FastQCFastQC Report
Wed 25 May 2016
SRR1295273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312589
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45750.1381094968316323No Hit
GTGGTATCAACGCAGAGTACATGGG41830.12627585251294382No Hit
GGTATCAACGCAGAGTACTTTTTTT38880.1173704314057675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC751.4828298E-512.6641298
GTACGGG1201.2713826E-711.0868054
TAGGACG700.001488279110.8605444
TTAACGT900.00111102579.5029764
GTCTTAC3150.09.35736751
GGTATCA32200.09.2425331
GCGTCAG1356.777129E-69.1561341
GTCCTAG3400.08.9489811
CGTGTTA850.0074506958.93938510
ACAGTGC6250.08.8142348
AGTACTG5400.08.7990535
ATAGGAC2304.0199666E-108.6766313
CTTAGAC2304.0199666E-108.6766313
GTCTTAG3750.08.6208521
TCTACAC3550.08.5660633
TAACCCG1000.00289796788.552685
CAGTGCG1451.6945332E-58.5155359
AGGACTG5700.08.3359445
GCTACCC1606.427066E-68.31510453
CTAGACT3100.08.2767864
Produced by FastQC (version 0.11.5)