FastQCFastQC Report
Wed 25 May 2016
SRR1295277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2759289
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG43570.1579029960254254No Hit
GTATCAACGCAGAGTACTTTTTTTT31410.11383367236994747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG350.00217248916.2837879
TAGGACG653.3658707E-614.6189574
CAGTCCG551.9629873E-413.8165479
GTAACGG500.001497302113.3032493
GGACCGT550.003073037112.0890416
AGCGCTG951.3629286E-510.99869910
CTAGGAC3150.010.8597963
CGCCTCG700.001493961810.85546514
CAGTGCG909.531721E-510.5543069
GGTATCA26200.010.3426851
CCGTCCC750.00265128510.1321349
GTCTAGC1803.8926373E-1010.0362341
GTATTAG3400.09.5080121
ATTAGAC1502.6407906E-79.5023213
AAGACCG800.0045082269.5023215
AGTAACG800.00450890059.502152
CGTAGCA900.00111178759.5021492
GACCGTG800.0045230859.4985317
GTCCTAA1752.6353518E-89.2363541
CTTAGAC2401.0913936E-119.1063913
Produced by FastQC (version 0.11.5)