##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295277_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2759289 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.608221538229596 31.0 30.0 33.0 27.0 34.0 2 30.436089876776226 31.0 30.0 34.0 26.0 34.0 3 30.920313892455628 31.0 31.0 34.0 27.0 34.0 4 34.17361247770712 37.0 35.0 37.0 30.0 37.0 5 34.433531609048565 37.0 35.0 37.0 30.0 37.0 6 34.58567152625187 37.0 35.0 37.0 31.0 37.0 7 34.619246842211886 37.0 35.0 37.0 31.0 37.0 8 34.68782972715073 37.0 35.0 37.0 31.0 37.0 9 35.735828323890686 39.0 35.0 39.0 30.0 39.0 10 35.684182410758716 39.0 35.0 39.0 29.0 39.0 11 36.05188764207011 39.0 35.0 39.0 30.0 39.0 12 36.06428685070683 39.0 35.0 39.0 30.0 39.0 13 35.97846437977319 39.0 35.0 39.0 30.0 39.0 14 36.27699853114335 39.0 36.0 41.0 27.0 41.0 15 36.89130786952726 40.0 36.0 41.0 30.0 41.0 16 36.98576517356464 40.0 36.0 41.0 30.0 41.0 17 36.77905648882737 40.0 36.0 41.0 29.0 41.0 18 36.74794086447632 40.0 36.0 41.0 29.0 41.0 19 36.810983916508924 40.0 36.0 41.0 29.0 41.0 20 36.799021414574554 40.0 36.0 41.0 29.0 41.0 21 36.79469058877124 40.0 36.0 41.0 29.0 41.0 22 36.55135254045517 39.0 36.0 41.0 27.0 41.0 23 36.23445568767897 39.0 35.0 41.0 27.0 41.0 24 36.557795142154376 39.0 36.0 41.0 28.0 41.0 25 35.41587017525167 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5317568956780505 1101 2 0.7282762298916197 1101 3 0.5182511242299022 1101 4 0.6183796694874246 1101 5 0.43958868810061347 1101 6 0.3910837712239328 1101 7 0.42265082315453384 1101 8 0.4900419596833814 1101 9 0.49198502830147106 1101 10 0.7718442814004902 1101 11 0.6184607105081952 1101 12 0.6289006263865957 1101 13 0.495535613016834 1101 14 1.0804123597082196 1101 15 0.8216624556989487 1101 16 0.6008551807772449 1101 17 0.9908086567502394 1101 18 0.9836792879480996 1101 19 0.9046061170790267 1101 20 1.0799822440933085 1101 21 0.9905054098940553 1101 22 0.8404383285712456 1101 23 -1.3831279809398325 1101 24 0.2579676184988813 1101 25 0.7289783701805277 1102 1 0.3784861072000716 1102 2 0.5038236689479625 1102 3 0.4199836067720213 1102 4 0.446002829442115 1102 5 0.26384251720152463 1102 6 0.32835579707914775 1102 7 0.2565420399894549 1102 8 0.3121196210610009 1102 9 0.5566116353091246 1102 10 0.9853641718277544 1102 11 0.6706757135527468 1102 12 0.5885014950212977 1102 13 0.5465071651789231 1102 14 1.1756067653630993 1102 15 0.9174394683800031 1102 16 0.7141951264952979 1102 17 1.0077917622273844 1102 18 1.0078617259415523 1102 19 0.9892966106350372 1102 20 1.0682646316960671 1102 21 1.1216347122119785 1102 22 0.915090058866241 1102 23 0.9932305990411123 1102 24 1.055786869803299 1102 25 1.0319523991559478 1103 1 -0.2070521607619078 1103 2 -1.603699573509683 1103 3 -0.2543340174740045 1103 4 -0.03447538675013817 1103 5 -0.09919675203956757 1103 6 -0.02205345647352175 1103 7 0.08020831123780425 1103 8 0.1273859613043129 1103 9 0.7965776432423652 1103 10 0.9118918827964535 1103 11 0.5751601804300179 1103 12 0.46402041912302394 1103 13 0.31290691223657063 1103 14 -0.8955041798884622 1103 15 -0.08183596697438844 1103 16 0.126021591822834 1103 17 0.6286609753410133 1103 18 0.7295881750194226 1103 19 0.7568663336002786 1103 20 0.7436197074538882 1103 21 0.8171294998306138 1103 22 0.3157355790274323 1103 23 1.0986704169508315 1103 24 1.0037652556914196 1103 25 1.0453354237505366 1104 1 0.17881769599544128 1104 2 0.23122741441112638 1104 