##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295273_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3312589
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.155365486029204	31.0	30.0	33.0	26.0	34.0
2	30.170078449212987	31.0	30.0	34.0	26.0	34.0
3	30.43371695069929	31.0	30.0	34.0	26.0	34.0
4	33.59996033314124	35.0	35.0	37.0	28.0	37.0
5	34.002732605825834	35.0	35.0	37.0	28.0	37.0
6	34.182880218463566	37.0	35.0	37.0	30.0	37.0
7	34.09466945642819	37.0	35.0	37.0	29.0	37.0
8	34.04595951988007	37.0	35.0	37.0	29.0	37.0
9	35.03810282531277	38.0	35.0	39.0	27.0	39.0
10	34.95701126822555	38.0	35.0	39.0	27.0	39.0
11	35.39431242451146	39.0	35.0	39.0	28.0	39.0
12	35.38872283884297	39.0	35.0	39.0	27.0	39.0
13	35.30346565782836	39.0	35.0	39.0	27.0	39.0
14	35.511766174433355	39.0	34.0	41.0	25.0	41.0
15	36.1336607107009	39.0	35.0	41.0	27.0	41.0
16	36.21174948054226	39.0	36.0	41.0	27.0	41.0
17	36.021869601088454	39.0	35.0	41.0	27.0	41.0
18	35.998717015603205	39.0	35.0	41.0	27.0	41.0
19	36.07681091738215	39.0	35.0	41.0	27.0	41.0
20	36.05683349186995	39.0	35.0	41.0	27.0	41.0
21	36.04852548867366	39.0	35.0	41.0	27.0	41.0
22	35.78289941794771	39.0	34.0	41.0	26.0	41.0
23	35.456608109246275	39.0	34.0	41.0	25.0	41.0
24	35.79226369465092	39.0	34.0	41.0	27.0	41.0
25	34.47585740337845	38.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31954701970904154
1101	2	0.5490541141748473
1101	3	0.29321223992222656
1101	4	0.32742285227002554
1101	5	0.2342358564887519
1101	6	0.1659753617974289
1101	7	0.1914324349501797
1101	8	0.158110181733214
1101	9	0.19428388528382357
1101	10	0.4396361204953365
1101	11	0.40753060494343885
1101	12	0.38472317992853533
1101	13	0.3429054033426766
1101	14	0.822547175090385
1101	15	0.6001952921783626
1101	16	0.3658741658069502
1101	17	0.5814176606252204
1101	18	0.585666911027019
1101	19	0.5930108754431487
1101	20	0.7992549048764275
1101	21	0.799838092348061
1101	22	0.5633038322900035
1101	23	-1.5943136112967835
1101	24	0.05167551229362033
1101	25	0.41974853539929313
1102	1	0.266154347555819
1102	2	0.29668603642616276
1102	3	0.2115270396389768
1102	4	0.33887635596434507
1102	5	0.1250282664153204
1102	6	0.12692162534166584
1102	7	0.06661451966443366
1102	8	0.15927460568631346
1102	9	0.2898838935614947
1102	10	0.7527975030906617
1102	11	0.4764996145327913
1102	12	0.26903975358792565
1102	13	0.3736584134878953
1102	14	0.867764382334002
1102	15	0.49589852462144535
1102	16	0.26728772152997493
1102	17	0.6409771954915371
1102	18	0.6303957495591774
1102	19	0.7111607796264039
1102	20	0.7684353239155612
1102	21	0.752376903853083
1102	22	0.5632145801156483
1102	23	0.7249823593290117
1102	24	0.6722768533580776
1102	25	0.4648790601088919
1103	1	-0.44414274427873224
1103	2	-1.4779617090512716
1103	3	-0.4909662957029042
1103	4	-0.23435452731101236
1103	5	-0.275861593460192
1103	6	-0.09482588390459767
1103	7	-0.1461061615846191
1103	8	0.06571463567834002
1103	9	0.6001826308155174
1103	10	0.6831941314559344
1103	11	0.43259301421624485
1103	12	0.2401695922205036
1103	13	0.07172599597234353
