##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295275_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4739169
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.64131390967488	31.0	30.0	34.0	27.0	34.0
2	30.329915434541373	31.0	30.0	34.0	26.0	34.0
3	30.78093121389003	31.0	31.0	34.0	27.0	34.0
4	34.1500763108469	37.0	35.0	37.0	30.0	37.0
5	34.44056310294062	37.0	35.0	37.0	30.0	37.0
6	34.58710377283444	37.0	35.0	37.0	31.0	37.0
7	34.45736309466913	37.0	35.0	37.0	30.0	37.0
8	34.51215919921826	37.0	35.0	37.0	30.0	37.0
9	35.511974778700655	39.0	35.0	39.0	28.0	39.0
10	35.42412456698632	39.0	35.0	39.0	28.0	39.0
11	35.85732983989387	39.0	35.0	39.0	30.0	39.0
12	35.85202047025544	39.0	35.0	39.0	30.0	39.0
13	35.77946851019662	39.0	35.0	39.0	30.0	39.0
14	36.02600561406441	39.0	36.0	41.0	27.0	41.0
15	36.66078863193104	39.0	36.0	41.0	29.0	41.0
16	36.75147204077339	39.0	36.0	41.0	30.0	41.0
17	36.54892387251858	39.0	36.0	41.0	28.0	41.0
18	36.50721845960758	39.0	36.0	41.0	28.0	41.0
19	36.56688356123194	40.0	36.0	41.0	27.0	41.0
20	36.56113740615707	40.0	36.0	41.0	27.0	41.0
21	36.553797300750404	39.0	36.0	41.0	27.0	41.0
22	36.303200835420725	39.0	36.0	41.0	27.0	41.0
23	35.98995878813353	39.0	35.0	41.0	27.0	41.0
24	36.315692687895286	39.0	35.0	41.0	27.0	41.0
25	35.10737177762599	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.46004073754637886
1101	2	0.6673870583020438
1101	3	0.47053579240444776
1101	4	0.47681109329057136
1101	5	0.30809160336776387
1101	6	0.28347110924584484
1101	7	0.20130514622269402
1101	8	0.30636965137968275
1101	9	0.3417549030839311
1101	10	0.5773062203019705
1101	11	0.5151450600986038
1101	12	0.49006653154606994
1101	13	0.5170832436125821
1101	14	1.0497001053725015
1101	15	0.8138721287987138
1101	16	0.6432442648348697
1101	17	0.8644436040682848
1101	18	0.9564491812831122
1101	19	0.9052367098888965
1101	20	0.9684559692065164
1101	21	0.9345182659887215
1101	22	0.6917295319517223
1101	23	-1.3255016796971333
1101	24	0.29951444053375553
1101	25	0.7022224460472017
1102	1	0.41978265652091196
1102	2	0.45111586391334413
1102	3	0.39274759561145345
1102	4	0.5017798517725183
1102	5	0.25167100558025624
1102	6	0.2158045742531698
1102	7	0.28714921378139024
1102	8	0.2856240101304479
1102	9	0.5295175911463801
1102	10	0.9885053883956232
1102	11	0.6160879266289996
1102	12	0.5573224393295604
1102	13	0.5539456728611043
1102	14	1.18351224757793
1102	15	0.8587776961862943
1102	16	0.6943727482175532
1102	17	1.0166767515052655
1102	18	0.9513875740032773
1102	19	0.9906067976570654
1102	20	0.9961556431891623
1102	21	1.0251451428865863
1102	22	0.9083448178116171
1102	23	1.0482899629793963
1102	24	0.9740074024967456
1102	25	0.7862620519692953
1103	1	-0.10469196100988043
1103	2	-1.5732632397944748
1103	3	-0.2602095449593058
1103	4	0.1741129402693673
1103	5	0.14073825383391636
1103	6	0.186357280072599
1103	7	0.15864160503610947
1103	8	0.3551830335339048
1103	9	0.883335221580758
1103	10	1.022268678175159
1103	11	0.7282254193729543
1103	12	0.4902436396103198
1103	13	0.37031009440731566
1103	14	-0.7667858207725899
1103	15	0.09621145110999407
1103	16	0.2650479600788742
1103	17	0.7164149069132151
1103	18	0.840210299344406
1103	19	0.839153858691084
1103	20	0.8943957304472789
1103	21	0.9124574579108611
1103	22	0.5110581754981496
1103	23	1.2701710823236354
1103	24	1.1310019619089715
1103	25	1.0953765792517558
1104	1	0.16725699131200855
