##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295274_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3254018 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.745903987009292 31.0 30.0 33.0 25.0 34.0 2 29.739100705650674 31.0 30.0 33.0 25.0 34.0 3 30.128018038007166 31.0 30.0 34.0 26.0 34.0 4 33.503212028943906 35.0 33.0 37.0 27.0 37.0 5 33.70780493531382 35.0 35.0 37.0 28.0 37.0 6 33.87752772111279 35.0 35.0 37.0 28.0 37.0 7 33.78841696634745 35.0 35.0 37.0 28.0 37.0 8 33.7990020337933 35.0 35.0 37.0 28.0 37.0 9 34.79200115057753 38.0 34.0 39.0 27.0 39.0 10 34.69807788401908 38.0 34.0 39.0 27.0 39.0 11 35.0884340529155 38.0 35.0 39.0 27.0 39.0 12 35.095986254532086 38.0 35.0 39.0 27.0 39.0 13 34.992886947767346 38.0 35.0 39.0 27.0 39.0 14 35.11953775301796 38.0 33.0 40.0 24.0 41.0 15 35.744347757142094 39.0 34.0 41.0 27.0 41.0 16 35.833697908247586 39.0 34.0 41.0 27.0 41.0 17 35.65120076164299 39.0 34.0 41.0 26.0 41.0 18 35.62309550838379 39.0 34.0 41.0 26.0 41.0 19 35.69489566437555 39.0 34.0 41.0 26.0 41.0 20 35.66500492621737 39.0 34.0 41.0 26.0 41.0 21 35.65548193033966 39.0 34.0 41.0 26.0 41.0 22 35.389107866029015 39.0 34.0 40.0 25.0 41.0 23 35.05995111274738 39.0 33.0 40.0 24.0 41.0 24 35.39134848055542 39.0 34.0 40.0 25.0 41.0 25 33.99299143397486 38.0 32.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2754456649077781 1101 2 0.4247315192416181 1101 3 0.3167379366442482 1101 4 0.3120115231950962 1101 5 0.254474393742008 1101 6 0.21306313108449615 1101 7 0.123894113539599 1101 8 0.14464648659357948 1101 9 0.16973177518587335 1101 10 0.3840755470680577 1101 11 0.3638338109882895 1101 12 0.42090643482004 1101 13 0.30230892220128425 1101 14 0.7121775701081603 1101 15 0.5168275840856111 1101 16 0.32645778524460667 1101 17 0.5444634291267718 1101 18 0.6548286002576731 1101 19 0.7256475176298522 1101 20 0.7817399721717848 1101 21 0.7575540727611738 1101 22 0.5884087564746352 1101 23 -1.640805996114267 1101 24 -0.08600434560731429 1101 25 0.48671597235626507 1102 1 0.09159851283368425 1102 2 0.16770303173486667 1102 3 0.14880280007072955 1102 4 0.2778571131031171 1102 5 0.1364727771955927 1102 6 -0.027860326947198644 1102 7 -0.0438778094890111 1102 8 0.0921641960064008 1102 9 0.21157230337129818 1102 10 0.7539374880584901 1102 11 0.4530981337291209 1102 12 0.3334600107900201 1102 13 0.28718476915251756 1102 14 0.9179066483123535 1102 15 0.5866280571583644 1102 16 0.3797686872894701 1102 17 0.7079035950665826 1102 18 0.7421618769146008 1102 19 0.7710495959379102 1102 20 0.726786348637134 1102 21 0.6027344967792345 1102 22 0.5561675557130883 1102 23 0.5922523962323112 1102 24 0.6689388187741372 1102 25 0.46873569103223645 1103 1 -0.46525307711109676 1103 2 -1.6023129818573238 1103 3 -0.4377924036192731 1103 4 -0.14416166908331718 1103 5 -0.22852356824938624 1103 6 -0.1599834426875688 1103 7 -0.1344269151518276 1103 8 0.0567423773704121 1103 9 0.6999612637819155 1103 10 0.801888147640156 1103 11 0.4674649668530435 1103 12 0.37845315662665513 1103 13 0.07602616920190286 1103 14 -1.0126121270659993 1103 15 -0.2595085200731262 1103 16 -0.11967963059684905 1103 17 0.32760112891421755 1103 18 0.5414723605587639 1103 19 