1103	14	-1.0962825637532632
1103	15	-0.33231258950626597
1103	16	-0.2363213500647774
1103	17	0.23735593928185494
1103	18	0.43632923149091596
1103	19	0.3521331572887547
1103	20	0.4269985833440231
1103	21	0.4950984049749465
1103	22	0.059209898613538314
1103	23	0.8652606111105285
1103	24	0.7555667816603417
1103	25	0.5811007826531522
1104	1	-0.15037384006206622
1104	2	-0.04352378580738048
1104	3	-0.5582296226677208
1104	4	-0.5453732383297236
1104	5	-0.4210072639789928
1104	6	-0.23167948344476486
1104	7	-0.26255480639147066
1104	8	-0.23277398743714883
1104	9	-2.3898206494862677
1104	10	-0.7636678609284431
1104	11	-0.606771419924307
1104	12	-0.2965236123831545
1104	13	-0.11560361673040376
1104	14	-1.9521011837509903
1104	15	-0.8364225712281907
1104	16	-0.47451329795821806
1104	17	-0.07301114421955646
1104	18	0.08249955303436707
1104	19	0.09655923007303358
1104	20	0.23196558084833185
1104	21	0.34423917277243277
1104	22	0.5024145022658999
1104	23	0.8692729964662007
1104	24	0.5209084723751687
1104	25	0.2629927779779777
1105	1	-0.2858814351378669
1105	2	-0.1718991329756605
1105	3	-1.5435180233633865
1105	4	-0.6968969897553379
1105	5	-0.5002885640650021
1105	6	-0.25541006949134726
1105	7	-0.2652669816257287
1105	8	-0.23734637841778294
1105	9	0.13658479002025103
1105	10	0.19260825957731242
1105	11	-0.2298933010932913
1105	12	-0.24647662862665243
1105	13	-0.32993858818019106
1105	14	0.4479492101168745
1105	15	0.054477736060732695
1105	16	-0.4842254845595093
1105	17	0.1538090027183543
1105	18	0.07137747483672996
1105	19	-0.0179413212282995
1105	20	-0.6395308139061555
1105	21	-0.16301508134148435
1105	22	-0.11066346424680518
1105	23	0.20446667184633327
1105	24	-0.051560561919188785
1105	25	0.050240477100068404
1106	1	-1.534332351760007
1106	2	-1.0643312319815195
1106	3	-1.1063495902872589
1106	4	-1.451733567597735
1106	5	-0.924632077008468
1106	6	-0.8310770136015719
1106	7	-0.7345648603255981
1106	8	-0.7770139626520987
1106	9	-0.35901207066216045
1106	10	-1.8874703334366956
1106	11	-1.3731127273355028
1106	12	-0.9554252417334439
1106	13	-0.959226297712874
1106	14	-0.24726010453544944
1106	15	-1.0618157506765868
1106	16	-1.5092619995749104
1106	17	-0.679794718129962
1106	18	-0.6275670401210931
1106	19	-0.582870014282868
1106	20	-0.34244219312802926
1106	21	-0.35997711673289956
1106	22	-0.38952819230837576
1106	23	-0.6261550952517965
1106	24	-0.9500298948099655
1106	25	-1.592923319309989
1107	1	-0.019568068565316565
1107	2	0.14543900478689054
1107	3	0.022048873541674396
1107	4	0.0603745440015544
1107	5	-0.12208404682161245
1107	6	-0.015540339090648558
1107	7	-0.06129127249838007
1107	8	-0.043818607598851145
1107	9	0.4458662164309217
1107	10	-0.001291898456514673
1107	11	0.07614419379744675
1107	12	-0.18714048371019487
1107	13	0.11960266166205713
1107	14	-0.9442265144559698
1107	15	-0.36965981175909235
1107	16	-0.149754332796185
1107	17	0.06376325013641093
1107	18	0.3811785857825001
1107	19	0.18200895179914767
1107	20	0.266962134846807
1107	21	0.3806144979193533
1107	22	-0.28081013997985593