1104	2	0.215483424568351
1104	3	-0.15595260358269059
1104	4	0.021622486417577136
1104	5	0.03643956185933206
1104	6	0.09007750819787219
1104	7	0.08756724993875054
1104	8	0.16257152617929904
1104	9	-1.9805663544856884
1104	10	-0.4366489635433055
1104	11	-0.30380419291257965
1104	12	0.1085628508312837
1104	13	0.32851737326135577
1104	14	-1.4156907529953742
1104	15	-0.20016188593684348
1104	16	0.058778024031369114
1104	17	0.3845782832053928
1104	18	0.5178362240242649
1104	19	0.5946472347121627
1104	20	0.663768758539689
1104	21	0.8336681621329944
1104	22	0.7994990304285423
1104	23	1.208750342130024
1104	24	0.9200794093558713
1104	25	0.7120043543049732
1105	1	-0.09404676728471273
1105	2	0.04446400444080467
1105	3	-1.404858506532264
1105	4	-0.368028308840195
1105	5	-0.24295837138920007
1105	6	-0.06637498928415653
1105	7	-0.18241592699774856
1105	8	-0.036664516988331286
1105	9	0.4681222945830328
1105	10	0.5372509960743983
1105	11	0.020456257402202027
1105	12	0.012266794402620462
1105	13	-0.11263303290721893
1105	14	0.7386360322539005
1105	15	0.37574989768057065
1105	16	-0.10759793568037423
1105	17	0.3715221766309398
1105	18	0.3677213874726988
1105	19	0.2840347060249897
1105	20	-0.1781755391802946
1105	21	0.22176984425289703
1105	22	0.28715765598988696
1105	23	0.4346049683931952
1105	24	0.34761560474523634
1105	25	0.5937750083663644
1106	1	-1.3781786712887545
1106	2	-0.8705259875365101
1106	3	-0.7994036957250579
1106	4	-1.2055596234356258
1106	5	-0.7332459275457737
1106	6	-0.570993029622521
1106	7	-0.5331682178219381
1106	8	-0.5873237638337869
1106	9	-0.1515274239176705
1106	10	-1.664177322330005
1106	11	-1.0109175807411432
1106	12	-0.5823889153705011
1106	13	-0.7883732624313708
1106	14	-0.0348798589232473
1106	15	-0.7959060401770159
1106	16	-1.2552275898952132
1106	17	-0.5623853549850466
1106	18	-0.5014590323202555
1106	19	-0.3314629913375384
1106	20	-0.17793548863026842
1106	21	-0.1507059516882947
1106	22	-0.09320952849103747
1106	23	-0.3477049164375501
1106	24	-0.7099089091623867
1106	25	-1.1110151461710842
1107	1	0.0301865812203701
1107	2	0.1401393750846225
1107	3	0.031773305169810584
1107	4	0.15934039419560264
1107	5	-0.07914852998154487
1107	6	0.016363716650801052
1107	7	-0.07484211994533752
1107	8	-0.02072296477322766
1107	9	0.4187865117383254
1107	10	-0.04111481131048578
1107	11	0.015263994293036376
1107	12	-0.11613388797556468
1107	13	0.022037877843345655
1107	14	-1.0377421058070055
1107	15	-0.45019259087677455
1107	16	-0.19559265611211885
1107	17	-0.010927585102507464
1107	18	0.24277319423769939
1107	19	0.17098468882533524
1107	20	0.3056383279983095
1107	21	0.365596680021973
1107	22	-0.13905477953643697
1107	23	0.5432073499070924
1107	24	0.405917280196995
1107	25	0.07686215378933525
1108	1	-0.10739449695832448
1108	2	0.17957800266194113
1108	3	-0.12495591519101623
1108	4	-0.34124326130267946
1108	5	-0.3148575613389326
1108	6	-0.28088848480967954
1108	7	-0.16755582269448155
1108	8	-0.34144354037460545
1108	9	0.2017284146592857
1108	10	0.2134878519197514
1108	11	-0.004984341188063013
1108	12	-0.09502832526079175
1108	13	-0.1994550882219528
1108	14	0.0794091506362875
1108	15	-0.1452258812547811
1108	16	-0.21304298901589647
1108	17	0.08490547976003882
1108	18	0.18169367318958507
1108	19	0.051020395440453115
1108	20	0.2415492493900615
1108	21	0.1405719807092538
1108	22	0.10459508085875058
1108	23	-0.15624496968053592
1108	24	0.011577986812120855
