FastQCFastQC Report
Wed 25 May 2016
SRR1295275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4739169
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG68950.14548964174942908No Hit
GTATCAACGCAGAGTACTTTTTTTT65430.13806217925547706No Hit
GGTATCAACGCAGAGTACTTTTTTT57670.12168800057562836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG853.9407314E-612.2961814
TGTACCG1604.5474735E-1111.2831435
CGTACAC1252.5683312E-69.881453
GTACGAC900.00111290549.5013943
GCGCGCA800.0045246729.49838513
GTCCTGG10150.09.0850421
GTTCTAG5350.09.06228351
TAATCGC1054.4882344E-49.0491384
TACGACA950.00182210929.0015114
TACGACC1401.0839371E-58.8229094
GGTATCA44200.08.8182591
GTCTAGA4550.08.7752431
GACTGCG1856.60184E-88.7283377
GTCTTAG4400.08.6422851
TCTATAC6300.08.59649853
TCCTATA4100.08.34262
GTATTAG7700.08.27191
GGACTGT7250.08.2538576
GTATAGA7500.08.2389781
GTATTGG6250.08.2136271
Produced by FastQC (version 0.11.5)