0.5467769255882544 1103 20 0.512486397795648 1103 21 0.6183834109927488 1103 22 0.23343156591845826 1103 23 0.8624474490749705 1103 24 0.8135784867795053 1103 25 0.4873858500056585 1104 1 -0.09452326586211868 1104 2 0.02955140385587285 1104 3 -0.3967625484946886 1104 4 -0.22155853721847052 1104 5 -0.18780508581144773 1104 6 -0.15064394822176297 1104 7 0.022398250891185967 1104 8 -0.09363894778373094 1104 9 -2.2819118356292805 1104 10 -0.650004671907368 1104 11 -0.5569722890414681 1104 12 -0.11777269407495794 1104 13 0.12396459577502128 1104 14 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0.2594828906714355 2213 8 0.1970915061971752 2213 9 0.6424023988955199 2213 10 0.7233232969142946 2213 11 0.1654598016812443 2213 12 0.22172246523212635 2213 13 0.23115466115200434 2213 14 -1.5925089368677376 2213 15 -0.2522437443443266 2213 16 0.015551916890395034 2213 17 -2.071864748495507 2213 18 -0.7892518882196882 2213 19 -0.3816388338629295 2213 20 0.3621899444173948 2213 21 -0.026459688153174454 2213 22 0.5419080774244094 2213 23 0.8887385392683811 2213 24 0.7822550113537048 2213 25 -0.45961708331122964 2214 1 0.37077034241781703 2214 2 0.247025696515891 2214 3 0.12684138437770187 2214 4 0.4365259912087538 2214 5 0.3983711091603297 2214 6 0.4276862486660775 2214 7 0.2529583942396556 2214 8 0.28281719675372585 2214 9 0.6204529535660015 2214 10 0.5435489033962781 2214 11 0.012528761441004121 2214 12 -0.06862748885932035 2214 13 0.10820107888112318 2214 14 0.915952155817898 2214 15 0.4849578611399963 2214 16 0.40206758327661873 2214 17 0.3889323463367589 2214 18 0.38656176590317415 2214 19 -0.8938346693793946 2214 20 0.11959293877213639 2214 21 0.43071370433082734 2214 22 0.6714407528779773 2214 23 1.1795562227644396 2214 24 0.9330243453265368 2214 25 1.0007791428195034 2215 1 -0.027315770185602162 2215 2 0.40540232943151366 2215 3 0.24203105376420808 2215 4 0.4152579611183427 2215 5 0.41321971962677395 2215 6 0.45010459893808275 2215 7 0.31730076910014304 2215 8 0.06383513200530899 2215 9 0.3981423955347836 2215 10 0.7797590069980203 2215 11 0.49990891981996555 2215 12 0.3834313519613559 2215 13 0.25946829553029715 2215 14 1.045671975682012 2215 15 0.5370647867125342 2215 16 0.5574862822307338 2215 17 0.658481737481253 2215 18 0.6163441801456457 2215 19 -0.10571453508490691 2215 20 -1.5057281337012682 2215 21 -1.6305427060296793 2215 22 0.01928469004182176 2215 23 0.6964255806435489 2215 24 0.7767003358942262 2215 25 0.15284920040843275 2216 1 0.24134422786816145 2216 2 -0.16008140205399712 2216 3 0.08723581555531013 2216 4 0.4212792821965081 2216 5 0.48583069945875224 2216 6 0.20296160321552037 2216 7 0.3253553867290009 2216 8 0.24334971133371397 2216 9 0.6057579012838303 2216 10 -1.2278844835459068 2216 11 -0.2132910749166257 2216 12 0.08979343267140649 2216 13 0.1846349802516727 2216 14 -0.34452932205356035 2216 15 -0.3331423007695591 2216 16 0.22762289515557654 2216 17 0.5926615458320725 2216 18 0.43743060833106284 2216 19 -1.2590578229876996 2216 20 -0.8255101093512849 2216 21 -0.6802281869244311 2216 22 -0.32504556603511503 2216 23 -4.267101617394545 2216 24 -1.8251598037818795 2216 25 -0.7563436593530142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 