1107	23	0.5909409353126449
1107	24	0.487526722102821
1107	25	0.1795202459449854
1108	1	-0.1484391803479781
1108	2	0.1967176093727474
1108	3	-0.18983193210144478
1108	4	-0.44125846629813026
1108	5	-0.3992946012340255
1108	6	-0.34840495646433567
1108	7	-0.24522530777716156
1108	8	-0.3238728179529886
1108	9	0.21861656869844381
1108	10	0.17404488497219717
1108	11	0.007599242181221655
1108	12	-0.11578010031769281
1108	13	-0.2304229610335895
1108	14	-0.008484600216100091
1108	15	-0.17769129738366019
1108	16	-0.18361834220993956
1108	17	0.19765655281278782
1108	18	0.20820978132536538
1108	19	0.25883035483951744
1108	20	0.3087102370211383
1108	21	0.2894934212387099
1108	22	0.1849143194667917
1108	23	-0.0563778758798108
1108	24	0.014550647784886905
1108	25	-0.7142304534227506
1109	1	-0.8505804141126561
1109	2	-0.6612121869349536
1109	3	-0.7961695981945205
1109	4	-0.6960074804305236
1109	5	-0.8911268945669732
1109	6	-1.076251665980834
1109	7	-0.7627016915750673
1109	8	-1.2636035937281562
1109	9	-0.8379977254367148
1109	10	-1.0044696456179025
1109	11	-1.1556610077799618
1109	12	-1.021878776466238
1109	13	-1.0990321023418588
1109	14	-0.5295822369415077
1109	15	-1.1816537065962578
1109	16	-1.2965559214793103
1109	17	-1.4959511312397211
1109	18	-1.932414809915315
1109	19	-2.0354922356755907
1109	20	-1.7493721087251188
1109	21	-1.473586305868686
1109	22	-1.47706122413382
1109	23	-1.1027815418415656
1109	24	-2.039734726886806
1109	25	-1.026967156157383
1110	1	0.1677340988005902
1110	2	0.1819716802541258
1110	3	0.01095158990971612
1110	4	0.294658388494355
1110	5	0.19570785839650284
1110	6	0.11092702897306594
1110	7	0.17416498127171565
1110	8	0.12732165096037562
1110	9	0.4267234981899577
1110	10	0.17530410540201302
1110	11	0.18617917585647348
1110	12	0.1615055390422242
1110	13	0.22111867282207953
1110	14	-2.3034777747502204
1110	15	-0.5328657676713817
1110	16	-0.08436901253091378
1110	17	-0.11936246981616705
1110	18	-2.759925388447172
1110	19	-0.4568494837130359
1110	20	-1.7598631015463866
1110	21	-0.1697019062629863
1110	22	-1.6695328064738604
1110	23	-0.3859863427554018
1110	24	0.15195117888957554
1110	25	0.03126190473767565
1111	1	-0.07772204529971916
1111	2	-1.306820892069311
1111	3	-0.3469521184253921
1111	4	0.11353878540641915
1111	5	0.11886942866151884
1111	6	0.13294807967525912
1111	7	0.08434959980941414
1111	8	0.2037757795146291
1111	9	-1.4692921829779024
1111	10	-0.2864733794626062
1111	11	-0.10878109705961236
1111	12	0.0060320080116653685
1111	13	0.16791301603246467
1111	14	1.0117941766267862
1111	15	0.3092351799571986
1111	16	0.3676206715626833
1111	17	-0.019961070566466788
1111	18	0.3682313751983557
1111	19	0.11935810714327033
1111	20	0.5098035380832755
1111	21	-0.4639755718744709
1111	22	-0.07552904678317418
1111	23	0.05033639185120364
1111	24	-0.5434935794640694
1111	25	-2.0034590236496967
1112	1	0.12185609958488897
1112	2	0.2788262117620022
1112	3	0.0270022217018564
1112	4	0.32105591497466435
1112	5	0.15619086400128168
1112	6	0.13274418696898493
1112	7	0.12137083724420705
