FastQCFastQC Report
Wed 25 May 2016
SRR1295274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254018
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT136900.4207106414285355No Hit
GGTATCAACGCAGAGTACTTTTTTT114750.35264095035737353No Hit
TATCAACGCAGAGTACTTTTTTTTT81390.25012154204432796No Hit
GTGGTATCAACGCAGAGTACATGGG58390.1794396957853337No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58050.17839483371020073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT400.005294335514.244488
CCGCGTG500.001504548413.29484759
CAGCGAA550.003078605712.08622559
GTGGTAT15550.011.69339751
GGTATCA37550.011.4341631
GTTAGAC1001.9145245E-611.40838053
TGGTATC16050.011.0766732
AGCGTCA2600.010.9695973
TAACCCG1355.6346835E-810.5631525
AACCGTC856.6206115E-410.0549277
GACGCTC951.6515223E-49.9961268
AAGACCG1054.075614E-59.9595445
TGTACCG900.00110689439.5068385
CAGCGTC3150.09.356082
GTAGGAC2451.8189894E-129.31296353
GTCCTAG3050.09.051821
CGAATAG1054.5122512E-49.04411310
TAGGACC2002.6248017E-99.0314954
CTTAGAC2651.8189894E-128.9688533
GGACCGT850.00746028.9379876
Produced by FastQC (version 0.11.5)