA25180.1085276062249504No Hit
CTGCTTATCATGTACTCTGCGTTGA24640.10620016748938753No Hit
GCTTCCCATGTACTCTGCGTTGATA24640.10620016748938753No Hit
CCATGTACTCTGCGTTGATACCACT23990.10339862086324703No Hit
GTACATGGATAAGCAGTGGTATCAA23300.10042467136780557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC402.7694646E-416.61900315
TACCCGG402.7699198E-416.6186457
TCCCCAA402.7699198E-416.61864511
TAAAGGG350.002160256516.2974014
TCACACG350.002160256516.2974012
ACTAGTG702.4825567E-816.2974015
AAGCTCC350.002160256516.2974015
TAGACAG601.4566376E-615.8446955
ATTGCGT601.4734323E-615.8272828
TTATGTC851.0095391E-915.6582873
AGTACCG551.1211379E-515.5566115
GTACAAA4650.015.353121
TATATGG508.6637156E-515.2109072
AACCGTC508.745363E-515.194197
AATCTAA456.662755E-414.8072311
ACTGGCC456.78374E-414.7721298
GTCCTCT1101.2732926E-1114.6762077
GACCGTT653.3872657E-614.6097987
CTTGCAC400.005255071414.2602253
CCTAGTC602.5697218E-514.24977319
Produced by FastQC (version 0.11.5)