1112	8	0.02406415827928754
1112	9	-0.379216736039929
1112	10	0.4373284725173363
1112	11	0.10086816766568063
1112	12	-0.0020498859178204043
1112	13	0.14566009970310034
1112	14	0.5648750064796886
1112	15	0.1716132075057999
1112	16	0.19938750915510184
1112	17	-3.564357545943679
1112	18	-0.8375481313208297
1112	19	-1.103924242444016
1112	20	-0.06903539287297633
1112	21	-0.5023336523009618
1112	22	-1.6967030351767036
1112	23	-1.354463633279586
1112	24	-0.3534586456012576
1112	25	-0.06803416655525751
1113	1	-0.14221358025176656
1113	2	0.03173763941567387
1113	3	0.06620137559290029
1113	4	-0.29424590224459735
1113	5	-0.04221167198309672
1113	6	-0.010224391690364598
1113	7	-0.07779747478072352
1113	8	-0.09868188634581543
1113	9	0.32001379849092615
1113	10	0.4902930015895919
1113	11	0.19876510552779791
1113	12	0.1773338653116241
1113	13	-0.11634811191081695
1113	14	-1.3233670774267026
1113	15	-1.4971498207888985
1113	16	-0.15820596442141266
1113	17	-0.05751468075686006
1113	18	-0.4171181986938919
1113	19	0.05988858288819188
1113	20	0.4224530807372133
1113	21	-1.2102984697928392
1113	22	-1.660128426585544
1113	23	-0.35054998406284454
1113	24	-2.0551685639858306
1113	25	-0.36197870155988454
1114	1	0.25130483455300734
1114	2	0.30498144666266924
1114	3	0.11990842258805046
1114	4	0.5843862718086754
1114	5	0.39609609493762576
1114	6	0.4192993515665435
1114	7	0.4226756782977432
1114	8	0.3575011417419063
1114	9	0.7080819215556815
1114	10	0.5729205059034967
1114	11	0.4814499059830766
1114	12	0.5322978148821207
1114	13	0.3727141036576853
1114	14	1.1462415626296476
1114	15	0.5946436196485578
1114	16	0.621156787360043
1114	17	0.01540845651398115
1114	18	0.20746363368180454
1114	19	-0.1970573132052138
1114	20	-1.2695250069751367
1114	21	-0.3275799399803745
1114	22	-1.0520013576303384
1114	23	-2.4195099774994517
1114	24	-1.6644615100178157
1114	25	-1.0298531319179958
1115	1	0.48155355393963006
1115	2	0.44146103315576823
1115	3	0.40951411067198507
1115	4	0.6644606667828157
1115	5	0.6443263303406681
1115	6	0.40147957676537516
1115	7	0.5677019264581418
1115	8	0.6721054607859571
1115	9	0.8976461793505308
1115	10	1.0244529777341924
1115	11	0.694545116647646
1115	12	0.6167591971844857
1115	13	0.47724276791164755
1115	14	1.1934849596679769
1115	15	0.7365778949611368
1115	16	0.7418958190313916
1115	17	0.732128593040926
1115	18	1.021112729457144
1115	19	0.42703964414477724
1115	20	1.0408021097700058
1115	21	-0.14074654270864073
1115	22	0.8767256776606658
1115	23	1.092888324531991
1115	24	1.0850662834370013
1115	25	1.2606237293628055
1116	1	0.6317076154238457
1116	2	0.7261354101711142
1116	3	0.657197548236681
1116	4	1.0297030953863526
1116	5	0.7828023971368196
1116	6	0.6716917047425781
1116	7	0.6335855873294136
1116	8	0.6140258442645674
1116	9	0.9110244094365214
1116	10	0.7921429903386823
1116	11	0.717876665278169
1116	12	0.7042647341377162
1116	13	0.20778690222118001
1116	14	1.299996770556909
1116	15	0.889662044263936
1116	16	0.9984694948664483
1116	17	1.095967245486591
1116	18	1.0416671753282998