1108	25	-0.724058164080084
1109	1	-0.6442732815604018
1109	2	-0.5539302333530536
1109	3	-0.6524032010906673
1109	4	-0.4895710078424713
1109	5	-0.7794869406641922
1109	6	-1.014019522151088
1109	7	-0.7084558371120124
1109	8	-1.0719744659801407
1109	9	-0.8385092943949246
1109	10	-0.9867075056350316
1109	11	-1.058263158229586
1109	12	-1.009436305364595
1109	13	-1.0414049692773162
1109	14	-0.4075643391272621
1109	15	-0.9388652266988373
1109	16	-1.1774916085372311
1109	17	-1.1039669791500017
1109	18	-1.7661158531944992
1109	19	-1.8883168486483584
1109	20	-1.7248695258406244
1109	21	-1.5015246888409166
1109	22	-1.5529679057329417
1109	23	-1.0794045717566618
1109	24	-1.8941224349006873
1109	25	-1.1314770654641677
1110	1	0.04128310232949772
1110	2	0.16363793291451856
1110	3	-0.01852110390203876
1110	4	0.2278926018116536
1110	5	0.10485639469532515
1110	6	0.1098085783934053
1110	7	0.016740113295085735
1110	8	0.009435654094794188
1110	9	0.27002331205652297
1110	10	0.21362646456358192
1110	11	0.16791135647330435
1110	12	0.2098585724358628
1110	13	0.14382929961007562
1110	14	-2.4650943467598765
1110	15	-0.6555672265860579
1110	16	-0.14731875800195127
1110	17	-0.23132387871872595
1110	18	-2.973411318870596
1110	19	-0.5444908172129956
1110	20	-1.9365298527154593
1110	21	-0.27700288615844926
1110	22	-1.786839790580359
1110	23	-0.41062750849742
1110	24	0.12106070567840987
1110	25	0.11133397469830442
1111	1	-0.255173799137296
1111	2	-1.3293885240471823
1111	3	-0.44513793744951613
1111	4	-0.01860277168027835
1111	5	-0.09316426030763836
1111	6	0.03263077046533169
1111	7	-0.08135066568340221
1111	8	-0.12706259992458513
1111	9	-1.6571046371425169
1111	10	-0.32481969905227004
1111	11	-0.13046205237711916
1111	12	-0.11627260707250286
1111	13	-0.12514031104944223
1111	14	0.6464277148793585
1111	15	0.11273560145998829
1111	16	0.1194900505278369
1111	17	-0.1842626248727086
1111	18	0.09856086372042938
1111	19	-0.1699237488383929
1111	20	0.2570776497252041
1111	21	-0.514543070047246
1111	22	-0.17562731221314465
1111	23	0.05981404687190661
1111	24	-0.47640932204279096
1111	25	-1.8189689765073425
1112	1	0.16266791451476337
1112	2	0.4139909954617522
1112	3	0.05187387572451385
1112	4	0.3281093252927363
1112	5	0.12143092774811493
1112	6	0.14701985898592085
1112	7	0.12178889037102181
1112	8	0.07435408710414748
1112	9	-0.2635353016468329
1112	10	0.5033751276515801
1112	11	0.15847989796336748
1112	12	0.07842185018345305
1112	13	0.03530832360524272
1112	14	0.5009731327914082
1112	15	0.2350558221103185
1112	16	0.18094606717991724
1112	17	-3.783665353841563
1112	18	-0.9494475795466997
1112	19	-1.0957350596951798
1112	20	-0.034768963278409615
1112	21	-0.4884893925845901
1112	22	-1.6027517474634223
1112	23	-1.4223238713029929
1112	24	-0.2658896002661706
1112	25	0.1310011489510785
1113	1	-0.38733066550469175
1113	2	-0.20675208035810755
1113	3	-0.24299221688274386
1113	4	-0.5736387030320031
1113	5	-0.3041579607750222
1113	6	-0.2297244418898785
1113	7	-0.4440008414914729
1113	8	-0.3865602383695901
1113	9	0.0962009240577828
1113	10	0.2005884158295288
1113	11	-0.2457767519023477
1113	12	-0.24874660533598103
1113	13	-0.6448566781342322
1113	14	-1.7982038271206022
1113	15	-2.021858869976583
1113	16	-0.6948030532389566
1113	17	-0.4944795061422198
1113	18	-0.77348626885302
1113	19	-0.2916223442212029
1113	20	0.013572079903845236
1113	21	-1.7745635142782135