18.0 4 36.0 5 62.0 6 93.0 7 249.0 8 158.0 9 407.0 10 1279.0 11 2865.0 12 5649.0 13 9644.0 14 13853.0 15 17709.0 16 20576.0 17 22843.0 18 24038.0 19 24608.0 20 24886.0 21 25968.0 22 27154.0 23 28755.0 24 31084.0 25 34251.0 26 38457.0 27 44179.0 28 51506.0 29 62471.0 30 75971.0 31 93159.0 32 120833.0 33 158899.0 34 210850.0 35 284209.0 36 389605.0 37 560839.0 38 846851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47183360025196 20.808518923028707 13.460941659390096 23.258705817329236 2 15.98300113665913 20.92854416034455 40.194732024464294 22.89372267853202 3 18.171506108003406 26.185887790049083 29.95004345183732 25.69256265011019 4 12.856119553488258 17.371440341616527 38.07102869246827 31.701411412426943 5 13.68074109830375 37.57648802294871 35.04865532964178 13.694115549105756 6 30.98195865514893 38.311823633989626 17.31825871637688 13.387958994484567 7 26.180294449622167 32.90451166462154 23.29241842633964 17.62277545941666 8 23.744193689510073 37.06480532700262 21.11829178798854 18.07270919549877 9 24.689105009797995 17.31758111553888 21.947941191119025 36.0453726835441 10 15.44238365932688 29.884977266879854 33.38112242686624 21.291516646927025 11 32.05814429224979 25.633611283846115 23.608354438083904 18.69988998582019 12 21.130648122932563 27.608127555383156 30.916209558785106 20.345014762899172 13 26.49678031770155 24.289020127164232 26.70264447993862 22.511555075195595 14 21.335173906493555 23.470720436818432 27.72690239530715 27.467203261380863 15 22.85722718235516 30.893165182418493 24.078976534357626 22.17063110086872 16 22.207207844145017 30.927911107231143 25.548340730202895 21.31654031842094 17 20.60183463226511 30.07971262633 27.971410180665966 21.34704256073892 18 20.596226955665546 29.74645429804831 28.882550169488503 20.77476857679764 19 21.36915437565989 28.674384758075792 29.301575738574627 20.65488512768969 20 22.483831049916738 28.268506924017462 28.62320644680214 20.624455579263664 21 22.906260257855596 28.576925772159118 27.634586138940083 20.882227831045206 22 22.023187997741385 28.035075542806876 29.00193371634708 20.93980274310466 23 22.263229740085013 28.377636104265996 28.399982418030984 20.959151737618008 24 21.916430091609566 27.728399949344862 28.64987465312912 21.705295305916447 25 22.42295943685561 27.940419564843534 28.201459299798216 21.435161698502643 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3720.0 1 3720.0 2 3973.0 3 4226.0 4 4226.0 5 4226.0 6 5724.0 7 7222.0 8 7222.0 9 7222.0 10 10749.0 11 14276.0 12 14276.0 13 14276.0 14 22850.0 15 31424.0 16 31424.0 17 31424.0 18 48367.0 19 65310.0 20 65310.0 21 65310.0 22 92061.0 23 118812.0 24 118812.0 25 118812.0 26 152825.5 27 186839.0 28 186839.0 29 186839.0 30 223630.5 31 260422.0 32 260422.0 33 260422.0 34 292673.5 35 324925.0 36 324925.0 37 324925.0 38 349520.5 39 374116.0 40 374116.0 41 374116.0 42 378275.0 43 382434.0 44 382434.0 45 382434.0 46 373187.5 47 363941.0 48 363941.0 49 363941.0 50 350701.0 51 337461.0 52 337461.0 53 337461.0 54 310058.0 55 282655.0 56 282655.0 57 282655.0 58 246156.0 59 209657.0 60 209657.0 61 209657.0 62 175272.0 63 140887.0 64 140887.0 65 140887.0 66 110255.5 67 79624.0 68 79624.0 69 79624.0 70 59435.5 71 39247.0 72 39247.0 73 39247.0 74 28169.0 75 17091.0 76 17091.0 77 17091.0 78 11840.0 79 6589.0 80 6589.0 81 6589.0 82 4435.0 83 2281.0 84 2281.0 85 2281.0 86 1479.0 87 677.0 88 677.0 89 677.0 90 412.5 91 148.0 92 148.0 93 148.0 94 86.5 95 25.0 96 25.0 97 25.0 98 17.