FastQCFastQC Report
Wed 25 May 2016
SRR1295275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4739169
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT173260.3655915203699214No Hit
GGTATCAACGCAGAGTACTTTTTTT145300.3065938353327345No Hit
TATCAACGCAGAGTACTTTTTTTTT104850.2212413188894509No Hit
GTGGTATCAACGCAGAGTACATGGG74460.15711615264195053No Hit
ACGCAGAGTACTTTTTTTTTTTTTT71470.1508070296712356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3400.011.1851753
CCGTCCG700.001497150610.8529899
GGTATCA57100.010.3178461
TACGCAG1002.7363276E-49.5072985
TTATACC4450.09.4005754
GTGGTAT26050.09.2802671
CTTATAC5750.09.0940343
CGAGCCG1054.4963686E-49.04749919
AGGACCG2002.6248017E-99.0319345
GTCTTAT4800.08.92289451
CGTAGGC1502.7189108E-68.86327518
TATACTG11050.08.7759685
TGGTATC26900.08.6238122
TCTATAC5550.08.5652253
CTTAGAC3450.08.5428813
TTAGTAC2451.5279511E-108.5372563
CTATACT7050.08.4959744
TTATACT7600.08.3815224
TCGTAGA1252.7224023E-48.3665112
TAAGACG1252.7224023E-48.3665114
Produced by FastQC (version 0.11.5)