1113	22	-2.121715813564805
1113	23	-0.916011573690561
1113	24	-2.6446847403182048
1113	25	-0.8827321929021394
1114	1	0.04843193376456867
1114	2	0.14455260131164138
1114	3	-0.0583876331342168
1114	4	0.25901241702287336
1114	5	-0.04364135083364573
1114	6	0.15041027528170758
1114	7	0.11684210063147304
1114	8	0.1041255984254974
1114	9	0.4141013118603354
1114	10	0.2120184539195904
1114	11	0.1304710966346576
1114	12	0.1327042248375605
1114	13	0.0809332700581109
1114	14	0.879077370710462
1114	15	0.325830922490816
1114	16	0.21155492432112766
1114	17	-0.41740148406464783
1114	18	-0.10394214175386196
1114	19	-0.510771766831084
1114	20	-1.657120141182432
1114	21	-0.7287562249706099
1114	22	-1.4809316241756747
1114	23	-2.8687257601895126
1114	24	-1.9645646897615237
1114	25	-1.5184993215671767
1115	1	0.2330486172436821
1115	2	0.32935340626530163
1115	3	0.18471182938733577
1115	4	0.3937336513770404
1115	5	0.26507983105867083
1115	6	0.11206974092125677
1115	7	0.22640428513444277
1115	8	0.2618496591542083
1115	9	0.5264780031273304
1115	10	0.7727526066618111
1115	11	0.3276867182990699
1115	12	0.20761671143728933
1115	13	0.24432450243589443
1115	14	0.8797776888294777
1115	15	0.40382967178376106
1115	16	0.401518304704652
1115	17	0.39007770965915256
1115	18	0.5620816742014298
1115	19	-0.0032946020677044885
1115	20	0.5443192086899273
1115	21	-0.43226294589458547
1115	22	0.5689393764217598
1115	23	0.6312002852427483
1115	24	0.5820934683422649
1115	25	0.7396128142333112
1116	1	0.1903757049856516
1116	2	0.43171023093812266
1116	3	0.22783581310529044
1116	4	0.5037634173377512
1116	5	0.38938233768907793
1116	6	0.25883059094697813
1116	7	0.2732021212401534
1116	8	0.21839594586060684
1116	9	0.4378228186881046
1116	10	0.4296013611365268
1116	11	0.24148305421763894
1116	12	0.2707164752101079
1116	13	-0.23891311843808438
1116	14	0.7445940332632688
1116	15	0.42539401228945195
1116	16	0.4135004875497472
1116	17	0.5741293802369754
1116	18	0.5907614368638221
1116	19	0.6663403601732085
1116	20	0.5188722779962731
1116	21	-0.024609663217333377
1116	22	-0.05657230398186641
1116	23	-0.8567498296177476
1116	24	-0.42282106761314253
1116	25	-1.0893401762752077
1201	1	0.5384371999066389
1201	2	0.6991718203026345
1201	3	0.6311421659952714
1201	4	0.49471289443674493
1201	5	0.5159031891672825
1201	6	0.32476993187444236
1201	7	0.3831443675722426
1201	8	0.4390360103166415
1201	9	0.7084452445363993
1201	10	1.0861762857977055
1201	11	0.7038252589899869
1201	12	0.66300160683668
1201	13	0.6929813186171785
1201	14	1.2658577197083503
1201	15	0.9233384341341306
1201	16	0.7009774537469866
1201	17	0.7695614613792259
1201	18	0.6736363029724401
1201	19	1.019911622660814
1201	20	1.0598520025844493
1201	21	1.0062264371399223
1201	22	1.103733807555443
1201	23	0.6586931499954858
1201	24	0.9666107270893107
1201	25	1.2158801737917244
1202	1	0.4399639223162133
1202	2	0.7740512793926193
1202	3	0.655250010306851
1202	4	0.5412058407599147
1202	5	0.394175711242859
1202	6	0.303896773310278
1202	7	0.27605267066730477
1202	8	0.387394919796094
1202	9	0.9125966430850809
1202	10	0.1601020648168614
1202	11	0.48289628203384183
1202	12	0.5051550406709282
1202	13	0.47738134936695786
1202	14	1.188170893464644
1202	15	0.8811825172753274
1202	16	0.7268998526744781
1202	17	-1.292965586207245
1202	18	0.17876259655373872
1202	19	0.6180369391222911
1202	20	0.7497842319054726
1202	21	0.8714908107234791
