FastQCFastQC Report
Wed 25 May 2016
SRR1295277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2759289
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105860.3836495560994154No Hit
GGTATCAACGCAGAGTACTTTTTTT87720.31790798281731275No Hit
TATCAACGCAGAGTACTTTTTTTTT63350.22958812940579984No Hit
GTGGTATCAACGCAGAGTACATGGG46310.16783309033595248No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43780.15866406164776506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2600.012.0650823
GGTATCA32800.010.9982251
TCAACGG700.001484430310.8637975
GCAGCGT2750.010.3835491
ACGGTGC750.00265594510.129998
GTGGTAT14650.010.0705061
TGGTATC14750.09.7958312
GTCTAGG2950.09.6795791
TCTAGTA1901.0404619E-99.5058222
TCATGCG1002.7657294E-49.4968649
CAGCGTC3350.09.3639452
CTAGGAC2900.09.1780353
CTGGACT4050.09.1537554
ACAGTCC2750.08.9788548
ACTGTTC3600.08.9692618
TCTGTCG1701.6945887E-78.9382268
ATACCGT850.0074579718.9382266
CTAATAC3200.08.9117093
GTTCTAC1502.6338384E-68.8837021
GCGTCAG3550.08.8363974
Produced by FastQC (version 0.11.5)