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08343530982311714 2 0.019176292202440184 3 0.030454656366375354 4 0.03835258440488037 5 0.04757195565605353 6 0.01850020497735415 7 0.023417202977979838 8 0.04016572741761109 9 0.04031938360513064 10 0.025660583315765307 11 0.024615721240632348 12 0.018192892602315044 13 0.027719576228527314 14 0.04326958240550605 15 0.04010426494260327 16 0.02581423950328486 17 0.025568389603253576 18 0.030577581316390996 19 0.019145560964936272 20 0.017209493002189907 21 0.012722732326618968 22 0.021419672540225653 23 0.021050897690178726 24 0.019852379427526216 25 0.109249549326402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3254018.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.5089822627033 #Duplication Level Percentage of deduplicated Percentage of total 1 88.54737385409949 69.51764203320455 2 8.147770784953147 12.793463840729174 3 1.5830452940760693 3.728498247410222 4 0.5792947230534375 1.8191935654831979 5 0.2999965129057881 1.177621045529668 6 0.18110141461616733 0.8530852648710693 7 0.12262546284614907 0.673904020128087 8 0.08578062550666975 0.5387639685109383 9 0.07148663310682647 0.5051108529543796 >10 0.3494914945396962 4.850993096505888 >50 0.022318305540590867 1.2031496147438403 >100 0.008693490681715156 1.0816673781526072 >500 6.285563536357603E-4 0.35995785447392004 >1k 3.1427817649662334E-4 0.5819282660086622 >5k 7.856954412415583E-5 0.31502095129385604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5200 0.15980243502033484 No Hit GTGGTATCAACGCAGAGTACATGGG 5014 0.1540864248446075 No Hit GGTATCAACGCAGAGTACTTTTTTT 4181 0.12848730400385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.073123750391055E-5 0.0 0.0 0.0 6 0.0 3.073123750391055E-5 0.0 0.0 0.0 7 0.0 3.073123750391055E-5 0.0 0.0 0.0 8 3.073123750391055E-5 3.073123750391055E-5 0.0 0.0 0.0 9 3.073123750391055E-5 3.073123750391055E-5 0.0 6.14624750078211E-5 0.0 10 3.073123750391055E-5 3.073123750391055E-5 0.0 6.14624750078211E-5 0.0 11 3.073123750391055E-5 3.073123750391055E-5 0.0 6.14624750078211E-5 0.0 12 3.073123750391055E-5 3.073123750391055E-5 0.0 6.14624750078211E-5 0.0 13 3.073123750391055E-5 3.073123750391055E-5 0.0 9.219371251173165E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGACG 75 2.0695975E-4 11.403286 4 GGTATCA 2780 0.0 11.286477 1 GTACTAG 230 0.0 9.921394 1 CGTAGCA 115 1.0239199E-5 9.915748 2 GTGGTAT 1260 0.0 9.885304 1 ACTAGAC 215 1.0913936E-11 9.723581 3 TGGTATC 1220 0.0 9.658372 2 GTCTTAG 310 0.0 9.508001 1 CTAGACA 325 0.0 9.356542 4 ACTCCGT 115 1.1135648E-4 9.085244 6 GACCGTG 95 0.0018277058 8.998304 7 CTACGTC 85 0.007420847 8.943753 4 GCGTCAG 130 4.328416E-5 8.776617 1 CGTCAGA 130 4.357438E-5 8.771623 2 TGGACAG 460 0.0 8.676413 5 GTCTCGC 155 4.16255E-6 8.5878725 1 GTATTAG 510 0.0 8.575845 1 TAGACGG 100 0.002898602 8.552464 5 GCACCGA 100 0.0029111358 8.548388 6 GTATTAA 680 0.0 8.529237 1 >>END_MODULE