FastQCFastQC Report
Wed 25 May 2016
SRR1295274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254018
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52000.15980243502033484No Hit
GTGGTATCAACGCAGAGTACATGGG50140.1540864248446075No Hit
GGTATCAACGCAGAGTACTTTTTTT41810.12848730400385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG752.0695975E-411.4032864
GGTATCA27800.011.2864771
GTACTAG2300.09.9213941
CGTAGCA1151.0239199E-59.9157482
GTGGTAT12600.09.8853041
ACTAGAC2151.0913936E-119.7235813
TGGTATC12200.09.6583722
GTCTTAG3100.09.5080011
CTAGACA3250.09.3565424
ACTCCGT1151.1135648E-49.0852446
GACCGTG950.00182770588.9983047
CTACGTC850.0074208478.9437534
GCGTCAG1304.328416E-58.7766171
CGTCAGA1304.357438E-58.7716232
TGGACAG4600.08.6764135
GTCTCGC1554.16255E-68.58787251
GTATTAG5100.08.5758451
TAGACGG1000.0028986028.5524645
GCACCGA1000.00291113588.5483886
GTATTAA6800.08.5292371
Produced by FastQC (version 0.11.5)