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2210	24	0.9813831972910023
2210	25	0.8327856181032303
2211	1	0.04326642095742628
2211	2	-0.6517795929307262
2211	3	-0.07920403146895794
2211	4	0.23739850039134325
2211	5	0.23722952395955588
2211	6	0.189271766164822
2211	7	0.2643971239609897
2211	8	0.3002113488229696
2211	9	0.7827210264846727
2211	10	0.7618079137279139
2211	11	0.42459963485858765
2211	12	0.3431477351152239
2211	13	0.24041182974225705
2211	14	-0.7296039613883067
2211	15	0.050358240832480305
2211	16	0.2960989012207875
2211	17	0.40881466739732986
2211	18	0.314507636960748
2211	19	0.6020493592654503
2211	20	0.6855337387997622
2211	21	0.6072446370602052
2211	22	0.04960619542458744
2211	23	0.587218736870696
2211	24	0.012117411721668248
2211	25	0.38804550725988207
2212	1	-0.14375419764465036
2212	2	-1.370015220414654
2212	3	-0.546414473592133
2212	4	0.004257104101490938
2212	5	-0.03569811008067347
2212	6	-0.019796151412798224
2212	7	-0.005194305578740455
2212	8	0.021722925054980635
2212	9	-2.458420950914345
2212	10	-0.16725111181520447
2212	11	-0.15708839399690788
2212	12	-0.13651718837721205
2212	13	-0.0066628840404519
2212	14	0.45305510312315533
2212	15	0.18345066721158076
2212	16	0.17966514572821524
2212	17	0.2867676508962873
2212	18	-0.0047980893294763405
2212	19	-0.6505735478530568
2212	20	0.1590206657813482
2212	21	0.20547332158368192
2212	22	-1.9043579170441447
2212	23	-0.5476261974385395
2212	24	0.06997334617917517
2212	25	-0.5379681475574642
2213	1	0.08761540966440151
2213	2	0.4591362751823418
2213	3	0.14421756840911115
2213	4	0.31483459886681686
2213	5	0.258307996963012
2213	6	0.15087229477286712
2213	7	0.15552268483847342
2213	8	0.15969523081183468
2213	9	0.6153814082248843
2213	10	0.605905852547501
2213	11	0.019033579009679613
2213	12	0.03780428224077781
2213	13	0.059246800902343466
2213	14	-1.7084858882055158
2213	15	-0.4744857290262843
2213	16	-0.15784814242142176
2213	17	-2.1831444358865397
2213	18	-0.9522195899146197
2213	19	-0.5397171575256507
2213	20	0.16577462015277433
2213	21	-0.19298008300700076
2213	22	0.28281694016509107
2213	23	0.7155515620206572
2213	24	0.5428488299539893
2213	25	-1.0882776006776993
2214	1	0.05865772338539088
2214	2	0.10178694931061472
2214	3	0.008677144509320556
2214	4	0.32749462103384275
2214	5	0.24755427054157053
2214	6	0.210687622518563
2214	7	0.23560220197772708
2214	8	0.11261616771572847
2214	9	0.5039642366698729
2214	10	0.5039435277687048
2214	11	-0.17015056644864046
2214	12	-0.1407170544765748
2214	13	-0.09150061767659423
2214	14	0.7269261121337891
2214	15	0.2396182500476982
2214	16	0.28615551640764636
2214	17	0.1991390326977438
2214	18	0.1502036949819896
2214	19	-1.1270780264843125
2214	20	-0.10951321118714219
2214	21	0.28117003102567395
2214	22	0.4819143531785173
2214	23	0.843465369691593
2214	24	0.6935235891666309
2214	25	0.64584757019216
2215	1	-0.2558893355237615
2215	2	0.17831682726311016
2215	3	0.09205161880738189
2215	4	0.18526421294797046
2215	5	0.2289266008819837
2215	6	0.14338906532702822
2215	7	0.09679912005046987
2215	8	0.10248666654533878
2215	9	0.22035194859086715
2215	10	0.5304031463155425
2215	11	0.2194984071312902
2215	12	0.053905562891422676
2215	13	0.008961875898563676
2215	14	0.8617803042946477
2215	15	0.2339444473706891
2215	16	0.29197853623491454
2215	17	0.45573185858388854
2215	18	0.24706045691044665
2215	19	-0.43997521727592215
2215	20	-1.8301040896739167