1116	19	1.2240014124780672
1116	20	1.1059156106756731
1116	21	0.5419121621486056
1116	22	0.6104768211779046
1116	23	-0.42525035823052093
1116	24	0.1597894947578098
1116	25	-0.26974704891311063
1201	1	0.3590922392835303
1201	2	0.6183648140330291
1201	3	0.42869628112565294
1201	4	0.36221637166952547
1201	5	0.3469188509350758
1201	6	0.1875868900384745
1201	7	0.21492648795729252
1201	8	0.31389495742456575
1201	9	0.4727041750867471
1201	10	0.7983366763772963
1201	11	0.38490559404186087
1201	12	0.4945515286919857
1201	13	0.46367542399442385
1201	14	1.018339414400451
1201	15	0.6070393071734514
1201	16	0.4805299817838602
1201	17	0.5953103747043684
1201	18	0.5111391485414387
1201	19	0.8349373937524049
1201	20	0.8948101280302296
1201	21	0.7818098892588097
1201	22	0.8574402482555001
1201	23	0.3363186665239155
1201	24	0.7689309155783306
1201	25	0.9899822326782797
1202	1	0.27269455764658446
1202	2	0.6557899858760443
1202	3	0.44312162045141434
1202	4	0.26680988480462275
1202	5	0.27163388345564954
1202	6	0.18784661819682924
1202	7	0.14331758494158464
1202	8	0.17533812526288983
1202	9	0.6339679614542746
1202	10	-0.09250752500451398
1202	11	0.2734089165912934
1202	12	0.23551727197505556
1202	13	0.22936383784273318
1202	14	0.8182135338819876
1202	15	0.5819017920597318
1202	16	0.5210514541286813
1202	17	-1.5297875992393344
1202	18	-0.03499788005827753
1202	19	0.5003342975558169
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2210	17	0.7075637294871342
2210	18	0.6589394149630436
2210	19	0.9001691615765921
2210	20	0.968140609083818
2210	21	1.0586083193110838
2210	22	1.172689241461626
2210	23	1.5560393263260792
2210	24	1.3062589653973689
2210	25	1.1948279994631008
2211	1	0.08034839917081271
2211	2	-0.5502611255234413
2211	3	-0.0389679182996332
2211	4	0.2711357271154142
2211	5	0.2448605657186249
2211	6	0.18346655510552523
2211	7	0.25081830698164254
2211	8	0.31284448264941034
2211	9	0.6729176760180167
2211	10	0.8190543334958775
2211	11	0.41830979558243797
2211	12	0.20690088146687202
2211	13	0.22163528008296396
2211	14	-0.8274736548681361
2211	15	-0.027385432403811194
2211	16	0.25778881625878824
2211	17	0.3314637368510702
2211	18	0.3273017606025377
2211	19	0.5535422700347965
2211	20	0.6618603554239613
2211	21	0.6018461718687931
2211	22	-0.03939075975213058
2211	23	0.6052568803224219
2211	24	-0.10056677463199293
2211	25	0.29322436352871506
2212	1	-0.0679396130063239
2212	2	-1.0971432846460019
2212	3	-0.34861197998235127
2212	4	0.20435218430406366
2212	5	0.23040920656098507
2212	6	0.05068142043008095
2212	7	0.07797547915873082
2212	8	0.10636238855181546
2212	9	-2.2615432827626876
2212	10	-0.019450649978168144
2212	11	0.051396679877257156
2212	12	-0.07292508499976691
2212	13	0.06408176016613254
2212	14	0.5331100946873804
2212	15	0.27232903752341997
2212	16	0.2855478730591301
2212	17	0.35344878184196205
2212	18	0.08464156414052582
2212	19	-0.4873112083790758
2212	20	0.39014060276837625
2212	21	0.34616736733011066
2212	22	-1.7992527027877614
2212	23	-0.15778710290465625
2212	24	0.3454096952827683
