FastQCFastQC Report
Wed 25 May 2016
SRR1295276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059057
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63860.15732718215092817No Hit
GTGGTATCAACGCAGAGTACATGGG61320.1510695710851067No Hit
GGTATCAACGCAGAGTACTTTTTTT53160.13096637962955435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT500.001501821413.29818110
AAGGTCG855.3169104E-511.178665
TCGCACT750.00264484110.135324
TGTATCG750.00264484110.135325
AATACGC800.0045108029.5018615
TAGGACA4500.09.2907094
GGTATCA40300.08.9176931
TAGGACG1201.7612879E-48.7100394
TATACTG9650.08.664915
AGACGTA1000.002901348.5516755
ACAGCGC1000.0029100928.548838
GTCCAGA6150.08.502631
GTCCTGG7850.08.4780141
TAAGCTG3400.08.3839955
GTCCAAG5450.08.3735661
CGCAGAT1606.4675387E-68.3113628
CCTGGAC6300.08.2951743
TCTATAC5400.08.2700363
GTCTCGC2303.6452548E-98.2673791
ACTGTCC3800.08.2488718
Produced by FastQC (version 0.11.5)