2215	21	-1.9804648161946545
2215	22	-0.23412179768756403
2215	23	0.3522927908967475
2215	24	0.497336388116544
2215	25	-0.15107243415532423
2216	1	-0.1995739031004078
2216	2	-0.3852629851484508
2216	3	-0.2637545939650643
2216	4	0.09790133354191966
2216	5	0.08589882895460477
2216	6	-0.05280440375804574
2216	7	0.04369669539465093
2216	8	-0.0967754656336055
2216	9	0.25442418324175975
2216	10	-1.709477634842294
2216	11	-0.5481483073326103
2216	12	-0.2293859812795347
2216	13	-0.1687390901336414
2216	14	-0.5839399978384705
2216	15	-0.6037357377750894
2216	16	-0.09554528869933421
2216	17	0.1776111793776991
2216	18	0.00744775095228789
2216	19	-1.4327206499262601
2216	20	-1.0338258302637797
2216	21	-0.9847340442044086
2216	22	-0.8675818971259517
2216	23	-4.569187433913392
2216	24	-2.196651732292473
2216	25	-1.222728458850682
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	17.0
4	54.0
5	92.0
6	125.0
7	495.0
8	239.0
9	577.0
10	1581.0
11	3336.0
12	5962.0
13	9574.0
14	13894.0
15	17936.0
16	21111.0
17	23224.0
18	24701.0
19	25939.0
20	26276.0
21	27360.0
22	28614.0
23	30367.0
24	33190.0
25	36991.0
26	41712.0
27	48933.0
28	57858.0
29	71735.0
30	89194.0
31	112419.0
32	150148.0
33	203123.0
34	279847.0
35	391937.0
36	566566.0
37	861573.0
38	1532469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97505756280063	20.18610159562415	13.345256641500166	23.493584200075055
2	16.212292356279775	20.449060015651632	39.97468217647173	23.36396545159686
3	18.43087606190393	25.310943370259913	30.13600349037561	26.122177077460545
4	13.001618452597196	17.03869404153177	37.53062172686592	32.42906577900512
5	13.545760716669028	37.70754240782232	35.069581741102596	13.677115134406053
6	31.807168702059368	37.62317038372767	17.26979210460112	13.299868809611848
7	27.12381295709581	32.395757724524785	22.802853314180098	17.677576004199302
8	24.24317395768597	37.0213157610311	20.57917921991948	18.156331061363453
9	24.981328625064858	17.08788613015542	21.477548542408403	36.45323670237131
10	15.34324584167496	29.69529009864314	33.44508034872836	21.516383710953534
11	32.55403063149923	25.05268192359939	23.583371254750027	18.809916190151352
12	21.467127945889178	26.93920468168019	30.73842665288907	20.855240719541555
13	26.84903795780852	23.552968571051057	26.635117125613217	22.96287634552721
14	21.50513800349331	23.008561861443845	27.520416697804162	27.965883437258682
15	23.12597341133149	30.58192951710354	23.871455030665757	22.420642040899214
16	22.51305452718761	30.380765837452667	25.488135893723275	21.61804374163645
17	20.96330171632666	29.790278335051063	27.705451268940283	21.540968679681992
18	21.135061532067535	29.28585736566383	28.586707519887135	20.9923735823815
19	21.776864239778256	28.507394613591785	28.767582692765114	20.948158453864842
20	22.641677875139475	28.116315983750052	28.302229249692555	20.939776891417917
21	23.009600717204727	28.206528116265506	27.53602695933392	21.247844207195854
22	22.443173783600905	27.75433396327006	28.446476413674677	21.356015839454358
23	22.68085407254741	27.959003842334486	27.932241523594847	21.42790056152326
24	22.18393650226812	27.667701056487477	28.103976755038296	22.044385686206102
25	22.678579387733848	27.796041687482454	27.889514694484795	21.6358642302989
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3477.0
1	3477.0
2	3914.5
3	4352.0
4	4352.0
5	4352.0
6	6281.5
7	8211.0
8	8211.0
9	8211.0
10	12525.0
11	16839.0