2212	25	-0.40204334522547214
2213	1	0.242917013784556
2213	2	0.41127482655587855
2213	3	0.10546844712838066
2213	4	0.3729844864347953
2213	5	0.3578218877177761
2213	6	0.2722068531316495
2213	7	0.23963975184322805
2213	8	0.3261666729122368
2213	9	0.7903662612486926
2213	10	0.7021108443909796
2213	11	0.07722433908109849
2213	12	0.1279956993418736
2213	13	0.1894320800459326
2213	14	-1.715139559834519
2213	15	-0.2877047791680596
2213	16	0.0342863187727005
2213	17	-1.9608588916196013
2213	18	-0.7349696639910022
2213	19	-0.35197810364376636
2213	20	0.3754408419615771
2213	21	0.008699682794500063
2213	22	0.49381292776313046
2213	23	1.0065544831687632
2213	24	0.86255366832372
2213	25	-0.7012024091041198
2214	1	0.3509539212424926
2214	2	0.2565408628651582
2214	3	0.293446017174972
2214	4	0.6085867541453496
2214	5	0.5475598944953575
2214	6	0.5046786015212064
2214	7	0.38680930747380415
2214	8	0.42988610387723725
2214	9	0.7200180423709881
2214	10	0.8067766472190812
2214	11	0.18231131544612822
2214	12	0.10343070056420345
2214	13	0.21777226826961282
2214	14	1.12296831730459
2214	15	0.7140758058363161
2214	16	0.7116045567211202
2214	17	0.695609913032591
2214	18	0.5665486501925159
2214	19	-0.5010274185576975
2214	20	0.3702186333705271
2214	21	0.7361692865122933
2214	22	0.9562608743257286
2214	23	1.4144062802305726
2214	24	1.1342840289061797
2214	25	1.1635801402822779
2215	1	-0.0759657822092592
2215	2	0.26103358038978186
2215	3	0.25472364293989
2215	4	0.5443441629095034
2215	5	0.41875773555793216
2215	6	0.3311335683576644
2215	7	0.32916885929994777
2215	8	0.2386704500906589
2215	9	0.5251925520192628
2215	10	0.8513013102538594
2215	11	0.5330431516834224
2215	12	0.3430012700634748
2215	13	0.1884341157383176
2215	14	1.0140900566439512
2215	15	0.5974324961306152
2215	16	0.563005016378817
2215	17	0.6188626800569423
2215	18	0.568912782172724
2215	19	-0.21900078249838373
2215	20	-1.4093977194217686
2215	21	-1.7957559231605345
2215	22	0.022459306915500576
2215	23	0.7150348501124455
2215	24	0.8360767668985574
2215	25	0.21238219885776743
2216	1	0.22164442932913175
2216	2	-0.13100257160141382
2216	3	0.13858058396544237
2216	4	0.5714048189965908
2216	5	0.48239693160110164
2216	6	0.325920622984583
2216	7	0.3859480164117528
2216	8	0.2925854872370053
2216	9	0.6207624186924576
2216	10	-1.178710377541492
2216	11	-0.17216075067629077
2216	12	0.18812443208785368
2216	13	0.2959453639219518
2216	14	-0.13006950044003673
2216	15	-0.052084222926389145
2216	16	0.3501365987362277
2216	17	0.6955617690706575
2216	18	0.4581743834493466
2216	19	-1.0545347945218495
2216	20	-0.6598407559608575
2216	21	-0.4568165578378398
2216	22	-0.5027116363553858
2216	23	-4.334071332606285
2216	24	-1.7733429463765376
2216	25	-0.7174508295358706
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	12.0
4	34.0
5	60.0
6	84.0
7	248.0
8	190.0
9	610.0
10	1524.0
11	3275.0
12	5741.0
13	9345.0
14	12985.0
15	16465.0
16	19336.0
17	20798.0
18	22259.0
19	22443.0
20	23160.0
21	23792.0
22	25064.0
23	26140.0
24	28065.0
25	31283.0
26	34787.0
27	40121.0
28	46884.0
