FastQCFastQC Report
Wed 25 May 2016
SRR1295276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059057
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT156400.3853111695647536No Hit
GGTATCAACGCAGAGTACTTTTTTT128870.31748753466630303No Hit
TATCAACGCAGAGTACTTTTTTTTT93350.22997952480095746No Hit
GTGGTATCAACGCAGAGTACATGGG66740.1644224261940643No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64520.15895317557748018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3850.011.852893
CAGCGTC4200.010.8651492
CAACGCT700.001483152310.8651484
TAGACCG700.001483152310.8651485
AATCCGT803.7869267E-410.6833926
GCAGCGT3850.010.3840841
CTAGGAC4300.010.1702843
TAATCCG750.002632996310.1408045
ACCGATC750.002657608110.1294378
TCCGTCC951.6517015E-49.9961568
GGTATCA53850.09.9341381
AGCACCG1252.548688E-69.8872845
CCGATGG800.0045324489.4963489
CTGATCG800.0045324489.4963489
GCACCGA1106.870827E-59.4963476
CACCGAT900.00111825339.4963477
CGTGTTA1151.1161265E-49.08346410
CTAGAAC4650.08.7914243
TACACCG1856.505434E-88.7361665
CATGCGC1201.7724074E-48.70498610
Produced by FastQC (version 0.11.5)