3 -0.07276033510126112 1104 4 0.020943163021705402 1104 5 0.15394554390584148 1104 6 0.08529620430677909 1104 7 0.20255842897999088 1104 8 0.20559958304019688 1104 9 -1.8714973476804815 1104 10 -0.21889694269724913 1104 11 -0.07982123810882769 1104 12 0.32642770428760315 1104 13 0.4851790608564741 1104 14 -1.2014240218193848 1104 15 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12 -0.018957823336876345 1107 13 0.17118211287451857 1107 14 -1.0635531365304445 1107 15 -0.4164229258388161 1107 16 -0.11280238311807267 1107 17 0.00769687289056975 1107 18 0.39707038240599957 1107 19 0.2419941976374531 1107 20 0.5192518528112728 1107 21 0.5262371332261679 1107 22 -0.18919379386089474 1107 23 0.5551168900731582 1107 24 0.5092028597306708 1107 25 0.06334096391674393 1108 1 -0.12292573473928314 1108 2 0.11430430365637534 1108 3 -0.095263895884937 1108 4 -0.3169900092728568 1108 5 -0.41016590101111916 1108 6 -0.35055894199829396 1108 7 -0.18228616522599594 1108 8 -0.27869194787687235 1108 9 0.2957211981665111 1108 10 0.25286333621107104 1108 11 -0.047736124383654044 1108 12 -0.20535377016440748 1108 13 -0.18776422710858753 1108 14 0.017882614940084807 1108 15 -0.11675897006757197 1108 16 -0.16591689617743555 1108 17 0.1285160567567445 1108 18 0.11607178496511494 1108 19 0.13058047812924656 1108 20 0.21603528643926495 1108 21 0.2146708728985658 1108 22 0.19972647053805304 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2213 7 0.028845648087163056 2213 8 0.05249199753422573 2213 9 0.43547641699398554 2213 10 0.4206614666461732 2213 11 -0.19453985003099916 2213 12 -0.19874175731114718 2213 13 -0.2054719128479121 2213 14 -2.053926541134132 2213 15 -0.7041566346095038 2213 16 -0.3302337484773119 2213 17 -2.367346706993466 2213 18 -1.0185511187028595 2213 19 -0.6384818182297423 2213 20 0.058745031995677266 2213 21 -0.314742566097145 2213 22 0.2778705004391071 2213 23 0.7065626245388117 2213 24 0.3961551699057182 2213 25 -1.1890607828081485 2214 1 0.04822095056462672 2214 2 0.07067750987884125 2214 3 0.0015147274714557568 2214 4 0.37943296097683543 2214 5 0.25148249004209333 2214 6 0.21167273842171852 2214 7 0.16335199285406787 2214 8 0.10532499846263477 2214 9 0.470943828712862 2214 10 0.5082039379168961 2214 11 -0.1666702651785812 2214 12 -0.21964498774432428 2214 13 -0.07442325768264624 2214 14 0.6965069420236958 2214 15 0.22398129695965707 2214 16 0.2358483506937077 2214 17 0.10557275797997079 2214 18 0.07942615371606365 2214 19 -1.1469020323256487 2214 20 -0.13534678541613232 2214 21 0.1838370085408414 2214 22 0.4145409660192101 2214 23 0.9394374716616198 2214 24 0.6886823583027493 2214 25 0.700491429962014 2215 1 -0.3415257565386476 2215 2 0.028054444682972246 2215 3 -0.04250135216669193 2215 4 0.2038948824282727 2215 5 0.14743452426773018 2215 6 0.19849833105406134 2215 7 0.0382136043508865 2215 8 7.586603063032271E-4 2215 9 0.11195818786535483 2215 10 0.38807930290775516 2215 11 0.06564012884083326 2215 12 0.014584809167118351 2215 13 -0.09218137135345472 2215 14 0.614295041725768 2215 15 0.07416752208943933 2215 16 0.068912848865601 2215 17 0.2959864316102241 2215 18 0.10664738382619277 2215 19 -0.5113175199623541 2215 20 -1.8011720618591411 2215 21 -2.1875288551830394 2215 22 -0.46773639883211615 2215 23 0.21728224793168494 2215 24 0.3116244926847074 2215 25 -0.37690429806765025 2216 1 -0.23026084484303055 2216 2 -0.6054582875291104 2216 3 -0.4049487761325814 2216 4 0.0493123053068274 2216 5 0.09512249233156922 2216 6 -0.09729814037068252 2216 7 -0.17342141635356967 2216 8 -0.27900984094803505 2216 9 0.04498969322749957 2216 10 -1.7925925659615132 2216 11 -0.7183491719316635 2216 12 -0.4576788106133165 2216 13 -0.41938677954246373 2216 14 -0.8913231473501924 2216 15 -0.8295453171051932 2216 16 -0.40006968096751905 2216 17 -0.10382855037326522 2216 18 -0.1493148010794414 2216 19 -1.5999940330124929 2216 20 -1.2754667931755677 2216 21 -1.2197316228637973 2216 22 -1.0128971171184702 2216 23 -4.815124462826969 2216 24 -2.4674635577445017 2216 25 -1.6275820122997047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 17.0 4 36.0 5 30.0 6 50.0 7 189.0 8 112.0 9 266.0 10 731.0 11 1732.0 12 3344.0 13 5345.0 14 7670.0 15 9746.0 16 11610.0 17 12630.0 18 13325.0 19 13800.0 20 13895.0 21 14091.0 22 14958.0 23 15819.0 24 17480.0 25 19549.0 26 22210.0 27 25974.0 28 30881.0 29 38388.0 30 48585.0 31 61107.0 32 83073.0 33 113522.0 34 158868.0 35 227159.0 36 333207.0 37 519494.0 38 920394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61459178151355 20.761340933854285 13.488953951463255 23.135113333168903 2 15.880401922616846 21.08382812443362 40.43150133756715 22.604268615382384 3 18.206493268730547 25.94512388544788 30.31335251115232 25.535030334669255 4 13.122214435320517 17.159785410230537 37.993896728764085 31.72410342568486 5 13.682968173536263 37.266060534641085 35.221661212687074 13.829310079135581 6 31.230442392935863 37.85276187030984 17.582880032419457 13.333915704334833 7 26.22237494961446 32.53445065986933 23.81380114313553 17.429373247380678 8 23.536466129569618 37.34051041741815 21.21280781783992 17.910215635172307 9 24.376173243841126 17.42553051104671 22.30840008149717 35.88989616361499 10 15.267886174883177 29.688076028409515 33.631413592068306 21.412624204639002 11 31.919516551218898 25.667429954699333 23.71033382281882 18.702719671262948 12 20.951849327978426 27.369981586463876 31.356403415928437 20.32176566962926 13 26.172245005377693 24.25447244035689 26.980149835950407 22.59313271831501 14 20.974940733444246 23.398497843418827 28.029682039777388 27.59687938335954 15 22.79112647610195 30.739065162924533 24.230168400605724 22.2396399603678 16 22.182347429072713 31.030092781393698 25.546764976156688 21.240794813376908 17 20.392727615450283 30.1094192640979 28.295056830230674 21.20279629022114 18 20.508294799238453 29.895570065352082 29.053366277628186 20.542768857781276 19 21.266291432689144 28.756906958502427 29.16614107811623 20.810660530692196 20 22.399749464276912 28.38223226169384 28.523738549734528 20.694279724294713 21 22.84382115406502 28.73248717459871 27.544329820679973 20.879361850656295 22 21.874534449217403 28.33977749255209 28.965731475201977 20.819956583028524 23 22.119467387372236 28.542274876775153 28.30814947777603 21.03010825807658 24 21.778718591871947 27.891758855171012 28.552435081411026 21.77708747154601 25 22.34239248351524 28.2081517113166 27.98911682900476 21.460338976163403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2195.0 1 2195.0 2 2425.0 3 2655.0 4 2655.0 5 2655.0 6 3729.0 7 4803.0 8 4803.0 9 4803.0 10 7836.5 11 10870.0 12 10870.0 13 10870.0 14 18462.0 15 26054.0 16 26054.0 17 26054.0 18 40420.5 19 54787.0 20 54787.0 21 54787.0 22 77416.0 23 100045.0 24 100045.0 25 100045.0 26 129993.0 27 159941.0 28 159941.0 29 159941.0 30 191877.5 31 223814.0 32 