29	56803.0
30	68928.0
31	85939.0
32	111690.0
33	148206.0
34	199826.0
35	274692.0
36	386747.0
37	578248.0
38	986803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.91345484516547	21.316667552908648	13.4955418000058	23.274335801920085
2	15.531187469635926	21.117089904226923	41.04872412769623	22.30299849844092
3	17.882692148574403	26.661122394932125	30.35458577103124	25.101599685462233
4	12.961550285901005	17.416405589852403	38.251120561618656	31.370923562627933
5	13.361069414703245	37.65464501339487	35.51448669121134	13.469798880690554
6	30.543596506880892	39.064679104479865	17.509073830682816	12.882650557956424
7	25.72194478438505	33.060283774672364	23.859543282869137	17.35822815807344
8	23.529127546125522	37.67460990688981	21.240615524363474	17.555647022621187
9	24.35946063148587	17.401465470485846	22.436634587857565	35.80243931017072
10	15.113629260889692	30.010003686840214	33.81206898165608	21.06429807061401
11	31.915994125277493	25.836429318537018	23.841662701067932	18.405913855117557
12	20.740800232366237	27.59783853505406	31.70446492812679	19.95689630445291
13	26.110753458327952	24.45644493670351	27.32486722895321	22.107934376015333
14	20.902035524666353	23.502498842611118	28.326809777210567	27.268655855511962
15	22.62358528228522	31.166852587908938	24.361833137144647	21.847728992661196
16	22.044701036904627	31.29896913442318	25.912439835979445	20.74388999269275
17	20.21132333896069	30.492780807519303	28.631314056990814	20.6645817965292
18	20.30622868924434	30.130856947532813	29.44459475291904	20.118319610303807
19	21.00514222722358	28.965555139222985	29.602000489735925	20.427302143817506
20	22.07402643671914	28.67402764442673	28.959197599077314	20.292748319776816
21	22.46043391361581	29.114601259593382	27.99521765121461	20.429747175576207
22	21.499159414198655	28.546130769063545	29.453746041637984	20.50096377509982
23	21.844099558356458	28.819935826267752	28.78503188607789	20.5509327292979
24	21.381161304971254	28.122927033699767	29.04080669544904	21.455104965879936
25	22.142959893429524	28.390799109333862	28.440633136938708	21.025607860297903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2593.0
1	2593.0
2	3006.5
3	3420.0
4	3420.0
5	3420.0
6	5212.5
7	7005.0
8	7005.0
9	7005.0
10	10586.0
11	14167.0
12	14167.0
13	14167.0
14	23645.5
15	33124.0
16	33124.0
17	33124.0
18	51806.5
19	70489.0
20	70489.0
21	70489.0
22	100008.0
23	129527.0
24	129527.0
25	129527.0
26	166438.0
27	203349.0
28	203349.0
29	203349.0
30	243313.5
31	283278.0
32	283278.0
33	283278.0
34	316933.0
35	350588.0
36	350588.0
37	350588.0
38	375991.0
39	401394.0
40	401394.0
41	401394.0
42	402430.5
43	403467.0
44	403467.0
45	403467.0
46	384350.0
47	365233.0
48	365233.0
49	365233.0
50	346150.5
51	327068.0
52	327068.0
53	327068.0
54	297085.0
55	267102.0
56	267102.0
57	267102.0
58	230983.5
59	194865.0
60	194865.0
61	194865.0
62	161174.0
63	127483.0
64	127483.0
65	127483.0
66	99142.0
67	70801.0
68	70801.0
69	70801.0
70	52521.0
71	34241.0
72	34241.0
73	34241.0
74	24441.5
75	14642.0
76	14642.0
77	14642.0
78	10187.0
