FastQCFastQC Report
Wed 25 May 2016
SRR1295273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312589
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106560.3216819231121036No Hit
GGTATCAACGCAGAGTACTTTTTTT88540.2672833846879284No Hit
TATCAACGCAGAGTACTTTTTTTTT64620.19507400404940065No Hit
GTGGTATCAACGCAGAGTACATGGG46340.13989058105306756No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44740.1350605221474804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2350.013.3504853
TAAATCG604.0698072E-412.6756435
CAGCGTC2650.011.8391082
GGACCGT1201.5323221E-610.2879216
GCAGCGT3000.010.1552451
GGTATCA37050.09.9445341
CTAGGAC3600.09.2430743
TAGTACG1151.1022122E-49.0938084
TTACGGG850.0073926218.9479183
AGGACCG1304.3352877E-58.7754445
ACACCGT1304.3902062E-58.766046
CTTAGAC2502.5465852E-118.7465893
GTCTTAC3050.08.74017051
GTCTTGG4700.08.7102841
GTCTAGG2850.08.6854081
TCTGTCG1752.643519E-78.6822918
AGGACGG1107.0975174E-48.6424845
CTACACT4950.08.4508114
TTAGACT2951.8189894E-128.3791944
CTGTCGC2053.8908183E-88.3381779
Produced by FastQC (version 0.11.5)