12	16839.0
13	16839.0
14	28345.5
15	39852.0
16	39852.0
17	39852.0
18	62781.5
19	85711.0
20	85711.0
21	85711.0
22	122358.0
23	159005.0
24	159005.0
25	159005.0
26	207971.0
27	256937.0
28	256937.0
29	256937.0
30	309999.0
31	363061.0
32	363061.0
33	363061.0
34	410797.0
35	458533.0
36	458533.0
37	458533.0
38	496252.0
39	533971.0
40	533971.0
41	533971.0
42	549884.0
43	565797.0
44	565797.0
45	565797.0
46	553842.5
47	541888.0
48	541888.0
49	541888.0
50	525526.5
51	509165.0
52	509165.0
53	509165.0
54	467238.5
55	425312.0
56	425312.0
57	425312.0
58	372247.0
59	319182.0
60	319182.0
61	319182.0
62	268126.0
63	217070.0
64	217070.0
65	217070.0
66	170430.0
67	123790.0
68	123790.0
69	123790.0
70	92778.0
71	61766.0
72	61766.0
73	61766.0
74	44837.5
75	27909.0
76	27909.0
77	27909.0
78	19576.0
79	11243.0
80	11243.0
81	11243.0
82	7775.5
83	4308.0
84	4308.0
85	4308.0
86	2820.0
87	1332.0
88	1332.0
89	1332.0
90	840.5
91	349.0
92	349.0
93	349.0
94	216.5
95	84.0
96	84.0
97	84.0
98	54.5
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08241951278800144
2	0.021206249450061814
3	0.03312817078268363
4	0.040682237750964356
5	0.04992436437696145
6	0.02145945839871927
7	0.02616492469460363
8	0.04256020412017381
9	0.0415895698169869
10	0.029203432078493085
11	0.02745207018361236
12	0.02196587629603418
13	0.030279570110287268
14	0.04462807720087636
15	0.041420763851215264
16	0.02846490597824218
17	0.027810782860877088
18	0.032874961834026176
19	0.022324588973298906
20	0.01979249948672436
21	0.01631087644268436
22	0.02378054042807927
23	0.024561268019773087
24	0.02342182775081454
25	0.10934406432857745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4739169.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.02965554259382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.35761428484165	56.16088089849829
2	12.428911799492948	17.159270005765563
3	3.0675315640893395	6.352519417053634
4	1.1705312237863779	3.2320546871929783
5	0.5531814252609023	1.9092961619160596
6	0.3443418669156037	1.4261880277246686
7	0.20712223922145118	1.0008303780067245
8	0.14970404718329217	0.8267215048315896
9	0.11222630645657408	0.6972248949763393
>10	0.5628979476785274	6.799257438892278
>50	0.030873343113702375	1.4663732284580608
>100	0.013957541003245809	1.626475079809052
>500	5.532057980797716E-4	0.28479459300904647
>1k	4.6100429859751926E-4	0.650673617727752
>5k	9.220085971937007E-5	0.40744006613797024
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6895	0.14548964174942908	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6543	0.13806217925547706	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5767	0.12168800057562836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	2.1100745721454542E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	85	3.9407314E-6	12.296181	4
TGTACCG	160	4.5474735E-11	11.283143	5
CGTACAC	125	2.5683312E-6	9.88145	3
GTACGAC	90	0.0011129054	9.501394	3
GCGCGCA	80	0.004524672	9.498385	13
GTCCTGG	1015	0.0	9.085042	1
GTTCTAG	535	0.0	9.0622835	1
TAATCGC	105	4.4882344E-4	9.049138	4
TACGACA	95	0.0018221092	9.001511	4
TACGACC	140	1.0839371E-5	8.822909	4
GGTATCA	4420	0.0	8.818259	1
GTCTAGA	455	0.0	8.775243	1
GACTGCG	185	6.60184E-8	8.728337	7
GTCTTAG	440	0.0	8.642285	1
TCTATAC	630	0.0	8.5964985	3
TCCTATA	410	0.0	8.3426	2
GTATTAG	770	0.0	8.2719	1
GGACTGT	725	0.0	8.253857	6
GTATAGA	750	0.0	8.238978	1
GTATTGG	625	0.0	8.213627	1
>>END_MODULE