79	5732.0
80	5732.0
81	5732.0
82	3926.5
83	2121.0
84	2121.0
85	2121.0
86	1404.5
87	688.0
88	688.0
89	688.0
90	432.0
91	176.0
92	176.0
93	176.0
94	100.5
95	25.0
96	25.0
97	25.0
98	18.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08250344368106034
2	0.0210711319756239
3	0.03208970385399457
4	0.03933479221237527
5	0.04887415855090988
6	0.017871217950672418
7	0.022218270965700847
8	0.03429341822966869
9	0.03504811493366669
10	0.0244521732095349
11	0.02333522208761787
12	0.017388212060113706
13	0.026354008903609834
14	0.037915962408859055
15	0.03474623625206749
16	0.024421985341374977
17	0.023908791582656345
18	0.026595511848889194
19	0.018112720895951777
20	0.015969382256597484
21	0.012346838077407126
22	0.018505163182030732
23	0.0193806113586684
24	0.018354223841231136
25	0.10882726471651025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3312589.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.28690262277003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.97214297437506	69.75038733525679
2	8.796163900650916	13.948411812896685
3	1.6891965326397604	4.017934829823884
4	0.5678195719951096	1.8008262044831684
5	0.26309956451296207	1.0430174775816214
6	0.16093825949252166	0.7656177665196995
7	0.10962523160747945	0.6084291544422584
8	0.08059149952289424	0.5111880299915785
9	0.053155171606392125	0.37930580235473793
>10	0.2812060394409367	3.8825054240550516
>50	0.01723426316184193	0.941151716581428
>100	0.00775674005176937	1.065703297174048
>500	5.733487221774675E-4	0.307969871358581
>1k	4.969022201754592E-4	0.9775512774804328
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4575	0.1381094968316323	No Hit
GTGGTATCAACGCAGAGTACATGGG	4183	0.12627585251294382	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3888	0.1173704314057675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.018786815991963E-5	0.0
4	0.0	0.0	0.0	3.018786815991963E-5	0.0
5	0.0	0.0	0.0	3.018786815991963E-5	0.0
6	0.0	0.0	0.0	3.018786815991963E-5	0.0
7	0.0	0.0	0.0	3.018786815991963E-5	0.0
8	0.0	0.0	0.0	3.018786815991963E-5	0.0
9	3.018786815991963E-5	0.0	0.0	9.056360447975888E-5	0.0
10	3.018786815991963E-5	0.0	0.0	9.056360447975888E-5	0.0
11	3.018786815991963E-5	0.0	0.0	9.056360447975888E-5	0.0
12	3.018786815991963E-5	0.0	0.0	9.056360447975888E-5	0.0
13	3.018786815991963E-5	0.0	0.0	1.2075147263967852E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATC	75	1.4828298E-5	12.664129	8
GTACGGG	120	1.2713826E-7	11.086805	4
TAGGACG	70	0.0014882791	10.860544	4
TTAACGT	90	0.0011110257	9.502976	4
GTCTTAC	315	0.0	9.3573675	1
GGTATCA	3220	0.0	9.242533	1
GCGTCAG	135	6.777129E-6	9.156134	1
GTCCTAG	340	0.0	8.948981	1
CGTGTTA	85	0.007450695	8.939385	10
ACAGTGC	625	0.0	8.814234	8
AGTACTG	540	0.0	8.799053	5
ATAGGAC	230	4.0199666E-10	8.676631	3
CTTAGAC	230	4.0199666E-10	8.676631	3
GTCTTAG	375	0.0	8.620852	1
TCTACAC	355	0.0	8.566063	3
TAACCCG	100	0.0028979678	8.55268	5
CAGTGCG	145	1.6945332E-5	8.515535	9
AGGACTG	570	0.0	8.335944	5
GCTACCC	160	6.427066E-6	8.3151045	3
CTAGACT	310	0.0	8.276786	4
>>END_MODULE