223814.0 33 223814.0 34 252896.0 35 281978.0 36 281978.0 37 281978.0 38 303526.0 39 325074.0 40 325074.0 41 325074.0 42 328078.5 43 331083.0 44 331083.0 45 331083.0 46 320752.5 47 310422.0 48 310422.0 49 310422.0 50 297065.5 51 283709.0 52 283709.0 53 283709.0 54 259462.0 55 235215.0 56 235215.0 57 235215.0 58 204922.0 59 174629.0 60 174629.0 61 174629.0 62 145244.0 63 115859.0 64 115859.0 65 115859.0 66 90187.5 67 64516.0 68 64516.0 69 64516.0 70 47645.0 71 30774.0 72 30774.0 73 30774.0 74 22112.0 75 13450.0 76 13450.0 77 13450.0 78 9211.5 79 4973.0 80 4973.0 81 4973.0 82 3356.5 83 1740.0 84 1740.0 85 1740.0 86 1145.0 87 550.0 88 550.0 89 550.0 90 331.0 91 112.0 92 112.0 93 112.0 94 71.0 95 30.0 96 30.0 97 30.0 98 20.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08281118795457816 2 0.019896429841165606 3 0.03239965077960301 4 0.03972037724210838 5 0.04943302423196701 6 0.016381031490358567 7 0.020331324482502557 8 0.031892273698043226 9 0.03301575152149702 10 0.023049415990858517 11 0.022107144267961783 12 0.016272307830024327 13 0.024354099914869372 14 0.035806325470075805 15 0.03355936982316821 16 0.023701757952863944 17 0.022976933550635688 18 0.025115165537209043 19 0.017287061993143886 20 0.014532729264676516 21 0.011271019454649369 22 0.01667096125124987 23 0.018519263476931922 24 0.016272307830024327 25 0.11260147088615945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2759289.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.74323010437463 #Duplication Level Percentage of deduplicated Percentage of total 1 88.2869062751335 70.40283082301328 2 8.42309392056032 13.43369433396001 3 1.6597789681759605 3.970684085449714 4 0.5998582637004002 1.9133854220908662 5 0.2881174399018598 1.1487707653588668 6 0.1641826416600032 0.7855472503822631 7 0.12589921579567326 0.7027727094608283 8 0.08638211730713895 0.5510711245861011 9 0.059310269448832414 0.4256633217789634 >10 0.28422837971705933 3.888120358024739 >50 0.015325245418548277 0.8425165070217758 >100 0.005868834757918458 0.836045542454627 >500 5.925899782747576E-4 0.31646336111490786 >1k 4.5583844461350375E-4 0.7824343953030303 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 4357 0.1579029960254254 No Hit GTATCAACGCAGAGTACTTTTTTTT 3141 0.11383367236994747 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.624122011141276E-5 0.0 6 0.0 0.0 0.0 3.624122011141276E-5 0.0 7 0.0 0.0 0.0 3.624122011141276E-5 0.0 8 0.0 0.0 0.0 3.624122011141276E-5 0.0 9 0.0 0.0 0.0 1.0872366033423828E-4 0.0 10 0.0 0.0 0.0 1.0872366033423828E-4 0.0 11 0.0 0.0 0.0 1.0872366033423828E-4 0.0 12 0.0 0.0 0.0 1.0872366033423828E-4 0.0 13 0.0 0.0 0.0 1.0872366033423828E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAATCG 35 0.002172489 16.283787 9 TAGGACG 65 3.3658707E-6 14.618957 4 CAGTCCG 55 1.9629873E-4 13.816547 9 GTAACGG 50 0.0014973021 13.303249 3 GGACCGT 55 0.0030730371 12.089041 6 AGCGCTG 95 1.3629286E-5 10.998699 10 CTAGGAC 315 0.0 10.859796 3 CGCCTCG 70 0.0014939618 10.855465 14 CAGTGCG 90 9.531721E-5 10.554306 9 GGTATCA 2620 0.0 10.342685 1 CCGTCCC 75 0.002651285 10.132134 9 GTCTAGC 180 3.8926373E-10 10.036234 1 GTATTAG 340 0.0 9.508012 1 ATTAGAC 150 2.6407906E-7 9.502321 3 AAGACCG 80 0.004508226 9.502321 5 AGTAACG 80 0.0045089005 9.50215 2 CGTAGCA 90 0.0011117875 9.502149 2 GACCGTG 80 0.004523085 9.498531 7 GTCCTAA 175 2.6353518E-8 9.236354 1 CTTAGAC 240 1.0913936E-11 9.106391 3 >>END_MODULE